Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization

Autores
Lamas, Daniela Lorena; Yeannes, Maria Isabel; Massa, Agueda Elena
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Marine by-products contain valuable protein fractions. To ensure a profitable utilization of wastes from the fish industry, the knowledge about their quality and composition is necessary. Fish digestive proteolytic enzymes from cold-adapted ectothermic organisms have found applications in several industries because their temperature requirements and other characteristics differ from those of homologous proteases from warm-blooded animals. Herein, we describe detection, partial purification, and characterization of proteolytic enzymes from the viscera and heads of Engraulis anchoita. Enzymatic activities of the by-products were assayed using azocaseín as a substrate. To characterize the alkali protein fraction, the activity against inhibitors and their molecular weights were studied. The crude protein extract exhibited maximal activity at pH 8.0 and 60EC. Results relative to the substrate-specific Nabenzoyl-DL-arginine-p-nitroanilide and the 25 kDa molecular weight indicated that the recovered protease was trypsin. The activity showed an increment in presence of SDS and a slight decrease when it was incubated with EDTA. Increasing the concentration of NaCl up to 5% did not significantly decrease the protein’s activity. The results obtained suggest that by-products of anchovy industry could be used in the detergents industry.
Fil: Lamas, Daniela Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Subsede Instituto Nacional de Investigación y Desarrollo Pesquero; Argentina
Fil: Yeannes, Maria Isabel. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Massa, Agueda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Subsede Instituto Nacional de Investigación y Desarrollo Pesquero; Argentina
Materia
ALKALINE PROTEASES
ENGRAULIS ANCHOITA
HEADS
TRYPSIN
VISCERA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78152
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/78152
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterizationLamas, Daniela LorenaYeannes, Maria IsabelMassa, Agueda ElenaALKALINE PROTEASESENGRAULIS ANCHOITAHEADSTRYPSINVISCERAhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Marine by-products contain valuable protein fractions. To ensure a profitable utilization of wastes from the fish industry, the knowledge about their quality and composition is necessary. Fish digestive proteolytic enzymes from cold-adapted ectothermic organisms have found applications in several industries because their temperature requirements and other characteristics differ from those of homologous proteases from warm-blooded animals. Herein, we describe detection, partial purification, and characterization of proteolytic enzymes from the viscera and heads of Engraulis anchoita. Enzymatic activities of the by-products were assayed using azocaseín as a substrate. To characterize the alkali protein fraction, the activity against inhibitors and their molecular weights were studied. The crude protein extract exhibited maximal activity at pH 8.0 and 60EC. Results relative to the substrate-specific Nabenzoyl-DL-arginine-p-nitroanilide and the 25 kDa molecular weight indicated that the recovered protease was trypsin. The activity showed an increment in presence of SDS and a slight decrease when it was incubated with EDTA. Increasing the concentration of NaCl up to 5% did not significantly decrease the protein’s activity. The results obtained suggest that by-products of anchovy industry could be used in the detergents industry.Fil: Lamas, Daniela Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Subsede Instituto Nacional de Investigación y Desarrollo Pesquero; ArgentinaFil: Yeannes, Maria Isabel. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Massa, Agueda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Subsede Instituto Nacional de Investigación y Desarrollo Pesquero; ArgentinaInstitute of Bioorganic Chemistry2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78152Lamas, Daniela Lorena; Yeannes, Maria Isabel; Massa, Agueda Elena; Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization; Institute of Bioorganic Chemistry; Biotechnologia; 98; 2; 7-2017; 103-1120860-7796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5114/bta.2017.68309info:eu-repo/semantics/altIdentifier/url/http://www.biotechnologia-journal.org/Alkaline-trypsin-from-the-viscera-and-heads-of-Engraulis-anchoita-partial-purification-r-nand-characterization,85,30107,0,1.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:03Zoai:ri.conicet.gov.ar:11336/78152instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:03.456CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization
title Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization
spellingShingle Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization
Lamas, Daniela Lorena
ALKALINE PROTEASES
ENGRAULIS ANCHOITA
