The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition

Autores
Bruna, Flavia Alejandra; Fernández, Katia; Urrejola, Felipe; Touma, Jorge; Navarro, Myriam; Sepúlveda, Betsabet; Larrazabal Fuentes, María; Paredes, Adrián; Neira, Iván; Ferrando, Matías; Osorio, José Manuel; Yañez, Osvaldo; Bravo, Jessica
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Mapuche and their ancestors have used D. winteri in traditional medicine. In the present study, the essential oil extract of D. winteri leaves (DW_EO) were characterized chemically and biologically to evaluate its pharmacological activity. In vitro antioxidant activity was assayed, and antitumor activity was evaluated in non-tumor and tumor-cell culture lines. Caenorhabditis elegans was used as a model to evaluate toxicity, and the chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The chemical oil composition was characterized principally of five major terpenes: 4 sesquiterpenes γ-Eudesmol (39.7%), β-Caryophyllene (33.7%), Elemol (25.9%), α-Eudesmol (0.3%) and 1 diterpene Kaunene (0.4%). By quantum calculations, it was determined that all oils have the ability to capture and yield electrons, which is consistent with the moderate antioxidant activity of DW_EO detected in vitro. Furthermore, by molecular docking is estimated that these oils can bind to proteins involved in the production of oxygen radicals. Of these proteins, CYP2C9 could bind energetically, reaching binding energy between −6.8 and −9.2 kCal/mol for the 5 terpenes studied, highlighting among these β-Caryophyllen and γ-Eudesmol. DW_EO has effect against H. pylori (MIC 32 μg/ml), S. aureus (MIC 8 μg/ml), E. coli (MIC 32 μg/ml) and C. albicans (MIC 64 μg/ml), β-Caryophyllen and γ -Eudesmol (MIC 64 μg/ml) and could selectively inhibit the proliferation of epithelial tumor cell lines but showed low against C. elegans (0.39–1.56 μg mL−1). Therefore, DW_EO may be used as a source of bioactive compounds in novel pharmacological treatments for medical application, agronomics, sanitation, and food.
Fil: Bruna, Flavia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Diego Portales; Chile
Fil: Fernández, Katia. Universidad Diego Portales; Chile
Fil: Urrejola, Felipe. Universidad Diego Portales; Chile
Fil: Touma, Jorge. Universidad Diego Portales; Chile
Fil: Navarro, Myriam. Universidad Diego Portales; Chile
Fil: Sepúlveda, Betsabet. Universidad de Chile; Chile
Fil: Larrazabal Fuentes, María. Universidad de Antofagasta (uantof);
Fil: Paredes, Adrián. Universidad de Antofagasta (uantof);
Fil: Neira, Iván. Universidad de Antofagasta (uantof);
Fil: Ferrando, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Osorio, José Manuel. Universidad Diego Portales; Chile. Universidad Andrés Bello; Chile
Fil: Yañez, Osvaldo. Universidad de Las Américas.; Ecuador. Center of New Drugs for Hypertension; Chile
Fil: Bravo, Jessica. Universidad Diego Portales; Chile
Materia
ESSENTIAL OIL,
ANTIOXIDANT ACTIVITY
CITOTOXICITY ACTIVITY
ANTIMICROBIAL ACTIVITY
ANTITUMORAL ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/203452
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/203452
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical compositionBruna, Flavia AlejandraFernández, KatiaUrrejola, FelipeTouma, JorgeNavarro, MyriamSepúlveda, BetsabetLarrazabal Fuentes, MaríaParedes, AdriánNeira, IvánFerrando, MatíasOsorio, José ManuelYañez, OsvaldoBravo, JessicaESSENTIAL OIL,ANTIOXIDANT ACTIVITYCITOTOXICITY ACTIVITYANTIMICROBIAL ACTIVITYANTITUMORAL ACTIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Mapuche and their ancestors have used D. winteri in traditional medicine. In the present study, the essential oil extract of D. winteri leaves (DW_EO) were characterized chemically and biologically to evaluate its pharmacological activity. In vitro antioxidant activity was assayed, and antitumor activity was evaluated in non-tumor and tumor-cell culture lines. Caenorhabditis elegans was used as a model to evaluate toxicity, and the chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The chemical oil composition was characterized principally of five major terpenes: 4 sesquiterpenes γ-Eudesmol (39.7%), β-Caryophyllene (33.7%), Elemol (25.9%), α-Eudesmol (0.3%) and 1 diterpene Kaunene (0.4%). By quantum calculations, it was determined that all oils have the ability to capture and yield electrons, which is consistent with the moderate antioxidant activity of DW_EO detected in vitro. Furthermore, by molecular docking is estimated that these oils can bind to proteins involved in the production of oxygen radicals. Of these proteins, CYP2C9 could bind energetically, reaching binding energy between −6.8 and −9.2 kCal/mol for the 5 terpenes studied, highlighting among these β-Caryophyllen and γ-Eudesmol. DW_EO has effect against H. pylori (MIC 32 μg/ml), S. aureus (MIC 8 μg/ml), E. coli (MIC 32 μg/ml) and C. albicans (MIC 64 μg/ml), β-Caryophyllen and γ -Eudesmol (MIC 64 μg/ml) and could selectively inhibit the proliferation of epithelial tumor cell lines but showed low against C. elegans (0.39–1.56 μg