Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges

Autores
Pinazo, Maria Jesus; Forsyth, Colin J.; Lopez Albizu, Maria Constanza; Bisio, Margarita María Catalina; González Martínez, Adriana; Bohorquez, Laura C.; Santini, Maria Soledad; Altcheh, Jaime Marcelo; Alarcón de Noya, Belkisyolé; Sosa-Estani, Sergio Alejandro
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi infection is diagnosed by parasitological, molecular, and serological tests. Molecular methods based on DNA amplification provide a more sensitive alternative to classical parasitological techniques for detecting evidence of T. cruzi parasitemia, and are the preferred tests for congenital and oral transmission cases and parasite reactivation in chronically infected immunosuppressed individuals. In newborns at risk of vertical transmission, simplified diagnostic algorithms that provide timely results can reduce the high follow-up losses observed with current algorithms. Molecular methods have also proved useful for monitoring T. cruzi infection in solid organ transplantation recipients, regardless of host immune status, allowing parasite detection even before symptom manifestation. Furthermore, in the absence of other biomarkers and a practical test of cure, and given the limitations of serological methods, recent clinical guidelines have included polymerase chain reaction (PCR) to detect therapeutic failure after antiparasitic treatment in chronically infected adults. Increasing evidence supports the use of molecular tests in a clinical context, given the improved sensitivity and specificity of current assays – characteristics which largely depend on epidemiological factors and genetic and antigenic variability among T. cruzi strains. Further development and registration of commercial PCR kits will improve the use of molecular tests. We discuss the attributes of PCR and other molecular tests for clinical management in people with T. cruzi infection.
Fil: Pinazo, Maria Jesus. Dndi America Latina;
Fil: Forsyth, Colin J.. Dndi America Latina;
Fil: Lopez Albizu, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Bisio, Margarita María Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: González Martínez, Adriana. Dndi America Latina;
Fil: Bohorquez, Laura C.. Dndi America Latina;
Fil: Santini, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Altcheh, Jaime Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires; Argentina
Fil: Alarcón de Noya, Belkisyolé. Universidad Central de Venezuela; Venezuela
Fil: Sosa-Estani, Sergio Alejandro. Dndi America Latina; . Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CHAGAS
TRYPANOSOMA CRUZI
INFECTIONS
DIAGNOSTIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/220025
Acceder
id CONICETDig_7e8d8146e4e737de7490959383dbd796
oai_identifier_str oai:ri.conicet.gov.ar:11336/220025
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challengesPinazo, Maria JesusForsyth, Colin J.Lopez Albizu, Maria ConstanzaBisio, Margarita María CatalinaGonzález Martínez, AdrianaBohorquez, Laura C.Santini, Maria SoledadAltcheh, Jaime MarceloAlarcón de Noya, BelkisyoléSosa-Estani, Sergio AlejandroCHAGASTRYPANOSOMA CRUZIINFECTIONSDIAGNOSTIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi infection is diagnosed by parasitological, molecular, and serological tests. Molecular methods based on DNA amplification provide a more sensitive alternative to classical parasitological techniques for detecting evidence of T. cruzi parasitemia, and are the preferred tests for congenital and oral transmission cases and parasite reactivation in chronically infected immunosuppressed individuals. In newborns at risk of vertical transmission, simplified diagnostic algorithms that provide timely results can reduce the high follow-up losses observed with current algorithms. Molecular methods have also proved useful for monitoring T. cruzi infection in solid organ transplantation recipients, regardless of host immune status, allowing parasite detection even before symptom manifestation. Furthermore, in the absence of other biomarkers and a practical test of cure, and given the limitations of serological methods, recent clinical guidelines have included polymerase chain reaction (PCR) to detect therapeutic failure after antiparasitic treatment in chronically infected adults. Increasing evidence supports the use of molecular tests in a clinical context, given the improved sensitivity and specificity of current assays – characteristics which largely depend on epidemiological factors and genetic and antigenic variability among T. cruzi strains. Further development and registration of commercial PCR kits will improve the use of molecular tests. We discuss the attributes of PCR and other molecular tests for clinical management in people with T. cruzi infection.Fil: Pinazo, Maria Jesus. Dndi America Latina;Fil: Forsyth, Colin J.. Dndi America Latina;Fil: Lopez Albizu, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Bisio, Margarita María Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: González Martínez, Adriana. Dndi America Latina;Fil: Bohorquez, Laura C.. Dndi America Latina;Fil: Santini, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Altcheh, Jaime Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires; ArgentinaFil: Alarcón de Noya, Belkisyolé. Universidad Central de Venezuela; VenezuelaFil: Sosa-Estani, Sergio Alejandro. Dndi America Latina; . Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFrontiers Media2023-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/220025Pinazo, Maria Jesus; Forsyth, Colin J.; Lopez Albizu, Maria Constanza; Bisio, Margarita María Catalina; González Martínez, Adriana; et al.; Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges; Frontiers Media; Frontiers in parasitology; 11-2023; 1-82813-2424CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpara.2023.1241154info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpara.2023.1241154/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:46:04Zoai:ri.conicet.gov.ar:11336/220025instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:46:04.631CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges
title Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges
spellingShingle Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges
Pinazo, Maria Jesus
CHAGAS
TRYPANOSOMA CRUZI
INFECTIONS
DIAGNOSTIC
title_short Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges
title_full Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges
title_fullStr Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges
title_full_unstemmed Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges
title_sort Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges
dc.creator.none.fl_str_mv Pinazo, Maria Jesus
Forsyth, Colin J.
