Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillatio...

Autores
Manso, Victoria; Costantino, Andrea Rosana; Lazzarini, Natalia Anahi; Scarone, Nahuel; Moldes, Carlos Alberto; Bellozas Reinhard, Monica; Minig, Marisol
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Baccharis spartioides is a plant aromatic member of the Asteraceae family. Its branches are strongly scent because of the presence of a wide variety of volatile compounds that have demonstrated antimicrobial activity. The present work aims to determine essential oil composition of B. spartioides (BsEO) and assess the antimicrobial activity against Staphylococcus aureus. BsEO was obtain by steam distillation, and its composition was determine by GC-MS. The antimicrobial activity was evaluated by a disc diffusion test in which the diameter of the clear zone around the disc was measured and expressed in millimeters. It was assess the antimicrobial activity by increasing volumes of BsEO impregnated on disc using 6 L of pure limonene (97%) and Amikasin (30 g) as discs control. It was considered diameters >20 mm by significant inhibitory effect. The majors constituents of BsEO were limonene (44%), sphatulenol (12%) and caryophyllene oxide (8%). Since limonene is BsEO major constituent, we considered this component as responsible for antimicrobial activity. Thus, the calibration curve of L of limonene vs diameter was made and it was determined that 6 L of BsEO was the minimal volume for significant inhibitory effects. Major antimicrobial activity was expressed in discs impregnate with 5, 6, 7, and 8 L of BsEO, which diameters was inferior to 20 mm. Results indicate BsEO present low antimicrobial activity (<20 mm) a but inhibition diameters founded (10-15 mm) could correspond to bacteriostatic activity.
Fil: Manso, Victoria. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Costantino, Andrea Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lazzarini, Natalia Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Scarone, Nahuel. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Gobierno de la Provincia de la Pampa. Hospital Doctor Lucio Molas;
Fil: Moldes, Carlos Alberto. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Bellozas Reinhard, Monica. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Minig, Marisol. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
BACCHARIS SPARTIOIDES
ESSENTIAL OILS
ANTIBACTERIAL
STAPHYLOCOCCUS AUREUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272893
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272893
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillationManso, VictoriaCostantino, Andrea RosanaLazzarini, Natalia AnahiScarone, NahuelMoldes, Carlos AlbertoBellozas Reinhard, MonicaMinig, MarisolBACCHARIS SPARTIOIDESESSENTIAL OILSANTIBACTERIALSTAPHYLOCOCCUS AUREUShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Baccharis spartioides is a plant aromatic member of the Asteraceae family. Its branches are strongly scent because of the presence of a wide variety of volatile compounds that have demonstrated antimicrobial activity. The present work aims to determine essential oil composition of B. spartioides (BsEO) and assess the antimicrobial activity against Staphylococcus aureus. BsEO was obtain by steam distillation, and its composition was determine by GC-MS. The antimicrobial activity was evaluated by a disc diffusion test in which the diameter of the clear zone around the disc was measured and expressed in millimeters. It was assess the antimicrobial activity by increasing volumes of BsEO impregnated on disc using 6 L of pure limonene (97%) and Amikasin (30 g) as discs control. It was considered diameters >20 mm by significant inhibitory effect. The majors constituents of BsEO were limonene (44%), sphatulenol (12%) and caryophyllene oxide (8%). Since limonene is BsEO major constituent, we considered this component as responsible for antimicrobial activity. Thus, the calibration curve of L of limonene vs diameter was made and it was determined that 6 L of BsEO was the minimal volume for significant inhibitory effects. Major antimicrobial activity was expressed in discs impregnate with 5, 6, 7, and 8 L of BsEO, which diameters was inferior to 20 mm. Results indicate BsEO present low antimicrobial activity (<20 mm) a but inhibition diameters founded (10-15 mm) could correspond to bacteriostatic activity.Fil: Manso, Victoria. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Costantino, Andrea Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Lazzarini, Natalia Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Scarone, Nahuel. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Gobierno de la Provincia de la Pampa. Hospital Doctor Lucio Molas;Fil: Moldes, Carlos Alberto. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Bellozas Reinhard, Monica. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Minig, Marisol. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaXXXVII Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272893Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 63-630327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44nSuppl.1/39488Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:20:22Zoai:ri.conicet.gov.ar:11336/272893instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:20:22.45CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation
title Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation
