Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)

Autores
Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We performed karyotype analyses on Hylocereineae species. Epiphyllum phyllanthus, Selenicereus aff. megalanthus and S. aff. setaceuswere diploid, with 2n= 22 chromosomes, whereas S. setaceus was tetraploid. Karyotypes were symmetrical with the formulas 11 min E. phyllanthus, S. aff. setaceusand S. aff. megalanthus, and 22 min S. setaceus. Chromosomes were small with the average length being 2.76 µm. Diploid species had two terminal satellites and tetraploids had four terminal satellites. The only bands associated with NOR were found in all specimens and were CMA + /DAPI − bands. Pericentromeric heterochromatin was found in S. aff. megalanthus. The locations of the 18−5.8−26S rDNA sites in all species coincided with CMA+ bands. The signals of the 5S rDNA gene were located in paracentromeric regions on at least one locus in all taxa. Additional 5S loci were found in all species, with the exception of E. phyllanthus. One pair of 5S sites was co-localized with 18−5.8−26S loci in S. aff. setaceusand tetraploid S. setaceus, which is a new position for Cactaceae. The 18−5.8−26S rDNA loci seem to be highly conserved, in contrast with the 5S rDNA loci that were scattered throughout the genome.
Fil: Moreno, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Bernardello, Gabriel Luis Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Materia
Cactaceae
Epifitas
Cariotipo
Cromosomas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14729
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14729
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)Moreno, Natalia CeciliaLas Peñas, Maria LauraBernardello, Gabriel Luis MarioCactaceaeEpifitasCariotipoCromosomashttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We performed karyotype analyses on Hylocereineae species. Epiphyllum phyllanthus, Selenicereus aff. megalanthus and S. aff. setaceuswere diploid, with 2n= 22 chromosomes, whereas S. setaceus was tetraploid. Karyotypes were symmetrical with the formulas 11 min E. phyllanthus, S. aff. setaceusand S. aff. megalanthus, and 22 min S. setaceus. Chromosomes were small with the average length being 2.76 µm. Diploid species had two terminal satellites and tetraploids had four terminal satellites. The only bands associated with NOR were found in all specimens and were CMA + /DAPI − bands. Pericentromeric heterochromatin was found in S. aff. megalanthus. The locations of the 18−5.8−26S rDNA sites in all species coincided with CMA+ bands. The signals of the 5S rDNA gene were located in paracentromeric regions on at least one locus in all taxa. Additional 5S loci were found in all species, with the exception of E. phyllanthus. One pair of 5S sites was co-localized with 18−5.8−26S loci in S. aff. setaceusand tetraploid S. setaceus, which is a new position for Cactaceae. The 18−5.8−26S rDNA loci seem to be highly conserved, in contrast with the 5S rDNA loci that were scattered throughout the genome.Fil: Moreno, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Bernardello, Gabriel Luis Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; ArgentinaCactus Succulent Soc Amer Inc2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14729Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario; Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae); Cactus Succulent Soc Amer Inc; Haseltonia; 20; 5-2014; 26-331070-0048enginfo:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abs/10.2985/026.020.0106info:eu-repo/semantics/altIdentifier/doi/10.2985/026.020.0106info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:33Zoai:ri.conicet.gov.ar:11336/14729instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:33.351CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)
title Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)
spellingShingle Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)
Moreno, Natalia Cecilia
Cactaceae
Epifitas
Cariotipo
Cromosomas
title_short Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)
title_full Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)
title_fullStr Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)
title_full_unstemmed Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)
title_sort Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)
dc.creator.none.fl_str_mv Moreno, Natalia Cecilia
Las Peñas, Maria Laura
Bernardello, Gabriel Luis Mario
author Moreno, Natalia Cecilia
author_facet Moreno, Natalia Cecilia
Las Peñas, Maria Laura
Bernardello, Gabriel Luis Mario
author_role author
author2 Las Peñas, Maria Laura
Bernardello, Gabriel Luis Mario
author2_role author
author
dc.subject.none.fl_str_mv Cactaceae
Epifitas
Cariotipo
Cromosomas
topic Cactaceae
Epifitas
Cariotipo
Cromosomas
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We performed karyotype analyses on Hylocereineae species. Epiphyllum phyllanthus, Selenicereus aff. megalanthus and S. aff. setaceuswere diploid, with 2n= 22 chromosomes, whereas S. setaceus was tetraploid. Karyotypes were symmetrical with the formulas 11 min E. phyllanthus, S. aff. setaceusand S. aff. megalanthus, and 22 min S. setaceus. Chromosomes were small with the average length being 2.76 µm. Diploid species had two terminal satellites and tetraploids had four terminal satellites. The only bands associated with NOR were found in all specimens and were CMA + /DAPI − bands. Pericentromeric heterochromatin was found in S. aff. megalanthus. The locations of the 18−5.8−26S rDNA sites in all species coincided with CMA+ bands. The signals of the 5S rDNA gene were located in paracentromeric regions on at least one locus in all taxa. Additional 5S loci were found in all species, with the exception of E. phyllanthus. One pair of 5S sites was co-localized with 18−5.8−26S loci in S. aff. setaceusand tetraploid S. setaceus, which is a new position for Cactaceae. The 18−5.8−26S rDNA loci seem to be highly conserved, in contrast with the 5S rDNA loci that were scattered throughout the genome.
Fil: Moreno, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Bernardello, Gabriel Luis Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
description We performed karyotype analyses on Hylocereineae species. Epiphyllum phyllanthus, Selenicereus aff. megalanthus and S. aff. setaceuswere diploid, with 2n= 22 chromosomes, whereas S. setaceus was tetraploid. Karyotypes were symmetrical with the formulas 11 min E. phyllanthus, S. aff. setaceusand S. aff. megalanthus, and 22 min S. setaceus. Chromosomes were small with the average length being 2.76 µm. Diploid species had two terminal satellites and tetraploids had four terminal satellites. The only bands associated with NOR were found in all specimens and were CMA + /DAPI − bands. Pericentromeric heterochromatin was found in S. aff. megalanthus. The locations of the 18−5.8−26S rDNA sites in all species coincided with CMA+ bands. The signals of the 5S rDNA gene were located in paracentromeric regions on at least one locus in all taxa. Additional 5S loci were found in all species, with the exception of E. phyllanthus. One pair of 5S sites was co-localized with 18−5.8−26S loci in S. aff. setaceusand tetraploid S. setaceus, which is a new position for Cactaceae. The 18−5.8−26S rDNA loci seem to be highly conserved, in contrast with the 5S rDNA loci that were scattered throughout the genome.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14729
Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario; Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae); Cactus Succulent Soc Amer Inc; Haseltonia; 20; 5-2014; 26-33
1070-0048
url http://hdl.handle.net/11336/14729
identifier_str_mv Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario; Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae); Cactus Succulent Soc Amer Inc; Haseltonia; 20; 5-2014; 26-33
1070-0048
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abs/10.2985/026.020.0106
info:eu-repo/semantics/altIdentifier/doi/10.2985/026.020.0106
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cactus Succulent Soc Amer Inc
publisher.none.fl_str_mv Cactus Succulent Soc Amer Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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