Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type

Autores
Barbagelata, María Sol; Álvarez, Lucía; Gordiola, Mariana Laura; Tuchscherr, Lorena Paola Nelly; von Eiff, Cristoff; Becker, Karsten; Sordelli, Daniel Oscar; Buzzola, Fernanda Roxana
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus aureus nasal carriage is a risk factor for infection in humans, particularly in the hospital setting. Bacterial interference was used as an alternative strategy for the prevention of upper respiratory, urogenital and gastrointestinal tract infections. This study was designed to assess if the administration of a live-attenuated aroA mutant of S. aureus is useful as a potential approach to prevent transient staphylococcal nasal carriage by virulent strains. We constructed an aroA mutant of S. aureus Newman strain by homologous recombination. The auxotrophic NK41 mutant was attenuated as determined by the increase of the LD50 after intraperitoneal challenge. In mice, previous nasal colonization with the NK41 mutant significantly reduced the number of CFU of S. aureus (HU-71 and Hde288) clinical isolates and the parental Newman strain. The NK41 mutant was unable to induce a pro-inflammatory response and to damage the invaded human respiratory epithelial cells. Moreover, the cells previously or simultaneously infected with the NK41 mutant were invaded by virulent strains in a significantly lower degree than those of the control group. In conclusion, the attenuated NK41 mutant interfered with the colonization and establishment of pathogenic strains of S. aureus, which produce severe infections. © 2011 Institut Pasteur.
Fil: Barbagelata, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Álvarez, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Gordiola, Mariana Laura. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Tuchscherr, Lorena Paola Nelly. Münster Universitätsklinikum Münster; Alemania
Fil: von Eiff, Cristoff. Münster Universitätsklinikum Münster; Alemania
Fil: Becker, Karsten. Münster Universitätsklinikum Münster; Alemania
Fil: Sordelli, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Materia
Bacterial Interference
Nasal Carriage
Staphylococcus Aureus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67497
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild typeBarbagelata, María SolÁlvarez, LucíaGordiola, Mariana LauraTuchscherr, Lorena Paola Nellyvon Eiff, CristoffBecker, KarstenSordelli, Daniel OscarBuzzola, Fernanda RoxanaBacterial InterferenceNasal CarriageStaphylococcus Aureushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Staphylococcus aureus nasal carriage is a risk factor for infection in humans, particularly in the hospital setting. Bacterial interference was used as an alternative strategy for the prevention of upper respiratory, urogenital and gastrointestinal tract infections. This study was designed to assess if the administration of a live-attenuated aroA mutant of S. aureus is useful as a potential approach to prevent transient staphylococcal nasal carriage by virulent strains. We constructed an aroA mutant of S. aureus Newman strain by homologous recombination. The auxotrophic NK41 mutant was attenuated as determined by the increase of the LD50 after intraperitoneal challenge. In mice, previous nasal colonization with the NK41 mutant significantly reduced the number of CFU of S. aureus (HU-71 and Hde288) clinical isolates and the parental Newman strain. The NK41 mutant was unable to induce a pro-inflammatory response and to damage the invaded human respiratory epithelial cells. Moreover, the cells previously or simultaneously infected with the NK41 mutant were invaded by virulent strains in a significantly lower degree than those of the control group. In conclusion, the attenuated NK41 mutant interfered with the colonization and establishment of pathogenic strains of S. aureus, which produce severe infections. © 2011 Institut Pasteur.Fil: Barbagelata, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; ArgentinaFil: Álvarez, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; ArgentinaFil: Gordiola, Mariana Laura. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; ArgentinaFil: Tuchscherr, Lorena Paola Nelly. Münster Universitätsklinikum Münster; AlemaniaFil: von Eiff, Cristoff. Münster Universitätsklinikum Münster; AlemaniaFil: Becker, Karsten. Münster Universitätsklinikum Münster; AlemaniaFil: Sordelli, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; ArgentinaFil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; ArgentinaElsevier Science2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67497Barbagelata, María Sol; Álvarez, Lucía; Gordiola, Mariana Laura; Tuchscherr, Lorena Paola Nelly; von Eiff, Cristoff; et al.; Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type; Elsevier Science; Microbes and Infection; 13; 12-13; 11-2011; 1081-10901286-4579CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2011.06.010info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1286457911001705info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:40:13Zoai:ri.conicet.gov.ar:11336/67497instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:40:13.521CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type
title Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type
spellingShingle Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type
