Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field

Autores
Glavic, Alvaro; Honore, Stella Maris; Feijóo, Carmen Gloria; Bastidas, Francisco; Allende, Miguel L.; Mayor, Roberto
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Different types of placodes originate at the anterior border of the neural plate but it is still an unresolved question whether individual placodes arise as distinct ectodermal specializations in situ or whether all or a subset of the placodes originate from a common preplacodal field. We have analyzed the expression and function of the homeoprotein Iro1 in Xenopus and zebrafish embryos, and we have compared its expression with several preplacodal and placodal markers. Our results indicate that the iro1 genes are expressed in the preplacodal region, being one of the earliest markers for this area. We show that an interaction between the neural plate and the epidermis is able to induce the expression of several preplacodal markers, including Xiro1, by a similar mechanism to that previously shown for neural crest induction. In addition, we analyzed the role of BMP in the specification of the preplacodal field by studying the expression of the preplacodal markers Six1, Xiro1, and several specific placodal markers. We experimentally modified the level of BMP activity by three different methods. First, we implanted beads soaked with noggin in early neurula stage Xenopus embryos; second, we injected the mRNA that encodes a dominant negative of the BMP receptor into Xenopus and zebrafish embryos; and third, we grafted cells expressing chordin into zebrafish embryos. The results obtained using all three methods show that a reduction in the level of BMP activity leads to an expansion of the preplacodal and placodal region similar to what has been described for neural crest regions. By using conditional constructs of Xiro1, we performed gain and loss of function experiments. We show that Xiro1 play an important role in the specification of both the preplacodal field as well as individual placodes. We have also used inducible dominant negative and activator constructs of Notch signaling components to analyze the role of these factors on placodal development. Our results indicate that the a precise level of BMP activity is required to induce the neural plate border, including placodes and neural crest cells, that in this border the iro1 gene is activated, and that this activation is required for the specification of the placodes.
Fil: Glavic, Alvaro. Universidad de Chile; Chile
Fil: Honore, Stella Maris. Universidad de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Feijóo, Carmen Gloria. Universidad de Chile; Chile
Fil: Bastidas, Francisco. Universidad de Chile; Chile
Fil: Allende, Miguel L.. Universidad de Chile; Chile
Fil: Mayor, Roberto. Universidad de Chile; Chile. Colegio Universitario de Londres; Reino Unido
Materia
BMP GRADIENT
DELTA
IRO1
NEURAL CREST
NEUROGENESIS
NOTCH
PLACODE INDUCTION
PLACODES
PREPLACODAL FIELD
SIX1
XENOPUS
ZEBRAFISH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95132
Acceder
id CONICETDig_7eeeb496a00b63ac14b5c19b680cf342
oai_identifier_str oai:ri.conicet.gov.ar:11336/95132
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal fieldGlavic, AlvaroHonore, Stella MarisFeijóo, Carmen GloriaBastidas, FranciscoAllende, Miguel L.Mayor, RobertoBMP GRADIENTDELTAIRO1NEURAL CRESTNEUROGENESISNOTCHPLACODE INDUCTIONPLACODESPREPLACODAL FIELDSIX1XENOPUSZEBRAFISHhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Different types of placodes originate at the anterior border of the neural plate but it is still an unresolved question whether individual placodes arise as distinct ectodermal specializations in situ or whether all or a subset of the placodes originate from a common preplacodal field. We have analyzed the expression and function of the homeoprotein Iro1 in Xenopus and zebrafish embryos, and we have compared its expression with several preplacodal and placodal markers. Our results indicate that the iro1 genes are expressed in the preplacodal region, being one of the earliest markers for this area. We show that an interaction between the neural plate and the epidermis is able to induce the expression of several preplacodal markers, including Xiro1, by a similar mechanism to that previously shown for neural crest induction. In addition, we analyzed the role of BMP in the specification of the preplacodal field by studying the expression of the preplacodal markers Six1, Xiro1, and several specific placodal markers. We experimentally modified the level of BMP activity by three different methods. First, we implanted beads soaked with noggin in early neurula stage Xenopus embryos; second, we injected the mRNA that encodes a dominant negative of the BMP receptor into Xenopus and zebrafish embryos; and third, we grafted cells expressing chordin into zebrafish embryos. The results obtained using all three methods show that a reduction in the level of BMP activity leads to an expansion of the preplacodal and placodal region similar to what has been described for neural crest regions. By using conditional constructs of Xiro1, we performed gain and loss of function experiments. We show that Xiro1 play an important role in the specification