Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis

Autores
Filippa, Veronica Palmira; Mohamed, Fabian
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The neuroendocrine hypothalamic-pituitary axis undergoes morphological and biochemical changes throughout gestation. In viscacha, pregnancy lasts approximately 154 days, and three stages can be described: early, mid- and late pregnancy. The aim of this work was to study the pituitary LH-gonadotrophs, FSH-gonadotrophs, somatotrophs, corticotrophs and thyrotrophs of non-pregnant and pregnant adult viscachas by immunohistochemistry and morphometric analysis. Immunopositive percentage area (%IA), cell percentage in the pars distalis (%PDC), number of cells per reference area (n°cell/RA), and major cellular (MCD) and nuclear (ND) diameters were analyzed. The different cell populations showed a well-defined morphology, immunolabeling patterns and regionalization in the pars distalis (PD). In the early pregnancy of animals the morphometric analysis demonstrated that %IA, %PDC and n°cell/RA increased in the FSH-gonadotrophs and decreased in the somatotrophs in relation to non-pregnant animals. In mid-pregnancy, there was an increase in %IA, %PDC, and n°cell/RA of LH-gonadotrophs, FSH-gonadotrophs, somatotrophs, and thyrotrophs. The MCD of LH-gonadotrophs and FSH-gonadotrophs increased. In late pregnancy, the %IA, %PDC and n°cell/RA of LH-gonadotrophs, FSH-gonadotrophs, somatotrophs and corticotrophs decreased whereas the values of the thyrotrophs remained constant. The MCD of LH-gonadotrophs, FSH-gonadotrophs and corticotrophs decreased. No significant changes were observed in the ND of the studied cell types. In conclusion, this work provides evidence for histological and morphometric changes in the different cell types of the pituitary PD in viscachas during pregnancy, probably according to the requirements of this physiological stage.
Fil: Filippa, Veronica Palmira. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis; Argentina
Fil: Mohamed, Fabian. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Materia
Hormonal
Pars Distalis
Pituitary
Pregnancy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13978
Acceder
id CONICETDig_815b4e5dbd00492947309b4dcd3ed787
oai_identifier_str oai:ri.conicet.gov.ar:11336/13978
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysisFilippa, Veronica PalmiraMohamed, FabianHormonalPars DistalisPituitaryPregnancyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The neuroendocrine hypothalamic-pituitary axis undergoes morphological and biochemical changes throughout gestation. In viscacha, pregnancy lasts approximately 154 days, and three stages can be described: early, mid- and late pregnancy. The aim of this work was to study the pituitary LH-gonadotrophs, FSH-gonadotrophs, somatotrophs, corticotrophs and thyrotrophs of non-pregnant and pregnant adult viscachas by immunohistochemistry and morphometric analysis. Immunopositive percentage area (%IA), cell percentage in the pars distalis (%PDC), number of cells per reference area (n°cell/RA), and major cellular (MCD) and nuclear (ND) diameters were analyzed. The different cell populations showed a well-defined morphology, immunolabeling patterns and regionalization in the pars distalis (PD). In the early pregnancy of animals the morphometric analysis demonstrated that %IA, %PDC and n°cell/RA increased in the FSH-gonadotrophs and decreased in the somatotrophs in relation to non-pregnant animals. In mid-pregnancy, there was an increase in %IA, %PDC, and n°cell/RA of LH-gonadotrophs, FSH-gonadotrophs, somatotrophs, and thyrotrophs. The MCD of LH-gonadotrophs and FSH-gonadotrophs increased. In late pregnancy, the %IA, %PDC and n°cell/RA of LH-gonadotrophs, FSH-gonadotrophs, somatotrophs and corticotrophs decreased whereas the values of the thyrotrophs remained constant. The MCD of LH-gonadotrophs, FSH-gonadotrophs and corticotrophs decreased. No significant changes were observed in the ND of the studied cell types. In conclusion, this work provides evidence for histological and morphometric changes in the different cell types of the pituitary PD in viscachas during pregnancy, probably according to the requirements of this physiological stage.Fil: Filippa, Veronica Palmira. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis; ArgentinaFil: Mohamed, Fabian. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaElsevier Gmbh2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13978Filippa, Veronica Palmira; Mohamed, Fabian; Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis; Elsevier Gmbh; Zoology; 113; 6; 12-2010; 361-3720944-2006enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944200610000589info:eu-repo/semantics/altIdentifier/doi/10.1016/j.zool.2010.08.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:34Zoai:ri.conicet.gov.ar:11336/13978instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:34.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis
title Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis
spellingShingle Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis
Filippa, Veronica Palmira
Hormonal
Pars Distalis
Pituitary
Pregnancy
