Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5

Autores
Nieves Cordones, Manuel; Lara, Alberto; Silva, Martha; Amo, Jesús; Rodriguez Sepulveda, Pascual; Rivero, Rosa M.; Martínez, Vicente; Botella, María Ángeles; Rubio, Francisco
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Root K+ acquisition is a key process for plant growth and development, extensively studied in the model plant Arabidopsis thaliana. Because important differences may exist among species, translational research supported by specific studies is needed in crops such as tomato. Here we present a reverse genetics study to demonstrate the role of the SlHAK5 K+ transporter in tomato K+ nutrition, Cs+ accumulation and its fertility. slhak5 KO lines, generated by CRISPR-Cas edition, were characterized in growth experiments, Rb+ and Cs+ uptake tests and root cells K+-induced plasma membrane depolarizations. Pollen viability and its K+ accumulation capacity were estimated by using the K+-sensitive dye Ion Potassium Green 4. SlHAK5 is the major system for high-affinity root K+ uptake required for plant growth at low K+, even in the presence of salinity. It also constitutes a pathway for Cs+ entry in tomato plants with a strong impact on fruit Cs+ accumulation. SlHAK5 also contributes to pollen K+ uptake and viability and its absence produces almost seedless fruits. Knowledge gained into SlHAK5 can serve as a model for other crops with fleshy fruits and it can help to generate tools to develop low Cs+ or seedless fruits crops.
Fil: Nieves Cordones, Manuel. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Lara, Alberto. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Silva, Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Amo, Jesús. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Rodriguez Sepulveda, Pascual. No especifíca;
Fil: Rivero, Rosa M.. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Martínez, Vicente. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Botella, María Ángeles. Universidad de Miguel Hernández; España
Fil: Rubio, Francisco. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Materia
CAESIUM
POLLEN
POTASSIUM
SALINITY
TRANSPORT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/169480
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/169480
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5Nieves Cordones, ManuelLara, AlbertoSilva, MarthaAmo, JesúsRodriguez Sepulveda, PascualRivero, Rosa M.Martínez, VicenteBotella, María ÁngelesRubio, FranciscoCAESIUMPOLLENPOTASSIUMSALINITYTRANSPORThttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Root K+ acquisition is a key process for plant growth and development, extensively studied in the model plant Arabidopsis thaliana. Because important differences may exist among species, translational research supported by specific studies is needed in crops such as tomato. Here we present a reverse genetics study to demonstrate the role of the SlHAK5 K+ transporter in tomato K+ nutrition, Cs+ accumulation and its fertility. slhak5 KO lines, generated by CRISPR-Cas edition, were characterized in growth experiments, Rb+ and Cs+ uptake tests and root cells K+-induced plasma membrane depolarizations. Pollen viability and its K+ accumulation capacity were estimated by using the K+-sensitive dye Ion Potassium Green 4. SlHAK5 is the major system for high-affinity root K+ uptake required for plant growth at low K+, even in the presence of salinity. It also constitutes a pathway for Cs+ entry in tomato plants with a strong impact on fruit Cs+ accumulation. SlHAK5 also contributes to pollen K+ uptake and viability and its absence produces almost seedless fruits. Knowledge gained into SlHAK5 can serve as a model for other crops with fleshy fruits and it can help to generate tools to develop low Cs+ or seedless fruits crops.Fil: Nieves Cordones, Manuel. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Lara, Alberto. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Silva, Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Amo, Jesús. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Rodriguez Sepulveda, Pascual. No especifíca;Fil: Rivero, Rosa M.. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Martínez, Vicente. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Botella, María Ángeles. Universidad de Miguel Hernández; EspañaFil: Rubio, Francisco. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaWiley Blackwell Publishing, Inc2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/169480Nieves Cordones, Manuel; Lara, Alberto; Silva, Martha; Amo, Jesús; Rodriguez Sepulveda, Pascual; et al.; Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5; Wiley Blackwell Publishing, Inc; Plant, Cell and Environment; 43; 7; 7-2020; 1707-17210140-7791CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/pce.13769info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/pce.13769info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:26:20Zoai:ri.conicet.gov.ar:11336/169480instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:26:20.654CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5
title Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5
spellingShingle Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5
Nieves Cordones, Manuel
CAESIUM
POLLEN
POTASSIUM
SALINITY
TRANSPORT
title_short Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5
title_full Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5
title_fullStr Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5
title_full_unstemmed Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5
title_sort Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5
dc.creator.none.fl_str_mv Nieves Cordones, Manuel
Lara, Alberto
Silva, Martha
Amo, Jesús
Rodriguez Sepulveda, Pascual
Rivero, Rosa M.
