Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition

Autores
Bhattacharyya, Tapan; Falconar, Andrew K.; Luquetti, Alejandro O.; Costales, Jaime A.; Grijalva, Mario J.; Lewis, Michael D.; Messenger, Louisa A.; Tran, Trang T.; Ramirez, Juan David; Guhl, Felipe; Carrasco, Hernan J.; Diosque, Patricio; Garcia, Lineth; Litvinov, Sergey V.; Miles, Michael A.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Chagas disease, caused by infection with the protozoan Trypanosoma cruzi, remains a serious public health issue in Latin America. Genetically diverse, the species is sub-divided into six lineages, known as TcI-TcVI, which have disparate geographical and ecological distributions. TcII, TcV, and TcVI are associated with severe human disease in the Southern Cone countries, whereas TcI is associated with cardiomyopathy north of the Amazon. T. cruzi persists as a chronic infection, with cardiac and/or gastrointestinal symptoms developing years or decades after initial infection. Identifying an individual´s history of T. cruzi lineage infection directly by genotyping of the parasite is complicated by the low parasitaemia and sequestration in the host tissues. Methodology/Principal Findings We have applied here serology against lineage-specific epitopes of the T. cruzi surface antigen TSSA, as an indirect approach to allow identification of infecting lineage. Chagasic sera from chronic patients from a range of endemic countries were tested by ELISA against synthetic peptides representing lineage-specific TSSA epitopes bound to avidin-coated ELISA plates via a biotin labelled polyethylene glycol-glycine spacer to increase rotation and ensure each amino acid side chain could freely interact with their antibodies. 79/113 (70%) of samples from Brazil, Bolivia, and Argentina recognised the TSSA epitope common to lineages TcII/TcV/TcVI. Comparison with clinical information showed that a higher proportion of Brazilian TSSApep-II/V/VI responders had ECG abnormalities than non-responders (38% vs 17%; p<0.0001). Among northern chagasic sera 4/20 (20%) from Ecuador reacted with this peptide; 1/12 Venezuelan and 1/34 Colombian samples reacted with TSSApep-IV. In addition, a proposed TcI-specific epitope, described elsewhere, was demonstrated here to be highly conserved across lineages and therefore not applicable to lineage-specific serology. Conclusions/Significance These results demonstrate the considerable potential for synthetic peptide serology to investigate the infection history of individuals, geographical and clinical associations of T. cruzi lineages.
Fil: Bhattacharyya, Tapan. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Falconar, Andrew K.. Universidad del Norte; Colombia
Fil: Luquetti, Alejandro O.. Universidade Federal de Goias; Brasil
Fil: Costales, Jaime A.. Pontificia Universidad Catolica del Ecuador; Ecuador
Fil: Grijalva, Mario J.. Pontificia Universidad Catolica del Ecuador; Ecuador. Ohio University; Estados Unidos
Fil: Lewis, Michael D.. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Messenger, Louisa A.. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Tran, Trang T.. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Ramirez, Juan David. Universidad del Rosario; Colombia
Fil: Guhl, Felipe. Universidad de Los Andes; Venezuela
Fil: Carrasco, Hernan J.. Universidad Central de Venezuela; Venezuela
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Garcia, Lineth. Universidad Mayor de San Simón; Bolivia
Fil: Litvinov, Sergey V.. Aptum Biologics Ltd.; Reino Unido
Fil: Miles, Michael A.. London School of Hygiene and Tropical Medicine; Reino Unido
Materia
CHAGAS DISEASE
SEROLOGY
LINEAGES
GEOGRAPHIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7616
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7616
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognitionBhattacharyya, TapanFalconar, Andrew K.Luquetti, Alejandro O.Costales, Jaime A.Grijalva, Mario J.Lewis, Michael D.Messenger, Louisa A.Tran, Trang T.Ramirez, Juan DavidGuhl, FelipeCarrasco, Hernan J.Diosque, PatricioGarcia, LinethLitvinov, Sergey V.Miles, Michael A.CHAGAS DISEASESEROLOGYLINEAGESGEOGRAPHIChttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background Chagas disease, caused by infection with the protozoan Trypanosoma cruzi, remains a serious public health issue in Latin America. Genetically diverse, the species is sub-divided into six lineages, known as TcI-TcVI, which have disparate geographical and ecological distributions. TcII, TcV, and TcVI are associated with severe human disease in the Southern Cone countries, whereas TcI is associated with cardiomyopathy north of the Amazon. T. cruzi persists as a chronic infection, with cardiac and/or gastrointestinal symptoms developing years or decades after initial infection. Identifying an individual´s history of T. cruzi lineage infection directly by genotyping of the parasite is complicated by the low parasitaemia and sequestration in the host tissues. Methodology/Principal Findings We have applied here serology against lineage-specific epitopes of the T. cruzi surface antigen TSSA, as an indirect approach to allow identification of infecting lineage. Chagasic sera from chronic patients from a range of endemic countries were tested by ELISA against synthetic peptides representing lineage-specific TSSA epitopes bound to avidin-coated ELISA plates via a biotin labelled polyethylene glycol-glycine spacer to increase rotation and ensure each amino acid side chain could freely interact with their antibodies. 