mL−1). Therefore, DW_EO may be used as a source of bioactive compounds in novel pharmacological treatments for medical application, agronomics, sanitation, and food.Fil: Bruna, Flavia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Diego Portales; ChileFil: Fernández, Katia. Universidad Diego Portales; ChileFil: Urrejola, Felipe. Universidad Diego Portales; ChileFil: Touma, Jorge. Universidad Diego Portales; ChileFil: Navarro, Myriam. Universidad Diego Portales; ChileFil: Sepúlveda, Betsabet. Universidad de Chile; ChileFil: Larrazabal Fuentes, María. Universidad de Antofagasta (uantof);Fil: Paredes, Adrián. Universidad de Antofagasta (uantof);Fil: Neira, Iván. Universidad de Antofagasta (uantof);Fil: Ferrando, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Osorio, José Manuel. Universidad Diego Portales; Chile. Universidad Andrés Bello; ChileFil: Yañez, Osvaldo. Universidad de Las Américas.; Ecuador. Center of New Drugs for Hypertension; ChileFil: Bravo, Jessica. Universidad Diego Portales; ChileFrontiers Media2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/203452Bruna, Flavia Alejandra; Fernández, Katia; Urrejola, Felipe; Touma, Jorge; Navarro, Myriam; et al.; The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition; Frontiers Media; Frontiers in Natural Products; 1; 11-2022; 1-162813-2602CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fntpr.2022.958425/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fntpr.2022.958425info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:51Zoai:ri.conicet.gov.ar:11336/203452instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:51.578CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition
title The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition
spellingShingle The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition
Bruna, Flavia Alejandra
ESSENTIAL OIL,
ANTIOXIDANT ACTIVITY
CITOTOXICITY ACTIVITY
ANTIMICROBIAL ACTIVITY
ANTITUMORAL ACTIVITY
title_short The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition
title_full The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition
title_fullStr The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition
title_full_unstemmed The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition
title_sort The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition
dc.creator.none.fl_str_mv Bruna, Flavia Alejandra
Fernández, Katia
Urrejola, Felipe
Touma, Jorge
Navarro, Myriam
Sepúlveda, Betsabet
Larrazabal Fuentes, María
Paredes, Adrián
Neira, Iván
Ferrando, Matías
Osorio, José Manuel
Yañez, Osvaldo
Bravo, Jessica
author Bruna, Flavia Alejandra
author_facet Bruna, Flavia Alejandra
Fernández, Katia
Urrejola, Felipe
Touma, Jorge
Navarro, Myriam
Sepúlveda, Betsabet
Larrazabal Fuentes, María
Paredes, Adrián
Neira, Iván
Ferrando, Matías
Osorio, José Manuel
Yañez, Osvaldo
Bravo, Jessica
author_role author
author2 Fernández, Katia
Urrejola, Felipe
Touma, Jorge
Navarro, Myriam
Sepúlveda, Betsabet
Larrazabal Fuentes, María
Paredes, Adrián
Neira, Iván
Ferrando, Matías
Osorio, José Manuel
Yañez, Osvaldo
Bravo, Jessica
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ESSENTIAL OIL,
ANTIOXIDANT ACTIVITY
CITOTOXICITY ACTIVITY
ANTIMICROBIAL ACTIVITY
ANTITUMORAL ACTIVITY
topic ESSENTIAL OIL,
ANTIOXIDANT ACTIVITY
CITOTOXICITY ACTIVITY
ANTIMICROBIAL ACTIVITY
ANTITUMORAL ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Mapuche and their ancestors have used D. winteri in traditional medicine. In the present study, the essential oil extract of D. winteri leaves (DW_EO) were characterized chemically and biologically to evaluate its pharmacological activity. In vitro antioxidant activity was assayed, and antitumor activity was evaluated in non-tumor and tumor-cell culture lines. Caenorhabditis elegans was used as a model to evaluate toxicity, and the chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The chemical oil composition was characterized principally of five major terpenes: 4 sesquiterpenes γ-Eudesmol (39.7%), β-Caryophyllene (33.7%), Elemol (25.9%), α-Eudesmol (0.3%) and 1 diterpene Kaunene (0.4%). By quantum calculations, it was determined that all oils have the ability to capture and yield electrons, which is consistent with the moderate antioxidant activity of DW_EO detected in vitro. Furthermore, by molecular docking is estimated that these oils can bind to proteins involved in the production of oxygen radicals. Of these proteins, CYP2C9 could bind energetically, reaching binding energy between −6.8 and −9.2 kCal/mol for the 5 terpenes studied, highlighting among these β-Caryophyllen and γ-Eudesmol. DW_EO has effect against H. pylori (MIC 32 μg/ml), S. aureus (MIC 8 μg/ml), E. coli (MIC 32 μg/ml) and C. albicans (MIC 64 μg/ml), β-Caryophyllen and γ -Eudesmol (MIC 64 μg/ml) and could selectively inhibit the proliferation of epithelial tumor cell lines but showed low against C. elegans (0.39–1.56 μg mL−1). Therefore, DW_EO may be used as a source of bioactive compounds in novel pharmacological treatments for medical application, agronomics, sanitation, and food.
