The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus

Autores
Pedrosa, Carolina da S. G.; Souza, Leticia R. Q.; Gomes, Tiago A.; de Lima, Caroline V. F.; Ledur, Pitia F.; Karmirian, Karina; Barbeito Andrés, Jimena; Costa, Marcelo do N.; Higa, Luiza M.; Rossi, Átila D.; Bellio, Maria; Tanuri, Amilcar; Prata Barbosa, Arnaldo; Tovar Moll, Fernanda; Garcez, Patricia P.; Lara, Flavio A.; Molica, Renato J. R.; Rehen, Stevens K.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The northeast (NE) region of Brazil commonly goes through drought periods, which favor cyanobacterial blooms, capable of producing neurotoxins with implications for human and animal health. The most severe dry spell in the history of Brazil occurred between 2012 and 2016. Coincidently, the highest incidence of microcephaly associated with the Zika virus (ZIKV) outbreak took place in the NE region of Brazil during the same years. In this work, we tested the hypothesis that saxitoxin (STX), a neurotoxin produced in South America by the freshwater cyanobacteria Raphidiopsis raciborskii, could have contributed to the most severe Congenital Zika Syndrome (CZS) profile described worldwide. Quality surveillance showed higher cyanobacteria amounts and STX occurrence in human drinking water sup-plies of NE compared to other regions of Brazil. Experimentally, we described that STX dou-bled the quantity of ZIKV-induced neural cell death in progenitor areas of human brain organoids, while the chronic ingestion of water contaminated with STX before and during gestation caused brain abnormalities in offspring of ZIKV-infected immunocompetent C57BL/6J mice. Our data indicate that saxitoxin-producing cyanobacteria is overspread in water reservoirs of the NE and might have acted as a co-insult to ZIKV infection in Brazil. These results raise a public health concern regarding the consequences of arbovirus outbreaks happening in areas with droughts and/or frequent freshwater cyanobacterial blooms.
Fil: Pedrosa, Carolina da S. G.. D’Or Institute for Research and Education; Brasil
Fil: Souza, Leticia R. Q.. D’Or Institute for Research and Education; Brasil
Fil: Gomes, Tiago A.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Oswaldo Cruz; Brasil
Fil: de Lima, Caroline V. F.. D’Or Institute for Research and Education; Brasil
Fil: Ledur, Pitia F.. D’Or Institute for Research and Education; Brasil
Fil: Karmirian, Karina. D’Or Institute for Research and Education; Brasil. Universidade Federal do Rio de Janeiro; Brasil
Fil: Barbeito Andrés, Jimena. Universidade Federal do Rio de Janeiro; Brasil. Universidad Nacional Arturo Jauretche. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos; Argentina
Fil: Costa, Marcelo do N.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Higa, Luiza M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Rossi, Átila D.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bellio, Maria. Universidade Federal do Rio de Janeiro; Brasil
Fil: Tanuri, Amilcar. Universidade Federal do Rio de Janeiro; Brasil
Fil: Prata Barbosa, Arnaldo. D’Or Institute for Research and Education; Brasil
Fil: Tovar Moll, Fernanda. D’Or Institute for Research and Education; Brasil. Universidade Federal do Rio de Janeiro; Brasil
Fil: Garcez, Patricia P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Lara, Flavio A.. Instituto Oswaldo Cruz; Brasil
Fil: Molica, Renato J. R.. Universidad Federal Rural Pernambuco; Brasil
Fil: Rehen, Stevens K.. D’Or Institute for Research and Education; Brasil. Universidade Federal do Rio de Janeiro; Brasil
Materia
BRAIN DEVELOPMENT
NEUROSCIENCE
BRAZIL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142401
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virusPedrosa, Carolina da S. G.Souza, Leticia R. Q.Gomes, Tiago A.de Lima, Caroline V. F.Ledur, Pitia F.Karmirian, KarinaBarbeito Andrés, JimenaCosta, Marcelo do N.Higa, Luiza M.Rossi, Átila D.Bellio, MariaTanuri, AmilcarPrata Barbosa, ArnaldoTovar Moll, FernandaGarcez, Patricia P.Lara, Flavio A.Molica, Renato J. R.Rehen, Stevens K.BRAIN DEVELOPMENTNEUROSCIENCEBRAZILhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The northeast (NE) region of Brazil commonly goes through drought periods, which favor cyanobacterial blooms, capable of producing neurotoxins with implications for human and animal health. The most severe dry spell in the history of Brazil occurred between 2012 and 2016. Coincidently, the highest incidence of microcephaly associated with the Zika virus (ZIKV) outbreak took place in the NE region of Brazil during the same years. In this work, we tested the hypothesis that saxitoxin (STX), a neurotoxin produced in South America by the freshwater cyanobacteria Raphidiopsis raciborskii, could have contributed to the most severe Congenital