of both the preplacodal field as well as individual placodes. We have also used inducible dominant negative and activator constructs of Notch signaling components to analyze the role of these factors on placodal development. Our results indicate that the a precise level of BMP activity is required to induce the neural plate border, including placodes and neural crest cells, that in this border the iro1 gene is activated, and that this activation is required for the specification of the placodes.Fil: Glavic, Alvaro. Universidad de Chile; ChileFil: Honore, Stella Maris. Universidad de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Feijóo, Carmen Gloria. Universidad de Chile; ChileFil: Bastidas, Francisco. Universidad de Chile; ChileFil: Allende, Miguel L.. Universidad de Chile; ChileFil: Mayor, Roberto. Universidad de Chile; Chile. Colegio Universitario de Londres; Reino UnidoAcademic Press Inc Elsevier Science2004-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95132Glavic, Alvaro; Honore, Stella Maris; Feijóo, Carmen Gloria; Bastidas, Francisco; Allende, Miguel L.; et al.; Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field; Academic Press Inc Elsevier Science; Developmental Biology; 272; 1; 8-2004; 89-1030012-1606CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2004.04.020info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0012160604003100info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:36:27Zoai:ri.conicet.gov.ar:11336/95132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:36:28.244CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field
title Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field
spellingShingle Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field
Glavic, Alvaro
BMP GRADIENT
DELTA
IRO1
NEURAL CREST
NEUROGENESIS
NOTCH
PLACODE INDUCTION
PLACODES
PREPLACODAL FIELD
SIX1
XENOPUS
ZEBRAFISH
title_short Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field
title_full Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field
title_fullStr Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field
title_full_unstemmed Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field
title_sort Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field
dc.creator.none.fl_str_mv Glavic, Alvaro
Honore, Stella Maris
Feijóo, Carmen Gloria
Bastidas, Francisco
Allende, Miguel L.
Mayor, Roberto
author Glavic, Alvaro
author_facet Glavic, Alvaro
Honore, Stella Maris
Feijóo, Carmen Gloria
Bastidas, Francisco
Allende, Miguel L.
Mayor, Roberto
author_role author
author2 Honore, Stella Maris
Feijóo, Carmen Gloria
Bastidas, Francisco
Allende, Miguel L.
Mayor, Roberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BMP GRADIENT
DELTA
IRO1
NEURAL CREST
NEUROGENESIS
NOTCH
PLACODE INDUCTION
PLACODES
PREPLACODAL FIELD
SIX1
XENOPUS
ZEBRAFISH
topic BMP GRADIENT
DELTA
IRO1
NEURAL CREST
NEUROGENESIS
NOTCH
PLACODE INDUCTION
PLACODES
PREPLACODAL FIELD
SIX1
XENOPUS
ZEBRAFISH
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Different types of placodes originate at the anterior border of the neural plate but it is still an unresolved question whether individual placodes arise as distinct ectodermal specializations in situ or whether all or a subset of the placodes originate from a common preplacodal field. We have analyzed the expression and function of the homeoprotein Iro1 in Xenopus and zebrafish embryos, and we have compared its expression with several preplacodal and placodal markers. Our results indicate that the iro1 genes are expressed in the preplacodal region, being one of the earliest markers for this area. We show that an interaction between the neural plate and the epidermis is able to induce the expression of several preplacodal markers, including Xiro1, by a similar mechanism to that previously shown for neural crest induction. In addition, we analyzed the role of BMP in the specification of the preplacodal field by studying the expression of the preplacodal markers Six1, Xiro1, and several specific placodal markers. We experimentally modified the level of BMP activity by three different methods. First, we implanted beads soaked with noggin in early neurula stage Xenopus embryos; second, we injected the mRNA that encodes a dominant negative of the BMP receptor into Xenopus and zebrafish embryos; and third, we grafted cells expressing chordin into zebrafish embryos. The results obtained using all three methods show that a reduction in the level of BMP activity leads to an expansion of the preplacodal and placodal region similar to what has been described for neural crest regions. By using conditional constructs of Xiro1, we performed gain and loss of function experiments. We show that Xiro1 play an important role in the specification of both the preplacodal field as well as individual placodes. We have also used inducible dominant negative and activator constructs of Notch signaling components to analyze the role of these factors on placodal development. Our results indicate that the a precise level of BMP activity is required to induce the neural plate border, including placodes and neural crest cells, that in this border the iro1 gene is activated, and that this activation is required for the specification of the placodes.
