The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity

Autores
Tateosian, Nancy Liliana; Pasquinelli, Virginia; Hernández Del Pino, Rodrigo Emanuel; Ambrosi, Nella Gabriela; Guerrieri, Diego; Pedraza Sánchez, Sigifredo; Santucci, Natalia Estefanía; D'attilio, Luciano David; Pellegrini, Joaquín Miguel; Araujo Solis, María A.; Musella, Rosa M.; Palmero, Domingo J.; Hernandez Pando, Rogelio; Garcia, Veronica Edith; Chuluyan, Hector Eduardo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Interferon (IFN)-g displays a critical role in tuberculosis (TB), modulating the innate and adaptive immune responses. Previously, we reported that secretory leukocyte protease inhibitor (SLPI) is a pattern recognition receptor with anti-mycobacterial activity against Mycobacterium tuberculosis (Mtb). Herein, we determined whether IFN-g modulated the levels of SLPI in TB patients. Plasma levels of SLPI and IFN-g were studied in healthy donors (HDs) and TB patients. Peripheral blood mononuclear cells from HDs and patients with TB or defective IFN-g receptor 1* were stimulated with Mtb antigen and SLPI, and IFN-gR expression levels were measured. Both SLPI and IFN-g were significantly enhanced in plasma from those with TB compared with HDs. A direct association between SLPI levels and the severity of TB was detected. In addition, Mtb antigen stimulation decreased the SLPI produced by peripheral blood mononuclear cells from HDs, but not from TB or IFN-gR patients. Neutralization of IFN-g reversed the inhibition of SLPI induced by Mtb antigen in HDs, but not in TB patients. Furthermore, recombinant IFN-g was unable to modify the expression of SLPI in TB patients. Finally, IFN-gR expression was lower in TB compared with HD peripheral blood mononuclear cells. These results show that Mtb-induced IFN-g down-modulated SLPI levels by signaling through the IFNgR in HDs. This inhibitory mechanism was not observed in TB, probably because of the low expression of IFN-gR detected in these individuals. (Am J Pathol 2014, 184: 1e6
Fil: Tateosian, Nancy Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Argentina
Fil: Pasquinelli, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Hernández Del Pino, Rodrigo Emanuel. Universidad de Buenos Aires; Argentina
Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Argentina
Fil: Pedraza Sánchez, Sigifredo. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: Santucci, Natalia Estefanía. Universidad Nacional de Rosario; Argentina
Fil: D'attilio, Luciano David. Universidad Nacional de Rosario; Argentina
Fil: Pellegrini, Joaquín Miguel. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Araujo Solis, María A.. Instituto Mexicano del Seguro Social; México
Fil: Musella, Rosa M.. Hospital "F. J. Muñiz"; Argentina
Fil: Palmero, Domingo J.. Hospital "F. J. Muñiz"; Argentina
Fil: Hernandez Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: Garcia, Veronica Edith. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Argentina
Materia
TUBERCULOSIS
IFN-g
SLPI
RECEPTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15280
Acceder
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spelling The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease SeverityTateosian, Nancy LilianaPasquinelli, VirginiaHernández Del Pino, Rodrigo EmanuelAmbrosi, Nella GabrielaGuerrieri, DiegoPedraza Sánchez, SigifredoSantucci, Natalia EstefaníaD'attilio, Luciano DavidPellegrini, Joaquín MiguelAraujo Solis, María A.Musella, Rosa M.Palmero, Domingo J.Hernandez Pando, RogelioGarcia, Veronica EdithChuluyan, Hector EduardoTUBERCULOSISIFN-gSLPIRECEPTORhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Interferon (IFN)-g displays a critical role in tuberculosis (TB), modulating the innate and adaptive immune responses. Previously, we reported that secretory leukocyte protease inhibitor (SLPI) is a pattern recognition receptor with anti-mycobacterial activity against Mycobacterium tuberculosis (Mtb). Herein, we determined whether IFN-g modulated the levels of SLPI in TB patients. Plasma levels of SLPI and IFN-g were studied in healthy donors (HDs) and TB patients. Peripheral blood mononuclear cells from HDs and patients with TB or defective IFN-g receptor 1* were stimulated with Mtb antigen and SLPI, and IFN-gR expression levels were measured. Both SLPI and