Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells
- Autores
- Liu, K.; Idoyaga, J.; Charalambous, A.; Fujii, S.-I.; Bonito, A.; Mordoh, J.; Wainstok, R.; Bai, X.-F.; Liu, Y.; Steinman, R.M.
- Año de publicación
- 2005
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- If irradiated tumor cells could be rendered immunogenic, they would provide a safe, broad, and patient-specific array of antigens for immunotherapies. Prior approaches have emphasized genetic transduction of live tumor cells to express cytokines, costimulators, and surrogate foreign antigens. We asked if immunity could be achieved by delivering irradiated, major histocompatibility complex-negative plasmacytoma cells to maturing mouse dendritic cells (DCs) within lymphoid organs. Tumor cells injected intravenously (i.v.) were captured by splenic DCs, whereas subcutaneous (s.c.) injection led only to weak uptake in lymph node or spleen. The natural killer T (NKT) cells mobilizing glycolipid α-galactosyl ceramide, used to mature splenic DCs, served as an effective adjuvant to induce protective immunity. This adjuvant function was mimicked by a combination of poly IC and agonistic αCD40 antibody. The adjuvant glycolipid had to be coadministered with tumor cells i.v. rather than s.c. Specific resistance was generated both to a plasmacytoma and lymphoma. The resistance afforded by a single vaccination lasted >2 mo and required both CD4+ and CD8+ T cells. Mature tumor capturing DCs stimulated the differentiation of P1A tumor antigen-specific, CD8+ T cells and uniquely transferred tumor resistance to naive mice. Therefore, the access of dying tumor cells to DCs that are maturing to activated NKT cells efficiently induces long-lived adaptive resistance. JEM © The Rockefeller University Press.
Fil:Idoyaga, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- J. Exp. Med. 2005;202(11):1507-1516
- Materia
-
adjuvant
alpha CD40 antibody
alpha galactosylceramide
antibody
CD4 antigen
CD8 antigen
glycolipid
major histocompatibility antigen class 1
polyinosinic polycytidylic acid
tumor antigen
unclassified drug
animal cell
animal experiment
animal model
article
cell differentiation
cell maturation
controlled study
dendritic cell
drug delivery system
drug uptake
female
irradiation
lymph node
lymphoid organ
lymphoma
major histocompatibility complex
mouse
natural killer cell
nonhuman
plasmacytoma
priority journal
spleen
spleen cell
T lymphocyte
tumor immunity
tumor resistance
vaccination
Adjuvants, Immunologic
Animals
Antigen Presentation
Antigens, Neoplasm
Cancer Vaccines
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Cell Differentiation
Dendritic Cells
Female
Gamma Rays
Immunotherapy, Active
Killer Cells, Natural
Lymph Nodes
Lymphocyte Activation
Lymphoma
Mice
Mice, Inbred BALB C
Neoplasm Transplantation
Neoplasms, Experimental
Plasmacytoma
Spleen - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_00221007_v202_n11_p1507_Liu
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Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cellsLiu, K.Idoyaga, J.Charalambous, A.Fujii, S.-I.Bonito, A.Mordoh, J.Wainstok, R.Bai, X.-F.Liu, Y.Steinman, R.M.adjuvantalpha CD40 antibodyalpha galactosylceramideantibodyCD4 antigenCD8 antigenglycolipidmajor histocompatibility antigen class 1polyinosinic polycytidylic acidtumor antigenunclassified druganimal cellanimal experimentanimal modelarticlecell differentiationcell maturationcontrolled studydendritic celldrug delivery systemdrug uptakefemaleirradiationlymph nodelymphoid organlymphomamajor histocompatibility complexmousenatural killer cellnonhumanplasmacytomapriority journalspleenspleen cellT lymphocytetumor immunitytumor resistancevaccinationAdjuvants, ImmunologicAnimalsAntigen PresentationAntigens, NeoplasmCancer VaccinesCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell DifferentiationDendritic CellsFemaleGamma RaysImmunotherapy, ActiveKiller Cells, NaturalLymph NodesLymphocyte ActivationLymphomaMiceMice, Inbred BALB CNeoplasm TransplantationNeoplasms, ExperimentalPlasmacytomaSpleenIf irradiated tumor cells could be rendered immunogenic, they would provide a safe, broad, and patient-specific array of antigens for immunotherapies. Prior approaches have emphasized genetic transduction of live tumor cells to express cytokines, costimulators, and surrogate foreign antigens. We asked if immunity could be achieved by delivering irradiated, major histocompatibility complex-negative plasmacytoma cells to maturing