title_short Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis
title_full Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis
title_fullStr Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis
title_full_unstemmed Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis
title_sort Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis
dc.creator.none.fl_str_mv Filippa, Veronica Palmira
Mohamed, Fabian
author Filippa, Veronica Palmira
author_facet Filippa, Veronica Palmira
Mohamed, Fabian
author_role author
author2 Mohamed, Fabian
author2_role author
dc.subject.none.fl_str_mv Hormonal
Pars Distalis
Pituitary
Pregnancy
topic Hormonal
Pars Distalis
Pituitary
Pregnancy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The neuroendocrine hypothalamic-pituitary axis undergoes morphological and biochemical changes throughout gestation. In viscacha, pregnancy lasts approximately 154 days, and three stages can be described: early, mid- and late pregnancy. The aim of this work was to study the pituitary LH-gonadotrophs, FSH-gonadotrophs, somatotrophs, corticotrophs and thyrotrophs of non-pregnant and pregnant adult viscachas by immunohistochemistry and morphometric analysis. Immunopositive percentage area (%IA), cell percentage in the pars distalis (%PDC), number of cells per reference area (n°cell/RA), and major cellular (MCD) and nuclear (ND) diameters were analyzed. The different cell populations showed a well-defined morphology, immunolabeling patterns and regionalization in the pars distalis (PD). In the early pregnancy of animals the morphometric analysis demonstrated that %IA, %PDC and n°cell/RA increased in the FSH-gonadotrophs and decreased in the somatotrophs in relation to non-pregnant animals. In mid-pregnancy, there was an increase in %IA, %PDC, and n°cell/RA of LH-gonadotrophs, FSH-gonadotrophs, somatotrophs, and thyrotrophs. The MCD of LH-gonadotrophs and FSH-gonadotrophs increased. In late pregnancy, the %IA, %PDC and n°cell/RA of LH-gonadotrophs, FSH-gonadotrophs, somatotrophs and corticotrophs decreased whereas the values of the thyrotrophs remained constant. The MCD of LH-gonadotrophs, FSH-gonadotrophs and corticotrophs decreased. No significant changes were observed in the ND of the studied cell types. In conclusion, this work provides evidence for histological and morphometric changes in the different cell types of the pituitary PD in viscachas during pregnancy, probably according to the requirements of this physiological stage.
Fil: Filippa, Veronica Palmira. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis; Argentina
Fil: Mohamed, Fabian. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
description The neuroendocrine hypothalamic-pituitary axis undergoes morphological and biochemical changes throughout gestation. In viscacha, pregnancy lasts approximately 154 days, and three stages can be described: early, mid- and late pregnancy. The aim of this work was to study the pituitary LH-gonadotrophs, FSH-gonadotrophs, somatotrophs, corticotrophs and thyrotrophs of non-pregnant and pregnant adult viscachas by immunohistochemistry and morphometric analysis. Immunopositive percentage area (%IA), cell percentage in the pars distalis (%PDC), number of cells per reference area (n°cell/RA), and major cellular (MCD) and nuclear (ND) diameters were analyzed. The different cell populations showed a well-defined morphology, immunolabeling patterns and regionalization in the pars distalis (PD). In the early pregnancy of animals the morphometric analysis demonstrated that %IA, %PDC and n°cell/RA increased in the FSH-gonadotrophs and decreased in the somatotrophs in relation to non-pregnant animals. In mid-pregnancy, there was an increase in %IA, %PDC, and n°cell/RA of LH-gonadotrophs, FSH-gonadotrophs, somatotrophs, and thyrotrophs. The MCD of LH-gonadotrophs and FSH-gonadotrophs increased. In late pregnancy, the %IA, %PDC and n°cell/RA of LH-gonadotrophs, FSH-gonadotrophs, somatotrophs and corticotrophs decreased whereas the values of the thyrotrophs remained constant. The MCD of LH-gonadotrophs, FSH-gonadotrophs and corticotrophs decreased. No significant changes were observed in the ND of the studied cell types. In conclusion, this work provides evidence for histological and morphometric changes in the different cell types of the pituitary PD in viscachas during pregnancy, probably according to the requirements of this physiological stage.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13978
Filippa, Veronica Palmira; Mohamed, Fabian; Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis; Elsevier Gmbh; Zoology; 113; 6; 12-2010; 361-372
0944-2006
url http://hdl.handle.net/11336/13978
identifier_str_mv Filippa, Veronica Palmira; Mohamed, Fabian; Pituitary of non-pregnant and pregnant viscachas (Lagostomus maximus maximus): a comparative study by immunohistochemical and morphometric analysis; Elsevier Gmbh; Zoology; 113; 6; 12-2010; 361-372
0944-2006
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944200610000589
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.zool.2010.08.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614471331348480
score 13.070432

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