Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells

Autores
Liu, Kang; Idoyaga, Juliana; Charalambous, Anna; Fujii, Shin Ichiro; Bonito, Anthony; Mordoh, Jose; Wainstok, Rosa; Bai, Xue Feng; Liu, Yang; Steinman, Ralph M.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
If irradiated tumor cells could be rendered immunogenic, they would provide a safe, broad, and patient-specific array of antigens for immunotherapies. Prior approaches have emphasized genetic transduction of live tumor cells to express cytokines, costimulators, and surrogate foreign antigens. We asked if immunity could be achieved by delivering irradiated, major histocompatibility complex-negative plasmacytoma cells to maturing mouse dendritic cells (DCs) within lymphoid organs. Tumor cells injected intravenously (i.v.) were captured by splenic DCs, whereas subcutaneous (s.c.) injection led only to weak uptake in lymph node or spleen. The natural killer T (NKT) cells mobilizing glycolipid α-galactosyl ceramide, used to mature splenic DCs, served as an effective adjuvant to induce protective immunity. This adjuvant function was mimicked by a combination of poly IC and agonistic αCD40 antibody. The adjuvant glycolipid had to be coadministered with tumor cells i.v. rather than s.c. Specific resistance was generated both to a plasmacytoma and lymphoma. The resistance afforded by a single vaccination lasted >2 mo and required both CD4+ and CD8+ T cells. Mature tumor capturing DCs stimulated the differentiation of P1A tumor antigen-specific, CD8+ T cells and uniquely transferred tumor resistance to naive mice. Therefore, the access of dying tumor cells to DCs that are maturing to activated NKT cells efficiently induces long-lived adaptive resistance.
Fil: Liu, Kang. The Rockefeller University; Estados Unidos
Fil: Idoyaga, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Charalambous, Anna. The Rockefeller University; Estados Unidos
Fil: Fujii, Shin Ichiro. The Rockefeller University; Estados Unidos
Fil: Bonito, Anthony. The Rockefeller University; Estados Unidos
Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Wainstok, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bai, Xue Feng. Ohio State University; Estados Unidos
Fil: Liu, Yang. Ohio State University; Estados Unidos
Fil: Steinman, Ralph M.. The Rockefeller University; Estados Unidos
Materia
Dendritic Cells
Immunity
Tumors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39072
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/39072
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cellsLiu, KangIdoyaga, JulianaCharalambous, AnnaFujii, Shin IchiroBonito, AnthonyMordoh, JoseWainstok, RosaBai, Xue FengLiu, YangSteinman, Ralph M.Dendritic CellsImmunityTumorshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3If irradiated tumor cells could be rendered immunogenic, they would provide a safe, broad, and patient-specific array of antigens for immunotherapies. Prior approaches have emphasized genetic transduction of live tumor cells to express cytokines, costimulators, and surrogate foreign antigens. We asked if immunity could be achieved by delivering irradiated, major histocompatibility complex-negative plasmacytoma cells to maturing mouse dendritic cells (DCs) within lymphoid organs. Tumor cells injected intravenously (i.v.) were captured by splenic DCs, whereas subcutaneous (s.c.) injection led only to weak uptake in lymph node or spleen. The natural killer T (NKT) cells mobilizing glycolipid α-galactosyl ceramide, used to mature splenic DCs, served as an effective adjuvant to induce protective immunity. This adjuvant function was mimicked by a combination of poly IC and agonistic αCD40 antibody. The adjuvant glycolipid had to be coadministered with tumor cells i.v. rather than s.c. Specific resistance was generated both to a plasmacytoma and lymphoma. The resistance afforded by a single vaccination lasted >2 mo and required both CD4+ and CD8+ T cells. Mature tumor capturing DCs stimulated the differentiation of P1A tumor antigen-specific, CD8+ T cells and uniquely transferred tumor resistance to naive mice. Therefore, the access of dying tumor cells to DCs that are maturing to activated NKT cells efficiently induces long-lived adaptive resistance.Fil: Liu, Kang. The Rockefeller University; Estados UnidosFil: Idoyaga, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Charalambous, Anna. The Rockefeller University; Estados UnidosFil: Fujii, Shin Ichiro. The Rockefeller University; Estados UnidosFil: Bonito, Anthony. The Rockefeller University; Estados UnidosFil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Wainstok, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bai, Xue Feng. Ohio State University; Estados UnidosFil: Liu, Yang. Ohio State University; Estados UnidosFil: Steinman, Ralph M.. The Rockefeller University; Estados UnidosRockefeller University Press2005-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39072Liu, Kang; Idoyaga, Juliana; Charalambous, Anna; Fujii, Shin Ichiro; Bonito, Anthony; et al.; Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells; Rockefeller University Press; Journal Of Experimental Medicine; 202; 11; 12-2005; 1507-15160022-10071540-9538CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jem.rupress.org/content/202/11/1507.longinfo:eu-repo/semantics/altIdentifier/doi/10.1084/jem.20050956info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:54Zoai:ri.conicet.gov.ar:11336/39072instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:55.093CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells
title Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells
spellingShingle Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells
Liu, Kang
Dendritic Cells
Immunity
Tumors
title_short Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells
title_full Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells
title_fullStr Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells
title_full_unstemmed Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells
title_sort Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells
dc.creator.none.fl_str_mv Liu, Kang
Idoyaga, Juliana
Charalambous, Anna
Fujii, Shin Ichiro
Bonito, Anthony
Mordoh, Jose
Wainstok, Rosa
Bai, Xue Feng
Liu, Yang
Steinman, Ralph M.
