A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
- Autores
- Fagundez, Carol Betiana; Loresi, Monica Alejandra; Ojea Quintana, Marcos Eduardo; Delcourt, Stella Maris; Testa, Roberto; Gogorza, Sebastian J.; Argibay, Pablo
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the present study, we derived stem cells from hatched blastocyst-stage mouse embryos from C57BL/6 strain employing a knockout serum replacement instead of the traditional foetal calf serum, avoiding the use of immunosurgery. Although foetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The cells derived were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage specific embryonic antigen 1 (SSEA-1) and the octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes was better compared with hanging drop method for differentiation by means of embryoid body formation. The mouse embryonic stem cells obtained during this work showed spontaneous differentiation to derivatives of the three germ layers in culture media supplemented with foetal calf serum but not with knockout serum.
Fil: Fagundez, Carol Betiana. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ojea Quintana, Marcos Eduardo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Delcourt, Stella Maris. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Testa, Roberto. Hospital Italiano; Argentina
Fil: Gogorza, Sebastian J.. Hospital Italiano; Argentina
Fil: Argibay, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina - Materia
-
EMBRYONIC STEM CELLS
HATCHED BLASTOCYSTS
EMBRYOID BODY FORMATION
C57BL/6 MOUSE STRAIN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/248042
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A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formationFagundez, Carol BetianaLoresi, Monica AlejandraOjea Quintana, Marcos EduardoDelcourt, Stella MarisTesta, RobertoGogorza, Sebastian J.Argibay, PabloEMBRYONIC STEM CELLSHATCHED BLASTOCYSTSEMBRYOID BODY FORMATIONC57BL/6 MOUSE STRAINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present study, we derived stem cells from hatched blastocyst-stage mouse embryos from C57BL/6 strain employing a knockout serum replacement instead of the traditional foetal calf serum, avoiding the use of immunosurgery. Although foetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The cells derived were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage specific embryonic antigen 1 (SSEA-1) and the octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes was better compared with hanging drop method for differentiation by means of embryoid body formation. The mouse embryonic stem cells obtained during this work showed spontaneous differentiation to derivatives of the three germ layers in culture media supplemented with foetal calf serum but not with knockout serum.Fil: Fagundez, Carol Betiana. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Ojea Quintana, Marcos Eduardo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Delcourt, Stella Maris. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Testa, Roberto. Hospital Italiano; ArgentinaFil: Gogorza, Sebastian J.. Hospital Italiano; ArgentinaFil: Argibay, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/248042Fagundez, Carol Betiana; Loresi, Monica Alejandra; Ojea Quintana, Marcos Eduardo; Delcourt, Stella Maris; Testa, Roberto; et al.; A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation; Academic Press Ltd - Elsevier Science Ltd; Cell Biology International; 33; 11; 1-2013; 1196-12001065-6995CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1065699509001954info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellbi.2009.08.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:43:37Zoai:ri.conicet.gov.ar:11336/248042instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:43:38.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation |
title |
A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation |
spellingShingle |
A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation Fagundez, Carol Betiana EMBRYONIC STEM CELLS HATCHED BLASTOCYSTS EMBRYOID BODY FORMATION C57BL/6 MOUSE STRAIN |
title_short |
A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation |
title_full |
A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation |
title_fullStr |
A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation |
title_full_unstemmed |
A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation |
title_sort |
A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation |
dc.creator.none.fl_str_mv |
Fagundez, Carol Betiana Loresi, Monica Alejandra Ojea Quintana, Marcos Eduardo Delcourt, Stella Maris Testa, Roberto Gogorza, Sebastian J. Argibay, Pablo |
author |
Fagundez, Carol Betiana |
author_facet |
Fagundez, Carol Betiana Loresi, Monica Alejandra Ojea Quintana, Marcos Eduardo Delcourt, Stella Maris Testa, Roberto Gogorza, Sebastian J. Argibay, Pablo |
author_role |
author |
author2 |
Loresi, Monica Alejandra Ojea Quintana, Marcos Eduardo Delcourt, Stella Maris Testa, Roberto Gogorza, Sebastian J. Argibay, Pablo |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
EMBRYONIC STEM CELLS HATCHED BLASTOCYSTS EMBRYOID BODY FORMATION C57BL/6 MOUSE STRAIN |
topic |
EMBRYONIC STEM CELLS HATCHED BLASTOCYSTS EMBRYOID BODY FORMATION C57BL/6 MOUSE STRAIN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In the present study, we derived stem cells from hatched blastocyst-stage mouse embryos from C57BL/6 strain employing a knockout serum replacement instead of the traditional foetal calf serum, avoiding the use of immunosurgery. Although foetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The cells derived were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage specific embryonic antigen 1 (SSEA-1) and the octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes was better compared with hanging drop method for differentiation by means of embryoid body formation. The mouse embryonic stem cells obtained during this work showed spontaneous differentiation to derivatives of the three germ layers in culture media supplemented with foetal calf serum but not with knockout serum. Fil: Fagundez, Carol Betiana. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina Fil: Ojea Quintana, Marcos Eduardo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina Fil: Delcourt, Stella Maris. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina Fil: Testa, Roberto. Hospital Italiano; Argentina Fil: Gogorza, Sebastian J.. Hospital Italiano; Argentina Fil: Argibay, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina |
description |
In the present study, we derived stem cells from hatched blastocyst-stage mouse embryos from C57BL/6 strain employing a knockout serum replacement instead of the traditional foetal calf serum, avoiding the use of immunosurgery. Although foetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The cells derived were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage specific embryonic antigen 1 (SSEA-1) and the octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes was better compared with hanging drop method for differentiation by means of embryoid body formation. The mouse embryonic stem cells obtained during this work showed spontaneous differentiation to derivatives of the three germ layers in culture media supplemented with foetal calf serum but not with knockout serum. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/248042 Fagundez, Carol Betiana; Loresi, Monica Alejandra; Ojea Quintana, Marcos Eduardo; Delcourt, Stella Maris; Testa, Roberto; et al.; A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation; Academic Press Ltd - Elsevier Science Ltd; Cell Biology International; 33; 11; 1-2013; 1196-1200 1065-6995 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/248042 |
identifier_str_mv |
Fagundez, Carol Betiana; Loresi, Monica Alejandra; Ojea Quintana, Marcos Eduardo; Delcourt, Stella Maris; Testa, Roberto; et al.; A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation; Academic Press Ltd - Elsevier Science Ltd; Cell Biology International; 33; 11; 1-2013; 1196-1200 1065-6995 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1065699509001954 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellbi.2009.08.001 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Ltd - Elsevier Science Ltd |
publisher.none.fl_str_mv |
Academic Press Ltd - Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083541190836224 |
score |
13.22299 |