mouse dendritic cells (DCs) within lymphoid organs. Tumor cells injected intravenously (i.v.) were captured by splenic DCs, whereas subcutaneous (s.c.) injection led only to weak uptake in lymph node or spleen. The natural killer T (NKT) cells mobilizing glycolipid α-galactosyl ceramide, used to mature splenic DCs, served as an effective adjuvant to induce protective immunity. This adjuvant function was mimicked by a combination of poly IC and agonistic αCD40 antibody. The adjuvant glycolipid had to be coadministered with tumor cells i.v. rather than s.c. Specific resistance was generated both to a plasmacytoma and lymphoma. The resistance afforded by a single vaccination lasted >2 mo and required both CD4+ and CD8+ T cells. Mature tumor capturing DCs stimulated the differentiation of P1A tumor antigen-specific, CD8+ T cells and uniquely transferred tumor resistance to naive mice. Therefore, the access of dying tumor cells to DCs that are maturing to activated NKT cells efficiently induces long-lived adaptive resistance. JEM © The Rockefeller University Press.Fil:Idoyaga, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00221007_v202_n11_p1507_LiuJ. Exp. Med. 2005;202(11):1507-1516reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-16T09:30:20Zpaperaa:paper_00221007_v202_n11_p1507_LiuInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:30:22.464Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells |
title |
Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells |
spellingShingle |
Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells Liu, K. adjuvant alpha CD40 antibody alpha galactosylceramide antibody CD4 antigen CD8 antigen glycolipid major histocompatibility antigen class 1 polyinosinic polycytidylic acid tumor antigen unclassified drug animal cell animal experiment animal model article cell differentiation cell maturation controlled study dendritic cell drug delivery system drug uptake female irradiation lymph node lymphoid organ lymphoma major histocompatibility complex mouse natural killer cell nonhuman plasmacytoma priority journal spleen spleen cell T lymphocyte tumor immunity tumor resistance vaccination Adjuvants, Immunologic Animals Antigen Presentation Antigens, Neoplasm Cancer Vaccines CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Cell Differentiation Dendritic Cells Female Gamma Rays Immunotherapy, Active Killer Cells, Natural Lymph Nodes Lymphocyte Activation Lymphoma Mice Mice, Inbred BALB C Neoplasm Transplantation Neoplasms, Experimental Plasmacytoma Spleen |
title_short |
Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells |
title_full |
Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells |
title_fullStr |
Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells |
title_full_unstemmed |
Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells |
title_sort |
Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells |
dc.creator.none.fl_str_mv |
Liu, K. Idoyaga, J. Charalambous, A. Fujii, S.-I. Bonito, A. Mordoh, J. Wainstok, R. Bai, X.-F. Liu, Y. Steinman, R.M. |
author |
Liu, K. |
author_facet |
Liu, K. Idoyaga, J. Charalambous, A. Fujii, S.-I. Bonito, A. Mordoh, J. Wainstok, R. Bai, X.-F. Liu, Y. Steinman, R.M. |
author_role |
author |
author2 |
Idoyaga, J. Charalambous, A. Fujii, S.-I. Bonito, A. Mordoh, J. Wainstok, R. Bai, X.-F. Liu, Y. Steinman, R.M. |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
adjuvant alpha CD40 antibody alpha galactosylceramide antibody CD4 antigen CD8 antigen glycolipid major histocompatibility antigen class 1 polyinosinic polycytidylic acid tumor antigen unclassified drug animal cell animal experiment animal model article cell differentiation cell maturation controlled study dendritic cell drug delivery system drug uptake female irradiation lymph node lymphoid organ lymphoma major histocompatibility complex mouse natural killer cell nonhuman plasmacytoma priority journal spleen spleen cell T lymphocyte tumor immunity tumor resistance vaccination Adjuvants, Immunologic Animals Antigen Presentation Antigens, Neoplasm Cancer Vaccines CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Cell Differentiation Dendritic Cells Female Gamma Rays Immunotherapy, Active Killer Cells, Natural Lymph Nodes Lymphocyte Activation Lymphoma Mice Mice, Inbred BALB C Neoplasm Transplantation Neoplasms, Experimental Plasmacytoma Spleen |
topic |
