Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development

Autores
Cohen, Stefanía; Diaz, Marina Vera; Díaz, Alcira Ofelia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, histological and histochemical features of the larval digestive system of Argentine anchovy Engraulis anchoita were described. Structural changes during ontogenetic development were also characterized, and comparisons between the beginning and the end of larval development were made. Histological sections of larvae were subjected to histochemical and routine histological techniques to localize and differentiate glycoproteins (GPs). Both at an early and a late larval stage, the oesophageal goblet cells reacted more intensely than those of the rest of the digestive tract, and only the oesophagus exhibited metachromasia with toluidine blue techniques at different pHs, thus revealing diverse GPs at different concentrations. The GPs histochemical composition in the intestine varied with the developmental stage and the intestinal zone. The absence of goblet cells characterized the foregut; however, they started differentiation at an advanced stage in the midgut. These cells could be detected in the hindgut both at the beginning and at the end of development. The attached glands showed a varied glycoprotein composition. The digestive tract of E. anchoita presented a high level of complexity, related to the multiple functions of mucus in the digestive tract, such as lubrication, protection, antimicrobial function and ionic and osmotic regulation.
Fil: Cohen, Stefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Diaz, Marina Vera. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Díaz, Alcira Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
DIGESTIVE SYSTEM
ENGRAULIS ANCHOITA
GLYCOPROTEINS
HISTOCHEMISTRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180563
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/180563
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic developmentCohen, StefaníaDiaz, Marina VeraDíaz, Alcira OfeliaDIGESTIVE SYSTEMENGRAULIS ANCHOITAGLYCOPROTEINSHISTOCHEMISTRYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this work, histological and histochemical features of the larval digestive system of Argentine anchovy Engraulis anchoita were described. Structural changes during ontogenetic development were also characterized, and comparisons between the beginning and the end of larval development were made. Histological sections of larvae were subjected to histochemical and routine histological techniques to localize and differentiate glycoproteins (GPs). Both at an early and a late larval stage, the oesophageal goblet cells reacted more intensely than those of the rest of the digestive tract, and only the oesophagus exhibited metachromasia with toluidine blue techniques at different pHs, thus revealing diverse GPs at different concentrations. The GPs histochemical composition in the intestine varied with the developmental stage and the intestinal zone. The absence of goblet cells characterized the foregut; however, they started differentiation at an advanced stage in the midgut. These cells could be detected in the hindgut both at the beginning and at the end of development. The attached glands showed a varied glycoprotein composition. The digestive tract of E. anchoita presented a high level of complexity, related to the multiple functions of mucus in the digestive tract, such as lubrication, protection, antimicrobial function and ionic and osmotic regulation.Fil: Cohen, Stefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Diaz, Marina Vera. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Díaz, Alcira Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaWiley Blackwell Publishing, Inc2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180563Cohen, Stefanía; Diaz, Marina Vera; Díaz, Alcira Ofelia; Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development; Wiley Blackwell Publishing, Inc; Acta Zoologica; 95; 4; 6-2013; 409-4200001-7272CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/azo.12038info:eu-repo/semantics/altIdentifier/doi/10.1111/azo.12038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:15Zoai:ri.conicet.gov.ar:11336/180563instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:15.963CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development
title Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development
spellingShingle Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development
Cohen, Stefanía
DIGESTIVE SYSTEM
ENGRAULIS ANCHOITA
GLYCOPROTEINS
HISTOCHEMISTRY
title_short Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development
title_full Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development
title_fullStr Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development
title_full_unstemmed Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development
title_sort Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development
dc.creator.none.fl_str_mv Cohen, Stefanía
Diaz, Marina Vera
Díaz, Alcira Ofelia
author Cohen, Stefanía
author_facet Cohen, Stefanía
Diaz, Marina Vera
Díaz, Alcira Ofelia
author_role author
author2 Diaz, Marina Vera
Díaz, Alcira Ofelia
author2_role author
author
dc.subject.none.fl_str_mv DIGESTIVE SYSTEM
ENGRAULIS ANCHOITA
GLYCOPROTEINS
HISTOCHEMISTRY
topic DIGESTIVE SYSTEM
ENGRAULIS ANCHOITA
GLYCOPROTEINS
HISTOCHEMISTRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, histological and histochemical features of the larval digestive system of Argentine anchovy Engraulis anchoita were described. Structural changes during ontogenetic development were also characterized, and comparisons between the beginning and the end of larval development were made. Histological sections of larvae were subjected to histochemical and routine histological techniques to localize and differentiate glycoproteins (GPs). Both at an early and a late larval stage, the oesophageal goblet cells reacted more intensely than those of the rest of the digestive tract, and only the oesophagus exhibited metachromasia with toluidine blue techniques at different pHs, thus revealing diverse GPs at different concentrations. The GPs histochemical composition in the intestine varied with the developmental stage and the intestinal zone. The absence of goblet cells characterized the foregut; however, they started differentiation at an advanced stage in the midgut. These cells could be detected in the hindgut both at the beginning and at the end of development. The attached glands showed a varied glycoprotein composition. The digestive tract of E. anchoita presented a high level of complexity, related to the multiple functions of mucus in the digestive tract, such as lubrication, protection, antimicrobial function and ionic and osmotic regulation.
Fil: Cohen, Stefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Diaz, Marina Vera. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Díaz, Alcira Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description In this work, histological and histochemical features of the larval digestive system of Argentine anchovy Engraulis anchoita were described. Structural changes during ontogenetic development were also characterized, and comparisons between the beginning and the end of larval development were made. Histological sections of larvae were subjected to histochemical and routine histological techniques to localize and differentiate glycoproteins (GPs). Both at an early and a late larval stage, the oesophageal goblet cells reacted more intensely than those of the rest of the digestive tract, and only the oesophagus exhibited metachromasia with toluidine blue techniques at different pHs, thus revealing diverse GPs at different concentrations. The GPs histochemical composition in the intestine varied with the developmental stage and the intestinal zone. The absence of goblet cells characterized the foregut; however, they started differentiation at an advanced stage in the midgut. These cells could be detected in the hindgut both at the beginning and at the end of development. The attached glands showed a varied glycoprotein composition. The digestive tract of E. anchoita presented a high level of complexity, related to the multiple functions of mucus in the digestive tract, such as lubrication, protection, antimicrobial function and ionic and osmotic regulation.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180563
Cohen, Stefanía; Diaz, Marina Vera; Díaz, Alcira Ofelia; Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development; Wiley Blackwell Publishing, Inc; Acta Zoologica; 95; 4; 6-2013; 409-420
0001-7272
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180563
identifier_str_mv Cohen, Stefanía; Diaz, Marina Vera; Díaz, Alcira Ofelia; Histological and histochemical study of the digestive system of the Argentine anchovy larvae (Engraulis anchoita) at different developmental stages of their ontogenetic development; Wiley Blackwell Publishing, Inc; Acta Zoologica; 95; 4; 6-2013; 409-420
0001-7272
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/azo.12038
info:eu-repo/semantics/altIdentifier/doi/10.1111/azo.12038
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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