79/113 (70%) of samples from Brazil, Bolivia, and Argentina recognised the TSSA epitope common to lineages TcII/TcV/TcVI. Comparison with clinical information showed that a higher proportion of Brazilian TSSApep-II/V/VI responders had ECG abnormalities than non-responders (38% vs 17%; p<0.0001). Among northern chagasic sera 4/20 (20%) from Ecuador reacted with this peptide; 1/12 Venezuelan and 1/34 Colombian samples reacted with TSSApep-IV. In addition, a proposed TcI-specific epitope, described elsewhere, was demonstrated here to be highly conserved across lineages and therefore not applicable to lineage-specific serology. Conclusions/Significance These results demonstrate the considerable potential for synthetic peptide serology to investigate the infection history of individuals, geographical and clinical associations of T. cruzi lineages.Fil: Bhattacharyya, Tapan. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Falconar, Andrew K.. Universidad del Norte; ColombiaFil: Luquetti, Alejandro O.. Universidade Federal de Goias; BrasilFil: Costales, Jaime A.. Pontificia Universidad Catolica del Ecuador; EcuadorFil: Grijalva, Mario J.. Pontificia Universidad Catolica del Ecuador; Ecuador. Ohio University; Estados UnidosFil: Lewis, Michael D.. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Messenger, Louisa A.. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Tran, Trang T.. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Ramirez, Juan David. Universidad del Rosario; ColombiaFil: Guhl, Felipe. Universidad de Los Andes; VenezuelaFil: Carrasco, Hernan J.. Universidad Central de Venezuela; VenezuelaFil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; ArgentinaFil: Garcia, Lineth. Universidad Mayor de San Simón; BoliviaFil: Litvinov, Sergey V.. Aptum Biologics Ltd.; Reino UnidoFil: Miles, Michael A.. London School of Hygiene and Tropical Medicine; Reino UnidoPublic Library Of Science2014-05-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7616Bhattacharyya, Tapan; Falconar, Andrew K.; Luquetti, Alejandro O.; Costales, Jaime A.; Grijalva, Mario J.; et al.; Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition; Public Library Of Science; Neglected Tropical Diseases; 8; 5; 22-5-2014; e28921935-2735enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002892info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031129/info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002892info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:48:27Zoai:ri.conicet.gov.ar:11336/7616instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:48:27.536CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition
title Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition
spellingShingle Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition
Bhattacharyya, Tapan
CHAGAS DISEASE
SEROLOGY
LINEAGES
GEOGRAPHIC
title_short Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition
title_full Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition
title_fullStr Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition
title_full_unstemmed Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition
title_sort Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition
dc.creator.none.fl_str_mv Bhattacharyya, Tapan
Falconar, Andrew K.
Luquetti, Alejandro O.
Costales, Jaime A.
Grijalva, Mario J.
Lewis, Michael D.
Messenger, Louisa A.
Tran, Trang T.
Ramirez, Juan David
Guhl, Felipe
Carrasco, Hernan J.
Diosque, Patricio
Garcia, Lineth
Litvinov, Sergey V.
Miles, Michael A.
author Bhattacharyya, Tapan
author_facet Bhattacharyya, Tapan
Falconar, Andrew K.
Luquetti, Alejandro O.
Costales, Jaime A.
Grijalva, Mario J.
Lewis, Michael D.
Messenger, Louisa A.
Tran, Trang T.
Ramirez, Juan David
Guhl, Felipe
Carrasco, Hernan J.
Diosque, Patricio
Garcia, Lineth
Litvinov, Sergey V.
Miles, Michael A.
author_role author
author2 Falconar, Andrew K.
Luquetti, Alejandro O.
Costales, Jaime A.
Grijalva, Mario J.
Lewis, Michael D.
Messenger, Louisa A.
Tran, Trang T.
Ramirez, Juan David
Guhl, Felipe
Carrasco, Hernan J.
Diosque, Patricio
Garcia, Lineth
Litvinov, Sergey V.
