Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury

Autores
Labombarda, Maria Florencia; Gonzalez, Susana Laura; Lima, Analia Ethel; Roig, Paulina; Guennoun, Rachida; Schumacher, Michael; de Nicola, Alejandro Federico
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Progesterone is emerging as a myelinizing factor for central nervous system injury. Successful remyelination requires proliferation and differentiation of oligodendrocyte precursor cells (OPC) into myelinating oligodendrocytes (OL), but this process is incomplete following injury. To study progesterone actions on remyelination, we administered progesterone (16 mg/kg/day) to rats with complete spinal cord injury. Rats were euthanized 3 or 21 days after steroid treatment. Short progesterone treatment: a) increased the number of OPC without effect on the injury-induced reduction of mature OL; b) increased mRNA and protein expression for the myelin basic protein (MBP) without effects on proteolipid protein (PLP) or myelin oligodendrocyte glycoprotein (MOG) and c) increased the mRNA for Olig2 and Nkx2.2 transcription factors involved in specification and differentiation of the OL lineage. Furthermore, long progesterone treatment: a) reduced OPC with a concomitant increase of OL; b) promoted differentiation of cells that incorporated bromodeoxyuridine, early after injury, into mature OL; c) increased mRNA and protein expression of PLP without effects on MBP or MOG and d) increased mRNA for the Olig1 transcription factor involved in myelin repair. These results suggest that early progesterone treatment enhanced the density of OPC and induced their differentiation into mature OL by increasing the expression of Olig2 and Nkx2.2. Twenty one days after injury, progesterone favors remyelination by increasing Olig1 (involved in repair of demyelinated lesions), PLP expression and enhancing OL maturation. Thus, progesterone effects on oligodendrogenesis and myelin proteins may constitute fundamental steps for repairing traumatic injury inflicted to the spinal cord.
Fil: Labombarda, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Gonzalez, Susana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Guennoun, Rachida. Inserm; Francia
Fil: Schumacher, Michael. Inserm; Francia
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Materia
Steroid
Trauma
Spinal Cord Injury
Progesterone
Remyelination
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24820
Acceder
id CONICETDig_7d30d994feed87b850f68ec83ad00e96
oai_identifier_str oai:ri.conicet.gov.ar:11336/24820
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injuryLabombarda, Maria FlorenciaGonzalez, Susana LauraLima, Analia EthelRoig, PaulinaGuennoun, RachidaSchumacher, Michaelde Nicola, Alejandro FedericoSteroidTraumaSpinal Cord InjuryProgesteroneRemyelinationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Progesterone is emerging as a myelinizing factor for central nervous system injury. Successful remyelination requires proliferation and differentiation of oligodendrocyte precursor cells (OPC) into myelinating oligodendrocytes (OL), but this process is incomplete following injury. To study progesterone actions on remyelination, we administered progesterone (16 mg/kg/day) to rats with complete spinal cord injury. Rats were euthanized 3 or 21 days after steroid treatment. Short progesterone treatment: a) increased the number of OPC without effect on the injury-induced reduction of mature OL; b) increased mRNA and protein expression for the myelin basic protein (MBP) without effects on proteolipid protein (PLP) or myelin oligodendrocyte glycoprotein (MOG) and c) increased the mRNA for Olig2 and Nkx2.2 transcription factors involved in specification and differentiation of the OL lineage. Furthermore, long progesterone treatment: a) reduced OPC with a concomitant increase of OL; b) promoted differentiation of cells that incorporated bromodeoxyuridine, early after injury, into mature OL; c) increased mRNA and protein expression of PLP without effects on MBP or MOG and d) increased mRNA for the Olig1 transcription factor involved in myelin repair. These results suggest that early progesterone treatment enhanced the density of OPC and induced their differentiation into mature OL by increasing the expression of Olig2 and Nkx2.2. Twenty one days after injury, progesterone favors remyelination by increasing Olig1 (involved in repair of demyelinated lesions), PLP expression and enhancing OL maturation. Thus, progesterone effects on oligodendrogenesis and myelin proteins may constitute fundamental steps for repairing traumatic injury inflicted to the spinal cord.Fil: Labombarda, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Gonzalez, Susana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Guennoun, Rachida. Inserm; FranciaFil: Schumacher, Michael. Inserm; FranciaFil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaWiley2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24820Labombarda, Maria Florencia; Gonzalez, Susana Laura; Lima, Analia Ethel; Roig, Paulina; Guennoun, Rachida; et al.; Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury; Wiley; Glia; 57; 8; -1-2009; 884-8970894-14911098-1136CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/glia.20814/fullinfo:eu-repo/semantics/altIdentifier/doi/10.1002/glia.20814info:eu-repo/semantics/altIdentifier/pmid/19053058info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:23Zoai:ri.conicet.gov.ar:11336/24820instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:24.895CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury
title Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury
spellingShingle Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury
Labombarda, Maria Florencia
Steroid
Trauma
Spinal Cord Injury
Progesterone
Remyelination
