Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells
- Autores
- Raina, Dhruv; Fabris, Fiorella; Morelli, Luis Guillermo; Schroter, Christian
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Signal transduction networks generate characteristic dynamic activities to process extracellular signals and guide cell fate decisions such as to divide or differentiate. The differentiation of pluripotent cells is controlled by FGF/ERK signaling. However, only a few studies have addressed the dynamic activity of the FGF/ERK signaling network in pluripotent cells at high time resolution. Here, we use live cell sensors in wild-type and Fgf4-mutant mouse embryonic stem cells to measure dynamic ERK activity in single cells, for defined ligand concentrations and differentiation states. These sensors reveal pulses of ERK activity. Pulsing patterns are heterogeneous between individual cells. Consecutive pulse sequences occur more frequently than expected from simple stochastic models. Sequences become more prevalent with higher ligand concentration, but are rarer in more differentiated cells. Our results suggest that FGF/ERK signaling operates in the vicinity of a transition point between oscillatory and non-oscillatory dynamics in embryonic stem cells. The resulting heterogeneous dynamic signaling activities add a new dimension to cellular heterogeneity that may be linked to divergent fate decisions in stem cell cultures.
Fil: Raina, Dhruv. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Fabris, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Morelli, Luis Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Schroter, Christian. Institut Max Planck fur Molekulare Physiologie; Alemania - Materia
-
CELL CYCLE
FGF/ERK SIGNALING
MOUSE EMBRYONIC STEM CELLS
OSCILLATIONS
SIGNAL TRANSDUCTION NETWORKS
TIME SERIES ANALYSIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/214294
Ver los metadatos del registro completo
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Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cellsRaina, DhruvFabris, FiorellaMorelli, Luis GuillermoSchroter, ChristianCELL CYCLEFGF/ERK SIGNALINGMOUSE EMBRYONIC STEM CELLSOSCILLATIONSSIGNAL TRANSDUCTION NETWORKSTIME SERIES ANALYSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Signal transduction networks generate characteristic dynamic activities to process extracellular signals and guide cell fate decisions such as to divide or differentiate. The differentiation of pluripotent cells is controlled by FGF/ERK signaling. However, only a few studies have addressed the dynamic activity of the FGF/ERK signaling network in pluripotent cells at high time resolution. Here, we use live cell sensors in wild-type and Fgf4-mutant mouse embryonic stem cells to measure dynamic ERK activity in single cells, for defined ligand concentrations and differentiation states. These sensors reveal pulses of ERK activity. Pulsing patterns are heterogeneous between individual cells. Consecutive pulse sequences occur more frequently than expected from simple stochastic models. Sequences become more prevalent with higher ligand concentration, but are rarer in more differentiated cells. Our results suggest that FGF/ERK signaling operates in the vicinity of a transition point between oscillatory and non-oscillatory dynamics in embryonic stem cells. The resulting heterogeneous dynamic signaling activities add a new dimension to cellular heterogeneity that may be linked to divergent fate decisions in stem cell cultures.Fil: Raina, Dhruv. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Fabris, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Morelli, Luis Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Schroter, Christian. Institut Max Planck fur Molekulare Physiologie; AlemaniaCompany of Biologists2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214294Raina, Dhruv; Fabris, Fiorella; Morelli, Luis Guillermo; Schroter, Christian; Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells; Company of Biologists; Development; 149; 4; 2-2022; 1-390950-1991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1242/dev.199710info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:19:34Zoai:ri.conicet.gov.ar:11336/214294instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:19:35.23CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells |
title |
Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells |
spellingShingle |
Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells Raina, Dhruv CELL CYCLE FGF/ERK SIGNALING MOUSE EMBRYONIC STEM CELLS OSCILLATIONS SIGNAL TRANSDUCTION NETWORKS TIME SERIES ANALYSIS |
title_short |
Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells |
title_full |
Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells |
title_fullStr |
Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells |
title_full_unstemmed |
Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells |
title_sort |
Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells |
dc.creator.none.fl_str_mv |
Raina, Dhruv Fabris, Fiorella Morelli, Luis Guillermo Schroter, Christian |
author |
Raina, Dhruv |
author_facet |
Raina, Dhruv Fabris, Fiorella Morelli, Luis Guillermo Schroter, Christian |
author_role |
author |
author2 |
Fabris, Fiorella Morelli, Luis Guillermo Schroter, Christian |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
CELL CYCLE FGF/ERK SIGNALING MOUSE EMBRYONIC STEM CELLS OSCILLATIONS SIGNAL TRANSDUCTION NETWORKS TIME SERIES ANALYSIS |
topic |
CELL CYCLE FGF/ERK SIGNALING MOUSE EMBRYONIC STEM CELLS OSCILLATIONS SIGNAL TRANSDUCTION NETWORKS TIME SERIES ANALYSIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.3 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Signal transduction networks generate characteristic dynamic activities to process extracellular signals and guide cell fate decisions such as to divide or differentiate. The differentiation of pluripotent cells is controlled by FGF/ERK signaling. However, only a few studies have addressed the dynamic activity of the FGF/ERK signaling network in pluripotent cells at high time resolution. Here, we use live cell sensors in wild-type and Fgf4-mutant mouse embryonic stem cells to measure dynamic ERK activity in single cells, for defined ligand concentrations and differentiation states. These sensors reveal pulses of ERK activity. Pulsing patterns are heterogeneous between individual cells. Consecutive pulse sequences occur more frequently than expected from simple stochastic models. Sequences become more prevalent with higher ligand concentration, but are rarer in more differentiated cells. Our results suggest that FGF/ERK signaling operates in the vicinity of a transition point between oscillatory and non-oscillatory dynamics in embryonic stem cells. The resulting heterogeneous dynamic signaling activities add a new dimension to cellular heterogeneity that may be linked to divergent fate decisions in stem cell cultures. Fil: Raina, Dhruv. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Fabris, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina Fil: Morelli, Luis Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina. Institut Max Planck fur Molekulare Physiologie; Alemania Fil: Schroter, Christian. Institut Max Planck fur Molekulare Physiologie; Alemania |
description |
Signal transduction networks generate characteristic dynamic activities to process extracellular signals and guide cell fate decisions such as to divide or differentiate. The differentiation of pluripotent cells is controlled by FGF/ERK signaling. However, only a few studies have addressed the dynamic activity of the FGF/ERK signaling network in pluripotent cells at high time resolution. Here, we use live cell sensors in wild-type and Fgf4-mutant mouse embryonic stem cells to measure dynamic ERK activity in single cells, for defined ligand concentrations and differentiation states. These sensors reveal pulses of ERK activity. Pulsing patterns are heterogeneous between individual cells. Consecutive pulse sequences occur more frequently than expected from simple stochastic models. Sequences become more prevalent with higher ligand concentration, but are rarer in more differentiated cells. Our results suggest that FGF/ERK signaling operates in the vicinity of a transition point between oscillatory and non-oscillatory dynamics in embryonic stem cells. The resulting heterogeneous dynamic signaling activities add a new dimension to cellular heterogeneity that may be linked to divergent fate decisions in stem cell cultures. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/214294 Raina, Dhruv; Fabris, Fiorella; Morelli, Luis Guillermo; Schroter, Christian; Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells; Company of Biologists; Development; 149; 4; 2-2022; 1-39 0950-1991 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/214294 |
identifier_str_mv |
Raina, Dhruv; Fabris, Fiorella; Morelli, Luis Guillermo; Schroter, Christian; Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells; Company of Biologists; Development; 149; 4; 2-2022; 1-39 0950-1991 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.199710 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Company of Biologists |
publisher.none.fl_str_mv |
Company of Biologists |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083344830300160 |
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13.22299 |