Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus
- Autores
- Lorca, Graciela L.; Font, Graciela Maria; Ljungh, Asa
- Año de publicación
- 2002
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation.
Fil: Lorca, Graciela L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Lund University; Suecia
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ljungh, Asa. Lund University; Suecia - Materia
- Lactobacillus Acidophilus
- Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/56042
Ver los metadatos del registro completo
id |
CONICETDig_7ae4184dcb01fa48678557b179111a6b |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/56042 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilusLorca, Graciela L.Font, Graciela MariaLjungh, AsaLactobacillus Acidophilushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation.Fil: Lorca, Graciela L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Lund University; SueciaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Ljungh, Asa. Lund University; SueciaKarger2002-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56042Lorca, Graciela L.; Font, Graciela Maria; Ljungh, Asa; Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus; Karger; Journal of Molecular Microbiology and Biotechnology; 4; 6; 12-2002; 525-5321464-18011660-2412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.karger.com/Journal/Home/228391info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:53Zoai:ri.conicet.gov.ar:11336/56042instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:54.072CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus |
title |
Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus |
spellingShingle |
Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus Lorca, Graciela L. Lactobacillus Acidophilus |
title_short |
Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus |
title_full |
Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus |
title_fullStr |
Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus |
title_full_unstemmed |
Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus |
title_sort |
Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus |
dc.creator.none.fl_str_mv |
Lorca, Graciela L. Font, Graciela Maria Ljungh, Asa |
author |
Lorca, Graciela L. |
author_facet |
Lorca, Graciela L. Font, Graciela Maria Ljungh, Asa |
author_role |
author |
author2 |
Font, Graciela Maria Ljungh, Asa |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Lactobacillus Acidophilus |
topic |
Lactobacillus Acidophilus |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation. Fil: Lorca, Graciela L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Lund University; Suecia Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Ljungh, Asa. Lund University; Suecia |
description |
Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/56042 Lorca, Graciela L.; Font, Graciela Maria; Ljungh, Asa; Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus; Karger; Journal of Molecular Microbiology and Biotechnology; 4; 6; 12-2002; 525-532 1464-1801 1660-2412 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/56042 |
identifier_str_mv |
Lorca, Graciela L.; Font, Graciela Maria; Ljungh, Asa; Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus; Karger; Journal of Molecular Microbiology and Biotechnology; 4; 6; 12-2002; 525-532 1464-1801 1660-2412 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.karger.com/Journal/Home/228391 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842269255841611776 |
score |
13.13397 |