Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress

Autores
Fernandez Murga, María Leonor; Font, Graciela Maria; Disalvo, Anibal E.
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The zeta potential of Lactobacillus acidophilux CRL 640, a measure of the net distribution of electrical charges on the bacterial surface, is a function of the glucose concentration in the growing media. With 2% glucose, cells in the stationary phase showed a zeta potential of -45 ± 2 mV. With these cells, the zeta potential after freezing and thawing decreased to -32 ± 2 mV and there was a decrease in viability. The changes in the surface potential correlated with damage to the cell surface as shown by electron microscopy. Freeze-thawed cells incubated in a rich medium recovered a zeta potential of -38 ± 2 mV without cell growth. L. acidophilus CRL 640 showed the same value of surface potential as control cells when they were frozen and thawed in 2 M glycerol.
Fil: Fernandez Murga, María Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Disalvo, Anibal E.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Freeze-Thawing
Lactobacillus Acidophilus
Surface Potential
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/51619
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stressFernandez Murga, María LeonorFont, Graciela MariaDisalvo, Anibal E.Freeze-ThawingLactobacillus AcidophilusSurface Potentialhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The zeta potential of Lactobacillus acidophilux CRL 640, a measure of the net distribution of electrical charges on the bacterial surface, is a function of the glucose concentration in the growing media. With 2% glucose, cells in the stationary phase showed a zeta potential of -45 ± 2 mV. With these cells, the zeta potential after freezing and thawing decreased to -32 ± 2 mV and there was a decrease in viability. The changes in the surface potential correlated with damage to the cell surface as shown by electron microscopy. Freeze-thawed cells incubated in a rich medium recovered a zeta potential of -38 ± 2 mV without cell growth. L. acidophilus CRL 640 showed the same value of surface potential as control cells when they were frozen and thawed in 2 M glycerol.Fil: Fernandez Murga, María Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Disalvo, Anibal E.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaAcademic Press Inc Elsevier Science2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/51619Fernandez Murga, María Leonor; Font, Graciela Maria; Disalvo, Anibal E.; Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress; Academic Press Inc Elsevier Science; Cryobiology; 41; 1; 12-2000; 10-160011-22401090-2392CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1006/cryo.2000.2259info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224000922594info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:09:46Zoai:ri.conicet.gov.ar:11336/51619instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:09:46.472CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress
title Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress
spellingShingle Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress
Fernandez Murga, María Leonor
Freeze-Thawing
Lactobacillus Acidophilus
Surface Potential
title_short Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress
title_full Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress
title_fullStr Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress
title_full_unstemmed Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress
title_sort Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress
dc.creator.none.fl_str_mv Fernandez Murga, María Leonor
Font, Graciela Maria
Disalvo, Anibal E.
author Fernandez Murga, María Leonor
author_facet Fernandez Murga, María Leonor
Font, Graciela Maria
Disalvo, Anibal E.
author_role author
author2 Font, Graciela Maria
Disalvo, Anibal E.
author2_role author
author
dc.subject.none.fl_str_mv Freeze-Thawing
Lactobacillus Acidophilus
Surface Potential
topic Freeze-Thawing
Lactobacillus Acidophilus
Surface Potential
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The zeta potential of Lactobacillus acidophilux CRL 640, a measure of the net distribution of electrical charges on the bacterial surface, is a function of the glucose concentration in the growing media. With 2% glucose, cells in the stationary phase showed a zeta potential of -45 ± 2 mV. With these cells, the zeta potential after freezing and thawing decreased to -32 ± 2 mV and there was a decrease in viability. The changes in the surface potential correlated with damage to the cell surface as shown by electron microscopy. Freeze-thawed cells incubated in a rich medium recovered a zeta potential of -38 ± 2 mV without cell growth. L. acidophilus CRL 640 showed the same value of surface potential as control cells when they were frozen and thawed in 2 M glycerol.
Fil: Fernandez Murga, María Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Disalvo, Anibal E.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description The zeta potential of Lactobacillus acidophilux CRL 640, a measure of the net distribution of electrical charges on the bacterial surface, is a function of the glucose concentration in the growing media. With 2% glucose, cells in the stationary phase showed a zeta potential of -45 ± 2 mV. With these cells, the zeta potential after freezing and thawing decreased to -32 ± 2 mV and there was a decrease in viability. The changes in the surface potential correlated with damage to the cell surface as shown by electron microscopy. Freeze-thawed cells incubated in a rich medium recovered a zeta potential of -38 ± 2 mV without cell growth. L. acidophilus CRL 640 showed the same value of surface potential as control cells when they were frozen and thawed in 2 M glycerol.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/51619
Fernandez Murga, María Leonor; Font, Graciela Maria; Disalvo, Anibal E.; Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress; Academic Press Inc Elsevier Science; Cryobiology; 41; 1; 12-2000; 10-16
0011-2240
1090-2392
CONICET Digital
CONICET
url http://hdl.handle.net/11336/51619
identifier_str_mv Fernandez Murga, María Leonor; Font, Graciela Maria; Disalvo, Anibal E.; Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress; Academic Press Inc Elsevier Science; Cryobiology; 41; 1; 12-2000; 10-16
0011-2240
1090-2392
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1006/cryo.2000.2259
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224000922594
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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