Comparison of differents methods of sperm selection of llama raw semen

Autores
Santa Cruz, R.; Giuliano, S. M.; Gambarotta, M. C.; Morrell, J. M.; Abraham, M. C.; Miragaya, Marcelo; Carretero, Maria Ignacia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to compare the efficiency of different sperm selection methods applied to the same llama ejaculate. Four treatments were compared: two variants of the swim up technique (with and without seminal plasma), and two different colloids, Androcoll-E-Large and Percoll®. Using electroejaculation, 21 semen samples were obtained from 7 llama males (n = 7, r = 3). The ejaculates were incubated in a solution of 0.1% collagenase, to decrease thread formation, and then split into 4 aliquots: one aliquot was layered over a column of Androcoll-E-Large (SLC) and the second over a column of Percoll (45%). The third aliquot was deposited in a tube with culture medium and was incubated at a 45° angle for 30 min at 37 °C (SU1). The last aliquot was centrifuged to separate the spermatozoa and seminal plasma. The sperm pellet obtained was resuspended, and transferred to a tube with culture medium which was incubated at an angle of 45° for 30 min at 37 °C (SU2). Both aliquots SLC and P showed higher proportions of progressive motility and plasma membrane functionality (p ≤ 0.05) than raw semen. There were no significant differences (p > 0.05) in sperm viability and in normal spermatozoa between raw semen and treatments. Nevertheless, only SLC did not have a significant increase of bent tails. In conclusion SLC centrifugation would be the method of choice for selecting llama spermatozoa.
Fil: Santa Cruz, R.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Giuliano, S. M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Gambarotta, M. C.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Morrell, J. M.. Swedish University Of Agricultural Sciences. Faculty Of Veterinary Medicine And Animal Science; Suecia
Fil: Abraham, M. C.. Swedish University Of Agricultural Sciences. Faculty Of Veterinary Medicine And Animal Science; Suecia
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Carretero, Maria Ignacia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ANDROCOLL
LLAMA
PERCOLL
SPERM SELECTION
SWIM UP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/117653
Acceder
id CONICETDig_7b72bf271ca0de00ebde84142550c7f2
oai_identifier_str oai:ri.conicet.gov.ar:11336/117653
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of differents methods of sperm selection of llama raw semenSanta Cruz, R.Giuliano, S. M.Gambarotta, M. C.Morrell, J. M.Abraham, M. C.Miragaya, MarceloCarretero, Maria IgnaciaANDROCOLLLLAMAPERCOLLSPERM SELECTIONSWIM UPhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The objective of this study was to compare the efficiency of different sperm selection methods applied to the same llama ejaculate. Four treatments were compared: two variants of the swim up technique (with and without seminal plasma), and two different colloids, Androcoll-E-Large and Percoll®. Using electroejaculation, 21 semen samples were obtained from 7 llama males (n = 7, r = 3). The ejaculates were incubated in a solution of 0.1% collagenase, to decrease thread formation, and then split into 4 aliquots: one aliquot was layered over a column of Androcoll-E-Large (SLC) and the second over a column of Percoll (45%). The third aliquot was deposited in a tube with culture medium and was incubated at a 45° angle for 30 min at 37 °C (SU1). The last aliquot was centrifuged to separate the spermatozoa and seminal plasma. The sperm pellet obtained was resuspended, and transferred to a tube with culture medium which was incubated at an angle of 45° for 30 min at 37 °C (SU2). Both aliquots SLC and P showed higher proportions of progressive motility and plasma membrane functionality (p ≤ 0.05) than raw semen. There were no significant differences (p > 0.05) in sperm viability and in normal spermatozoa between raw semen and treatments. Nevertheless, only SLC did not have a significant increase of bent tails. In conclusion SLC centrifugation would be the method of choice for selecting llama spermatozoa.Fil: Santa Cruz, R.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Giuliano, S. M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Gambarotta, M. C.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Morrell, J. M.. Swedish University Of Agricultural Sciences. Faculty Of Veterinary Medicine And Animal Science; SueciaFil: Abraham, M. C.. Swedish University Of Agricultural Sciences. Faculty Of Veterinary Medicine And Animal Science; SueciaFil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Carretero, Maria Ignacia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117653Santa Cruz, R.; Giuliano, S. M.; Gambarotta, M. C.; Morrell, J. M.; Abraham, M. C.; et al.; Comparison of differents methods of sperm selection of llama raw semen; Elsevier Science; Animal Reproduction Science; 173; 10-2016; 8-120378-4320CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2016.08.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378432016303037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:30Zoai:ri.conicet.gov.ar:11336/117653instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:30.781CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of differents methods of sperm selection of llama raw semen
title Comparison of differents methods of sperm selection of llama raw semen
spellingShingle Comparison of differents methods of sperm selection of llama raw semen
Santa Cruz, R.