title_short Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury
title_full Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury
title_fullStr Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury
title_full_unstemmed Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury
title_sort Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury
dc.creator.none.fl_str_mv Labombarda, Maria Florencia
Gonzalez, Susana Laura
Lima, Analia Ethel
Roig, Paulina
Guennoun, Rachida
Schumacher, Michael
de Nicola, Alejandro Federico
author Labombarda, Maria Florencia
author_facet Labombarda, Maria Florencia
Gonzalez, Susana Laura
Lima, Analia Ethel
Roig, Paulina
Guennoun, Rachida
Schumacher, Michael
de Nicola, Alejandro Federico
author_role author
author2 Gonzalez, Susana Laura
Lima, Analia Ethel
Roig, Paulina
Guennoun, Rachida
Schumacher, Michael
de Nicola, Alejandro Federico
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Steroid
Trauma
Spinal Cord Injury
Progesterone
Remyelination
topic Steroid
Trauma
Spinal Cord Injury
Progesterone
Remyelination
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Progesterone is emerging as a myelinizing factor for central nervous system injury. Successful remyelination requires proliferation and differentiation of oligodendrocyte precursor cells (OPC) into myelinating oligodendrocytes (OL), but this process is incomplete following injury. To study progesterone actions on remyelination, we administered progesterone (16 mg/kg/day) to rats with complete spinal cord injury. Rats were euthanized 3 or 21 days after steroid treatment. Short progesterone treatment: a) increased the number of OPC without effect on the injury-induced reduction of mature OL; b) increased mRNA and protein expression for the myelin basic protein (MBP) without effects on proteolipid protein (PLP) or myelin oligodendrocyte glycoprotein (MOG) and c) increased the mRNA for Olig2 and Nkx2.2 transcription factors involved in specification and differentiation of the OL lineage. Furthermore, long progesterone treatment: a) reduced OPC with a concomitant increase of OL; b) promoted differentiation of cells that incorporated bromodeoxyuridine, early after injury, into mature OL; c) increased mRNA and protein expression of PLP without effects on MBP or MOG and d) increased mRNA for the Olig1 transcription factor involved in myelin repair. These results suggest that early progesterone treatment enhanced the density of OPC and induced their differentiation into mature OL by increasing the expression of Olig2 and Nkx2.2. Twenty one days after injury, progesterone favors remyelination by increasing Olig1 (involved in repair of demyelinated lesions), PLP expression and enhancing OL maturation. Thus, progesterone effects on oligodendrogenesis and myelin proteins may constitute fundamental steps for repairing traumatic injury inflicted to the spinal cord.
Fil: Labombarda, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Gonzalez, Susana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Guennoun, Rachida. Inserm; Francia
Fil: Schumacher, Michael. Inserm; Francia
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
description Progesterone is emerging as a myelinizing factor for central nervous system injury. Successful remyelination requires proliferation and differentiation of oligodendrocyte precursor cells (OPC) into myelinating oligodendrocytes (OL), but this process is incomplete following injury. To study progesterone actions on remyelination, we administered progesterone (16 mg/kg/day) to rats with complete spinal cord injury. Rats were euthanized 3 or 21 days after steroid treatment. Short progesterone treatment: a) increased the number of OPC without effect on the injury-induced reduction of mature OL; b) increased mRNA and protein expression for the myelin basic protein (MBP) without effects on proteolipid protein (PLP) or myelin oligodendrocyte glycoprotein (MOG) and c) increased the mRNA for Olig2 and Nkx2.2 transcription factors involved in specification and differentiation of the OL lineage. Furthermore, long progesterone treatment: a) reduced OPC with a concomitant increase of OL; b) promoted differentiation of cells that incorporated bromodeoxyuridine, early after injury, into mature OL; c) increased mRNA and protein expression of PLP without effects on MBP or MOG and d) increased mRNA for the Olig1 transcription factor involved in myelin repair. These results suggest that early progesterone treatment enhanced the density of OPC and induced their differentiation into mature OL by increasing the expression of Olig2 and Nkx2.2. Twenty one days after injury, progesterone favors remyelination by increasing Olig1 (involved in repair of demyelinated lesions), PLP expression and enhancing OL maturation. Thus, progesterone effects on oligodendrogenesis and myelin proteins may constitute fundamental steps for repairing traumatic injury inflicted to the spinal cord.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24820
Labombarda, Maria Florencia; Gonzalez, Susana Laura; Lima, Analia Ethel; Roig, Paulina; Guennoun, Rachida; et al.; Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury; Wiley; Glia; 57; 8; -1-2009; 884-897
0894-1491
1098-1136
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24820
identifier_str_mv Labombarda, Maria Florencia; Gonzalez, Susana Laura; Lima, Analia Ethel; Roig, Paulina; Guennoun, Rachida; et al.; Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury; Wiley; Glia; 57; 8; -1-2009; 884-897
0894-1491
1098-1136
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/glia.20814/full
info:eu-repo/semantics/altIdentifier/doi/10.1002/glia.20814
info:eu-repo/semantics/altIdentifier/pmid/19053058
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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