spellingShingle Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation
Manso, Victoria
BACCHARIS SPARTIOIDES
ESSENTIAL OILS
ANTIBACTERIAL
STAPHYLOCOCCUS AUREUS
title_short Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation
title_full Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation
title_fullStr Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation
title_full_unstemmed Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation
title_sort Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation
dc.creator.none.fl_str_mv Manso, Victoria
Costantino, Andrea Rosana
Lazzarini, Natalia Anahi
Scarone, Nahuel
Moldes, Carlos Alberto
Bellozas Reinhard, Monica
Minig, Marisol
author Manso, Victoria
author_facet Manso, Victoria
Costantino, Andrea Rosana
Lazzarini, Natalia Anahi
Scarone, Nahuel
Moldes, Carlos Alberto
Bellozas Reinhard, Monica
Minig, Marisol
author_role author
author2 Costantino, Andrea Rosana
Lazzarini, Natalia Anahi
Scarone, Nahuel
Moldes, Carlos Alberto
Bellozas Reinhard, Monica
Minig, Marisol
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BACCHARIS SPARTIOIDES
ESSENTIAL OILS
ANTIBACTERIAL
STAPHYLOCOCCUS AUREUS
topic BACCHARIS SPARTIOIDES
ESSENTIAL OILS
ANTIBACTERIAL
STAPHYLOCOCCUS AUREUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Baccharis spartioides is a plant aromatic member of the Asteraceae family. Its branches are strongly scent because of the presence of a wide variety of volatile compounds that have demonstrated antimicrobial activity. The present work aims to determine essential oil composition of B. spartioides (BsEO) and assess the antimicrobial activity against Staphylococcus aureus. BsEO was obtain by steam distillation, and its composition was determine by GC-MS. The antimicrobial activity was evaluated by a disc diffusion test in which the diameter of the clear zone around the disc was measured and expressed in millimeters. It was assess the antimicrobial activity by increasing volumes of BsEO impregnated on disc using 6 L of pure limonene (97%) and Amikasin (30 g) as discs control. It was considered diameters >20 mm by significant inhibitory effect. The majors constituents of BsEO were limonene (44%), sphatulenol (12%) and caryophyllene oxide (8%). Since limonene is BsEO major constituent, we considered this component as responsible for antimicrobial activity. Thus, the calibration curve of L of limonene vs diameter was made and it was determined that 6 L of BsEO was the minimal volume for significant inhibitory effects. Major antimicrobial activity was expressed in discs impregnate with 5, 6, 7, and 8 L of BsEO, which diameters was inferior to 20 mm. Results indicate BsEO present low antimicrobial activity (<20 mm) a but inhibition diameters founded (10-15 mm) could correspond to bacteriostatic activity.
Fil: Manso, Victoria. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Costantino, Andrea Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lazzarini, Natalia Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Scarone, Nahuel. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Gobierno de la Provincia de la Pampa. Hospital Doctor Lucio Molas;
Fil: Moldes, Carlos Alberto. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Bellozas Reinhard, Monica. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Minig, Marisol. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description Baccharis spartioides is a plant aromatic member of the Asteraceae family. Its branches are strongly scent because of the presence of a wide variety of volatile compounds that have demonstrated antimicrobial activity. The present work aims to determine essential oil composition of B. spartioides (BsEO) and assess the antimicrobial activity against Staphylococcus aureus. BsEO was obtain by steam distillation, and its composition was determine by GC-MS. The antimicrobial activity was evaluated by a disc diffusion test in which the diameter of the clear zone around the disc was measured and expressed in millimeters. It was assess the antimicrobial activity by increasing volumes of BsEO impregnated on disc using 6 L of pure limonene (97%) and Amikasin (30 g) as discs control. It was considered diameters >20 mm by significant inhibitory effect. The majors constituents of BsEO were limonene (44%), sphatulenol (12%) and caryophyllene oxide (8%). Since limonene is BsEO major constituent, we considered this component as responsible for antimicrobial activity. Thus, the calibration curve of L of limonene vs diameter was made and it was determined that 6 L of BsEO was the minimal volume for significant inhibitory effects. Major antimicrobial activity was expressed in discs impregnate with 5, 6, 7, and 8 L of BsEO, which diameters was inferior to 20 mm. Results indicate BsEO present low antimicrobial activity (<20 mm) a but inhibition diameters founded (10-15 mm) could correspond to bacteriostatic activity.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272893
Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 63-63
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272893
identifier_str_mv Chemical composition and antibacterial activity against staphylococcus aureus ATCC 25923 of the essential oils from Baccharis spartioides ("Pichana") extracted by steam distillation; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 63-63
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44nSuppl.1/39488
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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