Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials

Autores
Kozuszko, Silvia Noemi; Sanchez, Maria Alejandra; de Ferro, María Inés Gutiérrez; Sfer, Ana María; Moreno Madrid, Ana Paula; Takabatake, Kiyofumi; Nakano, Keisuke; Nagatsuka, Hitoshi; Rodriguez, Andrea Paola
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work is to evaluate the antibacterial effect and biocompatibility of zinc oxide (ZnO) nanopaticles and graphite-type carbon (Gt) microparticles commercial powders. SEM analysis was performed to assess particles morphology. The antibacterial behavior was studied against Staphylococcus aureus (Staph. Aureus) (bacterial strain ATCC 29213) and TEM analysis of bacteria was performed to determine ultrastructural alterations; in addition, biocompatibility was evaluated in subcutaneous tissue of Wistar rats at 3, 7 and 28 d. ZnO and Gt powders exhibited antibacterial activity while TEM images of Staph. aureus showed membrane disruption followed by the release of internal content. Also, an electron-light region within the cytoplasm was observed for microorganisms treated with ZnO. Regarding biocompatibility, Gt samples induced a foreign body reaction response with presence of giant cells whereas ZnO samples showed fibroblastic connective tissue with chronic inflammatory cells and new small vessels. Also, collagen fibers and lack of capsule was observed by Trichome Masson stain. Thus, ZnO improved wound healing by enhancing tissue regeneration in contrast with calcium hydroxide control sample response which showed a fibrous tissue scar. Hence, ZnO nano-powder seems to be a potential material in the regenerative endodontic field.
Fil: Kozuszko, Silvia Noemi. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; Argentina
Fil: Sanchez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; Argentina
Fil: de Ferro, María Inés Gutiérrez. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Sfer, Ana María. Universidad Nacioal de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Estadística; Argentina
Fil: Moreno Madrid, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; Argentina
Fil: Takabatake, Kiyofumi. Okayama University; Japón
Fil: Nakano, Keisuke. Okayama University; Japón
Fil: Nagatsuka, Hitoshi. Okayama University; Japón
Fil: Rodriguez, Andrea Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; Argentina
Materia
ANTIBACTERIAL AGENTS
FOREIGN BODY REACTION
GRAPHITE
STAPHYLOCOCCUS AUREUS
ZINC OXIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53856
Acceder
id CONICETDig_80c87cd499d5a36bd11fd0bce7042e54
oai_identifier_str oai:ri.conicet.gov.ar:11336/53856
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materialsKozuszko, Silvia NoemiSanchez, Maria Alejandrade Ferro, María Inés GutiérrezSfer, Ana MaríaMoreno Madrid, Ana PaulaTakabatake, KiyofumiNakano, KeisukeNagatsuka, HitoshiRodriguez, Andrea PaolaANTIBACTERIAL AGENTSFOREIGN BODY REACTIONGRAPHITESTAPHYLOCOCCUS AUREUSZINC OXIDEhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2The aim of this work is to evaluate the antibacterial effect and biocompatibility of zinc oxide (ZnO) nanopaticles and graphite-type carbon (Gt) microparticles commercial powders. SEM analysis was performed to assess particles morphology. The antibacterial behavior was studied against Staphylococcus aureus (Staph. Aureus) (bacterial strain ATCC 29213) and TEM analysis of bacteria was performed to determine ultrastructural alterations; in addition, biocompatibility was evaluated in subcutaneous tissue of Wistar rats at 3, 7 and 28 d. ZnO and Gt powders exhibited antibacterial activity while TEM images of Staph. aureus showed membrane disruption followed by the release of internal content. Also, an electron-light region within the cytoplasm was observed for microorganisms treated with ZnO. Regarding biocompatibility, Gt samples induced a foreign body reaction response with presence of giant cells whereas ZnO samples showed fibroblastic connective tissue with chronic inflammatory cells and new small vessels. Also, collagen fibers and lack of capsule was observed by Trichome Masson stain. Thus, ZnO improved wound healing by enhancing tissue regeneration in contrast with calcium hydroxide control sample response which showed a fibrous tissue scar. Hence, ZnO nano-powder seems to be a potential material in the regenerative endodontic field.Fil: Kozuszko, Silvia Noemi. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; ArgentinaFil: Sanchez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; ArgentinaFil: de Ferro, María Inés Gutiérrez. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Sfer, Ana María. Universidad Nacioal de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Estadística; ArgentinaFil: Moreno Madrid, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; ArgentinaFil: Takabatake, Kiyofumi. Okayama University; JapónFil: Nakano, Keisuke. Okayama University; JapónFil: Nagatsuka, Hitoshi. Okayama University; JapónFil: Rodriguez, Andrea Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; ArgentinaThe Society for Hard Tissue Regenerative Biology2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53856Kozuszko, Silvia Noemi; Sanchez, Maria Alejandra; de Ferro, María Inés Gutiérrez; Sfer, Ana María; Moreno Madrid, Ana Paula; et al.; Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials; The Society for Hard Tissue Regenerative Biology; Journal of Hard Tissue Biology; 26; 4; 10-2017; 311-3181341-76491880-828XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2485/jhtb.26.311info:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/jhtb/26/4/26_311/_article/-char/eninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:34:55Zoai:ri.conicet.gov.ar:11336/53856instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:34:55.323CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials
title Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials
spellingShingle Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials
Kozuszko, Silvia Noemi
