Development of a peptide ELISA for the diagnosis of Equine arteritis virus

Autores
Metz, German Ernesto; Lorenzon, Esteban Nicolás; Serena, Maria Soledad; Corva, Santiago Gerardo; Panei, Carlos Javier; Diaz, Silvina; Maffud Cilli, Eduardo; Echeverria, Maria Gabriela
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations.
Fil: Metz, German Ernesto. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lorenzon, Esteban Nicolás. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Serena, Maria Soledad. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Corva, Santiago Gerardo. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Epidemiología; Argentina
Fil: Panei, Carlos Javier. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diaz, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina
Fil: Maffud Cilli, Eduardo. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Echeverria, Maria Gabriela. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina
Materia
SYNTHETIC PEPTIDES
ELISA
EQUINE ARTERITIS VIRUS
DIAGNOSTIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11505
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11505
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a peptide ELISA for the diagnosis of Equine arteritis virusMetz, German ErnestoLorenzon, Esteban NicolásSerena, Maria SoledadCorva, Santiago GerardoPanei, Carlos JavierDiaz, SilvinaMaffud Cilli, EduardoEcheverria, Maria GabrielaSYNTHETIC PEPTIDESELISAEQUINE ARTERITIS VIRUSDIAGNOSTIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations.Fil: Metz, German Ernesto. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lorenzon, Esteban Nicolás. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Serena, Maria Soledad. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Corva, Santiago Gerardo. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Epidemiología; ArgentinaFil: Panei, Carlos Javier. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diaz, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; ArgentinaFil: Maffud Cilli, Eduardo. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Echeverria, Maria Gabriela. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; ArgentinaElsevier Science2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11505Metz, German Ernesto; Lorenzon, Esteban Nicolás; Serena, Maria Soledad; Corva, Santiago Gerardo; Panei, Carlos Javier; et al.; Development of a peptide ELISA for the diagnosis of Equine arteritis virus; Elsevier Science; Journal Of Virological Methods; 205; 9-2014; 3-60166-0934enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2014.04.018info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093414001785info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:25:27Zoai:ri.conicet.gov.ar:11336/11505instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:25:27.767CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a peptide ELISA for the diagnosis of Equine arteritis virus
title Development of a peptide ELISA for the diagnosis of Equine arteritis virus
spellingShingle Development of a peptide ELISA for the diagnosis of Equine arteritis virus
Metz, German Ernesto
SYNTHETIC PEPTIDES
ELISA
EQUINE ARTERITIS VIRUS
DIAGNOSTIC
title_short Development of a peptide ELISA for the diagnosis of Equine arteritis virus
title_full Development of a peptide ELISA for the diagnosis of Equine arteritis virus
title_fullStr Development of a peptide ELISA for the diagnosis of Equine arteritis virus
title_full_unstemmed Development of a peptide ELISA for the diagnosis of Equine arteritis virus
title_sort Development of a peptide ELISA for the diagnosis of Equine arteritis virus
dc.creator.none.fl_str_mv Metz, German Ernesto
Lorenzon, Esteban Nicolás
Serena, Maria Soledad
Corva, Santiago Gerardo
Panei, Carlos Javier
Diaz, Silvina
Maffud Cilli, Eduardo
Echeverria, Maria Gabriela
author Metz, German Ernesto
author_facet Metz, German Ernesto
Lorenzon, Esteban Nicolás
Serena, Maria Soledad
Corva, Santiago Gerardo
Panei, Carlos Javier
Diaz, Silvina
Maffud Cilli, Eduardo
Echeverria, Maria Gabriela
author_role author
author2 Lorenzon, Esteban Nicolás
Serena, Maria Soledad
Corva, Santiago Gerardo
Panei, Carlos Javier
Diaz, Silvina
Maffud Cilli, Eduardo
Echeverria, Maria Gabriela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SYNTHETIC PEPTIDES
ELISA
EQUINE ARTERITIS VIRUS
DIAGNOSTIC
topic SYNTHETIC PEPTIDES
ELISA
EQUINE ARTERITIS VIRUS
DIAGNOSTIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations.
Fil: Metz, German Ernesto. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lorenzon, Esteban Nicolás. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Serena, Maria Soledad. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Corva, Santiago Gerardo. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Epidemiología; Argentina
Fil: Panei, Carlos Javier. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diaz, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina
Fil: Maffud Cilli, Eduardo. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Echeverria, Maria Gabriela. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina
description A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11505
Metz, German Ernesto; Lorenzon, Esteban Nicolás; Serena, Maria Soledad; Corva, Santiago Gerardo; Panei, Carlos Javier; et al.; Development of a peptide ELISA for the diagnosis of Equine arteritis virus; Elsevier Science; Journal Of Virological Methods; 205; 9-2014; 3-6
0166-0934
url http://hdl.handle.net/11336/11505
identifier_str_mv Metz, German Ernesto; Lorenzon, Esteban Nicolás; Serena, Maria Soledad; Corva, Santiago Gerardo; Panei, Carlos Javier; et al.; Development of a peptide ELISA for the diagnosis of Equine arteritis virus; Elsevier Science; Journal Of Virological Methods; 205; 9-2014; 3-6
0166-0934
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2014.04.018
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093414001785
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981410536685568
score 12.993085

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