A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation

Autores
Fagundez, Carol Betiana; Loresi, Monica Alejandra; Ojea Quintana, Marcos Eduardo; Delcourt, Stella Maris; Testa, Roberto; Gogorza, Sebastian J.; Argibay, Pablo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study, we derived stem cells from hatched blastocyst-stage mouse embryos from C57BL/6 strain employing a knockout serum replacement instead of the traditional foetal calf serum, avoiding the use of immunosurgery. Although foetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The cells derived were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage specific embryonic antigen 1 (SSEA-1) and the octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes was better compared with hanging drop method for differentiation by means of embryoid body formation. The mouse embryonic stem cells obtained during this work showed spontaneous differentiation to derivatives of the three germ layers in culture media supplemented with foetal calf serum but not with knockout serum.
Fil: Fagundez, Carol Betiana. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ojea Quintana, Marcos Eduardo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Delcourt, Stella Maris. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Testa, Roberto. Hospital Italiano; Argentina
Fil: Gogorza, Sebastian J.. Hospital Italiano; Argentina
Fil: Argibay, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Materia
EMBRYONIC STEM CELLS
HATCHED BLASTOCYSTS
EMBRYOID BODY FORMATION
C57BL/6 MOUSE STRAIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/248042

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network_name_str CONICET Digital (CONICET)
spelling A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formationFagundez, Carol BetianaLoresi, Monica AlejandraOjea Quintana, Marcos EduardoDelcourt, Stella MarisTesta, RobertoGogorza, Sebastian J.Argibay, PabloEMBRYONIC STEM CELLSHATCHED BLASTOCYSTSEMBRYOID BODY FORMATIONC57BL/6 MOUSE STRAINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present study, we derived stem cells from hatched blastocyst-stage mouse embryos from C57BL/6 strain employing a knockout serum replacement instead of the traditional foetal calf serum, avoiding the use of immunosurgery. Although foetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The cells derived were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage specific embryonic antigen 1 (SSEA-1) and the octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes was better compared with hanging drop method for differentiation by means of embryoid body formation. The mouse embryonic stem cells obtained during this work showed spontaneous differentiation to derivatives of the three germ layers in culture media supplemented with foetal calf serum but not with knockout serum.Fil: Fagundez, Carol Betiana. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Ojea Quintana, Marcos Eduardo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Delcourt, Stella Maris. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Testa, Roberto. Hospital Italiano; ArgentinaFil: Gogorza, Sebastian J.. Hospital Italiano; ArgentinaFil: Argibay, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/248042Fagundez, Carol Betiana; Loresi, Monica Alejandra; Ojea Quintana, Marcos Eduardo; Delcourt, Stella Maris; Testa, Roberto; et al.; A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation; Academic Press Ltd - Elsevier Science Ltd; Cell Biology International; 33; 11; 1-2013; 1196-12001065-6995CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1065699509001954info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellbi.2009.08.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:43:37Zoai:ri.conicet.gov.ar:11336/248042instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:43:38.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
title A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
spellingShingle A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
Fagundez, Carol Betiana
EMBRYONIC STEM CELLS
HATCHED BLASTOCYSTS
EMBRYOID BODY FORMATION
C57BL/6 MOUSE STRAIN
title_short A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
title_full A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
title_fullStr A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
title_full_unstemmed A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
title_sort A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
dc.creator.none.fl_str_mv Fagundez, Carol Betiana
Loresi, Monica Alejandra
Ojea Quintana, Marcos Eduardo
Delcourt, Stella Maris
Testa, Roberto
Gogorza, Sebastian J.
Argibay, Pablo
author Fagundez, Carol Betiana
author_facet Fagundez, Carol Betiana
Loresi, Monica Alejandra
Ojea Quintana, Marcos Eduardo
Delcourt, Stella Maris
Testa, Roberto
Gogorza, Sebastian J.
Argibay, Pablo
author_role author
author2 Loresi, Monica Alejandra
Ojea Quintana, Marcos Eduardo
Delcourt, Stella Maris
Testa, Roberto
Gogorza, Sebastian J.
Argibay, Pablo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EMBRYONIC STEM CELLS
HATCHED BLASTOCYSTS
EMBRYOID BODY FORMATION
C57BL/6 MOUSE STRAIN
topic EMBRYONIC STEM CELLS
HATCHED BLASTOCYSTS
EMBRYOID BODY FORMATION
C57BL/6 MOUSE STRAIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present study, we derived stem cells from hatched blastocyst-stage mouse embryos from C57BL/6 strain employing a knockout serum replacement instead of the traditional foetal calf serum, avoiding the use of immunosurgery. Although foetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The cells derived were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage specific embryonic antigen 1 (SSEA-1) and the octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes was better compared with hanging drop method for differentiation by means of embryoid body formation. The mouse embryonic stem cells obtained during this work showed spontaneous differentiation to derivatives of the three germ layers in culture media supplemented with foetal calf serum but not with knockout serum.
Fil: Fagundez, Carol Betiana. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ojea Quintana, Marcos Eduardo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Delcourt, Stella Maris. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Testa, Roberto. Hospital Italiano; Argentina
Fil: Gogorza, Sebastian J.. Hospital Italiano; Argentina
Fil: Argibay, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
description In the present study, we derived stem cells from hatched blastocyst-stage mouse embryos from C57BL/6 strain employing a knockout serum replacement instead of the traditional foetal calf serum, avoiding the use of immunosurgery. Although foetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The cells derived were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage specific embryonic antigen 1 (SSEA-1) and the octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes was better compared with hanging drop method for differentiation by means of embryoid body formation. The mouse embryonic stem cells obtained during this work showed spontaneous differentiation to derivatives of the three germ layers in culture media supplemented with foetal calf serum but not with knockout serum.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/248042
Fagundez, Carol Betiana; Loresi, Monica Alejandra; Ojea Quintana, Marcos Eduardo; Delcourt, Stella Maris; Testa, Roberto; et al.; A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation; Academic Press Ltd - Elsevier Science Ltd; Cell Biology International; 33; 11; 1-2013; 1196-1200
1065-6995
CONICET Digital
CONICET
url http://hdl.handle.net/11336/248042
identifier_str_mv Fagundez, Carol Betiana; Loresi, Monica Alejandra; Ojea Quintana, Marcos Eduardo; Delcourt, Stella Maris; Testa, Roberto; et al.; A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation; Academic Press Ltd - Elsevier Science Ltd; Cell Biology International; 33; 11; 1-2013; 1196-1200
1065-6995
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1065699509001954
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellbi.2009.08.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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