Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification

Autores
Urtasun, Nicolás; Baieli, Fernanda; Romasanta, Pablo Nicolas; Fernández, Marisa Mariel; Malchiodi, Emilio Luis; Cascone, Osvaldo; Wolman, Federico Javier; Miranda, Maria Victoria
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine lactoferricin (Lfcin B) belongs to the antimicrobial peptide family, which is the first line of defense against pathogens in many organisms. Lfcin B has important applications due to its antiviral, antifungal, antiparasitic, anticancer/tumor and antibacterial activity. In this work, we tested five triazine dyes for Lfcin B affinity interactions using Surface Plasmon Resonance (SPR) technology. Red HE-3B and Yellow HE-4R dyes were selected and immobilized on a Sepharose-4B matrix for further purification studies. Recombinant Lfcin B was expressed as a fusion protein with Glutathione -S- Transferase (Lfcin B-GST) by using baculovirus expression vector system and the dye-Sepharose matrices were assayed for Lfcin B-GST adsorption and subsequent elution. The Yellow HE-4R-Sepharose matrix was specific for Lfcin B and allowed adsorption of Lfcin B-GST directly from the culture medium even at high salt concentration. This novel application of SPR to screen possible dye-peptide interactions could be relevant to purify other peptides or proteins by using low-cost dye-affinity chromatography.
Fil: Urtasun, Nicolás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
Fil: Baieli, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
Fil: Romasanta, Pablo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;
Fil: Fernández, Marisa Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;
Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
Fil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
Fil: Miranda, Maria Victoria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
Materia
Baculovirus
Bovine Lactoferricin
Dye Affinity Chromatography
Surface Plasmon Resonance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1911
Acceder
id CONICETDig_f014f814ffe1c5ceb2818216fafd986e
oai_identifier_str oai:ri.conicet.gov.ar:11336/1911
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purificationUrtasun, NicolásBaieli, FernandaRomasanta, Pablo NicolasFernández, Marisa MarielMalchiodi, Emilio LuisCascone, OsvaldoWolman, Federico JavierMiranda, Maria VictoriaBaculovirusBovine LactoferricinDye Affinity ChromatographySurface Plasmon Resonancehttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Bovine lactoferricin (Lfcin B) belongs to the antimicrobial peptide family, which is the first line of defense against pathogens in many organisms. Lfcin B has important applications due to its antiviral, antifungal, antiparasitic, anticancer/tumor and antibacterial activity. In this work, we tested five triazine dyes for Lfcin B affinity interactions using Surface Plasmon Resonance (SPR) technology. Red HE-3B and Yellow HE-4R dyes were selected and immobilized on a Sepharose-4B matrix for further purification studies. Recombinant Lfcin B was expressed as a fusion protein with Glutathione -S- Transferase (Lfcin B-GST) by using baculovirus expression vector system and the dye-Sepharose matrices were assayed for Lfcin B-GST adsorption and subsequent elution. The Yellow HE-4R-Sepharose matrix was specific for Lfcin B and allowed adsorption of Lfcin B-GST directly from the culture medium even at high salt concentration. This novel application of SPR to screen possible dye-peptide interactions could be relevant to purify other peptides or proteins by using low-cost dye-affinity chromatography.Fil: Urtasun, Nicolás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;Fil: Baieli, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;Fil: Romasanta, Pablo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;Fil: Fernández, Marisa Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;Fil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;Fil: Miranda, Maria Victoria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;Elsevier2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1911Urtasun, Nicolás; Baieli, Fernanda; Romasanta, Pablo Nicolas; Fernández, Marisa Mariel; Malchiodi, Emilio Luis; et al.; Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification; Elsevier; Process Biochemistry; 48; 12; 12-2013; 1972-19790032-9592enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2013.07.018info:eu-repo/semantics/altIdentifier/url/http://ac.els-cdn.com/S1359511313004017/1-s2.0-S1359511313004017-main.pdf?_tid=15869a6e-638a-11e4-9e6b-00000aab0f02&acdnat=1415040724_1832cad6ca44be763d069773da7667f0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:07Zoai:ri.conicet.gov.ar:11336/1911instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:08.228CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification
title Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification
spellingShingle Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification
Urtasun, Nicolás
Baculovirus
