Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study

Autores
Pincelli, Michelina Marta; Levstein, Patricia Rebeca; Fidelio, Gerardo Daniel; Gennaro, Ana Maria
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of cholesterol (Chol) on two kinds of glycolipid assemblies, one composed of monosialogangliosides (G(M1a)) and the other formed by a natural mixture of bovine brain gangliosides (TBG), has been analysed. The experimental approach involves spin label electron paramagnetic resonance (EPR) in aqueous lipid dispersions. The employment of a hydrosoluble spin label and a `quencher´ of the EPR signal that is not able to permeate lipid interfaces, allowed us to conclude that G(M1a)/Chol mixtures give rise to vesicles at Chol proportions for which TBG/Chol mixtures form micelles. The use of different liposoluble spin labels reveals that cholesterol produces a straightening of the hydrocarbon chains in both lipid systems. In GM1a/Chol mixtures, this feature is more pronounced and it is coupled with a decrease in polarity at the chain ends.
Fil: Pincelli, Michelina Marta. Universidad Nacional de Córdoba; Argentina
Fil: Levstein, Patricia Rebeca. Universidad Nacional de Córdoba; Argentina
Fil: Fidelio, Gerardo Daniel. Universidad Nacional de Córdoba; Argentina
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Materia
Glycolipids
Lipid Polymorphism
Supramolecular Aggregates
Micelles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27676
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cholesterol-induced alterations of the packing properties of gangliosides: an EPR studyPincelli, Michelina MartaLevstein, Patricia RebecaFidelio, Gerardo DanielGennaro, Ana MariaGlycolipidsLipid PolymorphismSupramolecular AggregatesMicelleshttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The effect of cholesterol (Chol) on two kinds of glycolipid assemblies, one composed of monosialogangliosides (G(M1a)) and the other formed by a natural mixture of bovine brain gangliosides (TBG), has been analysed. The experimental approach involves spin label electron paramagnetic resonance (EPR) in aqueous lipid dispersions. The employment of a hydrosoluble spin label and a `quencher´ of the EPR signal that is not able to permeate lipid interfaces, allowed us to conclude that G(M1a)/Chol mixtures give rise to vesicles at Chol proportions for which TBG/Chol mixtures form micelles. The use of different liposoluble spin labels reveals that cholesterol produces a straightening of the hydrocarbon chains in both lipid systems. In GM1a/Chol mixtures, this feature is more pronounced and it is coupled with a decrease in polarity at the chain ends.Fil: Pincelli, Michelina Marta. Universidad Nacional de Córdoba; ArgentinaFil: Levstein, Patricia Rebeca. Universidad Nacional de Córdoba; ArgentinaFil: Fidelio, Gerardo Daniel. Universidad Nacional de Córdoba; ArgentinaFil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaElsevier Ireland2000-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27676Pincelli, Michelina Marta; Levstein, Patricia Rebeca; Fidelio, Gerardo Daniel; Gennaro, Ana Maria; Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study; Elsevier Ireland; Chemistry And Physics Of Lipids; 104; 2; 2-2000; 193-2060009-3084CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0009-3084(99)00127-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:32:39Zoai:ri.conicet.gov.ar:11336/27676instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:32:39.594CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study
title Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study
spellingShingle Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study
Pincelli, Michelina Marta
Glycolipids
Lipid Polymorphism
Supramolecular Aggregates
Micelles
title_short Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study
title_full Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study
title_fullStr Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study
title_full_unstemmed Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study
title_sort Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study
dc.creator.none.fl_str_mv Pincelli, Michelina Marta
Levstein, Patricia Rebeca
Fidelio, Gerardo Daniel
Gennaro, Ana Maria
author Pincelli, Michelina Marta
author_facet Pincelli, Michelina Marta
Levstein, Patricia Rebeca
Fidelio, Gerardo Daniel
Gennaro, Ana Maria
author_role author
author2 Levstein, Patricia Rebeca
Fidelio, Gerardo Daniel
Gennaro, Ana Maria
author2_role author
author
author
dc.subject.none.fl_str_mv Glycolipids
Lipid Polymorphism
Supramolecular Aggregates
Micelles
topic Glycolipids
Lipid Polymorphism
Supramolecular Aggregates
Micelles
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effect of cholesterol (Chol) on two kinds of glycolipid assemblies, one composed of monosialogangliosides (G(M1a)) and the other formed by a natural mixture of bovine brain gangliosides (TBG), has been analysed. The experimental approach involves spin label electron paramagnetic resonance (EPR) in aqueous lipid dispersions. The employment of a hydrosoluble spin label and a `quencher´ of the EPR signal that is not able to permeate lipid interfaces, allowed us to conclude that G(M1a)/Chol mixtures give rise to vesicles at Chol proportions for which TBG/Chol mixtures form micelles. The use of different liposoluble spin labels reveals that cholesterol produces a straightening of the hydrocarbon chains in both lipid systems. In GM1a/Chol mixtures, this feature is more pronounced and it is coupled with a decrease in polarity at the chain ends.
Fil: Pincelli, Michelina Marta. Universidad Nacional de Córdoba; Argentina
Fil: Levstein, Patricia Rebeca. Universidad Nacional de Córdoba; Argentina
Fil: Fidelio, Gerardo Daniel. Universidad Nacional de Córdoba; Argentina
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
description The effect of cholesterol (Chol) on two kinds of glycolipid assemblies, one composed of monosialogangliosides (G(M1a)) and the other formed by a natural mixture of bovine brain gangliosides (TBG), has been analysed. The experimental approach involves spin label electron paramagnetic resonance (EPR) in aqueous lipid dispersions. The employment of a hydrosoluble spin label and a `quencher´ of the EPR signal that is not able to permeate lipid interfaces, allowed us to conclude that G(M1a)/Chol mixtures give rise to vesicles at Chol proportions for which TBG/Chol mixtures form micelles. The use of different liposoluble spin labels reveals that cholesterol produces a straightening of the hydrocarbon chains in both lipid systems. In GM1a/Chol mixtures, this feature is more pronounced and it is coupled with a decrease in polarity at the chain ends.
publishDate 2000
dc.date.none.fl_str_mv 2000-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27676
Pincelli, Michelina Marta; Levstein, Patricia Rebeca; Fidelio, Gerardo Daniel; Gennaro, Ana Maria; Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study; Elsevier Ireland; Chemistry And Physics Of Lipids; 104; 2; 2-2000; 193-206
0009-3084
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27676
identifier_str_mv Pincelli, Michelina Marta; Levstein, Patricia Rebeca; Fidelio, Gerardo Daniel; Gennaro, Ana Maria; Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study; Elsevier Ireland; Chemistry And Physics Of Lipids; 104; 2; 2-2000; 193-206
0009-3084
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0009-3084(99)00127-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1858306154762862592
score 12.665996

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