Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale

Autores
Moore, Geromy G.; Elliott, Jacalyn L.; Singh, Rakhi; Horn, Bruce W.; Dorner, Jeo W.; Stone, Eric A.; Chulze, Sofia Noemi; Barros, Germán Gustavo; Naik, Manjunath K.; Wright, Graeme C.; Hell, Kerstin; Carbone, Ignazio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aflatoxins are produced by Aspergillus flavus and A. parasiticus in oil-rich seed and grain crops and are a serious problem in agriculture, with aflatoxin B1 being the most carcinogenic natural compound known. Sexual reproduction in these species occurs between individuals belonging to different vegetative compatibility groups (VCGs). We examined natural genetic variation in 758 isolates of A. flavus, A. parasiticus and A. minisclerotigenes sampled from single peanut fields in the United States (Georgia), Africa (Benin), Argentina (Co´rdoba), Australia (Queensland) and India (Karnataka). Analysis of DNA sequence variation across multiple intergenic regions in the aflatoxin gene clusters of A. flavus, A. parasiticus and A. minisclerotigenes revealed significant linkage disequilibrium (LD) organized into distinct blocks that are conserved across different localities, suggesting that genetic recombination is nonrandom and a global occurrence. To assess the contributions of asexual and sexual reproduction to fixation and maintenance of toxin chemotype diversity in populations from each locality/species, we tested the null hypothesis of an equal number of MAT1-1 and MAT1-2 mating-type individuals, which is indicative of a sexually recombining population. All samples were clone-corrected using multi-locus sequence typing which associates closely with VCG. For both A. flavus and A. parasiticus, when the proportions of MAT1-1 and MAT1-2 were significantly different, there was more extensive LD in the aflatoxin cluster and populations were fixed for specific toxin chemotype classes, either the non-aflatoxigenic class in A. flavus or the B1-dominant and G1-dominant classes in A. parasiticus. A mating type ratio close to 1:1 in A. flavus, A. parasiticus and A. minisclerotigenes was associated with higher recombination rates in the aflatoxin cluster and less pronounced chemotype differences in populations. This work shows that the reproductive nature of the population (more sexual versus more asexual) is predictive of aflatoxin chemotype diversity in these agriculturally important fungi.
Fil: Moore, Geromy G.. United States Department of Agriculture; Estados Unidos
Fil: Elliott, Jacalyn L.. University Of North Carolina; Estados Unidos
Fil: Singh, Rakhi. University Of North Carolina; Estados Unidos
Fil: Horn, Bruce W.. United States Department Of Agriculture; Estados Unidos
Fil: Dorner, Jeo W.. United States Department Of Agriculture; Estados Unidos
Fil: Stone, Eric A.. University Of North Carolina; Estados Unidos
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología E Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barros, Germán Gustavo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología E Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Naik, Manjunath K.. College of Agriculture. Department of Plant Pathology; India
Fil: Wright, Graeme C.. Department of Primary Industries; Australia
Fil: Hell, Kerstin. International Institute of Tropical Agriculture; Benín
Fil: Carbone, Ignazio. University Of North Carolina; Estados Unidos
Materia
Sexuality Diversity
Aspergillus
Aflatoxin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10182
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scaleMoore, Geromy G.Elliott, Jacalyn L.Singh, RakhiHorn, Bruce W.Dorner, Jeo W.Stone, Eric A.Chulze, Sofia NoemiBarros, Germán GustavoNaik, Manjunath K.Wright, Graeme C.Hell, KerstinCarbone, IgnazioSexuality DiversityAspergillusAflatoxinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aflatoxins are produced by Aspergillus flavus and A. parasiticus in oil-rich seed and grain crops and are a serious problem in agriculture, with aflatoxin B1 being the most carcinogenic natural compound known. Sexual reproduction in these species occurs between individuals belonging to different vegetative compatibility groups (VCGs). We examined natural genetic variation in 758 isolates of A. flavus, A. parasiticus and A. minisclerotigenes sampled from single peanut fields in the United States (Georgia), Africa (Benin), Argentina (Co´rdoba), Australia (Queensland) and India (Karnataka). Analysis of DNA sequence variation across multiple intergenic regions in the aflatoxin gene clusters of A. flavus, A. parasiticus and A. minisclerotigenes revealed significant linkage disequilibrium (LD) organized into distinct blocks that are conserved across different localities, suggesting that genetic recombination is nonrandom and a global occurrence. To assess the contributions of asexual and sexual reproduction to fixation and maintenance of toxin chemotype diversity in populations from each locality/species, we tested the null hypothesis of an equal number of MAT1-1 and MAT1-2 mating-type individuals, which is indicative of a sexually recombining population. All samples were clone-corrected using multi-locus sequence typing which associates closely with VCG. For both A. flavus and A. parasiticus, when the proportions of MAT1-1 and MAT1-2 were significantly different, there was more extensive LD in the aflatoxin cluster and populations were fixed for specific toxin chemotype classes, either the non-aflatoxigenic class in A. flavus or the B1-dominant and G1-dominant classes in A. parasiticus. A mating type