Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels

Autores
Ferro, Yannis; Perullini, Ana Mercedes; Jobbagy, Matias; Aldabe, Sara Alfonsina; Durrieu, Claude
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure.
Fil: Ferro, Yannis. Université Claude Bernard Lyon 1; Francia
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Jobbagy, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Aldabe, Sara Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Durrieu, Claude. Université Claude Bernard Lyon 1; Francia
Materia
Biosensor
Algae
Sol-gel
Herbicides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275113
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica HydrogelsFerro, YannisPerullini, Ana MercedesJobbagy, MatiasAldabe, Sara AlfonsinaDurrieu, ClaudeBiosensorAlgaeSol-gelHerbicideshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure.Fil: Ferro, Yannis. Université Claude Bernard Lyon 1; FranciaFil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Jobbagy, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Aldabe, Sara Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Durrieu, Claude. Université Claude Bernard Lyon 1; FranciaMolecular Diversity Preservation International2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275113Ferro, Yannis; Perullini, Ana Mercedes; Jobbagy, Matias; Aldabe, Sara Alfonsina; Durrieu, Claude; Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels; Molecular Diversity Preservation International; Sensors; 12; 12; 12-2012; 16879-168911424-8220CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-8220/12/12/16879info:eu-repo/semantics/altIdentifier/doi/10.3390/s121216879info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T09:02:55Zoai:ri.conicet.gov.ar:11336/275113instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 09:02:55.358CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
spellingShingle Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
Ferro, Yannis
Biosensor
Algae
Sol-gel
Herbicides
title_short Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title_full Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title_fullStr Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title_full_unstemmed Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
title_sort Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
dc.creator.none.fl_str_mv Ferro, Yannis
Perullini, Ana Mercedes
Jobbagy, Matias
Aldabe, Sara Alfonsina
Durrieu, Claude
author Ferro, Yannis
author_facet Ferro, Yannis
Perullini, Ana Mercedes
Jobbagy, Matias
Aldabe, Sara Alfonsina
Durrieu, Claude
author_role author
author2 Perullini, Ana Mercedes
Jobbagy, Matias
Aldabe, Sara Alfonsina
Durrieu, Claude
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biosensor
Algae
Sol-gel
Herbicides
topic Biosensor
Algae
Sol-gel
Herbicides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure.
Fil: Ferro, Yannis. Université Claude Bernard Lyon 1; Francia
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Jobbagy, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Aldabe, Sara Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Durrieu, Claude. Université Claude Bernard Lyon 1; Francia
description A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275113
Ferro, Yannis; Perullini, Ana Mercedes; Jobbagy, Matias; Aldabe, Sara Alfonsina; Durrieu, Claude; Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels; Molecular Diversity Preservation International; Sensors; 12; 12; 12-2012; 16879-16891
1424-8220
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275113
identifier_str_mv Ferro, Yannis; Perullini, Ana Mercedes; Jobbagy, Matias; Aldabe, Sara Alfonsina; Durrieu, Claude; Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels; Molecular Diversity Preservation International; Sensors; 12; 12; 12-2012; 16879-16891
1424-8220
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-8220/12/12/16879
info:eu-repo/semantics/altIdentifier/doi/10.3390/s121216879
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1850505230564720640
score 13.214268

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