Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels

Autores
Ferro, Y.; Perullini, M.; Jobbagy, M.; Bilmes, S.A.; Durrieu, C.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Jobbagy, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bilmes, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Sensors 2012;12(12):16879-16891
Materia
Algae
Atrazine
Biosensor
Chlorophyll fluorescence
DCMU
Herbicides
Sol-gel
Aquatic environments
Chlamydomonas reinhardtii
Chlorophyll fluorescence
DCMU
Environmental Monitoring
Fluorescence enhancement
Pseudokirchneriella subcapitata
Two-step procedure
Algae
Herbicides
Hydrogels
Microorganisms
Silica
Sol-gels
Weed control
Biosensors
atrazine
diuron
fresh water
article
chemistry
Chlamydomonas reinhardtii
Chlorella vulgaris
environmental monitoring
equipment
genetic procedures
human
hydrogel
isolation and purification
microalga
water pollutant
Atrazine
Biosensing Techniques
Chlamydomonas reinhardtii
Chlorella vulgaris
Diuron
Environmental Monitoring
Fresh Water
Humans
Hydrogel
Microalgae
Water Pollutants, Chemical
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_14248220_v12_n12_p16879_Ferro
Acceder
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oai_identifier_str paperaa:paper_14248220_v12_n12_p16879_Ferro
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogelsFerro, Y.Perullini, M.Jobbagy, M.Bilmes, S.A.Durrieu, C.AlgaeAtrazineBiosensorChlorophyll fluorescenceDCMUHerbicidesSol-gelAquatic environmentsChlamydomonas reinhardtiiChlorophyll fluorescenceDCMUEnvironmental MonitoringFluorescence enhancementPseudokirchneriella subcapitataTwo-step procedureAlgaeHerbicidesHydrogelsMicroorganismsSilicaSol-gelsWeed controlBiosensorsatrazinediuronfresh waterarticlechemistryChlamydomonas reinhardtiiChlorella vulgarisenvironmental monitoringequipmentgenetic procedureshumanhydrogelisolation and purificationmicroalgawater pollutantAtrazineBiosensing TechniquesChlamydomonas reinhardtiiChlorella vulgarisDiuronEnvironmental MonitoringFresh WaterHumansHydrogelMicroalgaeWater Pollutants, ChemicalA new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland.Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Jobbagy, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Bilmes, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_FerroSensors 2012;12(12):16879-16891reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-12-04T09:22:39Zpaperaa:paper_14248220_v12_n12_p16879_FerroInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-12-04 09:22:41.334Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
spellingShingle Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
Ferro, Y.
Algae
Atrazine
Biosensor
Chlorophyll fluorescence
DCMU
Herbicides
Sol-gel
Aquatic environments
Chlamydomonas reinhardtii
Chlorophyll fluorescence
DCMU
Environmental Monitoring
Fluorescence enhancement
Pseudokirchneriella subcapitata
Two-step procedure
Algae
Herbicides
Hydrogels
Microorganisms
Silica
Sol-gels
Weed control
Biosensors
atrazine
diuron
fresh water
article
chemistry
Chlamydomonas reinhardtii
Chlorella vulgaris
environmental monitoring
equipment
genetic procedures
human
hydrogel
isolation and purification
microalga
water pollutant
Atrazine
Biosensing Techniques
Chlamydomonas reinhardtii
Chlorella vulgaris
Diuron
Environmental Monitoring
Fresh Water
Humans
Hydrogel
Microalgae
Water Pollutants, Chemical
title_short Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title_full Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title_fullStr Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title_full_unstemmed Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
title_sort Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
dc.creator.none.fl_str_mv Ferro, Y.
Perullini, M.
Jobbagy, M.
Bilmes, S.A.
Durrieu, C.
author Ferro, Y.
author_facet Ferro, Y.
Perullini, M.
Jobbagy, M.
Bilmes, S.A.
Durrieu, C.
author_role author
author2 Perullini, M.
Jobbagy, M.
Bilmes, S.A.
Durrieu, C.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Algae
Atrazine
Biosensor
Chlorophyll fluorescence
DCMU
Herbicides
Sol-gel
Aquatic environments
Chlamydomonas reinhardtii
Chlorophyll fluorescence
DCMU
Environmental Monitoring
Fluorescence enhancement
Pseudokirchneriella subcapitata
Two-step procedure
Algae
Herbicides
Hydrogels
Microorganisms
Silica
Sol-gels
Weed control
Biosensors
atrazine
diuron
fresh water
article
chemistry
Chlamydomonas reinhardtii
Chlorella vulgaris
environmental monitoring
equipment
genetic procedures
human
hydrogel
isolation and purification
microalga
water pollutant
Atrazine
Biosensing Techniques
Chlamydomonas reinhardtii
Chlorella vulgaris
Diuron
Environmental Monitoring
Fresh Water
Humans
Hydrogel
Microalgae
Water Pollutants, Chemical
topic Algae
Atrazine
Biosensor
Chlorophyll fluorescence
DCMU
Herbicides
Sol-gel
Aquatic environments
Chlamydomonas reinhardtii
Chlorophyll fluorescence
DCMU
Environmental Monitoring
Fluorescence enhancement
Pseudokirchneriella subcapitata
Two-step procedure
Algae
Herbicides
Hydrogels
Microorganisms
Silica
Sol-gels
Weed control
Biosensors
atrazine
diuron
fresh water
article
chemistry
Chlamydomonas reinhardtii
Chlorella vulgaris
environmental monitoring
equipment
genetic procedures
human
hydrogel
isolation and purification
microalga
water pollutant
Atrazine
Biosensing Techniques
Chlamydomonas reinhardtii
Chlorella vulgaris
Diuron
Environmental Monitoring
Fresh Water
Humans
Hydrogel
Microalgae
Water Pollutants, Chemical
dc.description.none.fl_txt_mv A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Jobbagy, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bilmes, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_Ferro
url http://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_Ferro
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Sensors 2012;12(12):16879-16891
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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