Martínez, Vicente
Botella, María Ángeles
Rubio, Francisco
author Nieves Cordones, Manuel
author_facet Nieves Cordones, Manuel
Lara, Alberto
Silva, Martha
Amo, Jesús
Rodriguez Sepulveda, Pascual
Rivero, Rosa M.
Martínez, Vicente
Botella, María Ángeles
Rubio, Francisco
author_role author
author2 Lara, Alberto
Silva, Martha
Amo, Jesús
Rodriguez Sepulveda, Pascual
Rivero, Rosa M.
Martínez, Vicente
Botella, María Ángeles
Rubio, Francisco
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CAESIUM
POLLEN
POTASSIUM
SALINITY
TRANSPORT
topic CAESIUM
POLLEN
POTASSIUM
SALINITY
TRANSPORT
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Root K+ acquisition is a key process for plant growth and development, extensively studied in the model plant Arabidopsis thaliana. Because important differences may exist among species, translational research supported by specific studies is needed in crops such as tomato. Here we present a reverse genetics study to demonstrate the role of the SlHAK5 K+ transporter in tomato K+ nutrition, Cs+ accumulation and its fertility. slhak5 KO lines, generated by CRISPR-Cas edition, were characterized in growth experiments, Rb+ and Cs+ uptake tests and root cells K+-induced plasma membrane depolarizations. Pollen viability and its K+ accumulation capacity were estimated by using the K+-sensitive dye Ion Potassium Green 4. SlHAK5 is the major system for high-affinity root K+ uptake required for plant growth at low K+, even in the presence of salinity. It also constitutes a pathway for Cs+ entry in tomato plants with a strong impact on fruit Cs+ accumulation. SlHAK5 also contributes to pollen K+ uptake and viability and its absence produces almost seedless fruits. Knowledge gained into SlHAK5 can serve as a model for other crops with fleshy fruits and it can help to generate tools to develop low Cs+ or seedless fruits crops.
Fil: Nieves Cordones, Manuel. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Lara, Alberto. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Silva, Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Amo, Jesús. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Rodriguez Sepulveda, Pascual. No especifíca;
Fil: Rivero, Rosa M.. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Martínez, Vicente. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Botella, María Ángeles. Universidad de Miguel Hernández; España
Fil: Rubio, Francisco. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
description Root K+ acquisition is a key process for plant growth and development, extensively studied in the model plant Arabidopsis thaliana. Because important differences may exist among species, translational research supported by specific studies is needed in crops such as tomato. Here we present a reverse genetics study to demonstrate the role of the SlHAK5 K+ transporter in tomato K+ nutrition, Cs+ accumulation and its fertility. slhak5 KO lines, generated by CRISPR-Cas edition, were characterized in growth experiments, Rb+ and Cs+ uptake tests and root cells K+-induced plasma membrane depolarizations. Pollen viability and its K+ accumulation capacity were estimated by using the K+-sensitive dye Ion Potassium Green 4. SlHAK5 is the major system for high-affinity root K+ uptake required for plant growth at low K+, even in the presence of salinity. It also constitutes a pathway for Cs+ entry in tomato plants with a strong impact on fruit Cs+ accumulation. SlHAK5 also contributes to pollen K+ uptake and viability and its absence produces almost seedless fruits. Knowledge gained into SlHAK5 can serve as a model for other crops with fleshy fruits and it can help to generate tools to develop low Cs+ or seedless fruits crops.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/169480
Nieves Cordones, Manuel; Lara, Alberto; Silva, Martha; Amo, Jesús; Rodriguez Sepulveda, Pascual; et al.; Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5; Wiley Blackwell Publishing, Inc; Plant, Cell and Environment; 43; 7; 7-2020; 1707-1721
0140-7791
CONICET Digital
CONICET
url http://hdl.handle.net/11336/169480
identifier_str_mv Nieves Cordones, Manuel; Lara, Alberto; Silva, Martha; Amo, Jesús; Rodriguez Sepulveda, Pascual; et al.; Root high-affinity K+ and Cs+ uptake and plant fertility in tomato plants are dependent on the activity of the high-affinity K+ transporter SlHAK5; Wiley Blackwell Publishing, Inc; Plant, Cell and Environment; 43; 7; 7-2020; 1707-1721
0140-7791
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/pce.13769
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/pce.13769
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.084122

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