Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus

Autores
Lorca, Graciela L.; Font, Graciela Maria; Ljungh, Asa
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation.
Fil: Lorca, Graciela L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Lund University; Suecia
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ljungh, Asa. Lund University; Suecia
Materia
Lactobacillus Acidophilus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56042
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilusLorca, Graciela L.Font, Graciela MariaLjungh, AsaLactobacillus Acidophilushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation.Fil: Lorca, Graciela L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Lund University; SueciaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Ljungh, Asa. Lund University; SueciaKarger2002-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56042Lorca, Graciela L.; Font, Graciela Maria; Ljungh, Asa; Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus; Karger; Journal of Molecular Microbiology and Biotechnology; 4; 6; 12-2002; 525-5321464-18011660-2412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.karger.com/Journal/Home/228391info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:58Zoai:ri.conicet.gov.ar:11336/56042instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:58.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus
title Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus
spellingShingle Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus
Lorca, Graciela L.
Lactobacillus Acidophilus
title_short Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus
title_full Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus
title_fullStr Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus
title_full_unstemmed Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus
title_sort Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus
dc.creator.none.fl_str_mv Lorca, Graciela L.
Font, Graciela Maria
Ljungh, Asa
author Lorca, Graciela L.
author_facet Lorca, Graciela L.
Font, Graciela Maria
Ljungh, Asa
author_role author
author2 Font, Graciela Maria
Ljungh, Asa
author2_role author
author
dc.subject.none.fl_str_mv Lactobacillus Acidophilus
topic Lactobacillus Acidophilus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation.
Fil: Lorca, Graciela L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Lund University; Suecia
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ljungh, Asa. Lund University; Suecia
description Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation.
publishDate 2002
dc.date.none.fl_str_mv 2002-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56042
Lorca, Graciela L.; Font, Graciela Maria; Ljungh, Asa; Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus; Karger; Journal of Molecular Microbiology and Biotechnology; 4; 6; 12-2002; 525-532
1464-1801
1660-2412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56042
identifier_str_mv Lorca, Graciela L.; Font, Graciela Maria; Ljungh, Asa; Characterization of the Protein-Sinthesis Dependent Adaptive Acid Tolerance Response in Lactobacillus acidophilus; Karger; Journal of Molecular Microbiology and Biotechnology; 4; 6; 12-2002; 525-532
1464-1801
1660-2412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.karger.com/Journal/Home/228391
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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