Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions

Autores
Cordero Gabrielli, Paula Verónica; Ferreyra, Darío D.; Pérez, María Eugenia; Alvarez, María Gabriela; Durantini, Edgardo Néstor
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Photocytotoxic activity sensitized by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was investigated in Candida albicans under different culture conditions. Planktonic cells incubated with 2.5 µM TAPC were eradicated after 5 min irradiation with white light.Studies in the presence of reactive oxygen species scavengers indicated the involvement of mainly a type II mechanism. Furthermore, cell growth of C. albicans was suppressed in the presence of 5 µM TAPC. A decrease in pseudohyphae survival of 5 log was found after 30 min irradiation. However, the photokilling of this virulence factor reached a 1.5 log reduction in human serum. The uptake of TAPC by pseudohyphae decreased in serum due to the interaction of TAPC with albumin. The binding constant of the TAPC-albumin complex was ~104 M−1, while the bimolecular quenching rate constant was ~1012 s−1 M−1, indicating that this process occurred through a static process. Thus, the photoinactivation of C. albicans was considerably decreased in the presence of albumin. A reduction of 2 log in cell survival was observed using 4.5% albumin and 30 min irradiation. The results allow optimizing the best conditions to inactivate C. albicans under different culture conditions.
Fil: Cordero Gabrielli, Paula Verónica. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Ferreyra, Darío D.. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Pérez, María Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Materia
CHLORIN
PHOTOSENSITIZER
CANDIDA ALBICANS
PSEUDOHYPHAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/165672
Acceder
id CONICETDig_7f64166c9f0a4acea93c18f04385f153
oai_identifier_str oai:ri.conicet.gov.ar:11336/165672
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditionsCordero Gabrielli, Paula VerónicaFerreyra, Darío D.Pérez, María EugeniaAlvarez, María GabrielaDurantini, Edgardo NéstorCHLORINPHOTOSENSITIZERCANDIDA ALBICANSPSEUDOHYPHAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Photocytotoxic activity sensitized by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was investigated in Candida albicans under different culture conditions. Planktonic cells incubated with 2.5 µM TAPC were eradicated after 5 min irradiation with white light.Studies in the presence of reactive oxygen species scavengers indicated the involvement of mainly a type II mechanism. Furthermore, cell growth of C. albicans was suppressed in the presence of 5 µM TAPC. A decrease in pseudohyphae survival of 5 log was found after 30 min irradiation. However, the photokilling of this virulence factor reached a 1.5 log reduction in human serum. The uptake of TAPC by pseudohyphae decreased in serum due to the interaction of TAPC with albumin. The binding constant of the TAPC-albumin complex was ~104 M−1, while the bimolecular quenching rate constant was ~1012 s−1 M−1, indicating that this process occurred through a static process. Thus, the photoinactivation of C. albicans was considerably decreased in the presence of albumin. A reduction of 2 log in cell survival was observed using 4.5% albumin and 30 min irradiation. The results allow optimizing the best conditions to inactivate C. albicans under different culture conditions.Fil: Cordero Gabrielli, Paula Verónica. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Ferreyra, Darío D.. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Pérez, María Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaMDPI2021-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/165672Cordero Gabrielli, Paula Verónica; Ferreyra, Darío D.; Pérez, María Eugenia; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions; MDPI; Photochem; 1; 3; 12-2021; 505-5222673-7256CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/photochem1030033info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-7256/1/3/33info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:43:18Zoai:ri.conicet.gov.ar:11336/165672instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:43:19.149CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions
title Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions
spellingShingle Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions
Cordero Gabrielli, Paula Verónica
CHLORIN
PHOTOSENSITIZER
CANDIDA ALBICANS
PSEUDOHYPHAE
title_short Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions
title_full Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions
title_fullStr Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions
title_full_unstemmed Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions
title_sort Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions
dc.creator.none.fl_str_mv Cordero Gabrielli, Paula Verónica
Ferreyra, Darío D.
Pérez, María Eugenia
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author Cordero Gabrielli, Paula Verónica
author_facet Cordero Gabrielli, Paula Verónica
Ferreyra, Darío D.
Pérez, María Eugenia
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author_role author
author2 Ferreyra, Darío D.
Pérez, María Eugenia
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author2_role author
author
author
author
dc.subject.none.fl_str_mv CHLORIN
PHOTOSENSITIZER
CANDIDA ALBICANS
PSEUDOHYPHAE
topic CHLORIN
PHOTOSENSITIZER
CANDIDA ALBICANS
PSEUDOHYPHAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Photocytotoxic activity sensitized by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was investigated in Candida albicans under different culture conditions. Planktonic cells incubated with 2.5 µM TAPC were eradicated after 5 min irradiation with white light.Studies in the presence of reactive oxygen species scavengers indicated the involvement of mainly a type II mechanism. Furthermore, cell growth of C. albicans was suppressed in the presence of 5 µM TAPC. A decrease in pseudohyphae survival of 5 log was found after 30 min irradiation. However, the photokilling of this virulence factor reached a 1.5 log reduction in human serum. The uptake of TAPC by pseudohyphae decreased in serum due to the interaction of TAPC with albumin. The binding constant of the TAPC-albumin complex was ~104 M−1, while the bimolecular quenching rate constant was ~1012 s−1 M−1, indicating that this process occurred through a static process. Thus, the photoinactivation of C. albicans was considerably decreased in the presence of albumin. A reduction of 2 log in cell survival was observed using 4.5% albumin and 30 min irradiation. The results allow optimizing the best conditions to inactivate C. albicans under different culture conditions.
Fil: Cordero Gabrielli, Paula Verónica. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Ferreyra, Darío D.. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Pérez, María Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
description Photocytotoxic activity sensitized by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was investigated in Candida albicans under different culture conditions. Planktonic cells incubated with 2.5 µM TAPC were eradicated after 5 min irradiation with white light.Studies in the presence of reactive oxygen species scavengers indicated the involvement of mainly a type II mechanism. Furthermore, cell growth of C. albicans was suppressed in the presence of 5 µM TAPC. A decrease in pseudohyphae survival of 5 log was found after 30 min irradiation. However, the photokilling of this virulence factor reached a 1.5 log reduction in human serum. The uptake of TAPC by pseudohyphae decreased in serum due to the interaction of TAPC with albumin. The binding constant of the TAPC-albumin complex was ~104 M−1, while the bimolecular quenching rate constant was ~1012 s−1 M−1, indicating that this process occurred through a static process. Thus, the photoinactivation of C. albicans was considerably decreased in the presence of albumin. A reduction of 2 log in cell survival was observed using 4.5% albumin and 30 min irradiation. The results allow optimizing the best conditions to inactivate C. albicans under different culture conditions.
publishDate 2021
dc.date.none.fl_str_mv 2021-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/165672
Cordero Gabrielli, Paula Verónica; Ferreyra, Darío D.; Pérez, María Eugenia; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions; MDPI; Photochem; 1; 3; 12-2021; 505-522
2673-7256
CONICET Digital
CONICET
url http://hdl.handle.net/11336/165672
identifier_str_mv Cordero Gabrielli, Paula Verónica; Ferreyra, Darío D.; Pérez, María Eugenia; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions; MDPI; Photochem; 1; 3; 12-2021; 505-522
2673-7256
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/photochem1030033
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-7256/1/3/33
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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