Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034

Autores
Ruiz Rodríguez, Luciana Gabriela; Aller, Kadri; Bru Chauve, Elena Magdalena; De Vuyst, Luc; Hebert, Elvira Maria; Mozzi, Fernanda Beatriz
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central composite design (CCD) as optimization strategy. The effect of the total saccharide (glucose + fructose, 1:2) content (TSC) in the medium (75, 100, 150, 200, and 225 g/l) and stirring (S; 50, 100, 200, 300 and 350 rpm) on mannitol production and yield by this strain was evaluated by using a 22 full-factorial CCD with 4 axial points (α = 1.5) and four replications of the center point, leading to 12 random experimental runs. Fermentations were carried out at 30 °C and pH 5.0 for 24 h. Minitab-15 software was used for experimental design and data analyses. The multiple response prediction analysis established 165 g/l of TSC and 200 rpm of S as optimal culture conditions to reach 85.03 g/l [95% CI (78.68, 91.39)] of mannitol and a yield of 82.02% [95% CI (71.98, 92.06)]. Finally, a validation experiment was conducted at the predicted optimum levels. The results obtained were 81.91 g/l of mannitol with a yield of 77.47% in outstanding agreement with the expected values. The mannitol 2-dehydrogenase enzyme activity was determined with 4.6–4.9 U/mg as the highest value found. To conclude, F. tropaeoli CRL 2034 produced high amounts of high-quality mannitol from fructose, being an excellent candidate for this polyol production.
Fil: Ruiz Rodríguez, Luciana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Aller, Kadri. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Center for Food and Fermentation Technologies; Estonia
Fil: Bru Chauve, Elena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: De Vuyst, Luc. Vrije Universiteit Brussel; Bélgica
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
CENTRAL COMPOSITE DESIGN
FRUCTOBACILLUS
LACTIC ACID BACTERIA
MANNITOL
RESPONSE SURFACE METHODOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/51413
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/51413
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034Ruiz Rodríguez, Luciana GabrielaAller, KadriBru Chauve, Elena MagdalenaDe Vuyst, LucHebert, Elvira MariaMozzi, Fernanda BeatrizCENTRAL COMPOSITE DESIGNFRUCTOBACILLUSLACTIC ACID BACTERIAMANNITOLRESPONSE SURFACE METHODOLOGYhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central composite design (CCD) as optimization strategy. The effect of the total saccharide (glucose + fructose, 1:2) content (TSC) in the medium (75, 100, 150, 200, and 225 g/l) and stirring (S; 50, 100, 200, 300 and 350 rpm) on mannitol production and yield by this strain was evaluated by using a 22 full-factorial CCD with 4 axial points (α = 1.5) and four replications of the center point, leading to 12 random experimental runs. Fermentations were carried out at 30 °C and pH 5.0 for 24 h. Minitab-15 software was used for experimental design and data analyses. The multiple response prediction analysis established 165 g/l of TSC and 200 rpm of S as optimal culture conditions to reach 85.03 g/l [95% CI (78.68, 91.39)] of mannitol and a yield of 82.02% [95% CI (71.98, 92.06)]. Finally, a validation experiment was conducted at the predicted optimum levels. The results obtained were 81.91 g/l of mannitol with a yield of 77.47% in outstanding agreement with the expected values. The mannitol 2-dehydrogenase enzyme activity was determined with 4.6–4.9 U/mg as the highest value found. To conclude, F. tropaeoli CRL 2034 produced high amounts of high-quality mannitol from fructose, being an excellent candidate for this polyol production.Fil: Ruiz Rodríguez, Luciana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Aller, Kadri. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Center for Food and Fermentation Technologies; EstoniaFil: Bru Chauve, Elena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: De Vuyst, Luc. Vrije Universiteit Brussel; BélgicaFil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaSpringer2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/51413Ruiz Rodríguez, Luciana Gabriela; Aller, Kadri; Bru Chauve, Elena Magdalena; De Vuyst, Luc; Hebert, Elvira Maria; et al.; Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034; Springer; Applied Microbiology and Biotechnology; 101; 15; 8-2017; 6165-61770175-7598CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-017-8395-1info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00253-017-8395-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:28:56Zoai:ri.conicet.gov.ar:11336/51413instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:28:56.774CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034
title Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034
spellingShingle Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034
Ruiz Rodríguez, Luciana Gabriela
CENTRAL COMPOSITE DESIGN
FRUCTOBACILLUS
