Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome

Autores
Trabucchi, Aldana; Iacono, Ruben Francisco; Guerra, Luciano Lucas; Faccinetti, Natalia Ines; Krochik, Andrea G.; Arriazu, María C.; Poskus, Edgardo; Valdez, Silvina Noemi
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, the characterization of insulin (auto)antibodies has been described, mainly in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by Surface Plasmon Resonance (SPR) in two particular clinical cases of individuals with severe episodes of impaired glycemia. Subject 1 suffers from brittle diabetes associated with circulating insulin antibodies (IA) due to insulin treatment. Subject 2 has insulin autoantibodies (IAA) associated with hypoglycemia in spite of not being diabetic and not having ever received exogenous insulin therapy. After conventional screening for IA/IAA by radioligand binding assay (RBA), we further characterized IA/IAA in sera of both patients in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by means of SPR technology. In both cases, q values were higher and Ka values were lower than those obtained in type 1 diabetic patients, suggesting that IA/IAA:insulin immunocomplexes could be responsible for the uncontrolled glycemia. Moreover, subject 1 had a predominat IgG1 response and subject 2 had an IgG3 response. In conclusion, SPR technology is useful for the complete characterization of IA/IAA which can be used in special cases where the simple positive/negative determination is not enough to achieve a detailed description of the disease fisiopathology.
Fil: Trabucchi, Aldana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Iacono, Ruben Francisco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Guerra, Luciano Lucas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Faccinetti, Natalia Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Krochik, Andrea G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Arriazu, María C.. Hospital Comunitario Privado; Argentina
Fil: Poskus, Edgardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Valdez, Silvina Noemi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
INSULIN (AUTO)ANTIBODIES
HYPO AND HYPERGLYCEMIA
SURFACE PLASMON RESONANCE
CONCENTRATION AND AFFINITY OF (AUTO)ANTIBODIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8055
Acceder
id CONICETDig_7db401d6c8e8809973d59f31a43cfdf5
oai_identifier_str oai:ri.conicet.gov.ar:11336/8055
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune SyndromeTrabucchi, AldanaIacono, Ruben FranciscoGuerra, Luciano LucasFaccinetti, Natalia InesKrochik, Andrea G.Arriazu, María C.Poskus, EdgardoValdez, Silvina NoemiINSULIN (AUTO)ANTIBODIESHYPO AND HYPERGLYCEMIASURFACE PLASMON RESONANCECONCENTRATION AND AFFINITY OF (AUTO)ANTIBODIEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In this study, the characterization of insulin (auto)antibodies has been described, mainly in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by Surface Plasmon Resonance (SPR) in two particular clinical cases of individuals with severe episodes of impaired glycemia. Subject 1 suffers from brittle diabetes associated with circulating insulin antibodies (IA) due to insulin treatment. Subject 2 has insulin autoantibodies (IAA) associated with hypoglycemia in spite of not being diabetic and not having ever received exogenous insulin therapy. After conventional screening for IA/IAA by radioligand binding assay (RBA), we further characterized IA/IAA in sera of both patients in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by means of SPR technology. In both cases, q values were higher and Ka values were lower than those obtained in type 1 diabetic patients, suggesting that IA/IAA:insulin immunocomplexes could be responsible for the uncontrolled glycemia. Moreover, subject 1 had a predominat IgG1 response and subject 2 had an IgG3 response. In conclusion, SPR technology is useful for the complete characterization of IA/IAA which can be used in special cases where the simple positive/negative determination is not enough to achieve a detailed description of the disease fisiopathology.Fil: Trabucchi, Aldana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Iacono, Ruben Francisco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Guerra, Luciano Lucas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Faccinetti, Natalia Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Krochik, Andrea G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Arriazu, María C.. Hospital Comunitario Privado; ArgentinaFil: Poskus, Edgardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Valdez, Silvina Noemi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaPublic Library Of Science2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8055Trabucchi, Aldana; Iacono, Ruben Francisco; Guerra, Luciano Lucas; Faccinetti, Natalia Ines; Krochik, Andrea G.; et al.; Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome; Public Library Of Science; Plos One; 8; 12; 12-2013; e84099-e840991932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0084099info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0084099info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875527/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:58Zoai:ri.conicet.gov.ar:11336/8055instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:59.258CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome
title Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome
spellingShingle Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome
Trabucchi, Aldana
INSULIN (AUTO)ANTIBODIES
HYPO AND HYPERGLYCEMIA
SURFACE PLASMON RESONANCE
CONCENTRATION AND AFFINITY OF (AUTO)ANTIBODIES
title_short Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome
title_full Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome
title_fullStr Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome
title_full_unstemmed Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome
title_sort Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome
dc.creator.none.fl_str_mv Trabucchi, Aldana
Iacono, Ruben Francisco
Guerra, Luciano Lucas
Faccinetti, Natalia Ines
Krochik, Andrea G.