Fil: Bruna, Flavia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Diego Portales; Chile
Fil: Fernández, Katia. Universidad Diego Portales; Chile
Fil: Urrejola, Felipe. Universidad Diego Portales; Chile
Fil: Touma, Jorge. Universidad Diego Portales; Chile
Fil: Navarro, Myriam. Universidad Diego Portales; Chile
Fil: Sepúlveda, Betsabet. Universidad de Chile; Chile
Fil: Larrazabal Fuentes, María. Universidad de Antofagasta (uantof);
Fil: Paredes, Adrián. Universidad de Antofagasta (uantof);
Fil: Neira, Iván. Universidad de Antofagasta (uantof);
Fil: Ferrando, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Osorio, José Manuel. Universidad Diego Portales; Chile. Universidad Andrés Bello; Chile
Fil: Yañez, Osvaldo. Universidad de Las Américas.; Ecuador. Center of New Drugs for Hypertension; Chile
Fil: Bravo, Jessica. Universidad Diego Portales; Chile
description The Mapuche and their ancestors have used D. winteri in traditional medicine. In the present study, the essential oil extract of D. winteri leaves (DW_EO) were characterized chemically and biologically to evaluate its pharmacological activity. In vitro antioxidant activity was assayed, and antitumor activity was evaluated in non-tumor and tumor-cell culture lines. Caenorhabditis elegans was used as a model to evaluate toxicity, and the chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The chemical oil composition was characterized principally of five major terpenes: 4 sesquiterpenes γ-Eudesmol (39.7%), β-Caryophyllene (33.7%), Elemol (25.9%), α-Eudesmol (0.3%) and 1 diterpene Kaunene (0.4%). By quantum calculations, it was determined that all oils have the ability to capture and yield electrons, which is consistent with the moderate antioxidant activity of DW_EO detected in vitro. Furthermore, by molecular docking is estimated that these oils can bind to proteins involved in the production of oxygen radicals. Of these proteins, CYP2C9 could bind energetically, reaching binding energy between −6.8 and −9.2 kCal/mol for the 5 terpenes studied, highlighting among these β-Caryophyllen and γ-Eudesmol. DW_EO has effect against H. pylori (MIC 32 μg/ml), S. aureus (MIC 8 μg/ml), E. coli (MIC 32 μg/ml) and C. albicans (MIC 64 μg/ml), β-Caryophyllen and γ -Eudesmol (MIC 64 μg/ml) and could selectively inhibit the proliferation of epithelial tumor cell lines but showed low against C. elegans (0.39–1.56 μg mL−1). Therefore, DW_EO may be used as a source of bioactive compounds in novel pharmacological treatments for medical application, agronomics, sanitation, and food.
publishDate 2022
dc.date.none.fl_str_mv 2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/203452
Bruna, Flavia Alejandra; Fernández, Katia; Urrejola, Felipe; Touma, Jorge; Navarro, Myriam; et al.; The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition; Frontiers Media; Frontiers in Natural Products; 1; 11-2022; 1-16
2813-2602
CONICET Digital
CONICET
url http://hdl.handle.net/11336/203452
identifier_str_mv Bruna, Flavia Alejandra; Fernández, Katia; Urrejola, Felipe; Touma, Jorge; Navarro, Myriam; et al.; The essential oil from Drimys winteri possess activity: Antioxidant, theoretical chemistry reactivity, antimicrobial, antiproliferative and chemical composition; Frontiers Media; Frontiers in Natural Products; 1; 11-2022; 1-16
2813-2602
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fntpr.2022.958425/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fntpr.2022.958425
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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