IFN-g were significantly enhanced in plasma from those with TB compared with HDs. A direct association between SLPI levels and the severity of TB was detected. In addition, Mtb antigen stimulation decreased the SLPI produced by peripheral blood mononuclear cells from HDs, but not from TB or IFN-gR patients. Neutralization of IFN-g reversed the inhibition of SLPI induced by Mtb antigen in HDs, but not in TB patients. Furthermore, recombinant IFN-g was unable to modify the expression of SLPI in TB patients. Finally, IFN-gR expression was lower in TB compared with HD peripheral blood mononuclear cells. These results show that Mtb-induced IFN-g down-modulated SLPI levels by signaling through the IFNgR in HDs. This inhibitory mechanism was not observed in TB, probably because of the low expression of IFN-gR detected in these individuals. (Am J Pathol 2014, 184: 1e6Fil: Tateosian, Nancy Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; ArgentinaFil: Pasquinelli, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Hernández Del Pino, Rodrigo Emanuel. Universidad de Buenos Aires; ArgentinaFil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; ArgentinaFil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; ArgentinaFil: Pedraza Sánchez, Sigifredo. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; MéxicoFil: Santucci, Natalia Estefanía. Universidad Nacional de Rosario; ArgentinaFil: D'attilio, Luciano David. Universidad Nacional de Rosario; ArgentinaFil: Pellegrini, Joaquín Miguel. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Araujo Solis, María A.. Instituto Mexicano del Seguro Social; MéxicoFil: Musella, Rosa M.. Hospital "F. J. Muñiz"; ArgentinaFil: Palmero, Domingo J.. Hospital "F. J. Muñiz"; ArgentinaFil: Hernandez Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; MéxicoFil: Garcia, Veronica Edith. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; ArgentinaAmer Soc Investigative Pathology, Inc2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15280Tateosian, Nancy Liliana; Pasquinelli, Virginia; Hernández Del Pino, Rodrigo Emanuel; Ambrosi, Nella Gabriela; Guerrieri, Diego; et al.; The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity; Amer Soc Investigative Pathology, Inc; American Journal Of Pathology; 184; 5; 5-2014; 1268–12730002-9440enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0002944014000765info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ajpath.2014.01.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:00Zoai:ri.conicet.gov.ar:11336/15280instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:01.249CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity
title The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity
spellingShingle The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity
Tateosian, Nancy Liliana
TUBERCULOSIS
IFN-g
SLPI
RECEPTOR
title_short The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity
title_full The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity
title_fullStr The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity
title_full_unstemmed The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity
title_sort The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity
dc.creator.none.fl_str_mv Tateosian, Nancy Liliana
Pasquinelli, Virginia
Hernández Del Pino, Rodrigo Emanuel
Ambrosi, Nella Gabriela
Guerrieri, Diego
Pedraza Sánchez, Sigifredo
Santucci, Natalia Estefanía
D'attilio, Luciano David
Pellegrini, Joaquín Miguel
Araujo Solis, María A.
Musella, Rosa M.
Palmero, Domingo J.
Hernandez Pando, Rogelio
Garcia, Veronica Edith
Chuluyan, Hector Eduardo
author Tateosian, Nancy Liliana
author_facet Tateosian, Nancy Liliana
Pasquinelli, Virginia
Hernández Del Pino, Rodrigo Emanuel
Ambrosi, Nella Gabriela
Guerrieri, Diego
Pedraza Sánchez, Sigifredo
Santucci, Natalia Estefanía
D'attilio, Luciano David
Pellegrini, Joaquín Miguel
Araujo Solis, María A.
Musella, Rosa M.
Palmero, Domingo J.
Hernandez Pando, Rogelio
Garcia, Veronica Edith
Chuluyan, Hector Eduardo
author_role author
author2 Pasquinelli, Virginia
Hernández Del Pino, Rodrigo Emanuel
Ambrosi, Nella Gabriela
Guerrieri, Diego
Pedraza Sánchez, Sigifredo
Santucci, Natalia Estefanía
D'attilio, Luciano David
Pellegrini, Joaquín Miguel
Araujo Solis, María A.
Musella, Rosa M.
Palmero, Domingo J.