Arriazu, María C.
Poskus, Edgardo
Valdez, Silvina Noemi
author Trabucchi, Aldana
author_facet Trabucchi, Aldana
Iacono, Ruben Francisco
Guerra, Luciano Lucas
Faccinetti, Natalia Ines
Krochik, Andrea G.
Arriazu, María C.
Poskus, Edgardo
Valdez, Silvina Noemi
author_role author
author2 Iacono, Ruben Francisco
Guerra, Luciano Lucas
Faccinetti, Natalia Ines
Krochik, Andrea G.
Arriazu, María C.
Poskus, Edgardo
Valdez, Silvina Noemi
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INSULIN (AUTO)ANTIBODIES
HYPO AND HYPERGLYCEMIA
SURFACE PLASMON RESONANCE
CONCENTRATION AND AFFINITY OF (AUTO)ANTIBODIES
topic INSULIN (AUTO)ANTIBODIES
HYPO AND HYPERGLYCEMIA
SURFACE PLASMON RESONANCE
CONCENTRATION AND AFFINITY OF (AUTO)ANTIBODIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this study, the characterization of insulin (auto)antibodies has been described, mainly in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by Surface Plasmon Resonance (SPR) in two particular clinical cases of individuals with severe episodes of impaired glycemia. Subject 1 suffers from brittle diabetes associated with circulating insulin antibodies (IA) due to insulin treatment. Subject 2 has insulin autoantibodies (IAA) associated with hypoglycemia in spite of not being diabetic and not having ever received exogenous insulin therapy. After conventional screening for IA/IAA by radioligand binding assay (RBA), we further characterized IA/IAA in sera of both patients in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by means of SPR technology. In both cases, q values were higher and Ka values were lower than those obtained in type 1 diabetic patients, suggesting that IA/IAA:insulin immunocomplexes could be responsible for the uncontrolled glycemia. Moreover, subject 1 had a predominat IgG1 response and subject 2 had an IgG3 response. In conclusion, SPR technology is useful for the complete characterization of IA/IAA which can be used in special cases where the simple positive/negative determination is not enough to achieve a detailed description of the disease fisiopathology.
Fil: Trabucchi, Aldana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Iacono, Ruben Francisco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Guerra, Luciano Lucas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Faccinetti, Natalia Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Krochik, Andrea G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
Fil: Arriazu, María C.. Hospital Comunitario Privado; Argentina
Fil: Poskus, Edgardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Valdez, Silvina Noemi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description In this study, the characterization of insulin (auto)antibodies has been described, mainly in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by Surface Plasmon Resonance (SPR) in two particular clinical cases of individuals with severe episodes of impaired glycemia. Subject 1 suffers from brittle diabetes associated with circulating insulin antibodies (IA) due to insulin treatment. Subject 2 has insulin autoantibodies (IAA) associated with hypoglycemia in spite of not being diabetic and not having ever received exogenous insulin therapy. After conventional screening for IA/IAA by radioligand binding assay (RBA), we further characterized IA/IAA in sera of both patients in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by means of SPR technology. In both cases, q values were higher and Ka values were lower than those obtained in type 1 diabetic patients, suggesting that IA/IAA:insulin immunocomplexes could be responsible for the uncontrolled glycemia. Moreover, subject 1 had a predominat IgG1 response and subject 2 had an IgG3 response. In conclusion, SPR technology is useful for the complete characterization of IA/IAA which can be used in special cases where the simple positive/negative determination is not enough to achieve a detailed description of the disease fisiopathology.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8055
Trabucchi, Aldana; Iacono, Ruben Francisco; Guerra, Luciano Lucas; Faccinetti, Natalia Ines; Krochik, Andrea G.; et al.; Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome; Public Library Of Science; Plos One; 8; 12; 12-2013; e84099-e84099
1932-6203
url http://hdl.handle.net/11336/8055
identifier_str_mv Trabucchi, Aldana; Iacono, Ruben Francisco; Guerra, Luciano Lucas; Faccinetti, Natalia Ines; Krochik, Andrea G.; et al.; Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome; Public Library Of Science; Plos One; 8; 12; 12-2013; e84099-e84099
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0084099
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0084099
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875527/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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