Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed

Autores
Abeyá, María Mercedes; Metz, German Ernesto; Franco Cruz, Rodrigo; Correas, Ignacio; Osorio, Fernando Abel; Echeverria, Maria Gabriela
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Equine arteritis virus infection in horse populations could be confirmed by the OIE recommended Virus Neutralization (VN) test and by the gold standard Virus Isolation (VI). These two techniques involve the observation of the cytopathic effect (CPE) of EAV. The characteristic CPE in EAV infections is the cellular lysis. The presence/ absence of this CPE in cells in the VI/VN respectively, indicate the positivity of each test. CPE refers to morphological and molecular changes that where evidence in infect cells after viral infections. Most viral infections eventually result in the death of the host cell by different cellular mechanisms. The causes of death include cell lysis by alterations to the cell’s surface membrane and by various modes of programmed cell death such as necrosis, apoptosis, autophagy and others. EAV CPE is always refers as cellular lysis but the mechanism involve in this lytical effect has never specifically determined. So, our objective is to extend the concept of the cytopathic effect of EAV infections. To study the effect of different cell death mechanism in EAV CPE we used different inhibitors. Consequently, we concluded that the most important mechanism of cell death in EAV infections is caspase-dependent cell death.
Fil: Abeyá, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Metz, German Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Franco Cruz, Rodrigo. University of Nebraska; Estados Unidos
Fil: Correas, Ignacio. University of Nebraska; Estados Unidos
Fil: Osorio, Fernando Abel. University of Nebraska; Estados Unidos
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Materia
EQUINE ARTERITIS VIRUS
CYTOPATHIC EFFECT
CASPASE-DEPENDENT CELL DEATH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133733
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observedAbeyá, María MercedesMetz, German ErnestoFranco Cruz, RodrigoCorreas, IgnacioOsorio, Fernando AbelEcheverria, Maria GabrielaEQUINE ARTERITIS VIRUSCYTOPATHIC EFFECTCASPASE-DEPENDENT CELL DEATHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Equine arteritis virus infection in horse populations could be confirmed by the OIE recommended Virus Neutralization (VN) test and by the gold standard Virus Isolation (VI). These two techniques involve the observation of the cytopathic effect (CPE) of EAV. The characteristic CPE in EAV infections is the cellular lysis. The presence/ absence of this CPE in cells in the VI/VN respectively, indicate the positivity of each test. CPE refers to morphological and molecular changes that where evidence in infect cells after viral infections. Most viral infections eventually result in the death of the host cell by different cellular mechanisms. The causes of death include cell lysis by alterations to the cell’s surface membrane and by various modes of programmed cell death such as necrosis, apoptosis, autophagy and others. EAV CPE is always refers as cellular lysis but the mechanism involve in this lytical effect has never specifically determined. So, our objective is to extend the concept of the cytopathic effect of EAV infections. To study the effect of different cell death mechanism in EAV CPE we used different inhibitors. Consequently, we concluded that the most important mechanism of cell death in EAV infections is caspase-dependent cell death.Fil: Abeyá, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Metz, German Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Franco Cruz, Rodrigo. University of Nebraska; Estados UnidosFil: Correas, Ignacio. University of Nebraska; Estados UnidosFil: Osorio, Fernando Abel. University of Nebraska; Estados UnidosFil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaMedCrave2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133733Abeyá, María Mercedes; Metz, German Ernesto; Franco Cruz, Rodrigo; Correas, Ignacio; Osorio, Fernando Abel; et al.; Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed; MedCrave; Journal of Microbiology & Experimentation; 6; 4; 7-2018; 171-1732373-437XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medcraveonline.com/JMEN/equine-arteritis-virus-cytopathic-effect-caspase-dependent-cell-death-as-the-major-consequence-observed.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.15406/jmen.2018.06.00210info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:09:29Zoai:ri.conicet.gov.ar:11336/133733instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:09:29.822CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed
title Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed
spellingShingle Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed
Abeyá, María Mercedes
EQUINE ARTERITIS VIRUS
CYTOPATHIC EFFECT
CASPASE-DEPENDENT CELL DEATH
title_short Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed
