Single-molecule redox blinking of perylene diimide derivatives in water

Autores
Cordes, Thorben; Vogelsang, Jan; Anaya, Milena; Spagnuolo, Carla Cecilia; Gietl, Andreas; Summerer, Wolfram; Herrmann, Andreas; Müllen, Klaus; Tinnefeld, Philip
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dynamic developments in ultrasensitive and superresolution fluorescence microscopy call for improved fluorescence markers with increased photostability and new functionalities. We used single-molecule spectroscopy to study water-soluble perylene dicarboximide fluorophores (PDI), which were immobilized in aqueous buffer by attaching the fluorophore to DNA. Under these conditions bright fluorescence, comparable to that of single-molecule compatible organic fluorophores, is observed with homogeneous spectral and fluorescence decay time distributions. We additionally show how the fluorescence of the PDI can be controlled through photoinduced electron-transfer reactions by using different concentrations of reductants and oxidants, yielding either blinking or stable emission. We explain these properties by the redox potentials of PDI and the recently introduced ROXS (reducing and oxidizing system) concept. Finally, we evaluate how this fluorescence control of PDIs can be used for superresolution "Blink-Microscopy" in aqueous or organic media and more generally for single-molecule spectroscopy.
Fil: Cordes, Thorben. University of Oxford; Reino Unido. Ludwig Maximilians Universitat; Alemania
Fil: Vogelsang, Jan. Ludwig Maximilians Universitat; Alemania. University of Texas at Austin; Estados Unidos
Fil: Anaya, Milena. Max Planck Institute For Polymer Research; Alemania
Fil: Spagnuolo, Carla Cecilia. Max Planck Institute For Polymer Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gietl, Andreas. Ludwig Maximilians Universitat; Alemania
Fil: Summerer, Wolfram. Ludwig Maximilians Universitat; Alemania
Fil: Herrmann, Andreas. University of Groningen; Países Bajos
Fil: Müllen, Klaus. Max Planck Institute For Polymer Research; Alemania
Fil: Tinnefeld, Philip. Ludwig Maximilians Universitat; Alemania
Materia
Perylene Dyes
Single Molecule Spectroscopy
Photophysics
Superresolution
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68801
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/68801
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Single-molecule redox blinking of perylene diimide derivatives in waterCordes, ThorbenVogelsang, JanAnaya, MilenaSpagnuolo, Carla CeciliaGietl, AndreasSummerer, WolframHerrmann, AndreasMüllen, KlausTinnefeld, PhilipPerylene DyesSingle Molecule SpectroscopyPhotophysicsSuperresolutionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Dynamic developments in ultrasensitive and superresolution fluorescence microscopy call for improved fluorescence markers with increased photostability and new functionalities. We used single-molecule spectroscopy to study water-soluble perylene dicarboximide fluorophores (PDI), which were immobilized in aqueous buffer by attaching the fluorophore to DNA. Under these conditions bright fluorescence, comparable to that of single-molecule compatible organic fluorophores, is observed with homogeneous spectral and fluorescence decay time distributions. We additionally show how the fluorescence of the PDI can be controlled through photoinduced electron-transfer reactions by using different concentrations of reductants and oxidants, yielding either blinking or stable emission. We explain these properties by the redox potentials of PDI and the recently introduced ROXS (reducing and oxidizing system) concept. Finally, we evaluate how this fluorescence control of PDIs can be used for superresolution "Blink-Microscopy" in aqueous or organic media and more generally for single-molecule spectroscopy.Fil: Cordes, Thorben. University of Oxford; Reino Unido. Ludwig Maximilians Universitat; AlemaniaFil: Vogelsang, Jan. Ludwig Maximilians Universitat; Alemania. University of Texas at Austin; Estados UnidosFil: Anaya, Milena. Max Planck Institute For Polymer Research; AlemaniaFil: Spagnuolo, Carla Cecilia. Max Planck Institute For Polymer Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gietl, Andreas. Ludwig Maximilians Universitat; AlemaniaFil: Summerer, Wolfram. Ludwig Maximilians Universitat; AlemaniaFil: Herrmann, Andreas. University of Groningen; Países BajosFil: Müllen, Klaus. Max Planck Institute For Polymer Research; AlemaniaFil: Tinnefeld, Philip. Ludwig Maximilians Universitat; AlemaniaAmerican Chemical Society2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68801Cordes, Thorben; Vogelsang, Jan; Anaya, Milena; Spagnuolo, Carla Cecilia; Gietl, Andreas; et al.; Single-molecule redox blinking of perylene diimide derivatives in water; American Chemical Society; Journal of the American Chemical Society; 132; 7; 3-2010; 2404-24090002-7863CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/ja9099714info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/ja9099714info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:27Zoai:ri.conicet.gov.ar:11336/68801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:27.718CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Single-molecule redox blinking of perylene diimide derivatives in water
