Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions
- Autores
- Cordero Gabrielli, Paula Verónica; Ferreyra, Darío D.; Pérez, María Eugenia; Alvarez, María Gabriela; Durantini, Edgardo Néstor
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Photocytotoxic activity sensitized by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was investigated in Candida albicans under different culture conditions. Planktonic cells incubated with 2.5 µM TAPC were eradicated after 5 min irradiation with white light.Studies in the presence of reactive oxygen species scavengers indicated the involvement of mainly a type II mechanism. Furthermore, cell growth of C. albicans was suppressed in the presence of 5 µM TAPC. A decrease in pseudohyphae survival of 5 log was found after 30 min irradiation. However, the photokilling of this virulence factor reached a 1.5 log reduction in human serum. The uptake of TAPC by pseudohyphae decreased in serum due to the interaction of TAPC with albumin. The binding constant of the TAPC-albumin complex was ~104 M−1, while the bimolecular quenching rate constant was ~1012 s−1 M−1, indicating that this process occurred through a static process. Thus, the photoinactivation of C. albicans was considerably decreased in the presence of albumin. A reduction of 2 log in cell survival was observed using 4.5% albumin and 30 min irradiation. The results allow optimizing the best conditions to inactivate C. albicans under different culture conditions.
Fil: Cordero Gabrielli, Paula Verónica. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Ferreyra, Darío D.. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Pérez, María Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina - Materia
-
CHLORIN
PHOTOSENSITIZER
CANDIDA ALBICANS
PSEUDOHYPHAE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/165672
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Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditionsCordero Gabrielli, Paula VerónicaFerreyra, Darío D.Pérez, María EugeniaAlvarez, María GabrielaDurantini, Edgardo NéstorCHLORINPHOTOSENSITIZERCANDIDA ALBICANSPSEUDOHYPHAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Photocytotoxic activity sensitized by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was investigated in Candida albicans under different culture conditions. Planktonic cells incubated with 2.5 µM TAPC were eradicated after 5 min irradiation with white light.Studies in the presence of reactive oxygen species scavengers indicated the involvement of mainly a type II mechanism. Furthermore, cell growth of C. albicans was suppressed in the presence of 5 µM TAPC. A decrease in pseudohyphae survival of 5 log was found after 30 min irradiation. However, the photokilling of this virulence factor reached a 1.5 log reduction in human serum. The uptake of TAPC by pseudohyphae decreased in serum due to the interaction of TAPC with albumin. The binding constant of the TAPC-albumin complex was ~104 M−1, while the bimolecular quenching rate constant was ~1012 s−1 M−1, indicating that this process occurred through a static process. Thus, the photoinactivation of C. albicans was considerably decreased in the presence of albumin. A reduction of 2 log in cell survival was observed using 4.5% albumin and 30 min irradiation. The results allow optimizing the best conditions to inactivate C. albicans under different culture conditions.Fil: Cordero Gabrielli, Paula Verónica. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Ferreyra, Darío D.. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Pérez, María Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaMDPI2021-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/165672Cordero Gabrielli, Paula Verónica; Ferreyra, Darío D.; Pérez, María Eugenia; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions; MDPI; Photochem; 1; 3; 12-2021; 505-5222673-7256CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/photochem1030033info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-7256/1/3/33info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:24Zoai:ri.conicet.gov.ar:11336/165672instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:25.215CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions |
title |
Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions |
spellingShingle |
Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions Cordero Gabrielli, Paula Verónica CHLORIN PHOTOSENSITIZER CANDIDA ALBICANS PSEUDOHYPHAE |
title_short |
Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions |
title_full |
Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions |
title_fullStr |
Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions |
title_full_unstemmed |
Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions |
title_sort |
Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions |
dc.creator.none.fl_str_mv |
Cordero Gabrielli, Paula Verónica Ferreyra, Darío D. Pérez, María Eugenia Alvarez, María Gabriela Durantini, Edgardo Néstor |
author |
Cordero Gabrielli, Paula Verónica |
author_facet |
Cordero Gabrielli, Paula Verónica Ferreyra, Darío D. Pérez, María Eugenia Alvarez, María Gabriela Durantini, Edgardo Néstor |
author_role |
author |
author2 |
Ferreyra, Darío D. Pérez, María Eugenia Alvarez, María Gabriela Durantini, Edgardo Néstor |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
CHLORIN PHOTOSENSITIZER CANDIDA ALBICANS PSEUDOHYPHAE |
topic |
CHLORIN PHOTOSENSITIZER CANDIDA ALBICANS PSEUDOHYPHAE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Photocytotoxic activity sensitized by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was investigated in Candida albicans under different culture conditions. Planktonic cells incubated with 2.5 µM TAPC were eradicated after 5 min irradiation with white light.Studies in the presence of reactive oxygen species scavengers indicated the involvement of mainly a type II mechanism. Furthermore, cell growth of C. albicans was suppressed in the presence of 5 µM TAPC. A decrease in pseudohyphae survival of 5 log was found after 30 min irradiation. However, the photokilling of this virulence factor reached a 1.5 log reduction in human serum. The uptake of TAPC by pseudohyphae decreased in serum due to the interaction of TAPC with albumin. The binding constant of the TAPC-albumin complex was ~104 M−1, while the bimolecular quenching rate constant was ~1012 s−1 M−1, indicating that this process occurred through a static process. Thus, the photoinactivation of C. albicans was considerably decreased in the presence of albumin. A reduction of 2 log in cell survival was observed using 4.5% albumin and 30 min irradiation. The results allow optimizing the best conditions to inactivate C. albicans under different culture conditions. Fil: Cordero Gabrielli, Paula Verónica. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina Fil: Ferreyra, Darío D.. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina Fil: Pérez, María Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina |
description |
Photocytotoxic activity sensitized by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was investigated in Candida albicans under different culture conditions. Planktonic cells incubated with 2.5 µM TAPC were eradicated after 5 min irradiation with white light.Studies in the presence of reactive oxygen species scavengers indicated the involvement of mainly a type II mechanism. Furthermore, cell growth of C. albicans was suppressed in the presence of 5 µM TAPC. A decrease in pseudohyphae survival of 5 log was found after 30 min irradiation. However, the photokilling of this virulence factor reached a 1.5 log reduction in human serum. The uptake of TAPC by pseudohyphae decreased in serum due to the interaction of TAPC with albumin. The binding constant of the TAPC-albumin complex was ~104 M−1, while the bimolecular quenching rate constant was ~1012 s−1 M−1, indicating that this process occurred through a static process. Thus, the photoinactivation of C. albicans was considerably decreased in the presence of albumin. A reduction of 2 log in cell survival was observed using 4.5% albumin and 30 min irradiation. The results allow optimizing the best conditions to inactivate C. albicans under different culture conditions. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/165672 Cordero Gabrielli, Paula Verónica; Ferreyra, Darío D.; Pérez, María Eugenia; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions; MDPI; Photochem; 1; 3; 12-2021; 505-522 2673-7256 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/165672 |
identifier_str_mv |
Cordero Gabrielli, Paula Verónica; Ferreyra, Darío D.; Pérez, María Eugenia; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic effect of 5,10,15,20-tetrakis[4-(3-N,N-imethylaminopropoxy)phenyl]chlorin towards the human pathogen Candida albicans under different culture conditions; MDPI; Photochem; 1; 3; 12-2021; 505-522 2673-7256 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3390/photochem1030033 info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-7256/1/3/33 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614050976104448 |
score |
13.070432 |