Lopez Albizu, Maria Constanza
Bisio, Margarita María Catalina
González Martínez, Adriana
Bohorquez, Laura C.
Santini, Maria Soledad
Altcheh, Jaime Marcelo
Alarcón de Noya, Belkisyolé
Sosa-Estani, Sergio Alejandro
author Pinazo, Maria Jesus
author_facet Pinazo, Maria Jesus
Forsyth, Colin J.
Lopez Albizu, Maria Constanza
Bisio, Margarita María Catalina
González Martínez, Adriana
Bohorquez, Laura C.
Santini, Maria Soledad
Altcheh, Jaime Marcelo
Alarcón de Noya, Belkisyolé
Sosa-Estani, Sergio Alejandro
author_role author
author2 Forsyth, Colin J.
Lopez Albizu, Maria Constanza
Bisio, Margarita María Catalina
González Martínez, Adriana
Bohorquez, Laura C.
Santini, Maria Soledad
Altcheh, Jaime Marcelo
Alarcón de Noya, Belkisyolé
Sosa-Estani, Sergio Alejandro
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHAGAS
TRYPANOSOMA CRUZI
INFECTIONS
DIAGNOSTIC
topic CHAGAS
TRYPANOSOMA CRUZI
INFECTIONS
DIAGNOSTIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi infection is diagnosed by parasitological, molecular, and serological tests. Molecular methods based on DNA amplification provide a more sensitive alternative to classical parasitological techniques for detecting evidence of T. cruzi parasitemia, and are the preferred tests for congenital and oral transmission cases and parasite reactivation in chronically infected immunosuppressed individuals. In newborns at risk of vertical transmission, simplified diagnostic algorithms that provide timely results can reduce the high follow-up losses observed with current algorithms. Molecular methods have also proved useful for monitoring T. cruzi infection in solid organ transplantation recipients, regardless of host immune status, allowing parasite detection even before symptom manifestation. Furthermore, in the absence of other biomarkers and a practical test of cure, and given the limitations of serological methods, recent clinical guidelines have included polymerase chain reaction (PCR) to detect therapeutic failure after antiparasitic treatment in chronically infected adults. Increasing evidence supports the use of molecular tests in a clinical context, given the improved sensitivity and specificity of current assays – characteristics which largely depend on epidemiological factors and genetic and antigenic variability among T. cruzi strains. Further development and registration of commercial PCR kits will improve the use of molecular tests. We discuss the attributes of PCR and other molecular tests for clinical management in people with T. cruzi infection.
Fil: Pinazo, Maria Jesus. Dndi America Latina;
Fil: Forsyth, Colin J.. Dndi America Latina;
Fil: Lopez Albizu, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Bisio, Margarita María Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: González Martínez, Adriana. Dndi America Latina;
Fil: Bohorquez, Laura C.. Dndi America Latina;
Fil: Santini, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Altcheh, Jaime Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires; Argentina
Fil: Alarcón de Noya, Belkisyolé. Universidad Central de Venezuela; Venezuela
Fil: Sosa-Estani, Sergio Alejandro. Dndi America Latina; . Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Trypanosoma cruzi infection is diagnosed by parasitological, molecular, and serological tests. Molecular methods based on DNA amplification provide a more sensitive alternative to classical parasitological techniques for detecting evidence of T. cruzi parasitemia, and are the preferred tests for congenital and oral transmission cases and parasite reactivation in chronically infected immunosuppressed individuals. In newborns at risk of vertical transmission, simplified diagnostic algorithms that provide timely results can reduce the high follow-up losses observed with current algorithms. Molecular methods have also proved useful for monitoring T. cruzi infection in solid organ transplantation recipients, regardless of host immune status, allowing parasite detection even before symptom manifestation. Furthermore, in the absence of other biomarkers and a practical test of cure, and given the limitations of serological methods, recent clinical guidelines have included polymerase chain reaction (PCR) to detect therapeutic failure after antiparasitic treatment in chronically infected adults. Increasing evidence supports the use of molecular tests in a clinical context, given the improved sensitivity and specificity of current assays – characteristics which largely depend on epidemiological factors and genetic and antigenic variability among T. cruzi strains. Further development and registration of commercial PCR kits will improve the use of molecular tests. We discuss the attributes of PCR and other molecular tests for clinical management in people with T. cruzi infection.
publishDate 2023
dc.date.none.fl_str_mv 2023-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/220025
Pinazo, Maria Jesus; Forsyth, Colin J.; Lopez Albizu, Maria Constanza; Bisio, Margarita María Catalina; González Martínez, Adriana; et al.; Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges; Frontiers Media; Frontiers in parasitology; 11-2023; 1-8
2813-2424
CONICET Digital
CONICET
url http://hdl.handle.net/11336/220025
identifier_str_mv Pinazo, Maria Jesus; Forsyth, Colin J.; Lopez Albizu, Maria Constanza; Bisio, Margarita María Catalina; González Martínez, Adriana; et al.; Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges; Frontiers Media; Frontiers in parasitology; 11-2023; 1-8
2813-2424
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fpara.2023.1241154
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpara.2023.1241154/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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