Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos

Autores
Glavic, Alvaro; Silva, Francisca; Aybar, Manuel Javier; Bastidas, Francisco; Mayor, Roberto
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The neural crest is a population of cells that originates at the interface between the neural plate and non-neural ectoderm. Here, we have analyzed the role that Notch and the homeoprotein Xiro1 play in the specification of the neural crest. We show that Xiro1, Notch and the Notch target gene Hairy2A are all expressed in the neural crest territory, whereas the Notch ligands Delta1 and Serrate are expressed in the cells that surround the prospective crest cells. We have used inducible dominant-negative and activator constructs of both Notch signaling components and Xiro1 to analyze the role of these factors in neural crest specification without interfering with mesodermal or neural plate development. Activation of Xiro1 or Notch signaling led to an enlargement of the neural crest territory, whereas blocking their activity inhibited the expression of neural crest markers. It is known that BMPs are involved in the induction of the neural crest and, thus, we assessed whether these two elements might influence the expression of Bmp4. Activation of Xiro1 and of Notch signaling upregulated. Hairy2A and inhibited Bmp4 transcription during neural crest specification. These results, in conjunction with data from rescue experiments, allow us to propose a model wherein Xiro1 lies upstream of the cascade regulating Delta1 transcription. At the early gastrula stage, the coordinated action of Xiro1, as a positive regulator, and Snail, as a repressor, restricts the expression of Delta1 at the border of the neural crest territory. At the late gastrula stage, Delta1 interacts with Notch to activate Hairy2A in the region of the neural fold. Subsequently, Hairy2A acts as a repressor of Bmp4 transcription, ensuring that levels of Bmp4 optimal for the specification of the neural plate border are attained in this region. Finally, the activity of additional signals (WNTs, FGF and retinoic acid) in this newly defined domain induces the production of neural crest cells. These data also highlight the different roles played by BMP in neural crest specification in chick and Xenopus or zebrafish embryos.
Fil: Glavic, Alvaro. Universidad de Chile; Chile
Fil: Silva, Francisca. Universidad de Chile; Chile
Fil: Aybar, Manuel Javier. Universidad de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bastidas, Francisco. Universidad de Chile; Chile
Fil: Mayor, Roberto. Universidad de Chile; Chile. Colegio Universitario de Londres; Reino Unido
Materia
BMP
DELTA1
HAIRY2
IROQUOIS
MSX
NEURAL CREST
NOTCH SIGNALING
SNAIL
XENOPUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95307
Acceder
id CONICETDig_440c46e7c18b6f0045cc34a27676769e
oai_identifier_str oai:ri.conicet.gov.ar:11336/95307
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryosGlavic, AlvaroSilva, FranciscaAybar, Manuel JavierBastidas, FranciscoMayor, RobertoBMPDELTA1HAIRY2IROQUOISMSXNEURAL CRESTNOTCH SIGNALINGSNAILXENOPUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The neural crest is a population of cells that originates at the interface between the neural plate and non-neural ectoderm. Here, we have analyzed the role that Notch and the homeoprotein Xiro1 play in the specification of the neural crest. We show that Xiro1, Notch and the Notch target gene Hairy2A are all expressed in the neural crest territory, whereas the Notch ligands Delta1 and Serrate are expressed in the cells that surround the prospective crest cells. We have used inducible dominant-negative and activator constructs of both Notch signaling components and Xiro1 to analyze the role of these factors in neural crest specification without interfering with mesodermal or neural plate development. Activation of Xiro1 or Notch signaling led to an enlargement of the neural crest territory, whereas blocking their activity inhibited the expression of neural crest markers. It is known that BMPs are involved in the induction of the neural crest and, thus, we assessed whether these two elements might influence the expression of Bmp4. Activation of Xiro1 and of Notch signaling upregulated. Hairy2A and inhibited Bmp4 transcription during neural crest specification. These results, in conjunction with data from rescue experiments, allow us to propose a model wherein Xiro1 lies upstream of the cascade regulating Delta1 transcription. At the early gastrula stage, the coordinated action of Xiro1, as a positive regulator, and Snail, as a repressor, restricts the expression of Delta1 at the border of the neural crest territory. At the late gastrula stage, Delta1 interacts with Notch to activate Hairy2A in the region of the neural fold. Subsequently, Hairy2A acts as a repressor of Bmp4 transcription, ensuring that levels of Bmp4 optimal for the specification of the neural plate border are attained in this region. Finally, the activity of additional signals (WNTs, FGF and retinoic acid) in this newly defined domain induces the production of neural crest cells. These data also highlight the different roles played by BMP in neural crest specification in chick and Xenopus or zebrafish embryos.Fil: Glavic, Alvaro. Universidad de Chile; ChileFil: Silva, Francisca. Universidad de Chile; ChileFil: Aybar, Manuel Javier. Universidad de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Bastidas, Francisco. Universidad de Chile; ChileFil: Mayor, Roberto. Universidad de Chile; Chile. Colegio Universitario de Londres; Reino UnidoCompany of Biologists2004-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95307Glavic, Alvaro; Silva, Francisca; Aybar, Manuel Javier; Bastidas, Francisco; Mayor, Roberto; Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos; Company of Biologists; Development; 131; 2; 1-2004; 347-3590950-1991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/cgi/content/short/dev.00945v1info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.00945info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:20Zoai:ri.conicet.gov.ar:11336/95307instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:20.397CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos
title Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos
spellingShingle Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos
Glavic, Alvaro
BMP
DELTA1
HAIRY2
IROQUOIS
MSX
NEURAL CREST
