Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation

Autores
Pescio, Lucila Gisele; Santacreu, Bruno Jaime; Lopez, Vanina Gisela; Pavan, Carlos Humberto; Romero, Daniela Judith; Favale, Nicolas Octavio; Sterin, Norma Beatriz
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ceramides and complex sphingolipids with defined acyl-chain lengths play important roles in numerous cell processes. Six ceramide synthase isoenzymes (CerS1-6) are the key enzymes responsible for the production of the diversity of molecular species. In this study, we investigated the changes in sphingolipid metabolism during the differentiation of Madin-Darby Canine Kidney (MDCK) cells. By MALDI TOF TOF MS, we analyzed the molecular species of ceramide (Cer), glucosylceramide (GlcCer), lactosylceramide (LacCer) and sphingomyelin (SM) in non-differentiated and differentiated cells (cultured under hypertonicity). The molecular species detected were the same, but cells subjected to hypertonicity presented higher levels of C24:1 Cer, C24:1 GlcCer, C24:1 SM and C16:0 LacCer. Consistently with the molecular species, MDCK cells expressed CerS2, CerS4 and CerS6, but with no differences during cell differentiation. We next evaluated the different synthesis pathways with sphingolipid inhibitors and found that cells subjected to hypertonicity in the presence of amitriptyline, an inhibitor of acid sphingomyelinase, showed decreased radiolabeled incorporation in LacCer and cells did not develop a mature apical membrane. These results suggest that hypertonicity induces the endolysosomal degradation of SM, generating the Cer used as substrate for the synthesis of specific molecular species of glycosphingolipids that are essential for MDCK cell differentiation.
Fil: Pescio, Lucila Gisele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Lopez, Vanina Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Pavan, Carlos Humberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Romero, Daniela Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Sterin, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Materia
Ceramides
Glycolipids
Kidney
Cell Differentiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47589
Acceder
id CONICETDig_24dcc6cf3799aaf901dff974294a0266
oai_identifier_str oai:ri.conicet.gov.ar:11336/47589
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiationPescio, Lucila GiseleSantacreu, Bruno JaimeLopez, Vanina GiselaPavan, Carlos HumbertoRomero, Daniela JudithFavale, Nicolas OctavioSterin, Norma BeatrizCeramidesGlycolipidsKidneyCell Differentiationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ceramides and complex sphingolipids with defined acyl-chain lengths play important roles in numerous cell processes. Six ceramide synthase isoenzymes (CerS1-6) are the key enzymes responsible for the production of the diversity of molecular species. In this study, we investigated the changes in sphingolipid metabolism during the differentiation of Madin-Darby Canine Kidney (MDCK) cells. By MALDI TOF TOF MS, we analyzed the molecular species of ceramide (Cer), glucosylceramide (GlcCer), lactosylceramide (LacCer) and sphingomyelin (SM) in non-differentiated and differentiated cells (cultured under hypertonicity). The molecular species detected were the same, but cells subjected to hypertonicity presented higher levels of C24:1 Cer, C24:1 GlcCer, C24:1 SM and C16:0 LacCer. Consistently with the molecular species, MDCK cells expressed CerS2, CerS4 and CerS6, but with no differences during cell differentiation. We next evaluated the different synthesis pathways with sphingolipid inhibitors and found that cells subjected to hypertonicity in the presence of amitriptyline, an inhibitor of acid sphingomyelinase, showed decreased radiolabeled incorporation in LacCer and cells did not develop a mature apical membrane. These results suggest that hypertonicity induces the endolysosomal degradation of SM, generating the Cer used as substrate for the synthesis of specific molecular species of glycosphingolipids that are essential for MDCK cell differentiation.Fil: Pescio, Lucila Gisele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; ArgentinaFil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; ArgentinaFil: Lopez, Vanina Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; ArgentinaFil: Pavan, Carlos Humberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; ArgentinaFil: Romero, Daniela Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; ArgentinaFil: Sterin, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaAmerican Society for Biochemistry and Molecular Biology2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47589Pescio, Lucila Gisele; Santacreu, Bruno Jaime; Lopez, Vanina Gisela; Pavan, Carlos Humberto; Romero, Daniela Judith; et al.; Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 58; 7; 7-2017; 1428-14380022-22751539-7262CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/lookup/doi/10.1194/jlr.M076349info:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.M076349info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:12Zoai:ri.conicet.gov.ar:11336/47589instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:12.623CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation
title Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation
