A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples

Autores
Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new, simple and highly sensitive method for spectrofluorimetric determination of amiloride (AMI) and furosemide (FUR) in pharmaceuticals is presented. The proposed method is based on the separation of AMI from FUR by solid-phase extraction using a nylon membrane, followed by spectrofluorimetric determination of both drugs, on the solid surface and the filtered aqueous solution, respectively. AMI shows low native fluorescence, but its separation-preconcentration by immobilization (solid-phase extraction) on nylon membrane surface provides a considerable enhancement in fluorescence intensity. The fluorescence determination is carried out at λex = 237, λem = 415 nm for FUR; and λex = 365, λem = 406 nm for AMI. The calibration graphs are linear in the range 3.20 × 10-4 to 0.8 μg mL-1and 1.33 × 10-3 to 4.0 μg mL-1, for AMI and FUR, respectively, with a detection limit of 9.62 × 10-5 and 4.01 × 10-4 μg mL-1 (S/N = 3). The commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed method is successfully applied to the determination of both drugs in pharmaceutical formulations.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Masi, Adriana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Materia
AMILORIDE
FUROSEMIDE
NYLON MEMBRANE
PHARMACEUTICAL FORMULATION
SOLID-PHASE EXTRACTION
SPECTROFLUORIMETRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92812
Acceder
id CONICETDig_7feb01996ad3f95a79269d5d060cc5fe
oai_identifier_str oai:ri.conicet.gov.ar:11336/92812
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samplesPeralta, Cecilia MarianaFernandez, Liliana PatriciaMasi, Adriana NoemiAMILORIDEFUROSEMIDENYLON MEMBRANEPHARMACEUTICAL FORMULATIONSOLID-PHASE EXTRACTIONSPECTROFLUORIMETRYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new, simple and highly sensitive method for spectrofluorimetric determination of amiloride (AMI) and furosemide (FUR) in pharmaceuticals is presented. The proposed method is based on the separation of AMI from FUR by solid-phase extraction using a nylon membrane, followed by spectrofluorimetric determination of both drugs, on the solid surface and the filtered aqueous solution, respectively. AMI shows low native fluorescence, but its separation-preconcentration by immobilization (solid-phase extraction) on nylon membrane surface provides a considerable enhancement in fluorescence intensity. The fluorescence determination is carried out at λex = 237, λem = 415 nm for FUR; and λex = 365, λem = 406 nm for AMI. The calibration graphs are linear in the range 3.20 × 10-4 to 0.8 μg mL-1and 1.33 × 10-3 to 4.0 μg mL-1, for AMI and FUR, respectively, with a detection limit of 9.62 × 10-5 and 4.01 × 10-4 μg mL-1 (S/N = 3). The commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed method is successfully applied to the determination of both drugs in pharmaceutical formulations.Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaFil: Masi, Adriana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaElsevier Science2010-02-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92812Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi; A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples; Elsevier Science; Analytica Chimica Acta; 661; 1; 21-2-2010; 85-900003-2670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2009.12.015info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000326700901695Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:03:58Zoai:ri.conicet.gov.ar:11336/92812instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:03:58.665CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples
title A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples
spellingShingle A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples
Peralta, Cecilia Mariana
AMILORIDE
FUROSEMIDE
NYLON MEMBRANE
PHARMACEUTICAL FORMULATION
SOLID-PHASE EXTRACTION
SPECTROFLUORIMETRY
title_short A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples
title_full A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples
title_fullStr A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples
title_full_unstemmed A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples
title_sort A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples
dc.creator.none.fl_str_mv Peralta, Cecilia Mariana
Fernandez, Liliana Patricia
Masi, Adriana Noemi
author Peralta, Cecilia Mariana
author_facet Peralta, Cecilia Mariana
Fernandez, Liliana Patricia
Masi, Adriana Noemi
author_role author
author2 Fernandez, Liliana Patricia
Masi, Adriana Noemi
author2_role author
author
dc.subject.none.fl_str_mv AMILORIDE
FUROSEMIDE
NYLON MEMBRANE
PHARMACEUTICAL FORMULATION
SOLID-PHASE EXTRACTION
SPECTROFLUORIMETRY
topic AMILORIDE
FUROSEMIDE
NYLON MEMBRANE
PHARMACEUTICAL FORMULATION
SOLID-PHASE EXTRACTION
SPECTROFLUORIMETRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new, simple and highly sensitive method for spectrofluorimetric determination of amiloride (AMI) and furosemide (FUR) in pharmaceuticals is presented. The proposed method is based on the separation of AMI from FUR by solid-phase extraction using a nylon membrane, followed by spectrofluorimetric determination of both drugs, on the solid surface and the filtered aqueous solution, respectively. AMI shows low native fluorescence, but its separation-preconcentration by immobilization (solid-phase extraction) on nylon membrane surface provides a considerable enhancement in fluorescence intensity. The fluorescence determination is carried out at λex = 237, λem = 415 nm for FUR; and λex = 365, λem = 406 nm for AMI. The calibration graphs are linear in the range 3.20 × 10-4 to 0.8 μg mL-1and 1.33 × 10-3 to 4.0 μg mL-1, for AMI and FUR, respectively, with a detection limit of 9.62 × 10-5 and 4.01 × 10-4 μg mL-1 (S/N = 3). The commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed method is successfully applied to the determination of both drugs in pharmaceutical formulations.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Masi, Adriana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
description A new, simple and highly sensitive method for spectrofluorimetric determination of amiloride (AMI) and furosemide (FUR) in pharmaceuticals is presented. The proposed method is based on the separation of AMI from FUR by solid-phase extraction using a nylon membrane, followed by spectrofluorimetric determination of both drugs, on the solid surface and the filtered aqueous solution, respectively. AMI shows low native fluorescence, but its separation-preconcentration by immobilization (solid-phase extraction) on nylon membrane surface provides a considerable enhancement in fluorescence intensity. The fluorescence determination is carried out at λex = 237, λem = 415 nm for FUR; and λex = 365, λem = 406 nm for AMI. The calibration graphs are linear in the range 3.20 × 10-4 to 0.8 μg mL-1and 1.33 × 10-3 to 4.0 μg mL-1, for AMI and FUR, respectively, with a detection limit of 9.62 × 10-5 and 4.01 × 10-4 μg mL-1 (S/N = 3). The commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed method is successfully applied to the determination of both drugs in pharmaceutical formulations.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92812
Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi; A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples; Elsevier Science; Analytica Chimica Acta; 661; 1; 21-2-2010; 85-90
0003-2670
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92812
identifier_str_mv Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi; A novel application of immobilization on membranes for the separation and spectrofluorimetric quantification of amiloride and furosemide in pharmaceutical samples; Elsevier Science; Analytica Chimica Acta; 661; 1; 21-2-2010; 85-90
0003-2670
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2009.12.015
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000326700901695X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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