LACTIC ACID BACTERIA
MANNITOL
RESPONSE SURFACE METHODOLOGY
title_short Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034
title_full Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034
title_fullStr Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034
title_full_unstemmed Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034
title_sort Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034
dc.creator.none.fl_str_mv Ruiz Rodríguez, Luciana Gabriela
Aller, Kadri
Bru Chauve, Elena Magdalena
De Vuyst, Luc
Hebert, Elvira Maria
Mozzi, Fernanda Beatriz
author Ruiz Rodríguez, Luciana Gabriela
author_facet Ruiz Rodríguez, Luciana Gabriela
Aller, Kadri
Bru Chauve, Elena Magdalena
De Vuyst, Luc
Hebert, Elvira Maria
Mozzi, Fernanda Beatriz
author_role author
author2 Aller, Kadri
Bru Chauve, Elena Magdalena
De Vuyst, Luc
Hebert, Elvira Maria
Mozzi, Fernanda Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CENTRAL COMPOSITE DESIGN
FRUCTOBACILLUS
LACTIC ACID BACTERIA
MANNITOL
RESPONSE SURFACE METHODOLOGY
topic CENTRAL COMPOSITE DESIGN
FRUCTOBACILLUS
LACTIC ACID BACTERIA
MANNITOL
RESPONSE SURFACE METHODOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central composite design (CCD) as optimization strategy. The effect of the total saccharide (glucose + fructose, 1:2) content (TSC) in the medium (75, 100, 150, 200, and 225 g/l) and stirring (S; 50, 100, 200, 300 and 350 rpm) on mannitol production and yield by this strain was evaluated by using a 22 full-factorial CCD with 4 axial points (α = 1.5) and four replications of the center point, leading to 12 random experimental runs. Fermentations were carried out at 30 °C and pH 5.0 for 24 h. Minitab-15 software was used for experimental design and data analyses. The multiple response prediction analysis established 165 g/l of TSC and 200 rpm of S as optimal culture conditions to reach 85.03 g/l [95% CI (78.68, 91.39)] of mannitol and a yield of 82.02% [95% CI (71.98, 92.06)]. Finally, a validation experiment was conducted at the predicted optimum levels. The results obtained were 81.91 g/l of mannitol with a yield of 77.47% in outstanding agreement with the expected values. The mannitol 2-dehydrogenase enzyme activity was determined with 4.6–4.9 U/mg as the highest value found. To conclude, F. tropaeoli CRL 2034 produced high amounts of high-quality mannitol from fructose, being an excellent candidate for this polyol production.
Fil: Ruiz Rodríguez, Luciana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Aller, Kadri. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Center for Food and Fermentation Technologies; Estonia
Fil: Bru Chauve, Elena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: De Vuyst, Luc. Vrije Universiteit Brussel; Bélgica
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central composite design (CCD) as optimization strategy. The effect of the total saccharide (glucose + fructose, 1:2) content (TSC) in the medium (75, 100, 150, 200, and 225 g/l) and stirring (S; 50, 100, 200, 300 and 350 rpm) on mannitol production and yield by this strain was evaluated by using a 22 full-factorial CCD with 4 axial points (α = 1.5) and four replications of the center point, leading to 12 random experimental runs. Fermentations were carried out at 30 °C and pH 5.0 for 24 h. Minitab-15 software was used for experimental design and data analyses. The multiple response prediction analysis established 165 g/l of TSC and 200 rpm of S as optimal culture conditions to reach 85.03 g/l [95% CI (78.68, 91.39)] of mannitol and a yield of 82.02% [95% CI (71.98, 92.06)]. Finally, a validation experiment was conducted at the predicted optimum levels. The results obtained were 81.91 g/l of mannitol with a yield of 77.47% in outstanding agreement with the expected values. The mannitol 2-dehydrogenase enzyme activity was determined with 4.6–4.9 U/mg as the highest value found. To conclude, F. tropaeoli CRL 2034 produced high amounts of high-quality mannitol from fructose, being an excellent candidate for this polyol production.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/51413
Ruiz Rodríguez, Luciana Gabriela; Aller, Kadri; Bru Chauve, Elena Magdalena; De Vuyst, Luc; Hebert, Elvira Maria; et al.; Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034; Springer; Applied Microbiology and Biotechnology; 101; 15; 8-2017; 6165-6177
0175-7598
CONICET Digital
CONICET
url http://hdl.handle.net/11336/51413
identifier_str_mv Ruiz Rodríguez, Luciana Gabriela; Aller, Kadri; Bru Chauve, Elena Magdalena; De Vuyst, Luc; Hebert, Elvira Maria; et al.; Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034; Springer; Applied Microbiology and Biotechnology; 101; 15; 8-2017; 6165-6177
0175-7598
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-017-8395-1
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00253-017-8395-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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