Microalgae Lipid Characterization

Autores
Yao, Linxing; Gerde, Jose Arnaldo; Lee, Show Ling; Wang, Tong; Harrata, Kamel A.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To meet the growing interest of utilizing microalgae biomass in the production of biofuels and nutraceutical and pharmaceutical lipids, we need suitable analytical methods and a comprehensive database for their lipid components. The objective of the present work was to demonstrate methodology and provide data on fatty acid composition, lipid class content and composition, characteristics of the unsaponifiables, and type of chlorophylls of five microalgae. Microalgae lipids were fractionated into TAG, FFA, and polar lipids using TLC, and the composition of fatty acids in total lipids and in each lipid class, hydrocarbons, and sterols were determined by GC-MS. Glyco- and phospholipids were profiled by LC/ESI-MS. Chlorophylls and their related metabolites were qualified by LC/APCI-MS. The melting and crystallization profiles of microalgae total lipids and their esters were analyzed by DSC to evaluate their potential biofuel applications. Significant differences and complexities of lipid composition among the algae tested were observed. The compositional information is valuable for strain selection, downstream biomass fractionation, and utilization.
Fil: Yao, Linxing. Iowa State University; Estados Unidos
Fil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Produccion Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Lee, Show Ling. Iowa State University; Estados Unidos
Fil: Wang, Tong. Iowa State University; Estados Unidos
Fil: Harrata, Kamel A.. Iowa State University; Estados Unidos
Materia
Chlorophylls
Fatty Acids
Glycolipids
Hydrocarbons
Lipid Characterization
Microalgae
Sterols
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13429
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13429
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microalgae Lipid CharacterizationYao, LinxingGerde, Jose ArnaldoLee, Show LingWang, TongHarrata, Kamel A.ChlorophyllsFatty AcidsGlycolipidsHydrocarbonsLipid CharacterizationMicroalgaeSterolshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2To meet the growing interest of utilizing microalgae biomass in the production of biofuels and nutraceutical and pharmaceutical lipids, we need suitable analytical methods and a comprehensive database for their lipid components. The objective of the present work was to demonstrate methodology and provide data on fatty acid composition, lipid class content and composition, characteristics of the unsaponifiables, and type of chlorophylls of five microalgae. Microalgae lipids were fractionated into TAG, FFA, and polar lipids using TLC, and the composition of fatty acids in total lipids and in each lipid class, hydrocarbons, and sterols were determined by GC-MS. Glyco- and phospholipids were profiled by LC/ESI-MS. Chlorophylls and their related metabolites were qualified by LC/APCI-MS. The melting and crystallization profiles of microalgae total lipids and their esters were analyzed by DSC to evaluate their potential biofuel applications. Significant differences and complexities of lipid composition among the algae tested were observed. The compositional information is valuable for strain selection, downstream biomass fractionation, and utilization.Fil: Yao, Linxing. Iowa State University; Estados UnidosFil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Produccion Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Lee, Show Ling. Iowa State University; Estados UnidosFil: Wang, Tong. Iowa State University; Estados UnidosFil: Harrata, Kamel A.. Iowa State University; Estados UnidosAmerican Chemical Society2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13429Yao, Linxing; Gerde, Jose Arnaldo; Lee, Show Ling; Wang, Tong; Harrata, Kamel A.; Microalgae Lipid Characterization; American Chemical Society; Journal Of Agricultural And Food Chemistry; 63; 6; 1-2015; 1773-17870021-85611520-5118enginfo:eu-repo/semantics/altIdentifier/doi/10.1021/jf5050603info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/jf5050603info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:34Zoai:ri.conicet.gov.ar:11336/13429instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:35.066CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microalgae Lipid Characterization
title Microalgae Lipid Characterization
spellingShingle Microalgae Lipid Characterization
Yao, Linxing
Chlorophylls
Fatty Acids
Glycolipids
Hydrocarbons
Lipid Characterization
Microalgae
Sterols
title_short Microalgae Lipid Characterization
title_full Microalgae Lipid Characterization
title_fullStr Microalgae Lipid Characterization
title_full_unstemmed Microalgae Lipid Characterization
title_sort Microalgae Lipid Characterization
dc.creator.none.fl_str_mv Yao, Linxing
Gerde, Jose Arnaldo
Lee, Show Ling
Wang, Tong
Harrata, Kamel A.