ratio close to 1:1 in A. flavus, A. parasiticus and A. minisclerotigenes was associated with higher recombination rates in the aflatoxin cluster and less pronounced chemotype differences in populations. This work shows that the reproductive nature of the population (more sexual versus more asexual) is predictive of aflatoxin chemotype diversity in these agriculturally important fungi.Fil: Moore, Geromy G.. United States Department of Agriculture; Estados UnidosFil: Elliott, Jacalyn L.. University Of North Carolina; Estados UnidosFil: Singh, Rakhi. University Of North Carolina; Estados UnidosFil: Horn, Bruce W.. United States Department Of Agriculture; Estados UnidosFil: Dorner, Jeo W.. United States Department Of Agriculture; Estados UnidosFil: Stone, Eric A.. University Of North Carolina; Estados UnidosFil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología E Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barros, Germán Gustavo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología E Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Naik, Manjunath K.. College of Agriculture. Department of Plant Pathology; IndiaFil: Wright, Graeme C.. Department of Primary Industries; AustraliaFil: Hell, Kerstin. International Institute of Tropical Agriculture; BenínFil: Carbone, Ignazio. University Of North Carolina; Estados UnidosPublic Library Of Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10182Moore, Geromy G.; Elliott, Jacalyn L.; Singh, Rakhi; Horn, Bruce W.; Dorner, Jeo W.; et al.; Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale; Public Library Of Science; Plos Pathogens; 9; 8; 8-2013; 1-121553-7366enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003574info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757046/info:eu-repo/semantics/altIdentifier/doi/ 10.1371/journal.ppat.1003574info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:25Zoai:ri.conicet.gov.ar:11336/10182instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:25.907CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale
title Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale
spellingShingle Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale
Moore, Geromy G.
Sexuality Diversity
Aspergillus
Aflatoxin
title_short Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale
title_full Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale
title_fullStr Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale
title_full_unstemmed Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale
title_sort Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale
dc.creator.none.fl_str_mv Moore, Geromy G.
Elliott, Jacalyn L.
Singh, Rakhi
Horn, Bruce W.
Dorner, Jeo W.
Stone, Eric A.
Chulze, Sofia Noemi
Barros, Germán Gustavo
Naik, Manjunath K.
Wright, Graeme C.
Hell, Kerstin
Carbone, Ignazio
author Moore, Geromy G.
author_facet Moore, Geromy G.
Elliott, Jacalyn L.
Singh, Rakhi
Horn, Bruce W.
Dorner, Jeo W.
Stone, Eric A.
Chulze, Sofia Noemi
Barros, Germán Gustavo
Naik, Manjunath K.
Wright, Graeme C.
Hell, Kerstin
Carbone, Ignazio
author_role author
author2 Elliott, Jacalyn L.
Singh, Rakhi
Horn, Bruce W.
Dorner, Jeo W.
Stone, Eric A.
Chulze, Sofia Noemi
Barros, Germán Gustavo
Naik, Manjunath K.
Wright, Graeme C.
Hell, Kerstin
Carbone, Ignazio
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Sexuality Diversity
Aspergillus
Aflatoxin
topic Sexuality Diversity
Aspergillus
Aflatoxin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aflatoxins are produced by Aspergillus flavus and A. parasiticus in oil-rich seed and grain crops and are a serious problem in agriculture, with aflatoxin B1 being the most carcinogenic natural compound known. Sexual reproduction in these species occurs between individuals belonging to different vegetative compatibility groups (VCGs). We examined natural genetic variation in 758 isolates of A. flavus, A. parasiticus and A. minisclerotigenes sampled from single peanut fields in the United States (Georgia), Africa (Benin), Argentina (Co´rdoba), Australia (Queensland) and India (Karnataka). Analysis of DNA sequence variation across multiple intergenic regions in the aflatoxin gene clusters of A. flavus, A. parasiticus and A. minisclerotigenes revealed significant linkage disequilibrium (LD) organized into distinct blocks that are conserved across different localities, suggesting that genetic recombination is nonrandom and a global occurrence. To assess the contributions of asexual and sexual reproduction to fixation and maintenance of toxin chemotype diversity in populations from each locality/species, we tested the null hypothesis of an equal number of MAT1-1 and MAT1-2 mating-type individuals, which is indicative of a sexually recombining population. All samples were clone-corrected using multi-locus sequence typing which associates closely with VCG. For both A. flavus and A. parasiticus, when the proportions of MAT1-1 and MAT1-2 were significantly different, there was more extensive LD in the aflatoxin cluster and populations were fixed for specific toxin chemotype classes, either the non-aflatoxigenic class in A. flavus or the B1-dominant and G1-dominant classes in A. parasiticus. A mating type ratio close to 1:1 in A. flavus, A. parasiticus and A. minisclerotigenes was associated with higher recombination rates in the aflatoxin cluster and less pronounced chemotype differences in populations. This work shows that the reproductive nature of the population (more sexual versus more asexual) is predictive of aflatoxin chemotype diversity in these agriculturally important fungi.