Zika Syndrome (CZS) profile described worldwide. Quality surveillance showed higher cyanobacteria amounts and STX occurrence in human drinking water sup-plies of NE compared to other regions of Brazil. Experimentally, we described that STX dou-bled the quantity of ZIKV-induced neural cell death in progenitor areas of human brain organoids, while the chronic ingestion of water contaminated with STX before and during gestation caused brain abnormalities in offspring of ZIKV-infected immunocompetent C57BL/6J mice. Our data indicate that saxitoxin-producing cyanobacteria is overspread in water reservoirs of the NE and might have acted as a co-insult to ZIKV infection in Brazil. These results raise a public health concern regarding the consequences of arbovirus outbreaks happening in areas with droughts and/or frequent freshwater cyanobacterial blooms.Fil: Pedrosa, Carolina da S. G.. D’Or Institute for Research and Education; BrasilFil: Souza, Leticia R. Q.. D’Or Institute for Research and Education; BrasilFil: Gomes, Tiago A.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Oswaldo Cruz; BrasilFil: de Lima, Caroline V. F.. D’Or Institute for Research and Education; BrasilFil: Ledur, Pitia F.. D’Or Institute for Research and Education; BrasilFil: Karmirian, Karina. D’Or Institute for Research and Education; Brasil. Universidade Federal do Rio de Janeiro; BrasilFil: Barbeito Andrés, Jimena. Universidade Federal do Rio de Janeiro; Brasil. Universidad Nacional Arturo Jauretche. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos; ArgentinaFil: Costa, Marcelo do N.. Universidade Federal do Rio de Janeiro; BrasilFil: Higa, Luiza M.. Universidade Federal do Rio de Janeiro; BrasilFil: Rossi, Átila D.. Universidade Federal do Rio de Janeiro; BrasilFil: Bellio, Maria. Universidade Federal do Rio de Janeiro; BrasilFil: Tanuri, Amilcar. Universidade Federal do Rio de Janeiro; BrasilFil: Prata Barbosa, Arnaldo. D’Or Institute for Research and Education; BrasilFil: Tovar Moll, Fernanda. D’Or Institute for Research and Education; Brasil. Universidade Federal do Rio de Janeiro; BrasilFil: Garcez, Patricia P.. Universidade Federal do Rio de Janeiro; BrasilFil: Lara, Flavio A.. Instituto Oswaldo Cruz; BrasilFil: Molica, Renato J. R.. Universidad Federal Rural Pernambuco; BrasilFil: Rehen, Stevens K.. D’Or Institute for Research and Education; Brasil. Universidade Federal do Rio de Janeiro; BrasilPublic Library of Science2020-03-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142401Pedrosa, Carolina da S. G.; Souza, Leticia R. Q.; Gomes, Tiago A.; de Lima, Caroline V. F.; Ledur, Pitia F.; et al.; The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus; Public Library of Science; Neglected Tropical Diseases; 14; 3; 12-3-2020; 1-131935-27271935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0008060info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0008060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:23Zoai:ri.conicet.gov.ar:11336/142401instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:24.251CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus
title The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus
spellingShingle The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus
Pedrosa, Carolina da S. G.
BRAIN DEVELOPMENT
NEUROSCIENCE
BRAZIL
title_short The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus
title_full The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus
title_fullStr The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus
title_full_unstemmed The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus
title_sort The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus
dc.creator.none.fl_str_mv Pedrosa, Carolina da S. G.
Souza, Leticia R. Q.
Gomes, Tiago A.
de Lima, Caroline V. F.
Ledur, Pitia F.
Karmirian, Karina
Barbeito Andrés, Jimena
Costa, Marcelo do N.
Higa, Luiza M.
Rossi, Átila D.
Bellio, Maria
Tanuri, Amilcar
Prata Barbosa, Arnaldo
Tovar Moll, Fernanda
Garcez, Patricia P.
Lara, Flavio A.
Molica, Renato J. R.
Rehen, Stevens K.
author Pedrosa, Carolina da S. G.
author_facet Pedrosa, Carolina da S. G.
Souza, Leticia R. Q.
Gomes, Tiago A.
de Lima, Caroline V. F.
Ledur, Pitia F.
Karmirian, Karina
Barbeito Andrés, Jimena
Costa, Marcelo do N.
Higa, Luiza M.
Rossi, Átila D.
Bellio, Maria
Tanuri, Amilcar
Prata Barbosa, Arnaldo
Tovar Moll, Fernanda
Garcez, Patricia P.
Lara, Flavio A.
Molica, Renato J. R.
Rehen, Stevens K.
author_role author
author2 Souza, Leticia R. Q.
Gomes, Tiago A.
de Lima, Caroline V. F.
Ledur, Pitia F.
Karmirian, Karina
Barbeito Andrés, Jimena
Costa, Marcelo do N.
Higa, Luiza M.
Rossi, Átila D.
Bellio, Maria
Tanuri, Amilcar
Prata Barbosa, Arnaldo
Tovar Moll, Fernanda
Garcez, Patricia P.
Lara, Flavio A.
Molica, Renato J. R.