Miles, Michael A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHAGAS DISEASE
SEROLOGY
LINEAGES
GEOGRAPHIC
topic CHAGAS DISEASE
SEROLOGY
LINEAGES
GEOGRAPHIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background Chagas disease, caused by infection with the protozoan Trypanosoma cruzi, remains a serious public health issue in Latin America. Genetically diverse, the species is sub-divided into six lineages, known as TcI-TcVI, which have disparate geographical and ecological distributions. TcII, TcV, and TcVI are associated with severe human disease in the Southern Cone countries, whereas TcI is associated with cardiomyopathy north of the Amazon. T. cruzi persists as a chronic infection, with cardiac and/or gastrointestinal symptoms developing years or decades after initial infection. Identifying an individual´s history of T. cruzi lineage infection directly by genotyping of the parasite is complicated by the low parasitaemia and sequestration in the host tissues. Methodology/Principal Findings We have applied here serology against lineage-specific epitopes of the T. cruzi surface antigen TSSA, as an indirect approach to allow identification of infecting lineage. Chagasic sera from chronic patients from a range of endemic countries were tested by ELISA against synthetic peptides representing lineage-specific TSSA epitopes bound to avidin-coated ELISA plates via a biotin labelled polyethylene glycol-glycine spacer to increase rotation and ensure each amino acid side chain could freely interact with their antibodies. 79/113 (70%) of samples from Brazil, Bolivia, and Argentina recognised the TSSA epitope common to lineages TcII/TcV/TcVI. Comparison with clinical information showed that a higher proportion of Brazilian TSSApep-II/V/VI responders had ECG abnormalities than non-responders (38% vs 17%; p<0.0001). Among northern chagasic sera 4/20 (20%) from Ecuador reacted with this peptide; 1/12 Venezuelan and 1/34 Colombian samples reacted with TSSApep-IV. In addition, a proposed TcI-specific epitope, described elsewhere, was demonstrated here to be highly conserved across lineages and therefore not applicable to lineage-specific serology. Conclusions/Significance These results demonstrate the considerable potential for synthetic peptide serology to investigate the infection history of individuals, geographical and clinical associations of T. cruzi lineages.
Fil: Bhattacharyya, Tapan. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Falconar, Andrew K.. Universidad del Norte; Colombia
Fil: Luquetti, Alejandro O.. Universidade Federal de Goias; Brasil
Fil: Costales, Jaime A.. Pontificia Universidad Catolica del Ecuador; Ecuador
Fil: Grijalva, Mario J.. Pontificia Universidad Catolica del Ecuador; Ecuador. Ohio University; Estados Unidos
Fil: Lewis, Michael D.. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Messenger, Louisa A.. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Tran, Trang T.. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Ramirez, Juan David. Universidad del Rosario; Colombia
Fil: Guhl, Felipe. Universidad de Los Andes; Venezuela
Fil: Carrasco, Hernan J.. Universidad Central de Venezuela; Venezuela
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Garcia, Lineth. Universidad Mayor de San Simón; Bolivia
Fil: Litvinov, Sergey V.. Aptum Biologics Ltd.; Reino Unido
Fil: Miles, Michael A.. London School of Hygiene and Tropical Medicine; Reino Unido
description Background Chagas disease, caused by infection with the protozoan Trypanosoma cruzi, remains a serious public health issue in Latin America. Genetically diverse, the species is sub-divided into six lineages, known as TcI-TcVI, which have disparate geographical and ecological distributions. TcII, TcV, and TcVI are associated with severe human disease in the Southern Cone countries, whereas TcI is associated with cardiomyopathy north of the Amazon. T. cruzi persists as a chronic infection, with cardiac and/or gastrointestinal symptoms developing years or decades after initial infection. Identifying an individual´s history of T. cruzi lineage infection directly by genotyping of the parasite is complicated by the low parasitaemia and sequestration in the host tissues. Methodology/Principal Findings We have applied here serology against lineage-specific epitopes of the T. cruzi surface antigen TSSA, as an indirect approach to allow identification of infecting lineage. Chagasic sera from chronic patients from a range of endemic countries were tested by ELISA against synthetic peptides representing lineage-specific TSSA epitopes bound to avidin-coated ELISA plates via a biotin labelled polyethylene glycol-glycine spacer to increase rotation and ensure each amino acid side chain could freely interact with their antibodies. 79/113 (70%) of samples from Brazil, Bolivia, and Argentina recognised the TSSA epitope common to lineages TcII/TcV/TcVI. Comparison with clinical information showed that a higher proportion of Brazilian TSSApep-II/V/VI responders had ECG abnormalities than non-responders (38% vs 17%; p<0.0001). Among northern chagasic sera 4/20 (20%) from Ecuador reacted with this peptide; 1/12 Venezuelan and 1/34 Colombian samples reacted with TSSApep-IV. In addition, a proposed TcI-specific epitope, described elsewhere, was demonstrated here to be highly conserved across lineages and therefore not applicable to lineage-specific serology. Conclusions/Significance These results demonstrate the considerable potential for synthetic peptide serology to investigate the infection history of individuals, geographical and clinical associations of T. cruzi lineages.
publishDate 2014
dc.date.none.fl_str_mv 2014-05-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7616
Bhattacharyya, Tapan; Falconar, Andrew K.; Luquetti, Alejandro O.; Costales, Jaime A.; Grijalva, Mario J.; et al.; Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition; Public Library Of Science; Neglected Tropical Diseases; 8; 5; 22-5-2014; e2892
1935-2735
url http://hdl.handle.net/11336/7616
identifier_str_mv Bhattacharyya, Tapan; Falconar, Andrew K.; Luquetti, Alejandro O.; Costales, Jaime A.; Grijalva, Mario J.; et al.; Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition; Public Library Of Science; Neglected Tropical Diseases; 8; 5; 22-5-2014; e2892
1935-2735
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002892
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031129/
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002892
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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