ANTIBACTERIAL AGENTS
FOREIGN BODY REACTION
GRAPHITE
STAPHYLOCOCCUS AUREUS
ZINC OXIDE
title_short Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials
title_full Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials
title_fullStr Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials
title_full_unstemmed Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials
title_sort Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials
dc.creator.none.fl_str_mv Kozuszko, Silvia Noemi
Sanchez, Maria Alejandra
de Ferro, María Inés Gutiérrez
Sfer, Ana María
Moreno Madrid, Ana Paula
Takabatake, Kiyofumi
Nakano, Keisuke
Nagatsuka, Hitoshi
Rodriguez, Andrea Paola
author Kozuszko, Silvia Noemi
author_facet Kozuszko, Silvia Noemi
Sanchez, Maria Alejandra
de Ferro, María Inés Gutiérrez
Sfer, Ana María
Moreno Madrid, Ana Paula
Takabatake, Kiyofumi
Nakano, Keisuke
Nagatsuka, Hitoshi
Rodriguez, Andrea Paola
author_role author
author2 Sanchez, Maria Alejandra
de Ferro, María Inés Gutiérrez
Sfer, Ana María
Moreno Madrid, Ana Paula
Takabatake, Kiyofumi
Nakano, Keisuke
Nagatsuka, Hitoshi
Rodriguez, Andrea Paola
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBACTERIAL AGENTS
FOREIGN BODY REACTION
GRAPHITE
STAPHYLOCOCCUS AUREUS
ZINC OXIDE
topic ANTIBACTERIAL AGENTS
FOREIGN BODY REACTION
GRAPHITE
STAPHYLOCOCCUS AUREUS
ZINC OXIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The aim of this work is to evaluate the antibacterial effect and biocompatibility of zinc oxide (ZnO) nanopaticles and graphite-type carbon (Gt) microparticles commercial powders. SEM analysis was performed to assess particles morphology. The antibacterial behavior was studied against Staphylococcus aureus (Staph. Aureus) (bacterial strain ATCC 29213) and TEM analysis of bacteria was performed to determine ultrastructural alterations; in addition, biocompatibility was evaluated in subcutaneous tissue of Wistar rats at 3, 7 and 28 d. ZnO and Gt powders exhibited antibacterial activity while TEM images of Staph. aureus showed membrane disruption followed by the release of internal content. Also, an electron-light region within the cytoplasm was observed for microorganisms treated with ZnO. Regarding biocompatibility, Gt samples induced a foreign body reaction response with presence of giant cells whereas ZnO samples showed fibroblastic connective tissue with chronic inflammatory cells and new small vessels. Also, collagen fibers and lack of capsule was observed by Trichome Masson stain. Thus, ZnO improved wound healing by enhancing tissue regeneration in contrast with calcium hydroxide control sample response which showed a fibrous tissue scar. Hence, ZnO nano-powder seems to be a potential material in the regenerative endodontic field.
Fil: Kozuszko, Silvia Noemi. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; Argentina
Fil: Sanchez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; Argentina
Fil: de Ferro, María Inés Gutiérrez. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Sfer, Ana María. Universidad Nacioal de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Estadística; Argentina
Fil: Moreno Madrid, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; Argentina
Fil: Takabatake, Kiyofumi. Okayama University; Japón
Fil: Nakano, Keisuke. Okayama University; Japón
Fil: Nagatsuka, Hitoshi. Okayama University; Japón
Fil: Rodriguez, Andrea Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería; Argentina
description The aim of this work is to evaluate the antibacterial effect and biocompatibility of zinc oxide (ZnO) nanopaticles and graphite-type carbon (Gt) microparticles commercial powders. SEM analysis was performed to assess particles morphology. The antibacterial behavior was studied against Staphylococcus aureus (Staph. Aureus) (bacterial strain ATCC 29213) and TEM analysis of bacteria was performed to determine ultrastructural alterations; in addition, biocompatibility was evaluated in subcutaneous tissue of Wistar rats at 3, 7 and 28 d. ZnO and Gt powders exhibited antibacterial activity while TEM images of Staph. aureus showed membrane disruption followed by the release of internal content. Also, an electron-light region within the cytoplasm was observed for microorganisms treated with ZnO. Regarding biocompatibility, Gt samples induced a foreign body reaction response with presence of giant cells whereas ZnO samples showed fibroblastic connective tissue with chronic inflammatory cells and new small vessels. Also, collagen fibers and lack of capsule was observed by Trichome Masson stain. Thus, ZnO improved wound healing by enhancing tissue regeneration in contrast with calcium hydroxide control sample response which showed a fibrous tissue scar. Hence, ZnO nano-powder seems to be a potential material in the regenerative endodontic field.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53856
Kozuszko, Silvia Noemi; Sanchez, Maria Alejandra; de Ferro, María Inés Gutiérrez; Sfer, Ana María; Moreno Madrid, Ana Paula; et al.; Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials; The Society for Hard Tissue Regenerative Biology; Journal of Hard Tissue Biology; 26; 4; 10-2017; 311-318
1341-7649
1880-828X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53856
identifier_str_mv Kozuszko, Silvia Noemi; Sanchez, Maria Alejandra; de Ferro, María Inés Gutiérrez; Sfer, Ana María; Moreno Madrid, Ana Paula; et al.; Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials; The Society for Hard Tissue Regenerative Biology; Journal of Hard Tissue Biology; 26; 4; 10-2017; 311-318
1341-7649
1880-828X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2485/jhtb.26.311
info:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/jhtb/26/4/26_311/_article/-char/en
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv The Society for Hard Tissue Regenerative Biology
publisher.none.fl_str_mv The Society for Hard Tissue Regenerative Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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