ANDROCOLL
LLAMA
PERCOLL
SPERM SELECTION
SWIM UP
title_short Comparison of differents methods of sperm selection of llama raw semen
title_full Comparison of differents methods of sperm selection of llama raw semen
title_fullStr Comparison of differents methods of sperm selection of llama raw semen
title_full_unstemmed Comparison of differents methods of sperm selection of llama raw semen
title_sort Comparison of differents methods of sperm selection of llama raw semen
dc.creator.none.fl_str_mv Santa Cruz, R.
Giuliano, S. M.
Gambarotta, M. C.
Morrell, J. M.
Abraham, M. C.
Miragaya, Marcelo
Carretero, Maria Ignacia
author Santa Cruz, R.
author_facet Santa Cruz, R.
Giuliano, S. M.
Gambarotta, M. C.
Morrell, J. M.
Abraham, M. C.
Miragaya, Marcelo
Carretero, Maria Ignacia
author_role author
author2 Giuliano, S. M.
Gambarotta, M. C.
Morrell, J. M.
Abraham, M. C.
Miragaya, Marcelo
Carretero, Maria Ignacia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANDROCOLL
LLAMA
PERCOLL
SPERM SELECTION
SWIM UP
topic ANDROCOLL
LLAMA
PERCOLL
SPERM SELECTION
SWIM UP
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objective of this study was to compare the efficiency of different sperm selection methods applied to the same llama ejaculate. Four treatments were compared: two variants of the swim up technique (with and without seminal plasma), and two different colloids, Androcoll-E-Large and Percoll®. Using electroejaculation, 21 semen samples were obtained from 7 llama males (n = 7, r = 3). The ejaculates were incubated in a solution of 0.1% collagenase, to decrease thread formation, and then split into 4 aliquots: one aliquot was layered over a column of Androcoll-E-Large (SLC) and the second over a column of Percoll (45%). The third aliquot was deposited in a tube with culture medium and was incubated at a 45° angle for 30 min at 37 °C (SU1). The last aliquot was centrifuged to separate the spermatozoa and seminal plasma. The sperm pellet obtained was resuspended, and transferred to a tube with culture medium which was incubated at an angle of 45° for 30 min at 37 °C (SU2). Both aliquots SLC and P showed higher proportions of progressive motility and plasma membrane functionality (p ≤ 0.05) than raw semen. There were no significant differences (p > 0.05) in sperm viability and in normal spermatozoa between raw semen and treatments. Nevertheless, only SLC did not have a significant increase of bent tails. In conclusion SLC centrifugation would be the method of choice for selecting llama spermatozoa.
Fil: Santa Cruz, R.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Giuliano, S. M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Gambarotta, M. C.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Morrell, J. M.. Swedish University Of Agricultural Sciences. Faculty Of Veterinary Medicine And Animal Science; Suecia
Fil: Abraham, M. C.. Swedish University Of Agricultural Sciences. Faculty Of Veterinary Medicine And Animal Science; Suecia
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Carretero, Maria Ignacia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The objective of this study was to compare the efficiency of different sperm selection methods applied to the same llama ejaculate. Four treatments were compared: two variants of the swim up technique (with and without seminal plasma), and two different colloids, Androcoll-E-Large and Percoll®. Using electroejaculation, 21 semen samples were obtained from 7 llama males (n = 7, r = 3). The ejaculates were incubated in a solution of 0.1% collagenase, to decrease thread formation, and then split into 4 aliquots: one aliquot was layered over a column of Androcoll-E-Large (SLC) and the second over a column of Percoll (45%). The third aliquot was deposited in a tube with culture medium and was incubated at a 45° angle for 30 min at 37 °C (SU1). The last aliquot was centrifuged to separate the spermatozoa and seminal plasma. The sperm pellet obtained was resuspended, and transferred to a tube with culture medium which was incubated at an angle of 45° for 30 min at 37 °C (SU2). Both aliquots SLC and P showed higher proportions of progressive motility and plasma membrane functionality (p ≤ 0.05) than raw semen. There were no significant differences (p > 0.05) in sperm viability and in normal spermatozoa between raw semen and treatments. Nevertheless, only SLC did not have a significant increase of bent tails. In conclusion SLC centrifugation would be the method of choice for selecting llama spermatozoa.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117653
Santa Cruz, R.; Giuliano, S. M.; Gambarotta, M. C.; Morrell, J. M.; Abraham, M. C.; et al.; Comparison of differents methods of sperm selection of llama raw semen; Elsevier Science; Animal Reproduction Science; 173; 10-2016; 8-12
0378-4320
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117653
identifier_str_mv Santa Cruz, R.; Giuliano, S. M.; Gambarotta, M. C.; Morrell, J. M.; Abraham, M. C.; et al.; Comparison of differents methods of sperm selection of llama raw semen; Elsevier Science; Animal Reproduction Science; 173; 10-2016; 8-12
0378-4320
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2016.08.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378432016303037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268976759963648
score 13.13397

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