spellingShingle Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation
Pescio, Lucila Gisele
Ceramides
Glycolipids
Kidney
Cell Differentiation
title_short Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation
title_full Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation
title_fullStr Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation
title_full_unstemmed Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation
title_sort Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation
dc.creator.none.fl_str_mv Pescio, Lucila Gisele
Santacreu, Bruno Jaime
Lopez, Vanina Gisela
Pavan, Carlos Humberto
Romero, Daniela Judith
Favale, Nicolas Octavio
Sterin, Norma Beatriz
author Pescio, Lucila Gisele
author_facet Pescio, Lucila Gisele
Santacreu, Bruno Jaime
Lopez, Vanina Gisela
Pavan, Carlos Humberto
Romero, Daniela Judith
Favale, Nicolas Octavio
Sterin, Norma Beatriz
author_role author
author2 Santacreu, Bruno Jaime
Lopez, Vanina Gisela
Pavan, Carlos Humberto
Romero, Daniela Judith
Favale, Nicolas Octavio
Sterin, Norma Beatriz
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ceramides
Glycolipids
Kidney
Cell Differentiation
topic Ceramides
Glycolipids
Kidney
Cell Differentiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ceramides and complex sphingolipids with defined acyl-chain lengths play important roles in numerous cell processes. Six ceramide synthase isoenzymes (CerS1-6) are the key enzymes responsible for the production of the diversity of molecular species. In this study, we investigated the changes in sphingolipid metabolism during the differentiation of Madin-Darby Canine Kidney (MDCK) cells. By MALDI TOF TOF MS, we analyzed the molecular species of ceramide (Cer), glucosylceramide (GlcCer), lactosylceramide (LacCer) and sphingomyelin (SM) in non-differentiated and differentiated cells (cultured under hypertonicity). The molecular species detected were the same, but cells subjected to hypertonicity presented higher levels of C24:1 Cer, C24:1 GlcCer, C24:1 SM and C16:0 LacCer. Consistently with the molecular species, MDCK cells expressed CerS2, CerS4 and CerS6, but with no differences during cell differentiation. We next evaluated the different synthesis pathways with sphingolipid inhibitors and found that cells subjected to hypertonicity in the presence of amitriptyline, an inhibitor of acid sphingomyelinase, showed decreased radiolabeled incorporation in LacCer and cells did not develop a mature apical membrane. These results suggest that hypertonicity induces the endolysosomal degradation of SM, generating the Cer used as substrate for the synthesis of specific molecular species of glycosphingolipids that are essential for MDCK cell differentiation.
Fil: Pescio, Lucila Gisele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Lopez, Vanina Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Pavan, Carlos Humberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Romero, Daniela Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Sterin, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
description Ceramides and complex sphingolipids with defined acyl-chain lengths play important roles in numerous cell processes. Six ceramide synthase isoenzymes (CerS1-6) are the key enzymes responsible for the production of the diversity of molecular species. In this study, we investigated the changes in sphingolipid metabolism during the differentiation of Madin-Darby Canine Kidney (MDCK) cells. By MALDI TOF TOF MS, we analyzed the molecular species of ceramide (Cer), glucosylceramide (GlcCer), lactosylceramide (LacCer) and sphingomyelin (SM) in non-differentiated and differentiated cells (cultured under hypertonicity). The molecular species detected were the same, but cells subjected to hypertonicity presented higher levels of C24:1 Cer, C24:1 GlcCer, C24:1 SM and C16:0 LacCer. Consistently with the molecular species, MDCK cells expressed CerS2, CerS4 and CerS6, but with no differences during cell differentiation. We next evaluated the different synthesis pathways with sphingolipid inhibitors and found that cells subjected to hypertonicity in the presence of amitriptyline, an inhibitor of acid sphingomyelinase, showed decreased radiolabeled incorporation in LacCer and cells did not develop a mature apical membrane. These results suggest that hypertonicity induces the endolysosomal degradation of SM, generating the Cer used as substrate for the synthesis of specific molecular species of glycosphingolipids that are essential for MDCK cell differentiation.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47589
Pescio, Lucila Gisele; Santacreu, Bruno Jaime; Lopez, Vanina Gisela; Pavan, Carlos Humberto; Romero, Daniela Judith; et al.; Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 58; 7; 7-2017; 1428-1438
0022-2275
1539-7262
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47589
identifier_str_mv Pescio, Lucila Gisele; Santacreu, Bruno Jaime; Lopez, Vanina Gisela; Pavan, Carlos Humberto; Romero, Daniela Judith; et al.; Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 58; 7; 7-2017; 1428-1438
0022-2275
1539-7262
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/lookup/doi/10.1194/jlr.M076349
info:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.M076349
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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