Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients

Autores
Tateosian, Nancy Liliana; Costa, Maria Julieta; Guerrieri, Diego; Barro, Analía; Mazzei, Juan A.; Chuluyan, Hector Eduardo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Samples of exhaled breath condensate (EBC) provide a convenient and non-invasive method to study inflammation in lung diseases. The aim of the present study was to evaluate and compare the inflammatory protein mediator levels in EBC from healthy donors (HD) and from patients with exacerbation of chronic obstructive pulmonary disease (COPD) using an EBC collection device with and without a coating of albumin as a carrier. We studied 13 HD and 26 patients with exacerbation of COPD. The concentrations of myeloperoxidase (MPO), IFNγ and secretory leukocyte protease inhibitor (SLPI) in EBC were measured by immunoassays. The EBC samples from HD and COPD patients showed higher concentrations of MPO when samples were recovered with an albumin-coated device. Furthermore, levels of MPO in COPD patients were significantly higher than in HD. An inverse correlation was observed between MPO and spirometric parameters (FVC and FEV1). Almost all samples collected with the albumin-coated device showed higher amounts of IFNγ and SLPI than those collected with the uncoated device. The levels of SLPI in COPD patients were significantly higher than in HD. A direct correlation was observed between FVC% predicted and SLPI. We concluded that coating the collection device with albumin increased the sensitivity of the technique, at least for measurements of MPO, SLPI and IFNγ. Furthermore, the higher levels of MPO and SLPI and lower levels of IFNγ in EBC from COPD patients could reflect the immunological status and the response of lung parenchyma to treatment during the exacerbation of the illness.
Fil: Tateosian, Nancy Liliana. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Costa, Maria Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina
Fil: Guerrieri, Diego. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barro, Analía. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Mazzei, Juan A.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Chuluyan, Hector Eduardo. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Copd
Slpi
Exhaled Breath Condensate
Mpo
Ifnγ
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17233
Acceder
id CONICETDig_8010ffecba040faf3b38db417a850177
oai_identifier_str oai:ri.conicet.gov.ar:11336/17233
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patientsTateosian, Nancy LilianaCosta, Maria JulietaGuerrieri, DiegoBarro, AnalíaMazzei, Juan A.Chuluyan, Hector EduardoCopdSlpiExhaled Breath CondensateMpoIfnγhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Samples of exhaled breath condensate (EBC) provide a convenient and non-invasive method to study inflammation in lung diseases. The aim of the present study was to evaluate and compare the inflammatory protein mediator levels in EBC from healthy donors (HD) and from patients with exacerbation of chronic obstructive pulmonary disease (COPD) using an EBC collection device with and without a coating of albumin as a carrier. We studied 13 HD and 26 patients with exacerbation of COPD. The concentrations of myeloperoxidase (MPO), IFNγ and secretory leukocyte protease inhibitor (SLPI) in EBC were measured by immunoassays. The EBC samples from HD and COPD patients showed higher concentrations of MPO when samples were recovered with an albumin-coated device. Furthermore, levels of MPO in COPD patients were significantly higher than in HD. An inverse correlation was observed between MPO and spirometric parameters (FVC and FEV1). Almost all samples collected with the albumin-coated device showed higher amounts of IFNγ and SLPI than those collected with the uncoated device. The levels of SLPI in COPD patients were significantly higher than in HD. A direct correlation was observed between FVC% predicted and SLPI. We concluded that coating the collection device with albumin increased the sensitivity of the technique, at least for measurements of MPO, SLPI and IFNγ. Furthermore, the higher levels of MPO and SLPI and lower levels of IFNγ in EBC from COPD patients could reflect the immunological status and the response of lung parenchyma to treatment during the exacerbation of the illness.Fil: Tateosian, Nancy Liliana. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Costa, Maria Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; ArgentinaFil: Guerrieri, Diego. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barro, Analía. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Mazzei, Juan A.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Chuluyan, Hector Eduardo. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17233Tateosian, Nancy Liliana; Costa, Maria Julieta; Guerrieri, Diego; Barro, Analía; Mazzei, Juan A.; et al.; Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients; Elsevier; Cytokine.; 58; 3; 6-2012; 361-3671043-4666enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cyto.2012.03.006info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1043466612000890info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:46Zoai:ri.conicet.gov.ar:11336/17233instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:47.072CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients
title Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients
spellingShingle Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients
Tateosian, Nancy Liliana
Copd
Slpi
Exhaled Breath Condensate
Mpo
Ifnγ
title_short Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients
title_full Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients
title_fullStr Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients
title_full_unstemmed Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients
title_sort Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients
dc.creator.none.fl_str_mv Tateosian, Nancy Liliana
Costa, Maria Julieta
Guerrieri, Diego
Barro, Analía
Mazzei, Juan A.