Rehen, Stevens K.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BRAIN DEVELOPMENT
NEUROSCIENCE
BRAZIL
topic BRAIN DEVELOPMENT
NEUROSCIENCE
BRAZIL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The northeast (NE) region of Brazil commonly goes through drought periods, which favor cyanobacterial blooms, capable of producing neurotoxins with implications for human and animal health. The most severe dry spell in the history of Brazil occurred between 2012 and 2016. Coincidently, the highest incidence of microcephaly associated with the Zika virus (ZIKV) outbreak took place in the NE region of Brazil during the same years. In this work, we tested the hypothesis that saxitoxin (STX), a neurotoxin produced in South America by the freshwater cyanobacteria Raphidiopsis raciborskii, could have contributed to the most severe Congenital Zika Syndrome (CZS) profile described worldwide. Quality surveillance showed higher cyanobacteria amounts and STX occurrence in human drinking water sup-plies of NE compared to other regions of Brazil. Experimentally, we described that STX dou-bled the quantity of ZIKV-induced neural cell death in progenitor areas of human brain organoids, while the chronic ingestion of water contaminated with STX before and during gestation caused brain abnormalities in offspring of ZIKV-infected immunocompetent C57BL/6J mice. Our data indicate that saxitoxin-producing cyanobacteria is overspread in water reservoirs of the NE and might have acted as a co-insult to ZIKV infection in Brazil. These results raise a public health concern regarding the consequences of arbovirus outbreaks happening in areas with droughts and/or frequent freshwater cyanobacterial blooms.
Fil: Pedrosa, Carolina da S. G.. D’Or Institute for Research and Education; Brasil
Fil: Souza, Leticia R. Q.. D’Or Institute for Research and Education; Brasil
Fil: Gomes, Tiago A.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Oswaldo Cruz; Brasil
Fil: de Lima, Caroline V. F.. D’Or Institute for Research and Education; Brasil
Fil: Ledur, Pitia F.. D’Or Institute for Research and Education; Brasil
Fil: Karmirian, Karina. D’Or Institute for Research and Education; Brasil. Universidade Federal do Rio de Janeiro; Brasil
Fil: Barbeito Andrés, Jimena. Universidade Federal do Rio de Janeiro; Brasil. Universidad Nacional Arturo Jauretche. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos; Argentina
Fil: Costa, Marcelo do N.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Higa, Luiza M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Rossi, Átila D.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bellio, Maria. Universidade Federal do Rio de Janeiro; Brasil
Fil: Tanuri, Amilcar. Universidade Federal do Rio de Janeiro; Brasil
Fil: Prata Barbosa, Arnaldo. D’Or Institute for Research and Education; Brasil
Fil: Tovar Moll, Fernanda. D’Or Institute for Research and Education; Brasil. Universidade Federal do Rio de Janeiro; Brasil
Fil: Garcez, Patricia P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Lara, Flavio A.. Instituto Oswaldo Cruz; Brasil
Fil: Molica, Renato J. R.. Universidad Federal Rural Pernambuco; Brasil
Fil: Rehen, Stevens K.. D’Or Institute for Research and Education; Brasil. Universidade Federal do Rio de Janeiro; Brasil
description The northeast (NE) region of Brazil commonly goes through drought periods, which favor cyanobacterial blooms, capable of producing neurotoxins with implications for human and animal health. The most severe dry spell in the history of Brazil occurred between 2012 and 2016. Coincidently, the highest incidence of microcephaly associated with the Zika virus (ZIKV) outbreak took place in the NE region of Brazil during the same years. In this work, we tested the hypothesis that saxitoxin (STX), a neurotoxin produced in South America by the freshwater cyanobacteria Raphidiopsis raciborskii, could have contributed to the most severe Congenital Zika Syndrome (CZS) profile described worldwide. Quality surveillance showed higher cyanobacteria amounts and STX occurrence in human drinking water sup-plies of NE compared to other regions of Brazil. Experimentally, we described that STX dou-bled the quantity of ZIKV-induced neural cell death in progenitor areas of human brain organoids, while the chronic ingestion of water contaminated with STX before and during gestation caused brain abnormalities in offspring of ZIKV-infected immunocompetent C57BL/6J mice. Our data indicate that saxitoxin-producing cyanobacteria is overspread in water reservoirs of the NE and might have acted as a co-insult to ZIKV infection in Brazil. These results raise a public health concern regarding the consequences of arbovirus outbreaks happening in areas with droughts and/or frequent freshwater cyanobacterial blooms.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142401
Pedrosa, Carolina da S. G.; Souza, Leticia R. Q.; Gomes, Tiago A.; de Lima, Caroline V. F.; Ledur, Pitia F.; et al.; The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus; Public Library of Science; Neglected Tropical Diseases; 14; 3; 12-3-2020; 1-13
1935-2727
1935-2735
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142401
identifier_str_mv Pedrosa, Carolina da S. G.; Souza, Leticia R. Q.; Gomes, Tiago A.; de Lima, Caroline V. F.; Ledur, Pitia F.; et al.; The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus; Public Library of Science; Neglected Tropical Diseases; 14; 3; 12-3-2020; 1-13
1935-2727
1935-2735
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0008060
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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