title Single-molecule redox blinking of perylene diimide derivatives in water
spellingShingle Single-molecule redox blinking of perylene diimide derivatives in water
Cordes, Thorben
Perylene Dyes
Single Molecule Spectroscopy
Photophysics
Superresolution
title_short Single-molecule redox blinking of perylene diimide derivatives in water
title_full Single-molecule redox blinking of perylene diimide derivatives in water
title_fullStr Single-molecule redox blinking of perylene diimide derivatives in water
title_full_unstemmed Single-molecule redox blinking of perylene diimide derivatives in water
title_sort Single-molecule redox blinking of perylene diimide derivatives in water
dc.creator.none.fl_str_mv Cordes, Thorben
Vogelsang, Jan
Anaya, Milena
Spagnuolo, Carla Cecilia
Gietl, Andreas
Summerer, Wolfram
Herrmann, Andreas
Müllen, Klaus
Tinnefeld, Philip
author Cordes, Thorben
author_facet Cordes, Thorben
Vogelsang, Jan
Anaya, Milena
Spagnuolo, Carla Cecilia
Gietl, Andreas
Summerer, Wolfram
Herrmann, Andreas
Müllen, Klaus
Tinnefeld, Philip
author_role author
author2 Vogelsang, Jan
Anaya, Milena
Spagnuolo, Carla Cecilia
Gietl, Andreas
Summerer, Wolfram
Herrmann, Andreas
Müllen, Klaus
Tinnefeld, Philip
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Perylene Dyes
Single Molecule Spectroscopy
Photophysics
Superresolution
topic Perylene Dyes
Single Molecule Spectroscopy
Photophysics
Superresolution
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dynamic developments in ultrasensitive and superresolution fluorescence microscopy call for improved fluorescence markers with increased photostability and new functionalities. We used single-molecule spectroscopy to study water-soluble perylene dicarboximide fluorophores (PDI), which were immobilized in aqueous buffer by attaching the fluorophore to DNA. Under these conditions bright fluorescence, comparable to that of single-molecule compatible organic fluorophores, is observed with homogeneous spectral and fluorescence decay time distributions. We additionally show how the fluorescence of the PDI can be controlled through photoinduced electron-transfer reactions by using different concentrations of reductants and oxidants, yielding either blinking or stable emission. We explain these properties by the redox potentials of PDI and the recently introduced ROXS (reducing and oxidizing system) concept. Finally, we evaluate how this fluorescence control of PDIs can be used for superresolution "Blink-Microscopy" in aqueous or organic media and more generally for single-molecule spectroscopy.
Fil: Cordes, Thorben. University of Oxford; Reino Unido. Ludwig Maximilians Universitat; Alemania
Fil: Vogelsang, Jan. Ludwig Maximilians Universitat; Alemania. University of Texas at Austin; Estados Unidos
Fil: Anaya, Milena. Max Planck Institute For Polymer Research; Alemania
Fil: Spagnuolo, Carla Cecilia. Max Planck Institute For Polymer Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gietl, Andreas. Ludwig Maximilians Universitat; Alemania
Fil: Summerer, Wolfram. Ludwig Maximilians Universitat; Alemania
Fil: Herrmann, Andreas. University of Groningen; Países Bajos
Fil: Müllen, Klaus. Max Planck Institute For Polymer Research; Alemania
Fil: Tinnefeld, Philip. Ludwig Maximilians Universitat; Alemania
description Dynamic developments in ultrasensitive and superresolution fluorescence microscopy call for improved fluorescence markers with increased photostability and new functionalities. We used single-molecule spectroscopy to study water-soluble perylene dicarboximide fluorophores (PDI), which were immobilized in aqueous buffer by attaching the fluorophore to DNA. Under these conditions bright fluorescence, comparable to that of single-molecule compatible organic fluorophores, is observed with homogeneous spectral and fluorescence decay time distributions. We additionally show how the fluorescence of the PDI can be controlled through photoinduced electron-transfer reactions by using different concentrations of reductants and oxidants, yielding either blinking or stable emission. We explain these properties by the redox potentials of PDI and the recently introduced ROXS (reducing and oxidizing system) concept. Finally, we evaluate how this fluorescence control of PDIs can be used for superresolution "Blink-Microscopy" in aqueous or organic media and more generally for single-molecule spectroscopy.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68801
Cordes, Thorben; Vogelsang, Jan; Anaya, Milena; Spagnuolo, Carla Cecilia; Gietl, Andreas; et al.; Single-molecule redox blinking of perylene diimide derivatives in water; American Chemical Society; Journal of the American Chemical Society; 132; 7; 3-2010; 2404-2409
0002-7863
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68801
identifier_str_mv Cordes, Thorben; Vogelsang, Jan; Anaya, Milena; Spagnuolo, Carla Cecilia; Gietl, Andreas; et al.; Single-molecule redox blinking of perylene diimide derivatives in water; American Chemical Society; Journal of the American Chemical Society; 132; 7; 3-2010; 2404-2409
0002-7863
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/ja9099714
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/ja9099714
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269959616462848
score 13.13397

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