Hernandez Pando, Rogelio
Garcia, Veronica Edith
Chuluyan, Hector Eduardo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TUBERCULOSIS
IFN-g
SLPI
RECEPTOR
topic TUBERCULOSIS
IFN-g
SLPI
RECEPTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Interferon (IFN)-g displays a critical role in tuberculosis (TB), modulating the innate and adaptive immune responses. Previously, we reported that secretory leukocyte protease inhibitor (SLPI) is a pattern recognition receptor with anti-mycobacterial activity against Mycobacterium tuberculosis (Mtb). Herein, we determined whether IFN-g modulated the levels of SLPI in TB patients. Plasma levels of SLPI and IFN-g were studied in healthy donors (HDs) and TB patients. Peripheral blood mononuclear cells from HDs and patients with TB or defective IFN-g receptor 1* were stimulated with Mtb antigen and SLPI, and IFN-gR expression levels were measured. Both SLPI and IFN-g were significantly enhanced in plasma from those with TB compared with HDs. A direct association between SLPI levels and the severity of TB was detected. In addition, Mtb antigen stimulation decreased the SLPI produced by peripheral blood mononuclear cells from HDs, but not from TB or IFN-gR patients. Neutralization of IFN-g reversed the inhibition of SLPI induced by Mtb antigen in HDs, but not in TB patients. Furthermore, recombinant IFN-g was unable to modify the expression of SLPI in TB patients. Finally, IFN-gR expression was lower in TB compared with HD peripheral blood mononuclear cells. These results show that Mtb-induced IFN-g down-modulated SLPI levels by signaling through the IFNgR in HDs. This inhibitory mechanism was not observed in TB, probably because of the low expression of IFN-gR detected in these individuals. (Am J Pathol 2014, 184: 1e6
Fil: Tateosian, Nancy Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Argentina
Fil: Pasquinelli, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Hernández Del Pino, Rodrigo Emanuel. Universidad de Buenos Aires; Argentina
Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Argentina
Fil: Pedraza Sánchez, Sigifredo. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: Santucci, Natalia Estefanía. Universidad Nacional de Rosario; Argentina
Fil: D'attilio, Luciano David. Universidad Nacional de Rosario; Argentina
Fil: Pellegrini, Joaquín Miguel. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Araujo Solis, María A.. Instituto Mexicano del Seguro Social; México
Fil: Musella, Rosa M.. Hospital "F. J. Muñiz"; Argentina
Fil: Palmero, Domingo J.. Hospital "F. J. Muñiz"; Argentina
Fil: Hernandez Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: Garcia, Veronica Edith. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Argentina
description Interferon (IFN)-g displays a critical role in tuberculosis (TB), modulating the innate and adaptive immune responses. Previously, we reported that secretory leukocyte protease inhibitor (SLPI) is a pattern recognition receptor with anti-mycobacterial activity against Mycobacterium tuberculosis (Mtb). Herein, we determined whether IFN-g modulated the levels of SLPI in TB patients. Plasma levels of SLPI and IFN-g were studied in healthy donors (HDs) and TB patients. Peripheral blood mononuclear cells from HDs and patients with TB or defective IFN-g receptor 1* were stimulated with Mtb antigen and SLPI, and IFN-gR expression levels were measured. Both SLPI and IFN-g were significantly enhanced in plasma from those with TB compared with HDs. A direct association between SLPI levels and the severity of TB was detected. In addition, Mtb antigen stimulation decreased the SLPI produced by peripheral blood mononuclear cells from HDs, but not from TB or IFN-gR patients. Neutralization of IFN-g reversed the inhibition of SLPI induced by Mtb antigen in HDs, but not in TB patients. Furthermore, recombinant IFN-g was unable to modify the expression of SLPI in TB patients. Finally, IFN-gR expression was lower in TB compared with HD peripheral blood mononuclear cells. These results show that Mtb-induced IFN-g down-modulated SLPI levels by signaling through the IFNgR in HDs. This inhibitory mechanism was not observed in TB, probably because of the low expression of IFN-gR detected in these individuals. (Am J Pathol 2014, 184: 1e6
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15280
Tateosian, Nancy Liliana; Pasquinelli, Virginia; Hernández Del Pino, Rodrigo Emanuel; Ambrosi, Nella Gabriela; Guerrieri, Diego; et al.; The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity; Amer Soc Investigative Pathology, Inc; American Journal Of Pathology; 184; 5; 5-2014; 1268–1273
0002-9440
url http://hdl.handle.net/11336/15280
identifier_str_mv Tateosian, Nancy Liliana; Pasquinelli, Virginia; Hernández Del Pino, Rodrigo Emanuel; Ambrosi, Nella Gabriela; Guerrieri, Diego; et al.; The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity; Amer Soc Investigative Pathology, Inc; American Journal Of Pathology; 184; 5; 5-2014; 1268–1273
0002-9440
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0002944014000765
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ajpath.2014.01.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
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dc.publisher.none.fl_str_mv Amer Soc Investigative Pathology, Inc
publisher.none.fl_str_mv Amer Soc Investigative Pathology, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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