Fil: Moore, Geromy G.. United States Department of Agriculture; Estados Unidos
Fil: Elliott, Jacalyn L.. University Of North Carolina; Estados Unidos
Fil: Singh, Rakhi. University Of North Carolina; Estados Unidos
Fil: Horn, Bruce W.. United States Department Of Agriculture; Estados Unidos
Fil: Dorner, Jeo W.. United States Department Of Agriculture; Estados Unidos
Fil: Stone, Eric A.. University Of North Carolina; Estados Unidos
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología E Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barros, Germán Gustavo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología E Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Naik, Manjunath K.. College of Agriculture. Department of Plant Pathology; India
Fil: Wright, Graeme C.. Department of Primary Industries; Australia
Fil: Hell, Kerstin. International Institute of Tropical Agriculture; Benín
Fil: Carbone, Ignazio. University Of North Carolina; Estados Unidos
description Aflatoxins are produced by Aspergillus flavus and A. parasiticus in oil-rich seed and grain crops and are a serious problem in agriculture, with aflatoxin B1 being the most carcinogenic natural compound known. Sexual reproduction in these species occurs between individuals belonging to different vegetative compatibility groups (VCGs). We examined natural genetic variation in 758 isolates of A. flavus, A. parasiticus and A. minisclerotigenes sampled from single peanut fields in the United States (Georgia), Africa (Benin), Argentina (Co´rdoba), Australia (Queensland) and India (Karnataka). Analysis of DNA sequence variation across multiple intergenic regions in the aflatoxin gene clusters of A. flavus, A. parasiticus and A. minisclerotigenes revealed significant linkage disequilibrium (LD) organized into distinct blocks that are conserved across different localities, suggesting that genetic recombination is nonrandom and a global occurrence. To assess the contributions of asexual and sexual reproduction to fixation and maintenance of toxin chemotype diversity in populations from each locality/species, we tested the null hypothesis of an equal number of MAT1-1 and MAT1-2 mating-type individuals, which is indicative of a sexually recombining population. All samples were clone-corrected using multi-locus sequence typing which associates closely with VCG. For both A. flavus and A. parasiticus, when the proportions of MAT1-1 and MAT1-2 were significantly different, there was more extensive LD in the aflatoxin cluster and populations were fixed for specific toxin chemotype classes, either the non-aflatoxigenic class in A. flavus or the B1-dominant and G1-dominant classes in A. parasiticus. A mating type ratio close to 1:1 in A. flavus, A. parasiticus and A. minisclerotigenes was associated with higher recombination rates in the aflatoxin cluster and less pronounced chemotype differences in populations. This work shows that the reproductive nature of the population (more sexual versus more asexual) is predictive of aflatoxin chemotype diversity in these agriculturally important fungi.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10182
Moore, Geromy G.; Elliott, Jacalyn L.; Singh, Rakhi; Horn, Bruce W.; Dorner, Jeo W.; et al.; Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale; Public Library Of Science; Plos Pathogens; 9; 8; 8-2013; 1-12
1553-7366
url http://hdl.handle.net/11336/10182
identifier_str_mv Moore, Geromy G.; Elliott, Jacalyn L.; Singh, Rakhi; Horn, Bruce W.; Dorner, Jeo W.; et al.; Sexuality generates diversity in the Aflatoxin Gene cluster: evidence on a global scale; Public Library Of Science; Plos Pathogens; 9; 8; 8-2013; 1-12
1553-7366
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003574
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757046/
info:eu-repo/semantics/altIdentifier/doi/ 10.1371/journal.ppat.1003574
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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