Fil: Glavic, Alvaro. Universidad de Chile; Chile
Fil: Honore, Stella Maris. Universidad de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Feijóo, Carmen Gloria. Universidad de Chile; Chile
Fil: Bastidas, Francisco. Universidad de Chile; Chile
Fil: Allende, Miguel L.. Universidad de Chile; Chile
Fil: Mayor, Roberto. Universidad de Chile; Chile. Colegio Universitario de Londres; Reino Unido
description Different types of placodes originate at the anterior border of the neural plate but it is still an unresolved question whether individual placodes arise as distinct ectodermal specializations in situ or whether all or a subset of the placodes originate from a common preplacodal field. We have analyzed the expression and function of the homeoprotein Iro1 in Xenopus and zebrafish embryos, and we have compared its expression with several preplacodal and placodal markers. Our results indicate that the iro1 genes are expressed in the preplacodal region, being one of the earliest markers for this area. We show that an interaction between the neural plate and the epidermis is able to induce the expression of several preplacodal markers, including Xiro1, by a similar mechanism to that previously shown for neural crest induction. In addition, we analyzed the role of BMP in the specification of the preplacodal field by studying the expression of the preplacodal markers Six1, Xiro1, and several specific placodal markers. We experimentally modified the level of BMP activity by three different methods. First, we implanted beads soaked with noggin in early neurula stage Xenopus embryos; second, we injected the mRNA that encodes a dominant negative of the BMP receptor into Xenopus and zebrafish embryos; and third, we grafted cells expressing chordin into zebrafish embryos. The results obtained using all three methods show that a reduction in the level of BMP activity leads to an expansion of the preplacodal and placodal region similar to what has been described for neural crest regions. By using conditional constructs of Xiro1, we performed gain and loss of function experiments. We show that Xiro1 play an important role in the specification of both the preplacodal field as well as individual placodes. We have also used inducible dominant negative and activator constructs of Notch signaling components to analyze the role of these factors on placodal development. Our results indicate that the a precise level of BMP activity is required to induce the neural plate border, including placodes and neural crest cells, that in this border the iro1 gene is activated, and that this activation is required for the specification of the placodes.
publishDate 2004
dc.date.none.fl_str_mv 2004-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95132
Glavic, Alvaro; Honore, Stella Maris; Feijóo, Carmen Gloria; Bastidas, Francisco; Allende, Miguel L.; et al.; Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field; Academic Press Inc Elsevier Science; Developmental Biology; 272; 1; 8-2004; 89-103
0012-1606
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95132
identifier_str_mv Glavic, Alvaro; Honore, Stella Maris; Feijóo, Carmen Gloria; Bastidas, Francisco; Allende, Miguel L.; et al.; Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field; Academic Press Inc Elsevier Science; Developmental Biology; 272; 1; 8-2004; 89-103
0012-1606
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2004.04.020
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0012160604003100
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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