author Liu, Kang
author_facet Liu, Kang
Idoyaga, Juliana
Charalambous, Anna
Fujii, Shin Ichiro
Bonito, Anthony
Mordoh, Jose
Wainstok, Rosa
Bai, Xue Feng
Liu, Yang
Steinman, Ralph M.
author_role author
author2 Idoyaga, Juliana
Charalambous, Anna
Fujii, Shin Ichiro
Bonito, Anthony
Mordoh, Jose
Wainstok, Rosa
Bai, Xue Feng
Liu, Yang
Steinman, Ralph M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dendritic Cells
Immunity
Tumors
topic Dendritic Cells
Immunity
Tumors
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv If irradiated tumor cells could be rendered immunogenic, they would provide a safe, broad, and patient-specific array of antigens for immunotherapies. Prior approaches have emphasized genetic transduction of live tumor cells to express cytokines, costimulators, and surrogate foreign antigens. We asked if immunity could be achieved by delivering irradiated, major histocompatibility complex-negative plasmacytoma cells to maturing mouse dendritic cells (DCs) within lymphoid organs. Tumor cells injected intravenously (i.v.) were captured by splenic DCs, whereas subcutaneous (s.c.) injection led only to weak uptake in lymph node or spleen. The natural killer T (NKT) cells mobilizing glycolipid α-galactosyl ceramide, used to mature splenic DCs, served as an effective adjuvant to induce protective immunity. This adjuvant function was mimicked by a combination of poly IC and agonistic αCD40 antibody. The adjuvant glycolipid had to be coadministered with tumor cells i.v. rather than s.c. Specific resistance was generated both to a plasmacytoma and lymphoma. The resistance afforded by a single vaccination lasted >2 mo and required both CD4+ and CD8+ T cells. Mature tumor capturing DCs stimulated the differentiation of P1A tumor antigen-specific, CD8+ T cells and uniquely transferred tumor resistance to naive mice. Therefore, the access of dying tumor cells to DCs that are maturing to activated NKT cells efficiently induces long-lived adaptive resistance.
Fil: Liu, Kang. The Rockefeller University; Estados Unidos
Fil: Idoyaga, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Charalambous, Anna. The Rockefeller University; Estados Unidos
Fil: Fujii, Shin Ichiro. The Rockefeller University; Estados Unidos
Fil: Bonito, Anthony. The Rockefeller University; Estados Unidos
Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Wainstok, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bai, Xue Feng. Ohio State University; Estados Unidos
Fil: Liu, Yang. Ohio State University; Estados Unidos
Fil: Steinman, Ralph M.. The Rockefeller University; Estados Unidos
description If irradiated tumor cells could be rendered immunogenic, they would provide a safe, broad, and patient-specific array of antigens for immunotherapies. Prior approaches have emphasized genetic transduction of live tumor cells to express cytokines, costimulators, and surrogate foreign antigens. We asked if immunity could be achieved by delivering irradiated, major histocompatibility complex-negative plasmacytoma cells to maturing mouse dendritic cells (DCs) within lymphoid organs. Tumor cells injected intravenously (i.v.) were captured by splenic DCs, whereas subcutaneous (s.c.) injection led only to weak uptake in lymph node or spleen. The natural killer T (NKT) cells mobilizing glycolipid α-galactosyl ceramide, used to mature splenic DCs, served as an effective adjuvant to induce protective immunity. This adjuvant function was mimicked by a combination of poly IC and agonistic αCD40 antibody. The adjuvant glycolipid had to be coadministered with tumor cells i.v. rather than s.c. Specific resistance was generated both to a plasmacytoma and lymphoma. The resistance afforded by a single vaccination lasted >2 mo and required both CD4+ and CD8+ T cells. Mature tumor capturing DCs stimulated the differentiation of P1A tumor antigen-specific, CD8+ T cells and uniquely transferred tumor resistance to naive mice. Therefore, the access of dying tumor cells to DCs that are maturing to activated NKT cells efficiently induces long-lived adaptive resistance.
publishDate 2005
dc.date.none.fl_str_mv 2005-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39072
Liu, Kang; Idoyaga, Juliana; Charalambous, Anna; Fujii, Shin Ichiro; Bonito, Anthony; et al.; Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells; Rockefeller University Press; Journal Of Experimental Medicine; 202; 11; 12-2005; 1507-1516
0022-1007
1540-9538
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39072
identifier_str_mv Liu, Kang; Idoyaga, Juliana; Charalambous, Anna; Fujii, Shin Ichiro; Bonito, Anthony; et al.; Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells; Rockefeller University Press; Journal Of Experimental Medicine; 202; 11; 12-2005; 1507-1516
0022-1007
1540-9538
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jem.rupress.org/content/202/11/1507.long
info:eu-repo/semantics/altIdentifier/doi/10.1084/jem.20050956
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Rockefeller University Press
publisher.none.fl_str_mv Rockefeller University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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