Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope

Autores
Dodes Traian, Martín Miguel; Gonzalez Flecha, Francisco Luis; Levi, Valeria
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lateral organization of biological membranes is frequently studied using fluorescence microscopy. One of the most widely used probes for these studies is laurdan. The fluorescence of this probe is sensitive to the environment polarity and thus laurdan reports the local penetration of water when inserted in membranes. Unfortunately, this probe can only be used under two-photon excitation due to its low photostability. This is a very important limitation since there are just a few laboratories with capability for two-photon microscopy. In this work, we explored the performance of C-laurdan, a carboxyl-modified version of laurdan, for imaging biological membranes using a conventional confocal microscopy setup. We acquired generalized polarization (GP) images of C-laurdan inserted in giant unillamelar vesicles composed of binary mixtures of lipids and verified that the probe allows observing the coexistence of different phases. We also tested the performance of the probe for measurement with living cells and registered GP images of melanophore cells labeled with C-laurdan in which we could observe highly-ordered regions such as filopodia. These findings show that C-laurdan can be successfully employed for studies of membrane lateral organization using a conventional confocal microscope and open the possibility of studying a wide variety of membrane-related processes.
Fil: Dodes Traian, Martín Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gonzalez Flecha, Francisco Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Fluorescence microscopy
Lipid rafts
Membranes
Membranes/Fluidity
Phospholipids
C-Laurdan
Generalized Polarization
Laurdan
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269065
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/269065
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscopeDodes Traian, Martín MiguelGonzalez Flecha, Francisco LuisLevi, ValeriaFluorescence microscopyLipid raftsMembranesMembranes/FluidityPhospholipidsC-LaurdanGeneralized PolarizationLaurdanhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lateral organization of biological membranes is frequently studied using fluorescence microscopy. One of the most widely used probes for these studies is laurdan. The fluorescence of this probe is sensitive to the environment polarity and thus laurdan reports the local penetration of water when inserted in membranes. Unfortunately, this probe can only be used under two-photon excitation due to its low photostability. This is a very important limitation since there are just a few laboratories with capability for two-photon microscopy. In this work, we explored the performance of C-laurdan, a carboxyl-modified version of laurdan, for imaging biological membranes using a conventional confocal microscopy setup. We acquired generalized polarization (GP) images of C-laurdan inserted in giant unillamelar vesicles composed of binary mixtures of lipids and verified that the probe allows observing the coexistence of different phases. We also tested the performance of the probe for measurement with living cells and registered GP images of melanophore cells labeled with C-laurdan in which we could observe highly-ordered regions such as filopodia. These findings show that C-laurdan can be successfully employed for studies of membrane lateral organization using a conventional confocal microscope and open the possibility of studying a wide variety of membrane-related processes.Fil: Dodes Traian, Martín Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Gonzalez Flecha, Francisco Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaAmerican Society for Biochemistry and Molecular Biology2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269065Dodes Traian, Martín Miguel; Gonzalez Flecha, Francisco Luis; Levi, Valeria; Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 53; 3; 1-2012; 609-6160022-22751539-7262CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.D021311info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:03Zoai:ri.conicet.gov.ar:11336/269065instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:03.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope
title Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope
spellingShingle Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope
Dodes Traian, Martín Miguel
Fluorescence microscopy
Lipid rafts
Membranes
Membranes/Fluidity
Phospholipids
C-Laurdan
Generalized Polarization
Laurdan
title_short Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope
title_full Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope
title_fullStr Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope
title_full_unstemmed Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope
title_sort Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope
dc.creator.none.fl_str_mv Dodes Traian, Martín Miguel
Gonzalez Flecha, Francisco Luis
Levi, Valeria
author Dodes Traian, Martín Miguel
author_facet Dodes Traian, Martín Miguel
Gonzalez Flecha, Francisco Luis
Levi, Valeria
author_role author
author2 Gonzalez Flecha, Francisco Luis
Levi, Valeria
author2_role author
author
dc.subject.none.fl_str_mv Fluorescence microscopy
Lipid rafts
Membranes
Membranes/Fluidity
Phospholipids
C-Laurdan
Generalized Polarization
Laurdan
topic Fluorescence microscopy
Lipid rafts
Membranes
Membranes/Fluidity
Phospholipids
C-Laurdan
Generalized Polarization
Laurdan
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lateral organization of biological membranes is frequently studied using fluorescence microscopy. One of the most widely used probes for these studies is laurdan. The fluorescence of this probe is sensitive to the environment polarity and thus laurdan reports the local penetration of water when inserted in membranes. Unfortunately, this probe can only be used under two-photon excitation due to its low photostability. This is a very important limitation since there are just a few laboratories with capability for two-photon microscopy. In this work, we explored the performance of C-laurdan, a carboxyl-modified version of laurdan, for imaging biological membranes using a conventional confocal microscopy setup. We acquired generalized polarization (GP) images of C-laurdan inserted in giant unillamelar vesicles composed of binary mixtures of lipids and verified that the probe allows observing the coexistence of different phases. We also tested the performance of the probe for measurement with living cells and registered GP images of melanophore cells labeled with C-laurdan in which we could observe highly-ordered regions such as filopodia. These findings show that C-laurdan can be successfully employed for studies of membrane lateral organization using a conventional confocal microscope and open the possibility of studying a wide variety of membrane-related processes.
Fil: Dodes Traian, Martín Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gonzalez Flecha, Francisco Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
description Lateral organization of biological membranes is frequently studied using fluorescence microscopy. One of the most widely used probes for these studies is laurdan. The fluorescence of this probe is sensitive to the environment polarity and thus laurdan reports the local penetration of water when inserted in membranes. Unfortunately, this probe can only be used under two-photon excitation due to its low photostability. This is a very important limitation since there are just a few laboratories with capability for two-photon microscopy. In this work, we explored the performance of C-laurdan, a carboxyl-modified version of laurdan, for imaging biological membranes using a conventional confocal microscopy setup. We acquired generalized polarization (GP) images of C-laurdan inserted in giant unillamelar vesicles composed of binary mixtures of lipids and verified that the probe allows observing the coexistence of different phases. We also tested the performance of the probe for measurement with living cells and registered GP images of melanophore cells labeled with C-laurdan in which we could observe highly-ordered regions such as filopodia. These findings show that C-laurdan can be successfully employed for studies of membrane lateral organization using a conventional confocal microscope and open the possibility of studying a wide variety of membrane-related processes.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269065
Dodes Traian, Martín Miguel; Gonzalez Flecha, Francisco Luis; Levi, Valeria; Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 53; 3; 1-2012; 609-616
0022-2275
1539-7262
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269065
identifier_str_mv Dodes Traian, Martín Miguel; Gonzalez Flecha, Francisco Luis; Levi, Valeria; Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research Papers In Press; 53; 3; 1-2012; 609-616
0022-2275
1539-7262
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.D021311
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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