adjuvant alpha CD40 antibody alpha galactosylceramide antibody CD4 antigen CD8 antigen glycolipid major histocompatibility antigen class 1 polyinosinic polycytidylic acid tumor antigen unclassified drug animal cell animal experiment animal model article cell differentiation cell maturation controlled study dendritic cell drug delivery system drug uptake female irradiation lymph node lymphoid organ lymphoma major histocompatibility complex mouse natural killer cell nonhuman plasmacytoma priority journal spleen spleen cell T lymphocyte tumor immunity tumor resistance vaccination Adjuvants, Immunologic Animals Antigen Presentation Antigens, Neoplasm Cancer Vaccines CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Cell Differentiation Dendritic Cells Female Gamma Rays Immunotherapy, Active Killer Cells, Natural Lymph Nodes Lymphocyte Activation Lymphoma Mice Mice, Inbred BALB C Neoplasm Transplantation Neoplasms, Experimental Plasmacytoma Spleen |
dc.description.none.fl_txt_mv |
If irradiated tumor cells could be rendered immunogenic, they would provide a safe, broad, and patient-specific array of antigens for immunotherapies. Prior approaches have emphasized genetic transduction of live tumor cells to express cytokines, costimulators, and surrogate foreign antigens. We asked if immunity could be achieved by delivering irradiated, major histocompatibility complex-negative plasmacytoma cells to maturing mouse dendritic cells (DCs) within lymphoid organs. Tumor cells injected intravenously (i.v.) were captured by splenic DCs, whereas subcutaneous (s.c.) injection led only to weak uptake in lymph node or spleen. The natural killer T (NKT) cells mobilizing glycolipid α-galactosyl ceramide, used to mature splenic DCs, served as an effective adjuvant to induce protective immunity. This adjuvant function was mimicked by a combination of poly IC and agonistic αCD40 antibody. The adjuvant glycolipid had to be coadministered with tumor cells i.v. rather than s.c. Specific resistance was generated both to a plasmacytoma and lymphoma. The resistance afforded by a single vaccination lasted >2 mo and required both CD4+ and CD8+ T cells. Mature tumor capturing DCs stimulated the differentiation of P1A tumor antigen-specific, CD8+ T cells and uniquely transferred tumor resistance to naive mice. Therefore, the access of dying tumor cells to DCs that are maturing to activated NKT cells efficiently induces long-lived adaptive resistance. JEM © The Rockefeller University Press. Fil:Idoyaga, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
If irradiated tumor cells could be rendered immunogenic, they would provide a safe, broad, and patient-specific array of antigens for immunotherapies. Prior approaches have emphasized genetic transduction of live tumor cells to express cytokines, costimulators, and surrogate foreign antigens. We asked if immunity could be achieved by delivering irradiated, major histocompatibility complex-negative plasmacytoma cells to maturing mouse dendritic cells (DCs) within lymphoid organs. Tumor cells injected intravenously (i.v.) were captured by splenic DCs, whereas subcutaneous (s.c.) injection led only to weak uptake in lymph node or spleen. The natural killer T (NKT) cells mobilizing glycolipid α-galactosyl ceramide, used to mature splenic DCs, served as an effective adjuvant to induce protective immunity. This adjuvant function was mimicked by a combination of poly IC and agonistic αCD40 antibody. The adjuvant glycolipid had to be coadministered with tumor cells i.v. rather than s.c. Specific resistance was generated both to a plasmacytoma and lymphoma. The resistance afforded by a single vaccination lasted >2 mo and required both CD4+ and CD8+ T cells. Mature tumor capturing DCs stimulated the differentiation of P1A tumor antigen-specific, CD8+ T cells and uniquely transferred tumor resistance to naive mice. Therefore, the access of dying tumor cells to DCs that are maturing to activated NKT cells efficiently induces long-lived adaptive resistance. JEM © The Rockefeller University Press. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_00221007_v202_n11_p1507_Liu |
url |
http://hdl.handle.net/20.500.12110/paper_00221007_v202_n11_p1507_Liu |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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reponame_str |
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Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
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ana@bl.fcen.uba.ar |
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