Bovine Lactoferricin
Dye Affinity Chromatography
Surface Plasmon Resonance
title_short Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification
title_full Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification
title_fullStr Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification
title_full_unstemmed Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification
title_sort Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification
dc.creator.none.fl_str_mv Urtasun, Nicolás
Baieli, Fernanda
Romasanta, Pablo Nicolas
Fernández, Marisa Mariel
Malchiodi, Emilio Luis
Cascone, Osvaldo
Wolman, Federico Javier
Miranda, Maria Victoria
author Urtasun, Nicolás
author_facet Urtasun, Nicolás
Baieli, Fernanda
Romasanta, Pablo Nicolas
Fernández, Marisa Mariel
Malchiodi, Emilio Luis
Cascone, Osvaldo
Wolman, Federico Javier
Miranda, Maria Victoria
author_role author
author2 Baieli, Fernanda
Romasanta, Pablo Nicolas
Fernández, Marisa Mariel
Malchiodi, Emilio Luis
Cascone, Osvaldo
Wolman, Federico Javier
Miranda, Maria Victoria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Baculovirus
Bovine Lactoferricin
Dye Affinity Chromatography
Surface Plasmon Resonance
topic Baculovirus
Bovine Lactoferricin
Dye Affinity Chromatography
Surface Plasmon Resonance
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Bovine lactoferricin (Lfcin B) belongs to the antimicrobial peptide family, which is the first line of defense against pathogens in many organisms. Lfcin B has important applications due to its antiviral, antifungal, antiparasitic, anticancer/tumor and antibacterial activity. In this work, we tested five triazine dyes for Lfcin B affinity interactions using Surface Plasmon Resonance (SPR) technology. Red HE-3B and Yellow HE-4R dyes were selected and immobilized on a Sepharose-4B matrix for further purification studies. Recombinant Lfcin B was expressed as a fusion protein with Glutathione -S- Transferase (Lfcin B-GST) by using baculovirus expression vector system and the dye-Sepharose matrices were assayed for Lfcin B-GST adsorption and subsequent elution. The Yellow HE-4R-Sepharose matrix was specific for Lfcin B and allowed adsorption of Lfcin B-GST directly from the culture medium even at high salt concentration. This novel application of SPR to screen possible dye-peptide interactions could be relevant to purify other peptides or proteins by using low-cost dye-affinity chromatography.
Fil: Urtasun, Nicolás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
Fil: Baieli, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
Fil: Romasanta, Pablo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;
Fil: Fernández, Marisa Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;
Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
Fil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
Fil: Miranda, Maria Victoria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina;
description Bovine lactoferricin (Lfcin B) belongs to the antimicrobial peptide family, which is the first line of defense against pathogens in many organisms. Lfcin B has important applications due to its antiviral, antifungal, antiparasitic, anticancer/tumor and antibacterial activity. In this work, we tested five triazine dyes for Lfcin B affinity interactions using Surface Plasmon Resonance (SPR) technology. Red HE-3B and Yellow HE-4R dyes were selected and immobilized on a Sepharose-4B matrix for further purification studies. Recombinant Lfcin B was expressed as a fusion protein with Glutathione -S- Transferase (Lfcin B-GST) by using baculovirus expression vector system and the dye-Sepharose matrices were assayed for Lfcin B-GST adsorption and subsequent elution. The Yellow HE-4R-Sepharose matrix was specific for Lfcin B and allowed adsorption of Lfcin B-GST directly from the culture medium even at high salt concentration. This novel application of SPR to screen possible dye-peptide interactions could be relevant to purify other peptides or proteins by using low-cost dye-affinity chromatography.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1911
Urtasun, Nicolás; Baieli, Fernanda; Romasanta, Pablo Nicolas; Fernández, Marisa Mariel; Malchiodi, Emilio Luis; et al.; Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification; Elsevier; Process Biochemistry; 48; 12; 12-2013; 1972-1979
0032-9592
url http://hdl.handle.net/11336/1911
identifier_str_mv Urtasun, Nicolás; Baieli, Fernanda; Romasanta, Pablo Nicolas; Fernández, Marisa Mariel; Malchiodi, Emilio Luis; et al.; Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification; Elsevier; Process Biochemistry; 48; 12; 12-2013; 1972-1979
0032-9592
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2013.07.018
info:eu-repo/semantics/altIdentifier/url/http://ac.els-cdn.com/S1359511313004017/1-s2.0-S1359511313004017-main.pdf?_tid=15869a6e-638a-11e4-9e6b-00000aab0f02&acdnat=1415040724_1832cad6ca44be763d069773da7667f0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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