Barbagelata, María Sol
Bacterial Interference
Nasal Carriage
Staphylococcus Aureus
title_short Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type
title_full Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type
title_fullStr Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type
title_full_unstemmed Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type
title_sort Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type
dc.creator.none.fl_str_mv Barbagelata, María Sol
Álvarez, Lucía
Gordiola, Mariana Laura
Tuchscherr, Lorena Paola Nelly
von Eiff, Cristoff
Becker, Karsten
Sordelli, Daniel Oscar
Buzzola, Fernanda Roxana
author Barbagelata, María Sol
author_facet Barbagelata, María Sol
Álvarez, Lucía
Gordiola, Mariana Laura
Tuchscherr, Lorena Paola Nelly
von Eiff, Cristoff
Becker, Karsten
Sordelli, Daniel Oscar
Buzzola, Fernanda Roxana
author_role author
author2 Álvarez, Lucía
Gordiola, Mariana Laura
Tuchscherr, Lorena Paola Nelly
von Eiff, Cristoff
Becker, Karsten
Sordelli, Daniel Oscar
Buzzola, Fernanda Roxana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacterial Interference
Nasal Carriage
Staphylococcus Aureus
topic Bacterial Interference
Nasal Carriage
Staphylococcus Aureus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Staphylococcus aureus nasal carriage is a risk factor for infection in humans, particularly in the hospital setting. Bacterial interference was used as an alternative strategy for the prevention of upper respiratory, urogenital and gastrointestinal tract infections. This study was designed to assess if the administration of a live-attenuated aroA mutant of S. aureus is useful as a potential approach to prevent transient staphylococcal nasal carriage by virulent strains. We constructed an aroA mutant of S. aureus Newman strain by homologous recombination. The auxotrophic NK41 mutant was attenuated as determined by the increase of the LD50 after intraperitoneal challenge. In mice, previous nasal colonization with the NK41 mutant significantly reduced the number of CFU of S. aureus (HU-71 and Hde288) clinical isolates and the parental Newman strain. The NK41 mutant was unable to induce a pro-inflammatory response and to damage the invaded human respiratory epithelial cells. Moreover, the cells previously or simultaneously infected with the NK41 mutant were invaded by virulent strains in a significantly lower degree than those of the control group. In conclusion, the attenuated NK41 mutant interfered with the colonization and establishment of pathogenic strains of S. aureus, which produce severe infections. © 2011 Institut Pasteur.
Fil: Barbagelata, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Álvarez, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Gordiola, Mariana Laura. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Tuchscherr, Lorena Paola Nelly. Münster Universitätsklinikum Münster; Alemania
Fil: von Eiff, Cristoff. Münster Universitätsklinikum Münster; Alemania
Fil: Becker, Karsten. Münster Universitätsklinikum Münster; Alemania
Fil: Sordelli, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
description Staphylococcus aureus nasal carriage is a risk factor for infection in humans, particularly in the hospital setting. Bacterial interference was used as an alternative strategy for the prevention of upper respiratory, urogenital and gastrointestinal tract infections. This study was designed to assess if the administration of a live-attenuated aroA mutant of S. aureus is useful as a potential approach to prevent transient staphylococcal nasal carriage by virulent strains. We constructed an aroA mutant of S. aureus Newman strain by homologous recombination. The auxotrophic NK41 mutant was attenuated as determined by the increase of the LD50 after intraperitoneal challenge. In mice, previous nasal colonization with the NK41 mutant significantly reduced the number of CFU of S. aureus (HU-71 and Hde288) clinical isolates and the parental Newman strain. The NK41 mutant was unable to induce a pro-inflammatory response and to damage the invaded human respiratory epithelial cells. Moreover, the cells previously or simultaneously infected with the NK41 mutant were invaded by virulent strains in a significantly lower degree than those of the control group. In conclusion, the attenuated NK41 mutant interfered with the colonization and establishment of pathogenic strains of S. aureus, which produce severe infections. © 2011 Institut Pasteur.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67497
Barbagelata, María Sol; Álvarez, Lucía; Gordiola, Mariana Laura; Tuchscherr, Lorena Paola Nelly; von Eiff, Cristoff; et al.; Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type; Elsevier Science; Microbes and Infection; 13; 12-13; 11-2011; 1081-1090
1286-4579
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67497
identifier_str_mv Barbagelata, María Sol; Álvarez, Lucía; Gordiola, Mariana Laura; Tuchscherr, Lorena Paola Nelly; von Eiff, Cristoff; et al.; Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type; Elsevier Science; Microbes and Infection; 13; 12-13; 11-2011; 1081-1090
1286-4579
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2011.06.010
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1286457911001705
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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