title_full Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed
title_fullStr Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed
title_full_unstemmed Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed
title_sort Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed
dc.creator.none.fl_str_mv Abeyá, María Mercedes
Metz, German Ernesto
Franco Cruz, Rodrigo
Correas, Ignacio
Osorio, Fernando Abel
Echeverria, Maria Gabriela
author Abeyá, María Mercedes
author_facet Abeyá, María Mercedes
Metz, German Ernesto
Franco Cruz, Rodrigo
Correas, Ignacio
Osorio, Fernando Abel
Echeverria, Maria Gabriela
author_role author
author2 Metz, German Ernesto
Franco Cruz, Rodrigo
Correas, Ignacio
Osorio, Fernando Abel
Echeverria, Maria Gabriela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv EQUINE ARTERITIS VIRUS
CYTOPATHIC EFFECT
CASPASE-DEPENDENT CELL DEATH
topic EQUINE ARTERITIS VIRUS
CYTOPATHIC EFFECT
CASPASE-DEPENDENT CELL DEATH
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Equine arteritis virus infection in horse populations could be confirmed by the OIE recommended Virus Neutralization (VN) test and by the gold standard Virus Isolation (VI). These two techniques involve the observation of the cytopathic effect (CPE) of EAV. The characteristic CPE in EAV infections is the cellular lysis. The presence/ absence of this CPE in cells in the VI/VN respectively, indicate the positivity of each test. CPE refers to morphological and molecular changes that where evidence in infect cells after viral infections. Most viral infections eventually result in the death of the host cell by different cellular mechanisms. The causes of death include cell lysis by alterations to the cell’s surface membrane and by various modes of programmed cell death such as necrosis, apoptosis, autophagy and others. EAV CPE is always refers as cellular lysis but the mechanism involve in this lytical effect has never specifically determined. So, our objective is to extend the concept of the cytopathic effect of EAV infections. To study the effect of different cell death mechanism in EAV CPE we used different inhibitors. Consequently, we concluded that the most important mechanism of cell death in EAV infections is caspase-dependent cell death.
Fil: Abeyá, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Metz, German Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Franco Cruz, Rodrigo. University of Nebraska; Estados Unidos
Fil: Correas, Ignacio. University of Nebraska; Estados Unidos
Fil: Osorio, Fernando Abel. University of Nebraska; Estados Unidos
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
description Equine arteritis virus infection in horse populations could be confirmed by the OIE recommended Virus Neutralization (VN) test and by the gold standard Virus Isolation (VI). These two techniques involve the observation of the cytopathic effect (CPE) of EAV. The characteristic CPE in EAV infections is the cellular lysis. The presence/ absence of this CPE in cells in the VI/VN respectively, indicate the positivity of each test. CPE refers to morphological and molecular changes that where evidence in infect cells after viral infections. Most viral infections eventually result in the death of the host cell by different cellular mechanisms. The causes of death include cell lysis by alterations to the cell’s surface membrane and by various modes of programmed cell death such as necrosis, apoptosis, autophagy and others. EAV CPE is always refers as cellular lysis but the mechanism involve in this lytical effect has never specifically determined. So, our objective is to extend the concept of the cytopathic effect of EAV infections. To study the effect of different cell death mechanism in EAV CPE we used different inhibitors. Consequently, we concluded that the most important mechanism of cell death in EAV infections is caspase-dependent cell death.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133733
Abeyá, María Mercedes; Metz, German Ernesto; Franco Cruz, Rodrigo; Correas, Ignacio; Osorio, Fernando Abel; et al.; Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed; MedCrave; Journal of Microbiology & Experimentation; 6; 4; 7-2018; 171-173
2373-437X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133733
identifier_str_mv Abeyá, María Mercedes; Metz, German Ernesto; Franco Cruz, Rodrigo; Correas, Ignacio; Osorio, Fernando Abel; et al.; Equine arteritis virus cytopathic effect: caspase-dependent cell death as the major consequence observed; MedCrave; Journal of Microbiology & Experimentation; 6; 4; 7-2018; 171-173
2373-437X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medcraveonline.com/JMEN/equine-arteritis-virus-cytopathic-effect-caspase-dependent-cell-death-as-the-major-consequence-observed.html
info:eu-repo/semantics/altIdentifier/doi/10.15406/jmen.2018.06.00210
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MedCrave
publisher.none.fl_str_mv MedCrave
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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