Shifting molecular localization by plasmonic coupling in a single-molecule mirage

Autores
Raab, Mario; Vietz, Carolin; Stefani, Fernando Daniel; Acuna, Guillermo Pedro; Tinnefeld, Philip
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Over the last decade, two fields have dominated the attention of sub-diffraction photonics research: Plasmonics and fluorescence nanoscopy. Nanoscopy based on single-molecule localization offers a practical way to explore plasmonic interactions with nanometre resolution. However, this seemingly straightforward technique may retrieve false positional information. Here, we make use of the DNA origami technique to both control a nanometric separation between emitters and a gold nanoparticle, and as a platform for super-resolution imaging based on single-molecule localization. This enables a quantitative comparison between the position retrieved from single-molecule localization, the true position of the emitter and full-field simulations. We demonstrate that plasmonic coupling leads to shifted molecular localizations of up to 30 nm: A single-molecule mirage.
Fil: Raab, Mario. Technical University Of Braunschweig; Alemania
Fil: Vietz, Carolin. Technical University of Braunschweig; Alemania; Argentina
Fil: Stefani, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Acuna, Guillermo Pedro. Technical University Of Braunschweig; Alemania
Fil: Tinnefeld, Philip. Technical University Of Braunschweig; Alemania
Materia
PLASMON
SINGLE MOLECULE
SUPERRESOLUTION
DNA ORIGAMI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87554
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Shifting molecular localization by plasmonic coupling in a single-molecule mirageRaab, MarioVietz, CarolinStefani, Fernando DanielAcuna, Guillermo PedroTinnefeld, PhilipPLASMONSINGLE MOLECULESUPERRESOLUTIONDNA ORIGAMIhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Over the last decade, two fields have dominated the attention of sub-diffraction photonics research: Plasmonics and fluorescence nanoscopy. Nanoscopy based on single-molecule localization offers a practical way to explore plasmonic interactions with nanometre resolution. However, this seemingly straightforward technique may retrieve false positional information. Here, we make use of the DNA origami technique to both control a nanometric separation between emitters and a gold nanoparticle, and as a platform for super-resolution imaging based on single-molecule localization. This enables a quantitative comparison between the position retrieved from single-molecule localization, the true position of the emitter and full-field simulations. We demonstrate that plasmonic coupling leads to shifted molecular localizations of up to 30 nm: A single-molecule mirage.Fil: Raab, Mario. Technical University Of Braunschweig; AlemaniaFil: Vietz, Carolin. Technical University of Braunschweig; Alemania; ArgentinaFil: Stefani, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Acuna, Guillermo Pedro. Technical University Of Braunschweig; AlemaniaFil: Tinnefeld, Philip. Technical University Of Braunschweig; AlemaniaNature Publishing Group2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87554Raab, Mario; Vietz, Carolin; Stefani, Fernando Daniel; Acuna, Guillermo Pedro; Tinnefeld, Philip; Shifting molecular localization by plasmonic coupling in a single-molecule mirage; Nature Publishing Group; Nature Communications; 8; 1-2017; 1-5; 139662041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms13966info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms13966info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:38Zoai:ri.conicet.gov.ar:11336/87554instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:38.778CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Shifting molecular localization by plasmonic coupling in a single-molecule mirage
title Shifting molecular localization by plasmonic coupling in a single-molecule mirage
spellingShingle Shifting molecular localization by plasmonic coupling in a single-molecule mirage
Raab, Mario
PLASMON
SINGLE MOLECULE
SUPERRESOLUTION
DNA ORIGAMI
title_short Shifting molecular localization by plasmonic coupling in a single-molecule mirage
title_full Shifting molecular localization by plasmonic coupling in a single-molecule mirage
title_fullStr Shifting molecular localization by plasmonic coupling in a single-molecule mirage
title_full_unstemmed Shifting molecular localization by plasmonic coupling in a single-molecule mirage
title_sort Shifting molecular localization by plasmonic coupling in a single-molecule mirage
dc.creator.none.fl_str_mv Raab, Mario
Vietz, Carolin
Stefani, Fernando Daniel
Acuna, Guillermo Pedro
Tinnefeld, Philip
author Raab, Mario
author_facet Raab, Mario
Vietz, Carolin
Stefani, Fernando Daniel
Acuna, Guillermo Pedro
Tinnefeld, Philip
author_role author
author2 Vietz, Carolin
Stefani, Fernando Daniel
Acuna, Guillermo Pedro
Tinnefeld, Philip
author2_role author
author
author
author
dc.subject.none.fl_str_mv PLASMON
SINGLE MOLECULE
SUPERRESOLUTION
DNA ORIGAMI
topic PLASMON
SINGLE MOLECULE
SUPERRESOLUTION
DNA ORIGAMI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Over the last decade, two fields have dominated the attention of sub-diffraction photonics research: Plasmonics and fluorescence nanoscopy. Nanoscopy based on single-molecule localization offers a practical way to explore plasmonic interactions with nanometre resolution. However, this seemingly straightforward technique may retrieve false positional information. Here, we make use of the DNA origami technique to both control a nanometric separation between emitters and a gold nanoparticle, and as a platform for super-resolution imaging based on single-molecule localization. This enables a quantitative comparison between the position retrieved from single-molecule localization, the true position of the emitter and full-field simulations. We demonstrate that plasmonic coupling leads to shifted molecular localizations of up to 30 nm: A single-molecule mirage.
Fil: Raab, Mario. Technical University Of Braunschweig; Alemania
Fil: Vietz, Carolin. Technical University of Braunschweig; Alemania; Argentina
Fil: Stefani, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Acuna, Guillermo Pedro. Technical University Of Braunschweig; Alemania
Fil: Tinnefeld, Philip. Technical University Of Braunschweig; Alemania
description Over the last decade, two fields have dominated the attention of sub-diffraction photonics research: Plasmonics and fluorescence nanoscopy. Nanoscopy based on single-molecule localization offers a practical way to explore plasmonic interactions with nanometre resolution. However, this seemingly straightforward technique may retrieve false positional information. Here, we make use of the DNA origami technique to both control a nanometric separation between emitters and a gold nanoparticle, and as a platform for super-resolution imaging based on single-molecule localization. This enables a quantitative comparison between the position retrieved from single-molecule localization, the true position of the emitter and full-field simulations. We demonstrate that plasmonic coupling leads to shifted molecular localizations of up to 30 nm: A single-molecule mirage.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87554
Raab, Mario; Vietz, Carolin; Stefani, Fernando Daniel; Acuna, Guillermo Pedro; Tinnefeld, Philip; Shifting molecular localization by plasmonic coupling in a single-molecule mirage; Nature Publishing Group; Nature Communications; 8; 1-2017; 1-5; 13966
2041-1723
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87554
identifier_str_mv Raab, Mario; Vietz, Carolin; Stefani, Fernando Daniel; Acuna, Guillermo Pedro; Tinnefeld, Philip; Shifting molecular localization by plasmonic coupling in a single-molecule mirage; Nature Publishing Group; Nature Communications; 8; 1-2017; 1-5; 13966
2041-1723
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms13966
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms13966
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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