HEADS
TRYPSIN
VISCERA
title_short Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization
title_full Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization
title_fullStr Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization
title_full_unstemmed Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization
title_sort Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization
dc.creator.none.fl_str_mv Lamas, Daniela Lorena
Yeannes, Maria Isabel
Massa, Agueda Elena
author Lamas, Daniela Lorena
author_facet Lamas, Daniela Lorena
Yeannes, Maria Isabel
Massa, Agueda Elena
author_role author
author2 Yeannes, Maria Isabel
Massa, Agueda Elena
author2_role author
author
dc.subject.none.fl_str_mv ALKALINE PROTEASES
ENGRAULIS ANCHOITA
HEADS
TRYPSIN
VISCERA
topic ALKALINE PROTEASES
ENGRAULIS ANCHOITA
HEADS
TRYPSIN
VISCERA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Marine by-products contain valuable protein fractions. To ensure a profitable utilization of wastes from the fish industry, the knowledge about their quality and composition is necessary. Fish digestive proteolytic enzymes from cold-adapted ectothermic organisms have found applications in several industries because their temperature requirements and other characteristics differ from those of homologous proteases from warm-blooded animals. Herein, we describe detection, partial purification, and characterization of proteolytic enzymes from the viscera and heads of Engraulis anchoita. Enzymatic activities of the by-products were assayed using azocaseín as a substrate. To characterize the alkali protein fraction, the activity against inhibitors and their molecular weights were studied. The crude protein extract exhibited maximal activity at pH 8.0 and 60EC. Results relative to the substrate-specific Nabenzoyl-DL-arginine-p-nitroanilide and the 25 kDa molecular weight indicated that the recovered protease was trypsin. The activity showed an increment in presence of SDS and a slight decrease when it was incubated with EDTA. Increasing the concentration of NaCl up to 5% did not significantly decrease the protein’s activity. The results obtained suggest that by-products of anchovy industry could be used in the detergents industry.
Fil: Lamas, Daniela Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Subsede Instituto Nacional de Investigación y Desarrollo Pesquero; Argentina
Fil: Yeannes, Maria Isabel. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Massa, Agueda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Subsede Instituto Nacional de Investigación y Desarrollo Pesquero; Argentina
description Marine by-products contain valuable protein fractions. To ensure a profitable utilization of wastes from the fish industry, the knowledge about their quality and composition is necessary. Fish digestive proteolytic enzymes from cold-adapted ectothermic organisms have found applications in several industries because their temperature requirements and other characteristics differ from those of homologous proteases from warm-blooded animals. Herein, we describe detection, partial purification, and characterization of proteolytic enzymes from the viscera and heads of Engraulis anchoita. Enzymatic activities of the by-products were assayed using azocaseín as a substrate. To characterize the alkali protein fraction, the activity against inhibitors and their molecular weights were studied. The crude protein extract exhibited maximal activity at pH 8.0 and 60EC. Results relative to the substrate-specific Nabenzoyl-DL-arginine-p-nitroanilide and the 25 kDa molecular weight indicated that the recovered protease was trypsin. The activity showed an increment in presence of SDS and a slight decrease when it was incubated with EDTA. Increasing the concentration of NaCl up to 5% did not significantly decrease the protein’s activity. The results obtained suggest that by-products of anchovy industry could be used in the detergents industry.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78152
Lamas, Daniela Lorena; Yeannes, Maria Isabel; Massa, Agueda Elena; Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization; Institute of Bioorganic Chemistry; Biotechnologia; 98; 2; 7-2017; 103-112
0860-7796
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78152
identifier_str_mv Lamas, Daniela Lorena; Yeannes, Maria Isabel; Massa, Agueda Elena; Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization; Institute of Bioorganic Chemistry; Biotechnologia; 98; 2; 7-2017; 103-112
0860-7796
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5114/bta.2017.68309
info:eu-repo/semantics/altIdentifier/url/http://www.biotechnologia-journal.org/Alkaline-trypsin-from-the-viscera-and-heads-of-Engraulis-anchoita-partial-purification-r-nand-characterization,85,30107,0,1.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Institute of Bioorganic Chemistry
publisher.none.fl_str_mv Institute of Bioorganic Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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