Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells

Autores
Di Palma, María Albana; Alvarez, María Gabriela; Durantini, Edgardo Néstor
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The photoreaction type I/type II pathways mediated by zinc(II) 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]phthalocyanine (ZnPPc4+) was studied in Candida albicans cells. This photosensitizer was strongly bound to C. albicans cells at short times. After 30 min irradiation, 5 μM ZnPPc4+ produced ~5 log decrease in cell viability. Different probes were used to detect reactive oxygen species (ROS) in cell suspensions (~106 CFU mL-1). Singlet molecular oxygen, O2(1Δg), was observed by the reaction with 9,10-dimethylanthracene (DMA) and tetrasodium 2,2-(anthracene-9,10-diyl)bis(methylmalonate) (ABMM), whereas the nitro blue tetrazolium (NBT) method was used to sense superoxide anion radical (O2·-). Moreover, the effects produced by an anoxic atmosphere and cell suspensions in D2O, as well as the addition of sodium azide and mannitol as ROS trapping were evaluated in the PDI of C. albicans. These investigation indicates that O2(1Δg) is generated in the cells, although a minor extension other radical species can also be involved in the PDI of C. albicans mediated by ZnPPc4+.
Fil: Di Palma, María Albana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
PHTHALOCYANINE
PHOTODYNAMIC INACTIVATION
MECHANISM
CANDIDA ALBICANS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180154
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/180154
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans CellsDi Palma, María AlbanaAlvarez, María GabrielaDurantini, Edgardo NéstorPHTHALOCYANINEPHOTODYNAMIC INACTIVATIONMECHANISMCANDIDA ALBICANShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The photoreaction type I/type II pathways mediated by zinc(II) 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]phthalocyanine (ZnPPc4+) was studied in Candida albicans cells. This photosensitizer was strongly bound to C. albicans cells at short times. After 30 min irradiation, 5 μM ZnPPc4+ produced ~5 log decrease in cell viability. Different probes were used to detect reactive oxygen species (ROS) in cell suspensions (~106 CFU mL-1). Singlet molecular oxygen, O2(1Δg), was observed by the reaction with 9,10-dimethylanthracene (DMA) and tetrasodium 2,2-(anthracene-9,10-diyl)bis(methylmalonate) (ABMM), whereas the nitro blue tetrazolium (NBT) method was used to sense superoxide anion radical (O2·-). Moreover, the effects produced by an anoxic atmosphere and cell suspensions in D2O, as well as the addition of sodium azide and mannitol as ROS trapping were evaluated in the PDI of C. albicans. These investigation indicates that O2(1Δg) is generated in the cells, although a minor extension other radical species can also be involved in the PDI of C. albicans mediated by ZnPPc4+.Fil: Di Palma, María Albana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaWiley Blackwell Publishing, Inc2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180154Di Palma, María Albana; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 91; 5; 7-2015; 1203-12090031-8655CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/php.12483info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:18Zoai:ri.conicet.gov.ar:11336/180154instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:18.695CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells
title Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells
spellingShingle Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells
Di Palma, María Albana
PHTHALOCYANINE
PHOTODYNAMIC INACTIVATION
MECHANISM
CANDIDA ALBICANS
title_short Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells
title_full Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells
title_fullStr Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells
title_full_unstemmed Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells
title_sort Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells
dc.creator.none.fl_str_mv Di Palma, María Albana
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author Di Palma, María Albana
author_facet Di Palma, María Albana
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author_role author
author2 Alvarez, María Gabriela
Durantini, Edgardo Néstor
author2_role author
author
dc.subject.none.fl_str_mv PHTHALOCYANINE
PHOTODYNAMIC INACTIVATION
MECHANISM
CANDIDA ALBICANS
topic PHTHALOCYANINE
PHOTODYNAMIC INACTIVATION
MECHANISM
CANDIDA ALBICANS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The photoreaction type I/type II pathways mediated by zinc(II) 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]phthalocyanine (ZnPPc4+) was studied in Candida albicans cells. This photosensitizer was strongly bound to C. albicans cells at short times. After 30 min irradiation, 5 μM ZnPPc4+ produced ~5 log decrease in cell viability. Different probes were used to detect reactive oxygen species (ROS) in cell suspensions (~106 CFU mL-1). Singlet molecular oxygen, O2(1Δg), was observed by the reaction with 9,10-dimethylanthracene (DMA) and tetrasodium 2,2-(anthracene-9,10-diyl)bis(methylmalonate) (ABMM), whereas the nitro blue tetrazolium (NBT) method was used to sense superoxide anion radical (O2·-). Moreover, the effects produced by an anoxic atmosphere and cell suspensions in D2O, as well as the addition of sodium azide and mannitol as ROS trapping were evaluated in the PDI of C. albicans. These investigation indicates that O2(1Δg) is generated in the cells, although a minor extension other radical species can also be involved in the PDI of C. albicans mediated by ZnPPc4+.
Fil: Di Palma, María Albana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description The photoreaction type I/type II pathways mediated by zinc(II) 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]phthalocyanine (ZnPPc4+) was studied in Candida albicans cells. This photosensitizer was strongly bound to C. albicans cells at short times. After 30 min irradiation, 5 μM ZnPPc4+ produced ~5 log decrease in cell viability. Different probes were used to detect reactive oxygen species (ROS) in cell suspensions (~106 CFU mL-1). Singlet molecular oxygen, O2(1Δg), was observed by the reaction with 9,10-dimethylanthracene (DMA) and tetrasodium 2,2-(anthracene-9,10-diyl)bis(methylmalonate) (ABMM), whereas the nitro blue tetrazolium (NBT) method was used to sense superoxide anion radical (O2·-). Moreover, the effects produced by an anoxic atmosphere and cell suspensions in D2O, as well as the addition of sodium azide and mannitol as ROS trapping were evaluated in the PDI of C. albicans. These investigation indicates that O2(1Δg) is generated in the cells, although a minor extension other radical species can also be involved in the PDI of C. albicans mediated by ZnPPc4+.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180154
Di Palma, María Albana; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 91; 5; 7-2015; 1203-1209
0031-8655
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180154
identifier_str_mv Di Palma, María Albana; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 91; 5; 7-2015; 1203-1209
0031-8655
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/php.12483
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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