author Yao, Linxing
author_facet Yao, Linxing
Gerde, Jose Arnaldo
Lee, Show Ling
Wang, Tong
Harrata, Kamel A.
author_role author
author2 Gerde, Jose Arnaldo
Lee, Show Ling
Wang, Tong
Harrata, Kamel A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Chlorophylls
Fatty Acids
Glycolipids
Hydrocarbons
Lipid Characterization
Microalgae
Sterols
topic Chlorophylls
Fatty Acids
Glycolipids
Hydrocarbons
Lipid Characterization
Microalgae
Sterols
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv To meet the growing interest of utilizing microalgae biomass in the production of biofuels and nutraceutical and pharmaceutical lipids, we need suitable analytical methods and a comprehensive database for their lipid components. The objective of the present work was to demonstrate methodology and provide data on fatty acid composition, lipid class content and composition, characteristics of the unsaponifiables, and type of chlorophylls of five microalgae. Microalgae lipids were fractionated into TAG, FFA, and polar lipids using TLC, and the composition of fatty acids in total lipids and in each lipid class, hydrocarbons, and sterols were determined by GC-MS. Glyco- and phospholipids were profiled by LC/ESI-MS. Chlorophylls and their related metabolites were qualified by LC/APCI-MS. The melting and crystallization profiles of microalgae total lipids and their esters were analyzed by DSC to evaluate their potential biofuel applications. Significant differences and complexities of lipid composition among the algae tested were observed. The compositional information is valuable for strain selection, downstream biomass fractionation, and utilization.
Fil: Yao, Linxing. Iowa State University; Estados Unidos
Fil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Produccion Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Lee, Show Ling. Iowa State University; Estados Unidos
Fil: Wang, Tong. Iowa State University; Estados Unidos
Fil: Harrata, Kamel A.. Iowa State University; Estados Unidos
description To meet the growing interest of utilizing microalgae biomass in the production of biofuels and nutraceutical and pharmaceutical lipids, we need suitable analytical methods and a comprehensive database for their lipid components. The objective of the present work was to demonstrate methodology and provide data on fatty acid composition, lipid class content and composition, characteristics of the unsaponifiables, and type of chlorophylls of five microalgae. Microalgae lipids were fractionated into TAG, FFA, and polar lipids using TLC, and the composition of fatty acids in total lipids and in each lipid class, hydrocarbons, and sterols were determined by GC-MS. Glyco- and phospholipids were profiled by LC/ESI-MS. Chlorophylls and their related metabolites were qualified by LC/APCI-MS. The melting and crystallization profiles of microalgae total lipids and their esters were analyzed by DSC to evaluate their potential biofuel applications. Significant differences and complexities of lipid composition among the algae tested were observed. The compositional information is valuable for strain selection, downstream biomass fractionation, and utilization.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13429
Yao, Linxing; Gerde, Jose Arnaldo; Lee, Show Ling; Wang, Tong; Harrata, Kamel A.; Microalgae Lipid Characterization; American Chemical Society; Journal Of Agricultural And Food Chemistry; 63; 6; 1-2015; 1773-1787
0021-8561
1520-5118
url http://hdl.handle.net/11336/13429
identifier_str_mv Yao, Linxing; Gerde, Jose Arnaldo; Lee, Show Ling; Wang, Tong; Harrata, Kamel A.; Microalgae Lipid Characterization; American Chemical Society; Journal Of Agricultural And Food Chemistry; 63; 6; 1-2015; 1773-1787
0021-8561
1520-5118
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/jf5050603
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/jf5050603
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269646851407872
score 13.13397

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