Chuluyan, Hector Eduardo
author Tateosian, Nancy Liliana
author_facet Tateosian, Nancy Liliana
Costa, Maria Julieta
Guerrieri, Diego
Barro, Analía
Mazzei, Juan A.
Chuluyan, Hector Eduardo
author_role author
author2 Costa, Maria Julieta
Guerrieri, Diego
Barro, Analía
Mazzei, Juan A.
Chuluyan, Hector Eduardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Copd
Slpi
Exhaled Breath Condensate
Mpo
Ifnγ
topic Copd
Slpi
Exhaled Breath Condensate
Mpo
Ifnγ
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Samples of exhaled breath condensate (EBC) provide a convenient and non-invasive method to study inflammation in lung diseases. The aim of the present study was to evaluate and compare the inflammatory protein mediator levels in EBC from healthy donors (HD) and from patients with exacerbation of chronic obstructive pulmonary disease (COPD) using an EBC collection device with and without a coating of albumin as a carrier. We studied 13 HD and 26 patients with exacerbation of COPD. The concentrations of myeloperoxidase (MPO), IFNγ and secretory leukocyte protease inhibitor (SLPI) in EBC were measured by immunoassays. The EBC samples from HD and COPD patients showed higher concentrations of MPO when samples were recovered with an albumin-coated device. Furthermore, levels of MPO in COPD patients were significantly higher than in HD. An inverse correlation was observed between MPO and spirometric parameters (FVC and FEV1). Almost all samples collected with the albumin-coated device showed higher amounts of IFNγ and SLPI than those collected with the uncoated device. The levels of SLPI in COPD patients were significantly higher than in HD. A direct correlation was observed between FVC% predicted and SLPI. We concluded that coating the collection device with albumin increased the sensitivity of the technique, at least for measurements of MPO, SLPI and IFNγ. Furthermore, the higher levels of MPO and SLPI and lower levels of IFNγ in EBC from COPD patients could reflect the immunological status and the response of lung parenchyma to treatment during the exacerbation of the illness.
Fil: Tateosian, Nancy Liliana. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Costa, Maria Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina
Fil: Guerrieri, Diego. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barro, Analía. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Mazzei, Juan A.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Chuluyan, Hector Eduardo. UNIVERSIDAD DE BUENOS AIRES. FACULTAD DE MEDICINA. CÁTEDRA DE FARMACOLOGÍA. 3º CÁTEDRA DE FARMACOLOGÍA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Samples of exhaled breath condensate (EBC) provide a convenient and non-invasive method to study inflammation in lung diseases. The aim of the present study was to evaluate and compare the inflammatory protein mediator levels in EBC from healthy donors (HD) and from patients with exacerbation of chronic obstructive pulmonary disease (COPD) using an EBC collection device with and without a coating of albumin as a carrier. We studied 13 HD and 26 patients with exacerbation of COPD. The concentrations of myeloperoxidase (MPO), IFNγ and secretory leukocyte protease inhibitor (SLPI) in EBC were measured by immunoassays. The EBC samples from HD and COPD patients showed higher concentrations of MPO when samples were recovered with an albumin-coated device. Furthermore, levels of MPO in COPD patients were significantly higher than in HD. An inverse correlation was observed between MPO and spirometric parameters (FVC and FEV1). Almost all samples collected with the albumin-coated device showed higher amounts of IFNγ and SLPI than those collected with the uncoated device. The levels of SLPI in COPD patients were significantly higher than in HD. A direct correlation was observed between FVC% predicted and SLPI. We concluded that coating the collection device with albumin increased the sensitivity of the technique, at least for measurements of MPO, SLPI and IFNγ. Furthermore, the higher levels of MPO and SLPI and lower levels of IFNγ in EBC from COPD patients could reflect the immunological status and the response of lung parenchyma to treatment during the exacerbation of the illness.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17233
Tateosian, Nancy Liliana; Costa, Maria Julieta; Guerrieri, Diego; Barro, Analía; Mazzei, Juan A.; et al.; Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients; Elsevier; Cytokine.; 58; 3; 6-2012; 361-367
1043-4666
url http://hdl.handle.net/11336/17233
identifier_str_mv Tateosian, Nancy Liliana; Costa, Maria Julieta; Guerrieri, Diego; Barro, Analía; Mazzei, Juan A.; et al.; Inflammatory mediators in exhaled breath condensate of healthy donors and exacerbated COPD patients; Elsevier; Cytokine.; 58; 3; 6-2012; 361-367
1043-4666
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cyto.2012.03.006
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1043466612000890
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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