NOTCH SIGNALING
SNAIL
XENOPUS
title_short Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos
title_full Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos
title_fullStr Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos
title_full_unstemmed Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos
title_sort Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos
dc.creator.none.fl_str_mv Glavic, Alvaro
Silva, Francisca
Aybar, Manuel Javier
Bastidas, Francisco
Mayor, Roberto
author Glavic, Alvaro
author_facet Glavic, Alvaro
Silva, Francisca
Aybar, Manuel Javier
Bastidas, Francisco
Mayor, Roberto
author_role author
author2 Silva, Francisca
Aybar, Manuel Javier
Bastidas, Francisco
Mayor, Roberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv BMP
DELTA1
HAIRY2
IROQUOIS
MSX
NEURAL CREST
NOTCH SIGNALING
SNAIL
XENOPUS
topic BMP
DELTA1
HAIRY2
IROQUOIS
MSX
NEURAL CREST
NOTCH SIGNALING
SNAIL
XENOPUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The neural crest is a population of cells that originates at the interface between the neural plate and non-neural ectoderm. Here, we have analyzed the role that Notch and the homeoprotein Xiro1 play in the specification of the neural crest. We show that Xiro1, Notch and the Notch target gene Hairy2A are all expressed in the neural crest territory, whereas the Notch ligands Delta1 and Serrate are expressed in the cells that surround the prospective crest cells. We have used inducible dominant-negative and activator constructs of both Notch signaling components and Xiro1 to analyze the role of these factors in neural crest specification without interfering with mesodermal or neural plate development. Activation of Xiro1 or Notch signaling led to an enlargement of the neural crest territory, whereas blocking their activity inhibited the expression of neural crest markers. It is known that BMPs are involved in the induction of the neural crest and, thus, we assessed whether these two elements might influence the expression of Bmp4. Activation of Xiro1 and of Notch signaling upregulated. Hairy2A and inhibited Bmp4 transcription during neural crest specification. These results, in conjunction with data from rescue experiments, allow us to propose a model wherein Xiro1 lies upstream of the cascade regulating Delta1 transcription. At the early gastrula stage, the coordinated action of Xiro1, as a positive regulator, and Snail, as a repressor, restricts the expression of Delta1 at the border of the neural crest territory. At the late gastrula stage, Delta1 interacts with Notch to activate Hairy2A in the region of the neural fold. Subsequently, Hairy2A acts as a repressor of Bmp4 transcription, ensuring that levels of Bmp4 optimal for the specification of the neural plate border are attained in this region. Finally, the activity of additional signals (WNTs, FGF and retinoic acid) in this newly defined domain induces the production of neural crest cells. These data also highlight the different roles played by BMP in neural crest specification in chick and Xenopus or zebrafish embryos.
Fil: Glavic, Alvaro. Universidad de Chile; Chile
Fil: Silva, Francisca. Universidad de Chile; Chile
Fil: Aybar, Manuel Javier. Universidad de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bastidas, Francisco. Universidad de Chile; Chile
Fil: Mayor, Roberto. Universidad de Chile; Chile. Colegio Universitario de Londres; Reino Unido
description The neural crest is a population of cells that originates at the interface between the neural plate and non-neural ectoderm. Here, we have analyzed the role that Notch and the homeoprotein Xiro1 play in the specification of the neural crest. We show that Xiro1, Notch and the Notch target gene Hairy2A are all expressed in the neural crest territory, whereas the Notch ligands Delta1 and Serrate are expressed in the cells that surround the prospective crest cells. We have used inducible dominant-negative and activator constructs of both Notch signaling components and Xiro1 to analyze the role of these factors in neural crest specification without interfering with mesodermal or neural plate development. Activation of Xiro1 or Notch signaling led to an enlargement of the neural crest territory, whereas blocking their activity inhibited the expression of neural crest markers. It is known that BMPs are involved in the induction of the neural crest and, thus, we assessed whether these two elements might influence the expression of Bmp4. Activation of Xiro1 and of Notch signaling upregulated. Hairy2A and inhibited Bmp4 transcription during neural crest specification. These results, in conjunction with data from rescue experiments, allow us to propose a model wherein Xiro1 lies upstream of the cascade regulating Delta1 transcription. At the early gastrula stage, the coordinated action of Xiro1, as a positive regulator, and Snail, as a repressor, restricts the expression of Delta1 at the border of the neural crest territory. At the late gastrula stage, Delta1 interacts with Notch to activate Hairy2A in the region of the neural fold. Subsequently, Hairy2A acts as a repressor of Bmp4 transcription, ensuring that levels of Bmp4 optimal for the specification of the neural plate border are attained in this region. Finally, the activity of additional signals (WNTs, FGF and retinoic acid) in this newly defined domain induces the production of neural crest cells. These data also highlight the different roles played by BMP in neural crest specification in chick and Xenopus or zebrafish embryos.
publishDate 2004
dc.date.none.fl_str_mv 2004-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95307
Glavic, Alvaro; Silva, Francisca; Aybar, Manuel Javier; Bastidas, Francisco; Mayor, Roberto; Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos; Company of Biologists; Development; 131; 2; 1-2004; 347-359
0950-1991
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95307
identifier_str_mv Glavic, Alvaro; Silva, Francisca; Aybar, Manuel Javier; Bastidas, Francisco; Mayor, Roberto; Interplay between Notch signaling and the homeoprotein Xiro1 is required for neural crest induction in Xenopus embryos; Company of Biologists; Development; 131; 2; 1-2004; 347-359
0950-1991
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/cgi/content/short/dev.00945v1
info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.00945
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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