Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery

Autores
Garbarino Azúa, Darío Jose; Saucedo, Lucia; Giordana, S.; Magri, M.L.; Buffone, Mariano Gabriel; Neuspiller, F.; Vazquez, Monica Hebe; Marin Briggiler, Clara Isabel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fibroblast growth factors (FGFs) and their receptors (FGFRs) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 (FGF2) in human spermatozoa, to determine the relationship of FGF2 levels with conventional semen parameters and to assess the effect of recombinant FGF2 (rFGF2) on sperm recovery in a selection procedure. Western immunoblotting analysis using an antibody against FGF2 revealed an 18-kDa band in sperm protein extracts. The protein was immunolocalized in the sperm flagellum and acrosomal region, as well as in all germ cells. Sperm FGF2 levels, assessed by flow cytometry, showed a positive (p < 0.05) correlation with sperm concentration, motility, total sperm number and total motile cells per ejaculate. Moreover, samples with abnormal motility depicted diminished (p < 0.01) FGF2 levels compared to those with normal motility. Spermatozoa exposed to rFGF2 bound the protein, exhibited higher (p < 0.05) total and motile sperm recoveries, and increased (p < 0.01) kinematic parameters after the swim-up. Findings herein presented lead to consider sperm FGF2 level as a potential marker of sperm quality, and rFGF2 as a supplement for improving sperm recovery in selection techniques.
Fil: Garbarino Azúa, Darío Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Giordana, S.. Instituto Valenciano de Infertilidad ; Argentina
Fil: Magri, M.L.. Instituto Valenciano de Infertilidad ; Argentina
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Neuspiller, F.. Instituto Valenciano de Infertilidad ; Argentina
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
Fgf2
Fibroblast Growth Factor 2
Motility
Spermatozoa
Swim-Up
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36420
Acceder
id CONICETDig_63891247ea1df10e0f96eb6b2087b7cb
oai_identifier_str oai:ri.conicet.gov.ar:11336/36420
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recoveryGarbarino Azúa, Darío JoseSaucedo, LuciaGiordana, S.Magri, M.L.Buffone, Mariano GabrielNeuspiller, F.Vazquez, Monica HebeMarin Briggiler, Clara IsabelFgf2Fibroblast Growth Factor 2MotilitySpermatozoaSwim-Uphttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fibroblast growth factors (FGFs) and their receptors (FGFRs) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 (FGF2) in human spermatozoa, to determine the relationship of FGF2 levels with conventional semen parameters and to assess the effect of recombinant FGF2 (rFGF2) on sperm recovery in a selection procedure. Western immunoblotting analysis using an antibody against FGF2 revealed an 18-kDa band in sperm protein extracts. The protein was immunolocalized in the sperm flagellum and acrosomal region, as well as in all germ cells. Sperm FGF2 levels, assessed by flow cytometry, showed a positive (p < 0.05) correlation with sperm concentration, motility, total sperm number and total motile cells per ejaculate. Moreover, samples with abnormal motility depicted diminished (p < 0.01) FGF2 levels compared to those with normal motility. Spermatozoa exposed to rFGF2 bound the protein, exhibited higher (p < 0.05) total and motile sperm recoveries, and increased (p < 0.01) kinematic parameters after the swim-up. Findings herein presented lead to consider sperm FGF2 level as a potential marker of sperm quality, and rFGF2 as a supplement for improving sperm recovery in selection techniques.Fil: Garbarino Azúa, Darío Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Giordana, S.. Instituto Valenciano de Infertilidad ; ArgentinaFil: Magri, M.L.. Instituto Valenciano de Infertilidad ; ArgentinaFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Neuspiller, F.. Instituto Valenciano de Infertilidad ; ArgentinaFil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaWiley-liss, Div John Wiley & Sons Inc2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36420Garbarino Azúa, Darío Jose; Saucedo, Lucia; Giordana, S.; Magri, M.L.; Buffone, Mariano Gabriel; et al.; Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery; Wiley-liss, Div John Wiley & Sons Inc; Andrology; 5; 5; 9-2017; 990-9982047-29192047-2927CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/andr.12398/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/andr.1239info:eu-repo/semantics/altIdentifier/pmid/28732140info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:27Zoai:ri.conicet.gov.ar:11336/36420instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:28.013CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery
title Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery
spellingShingle Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery
Garbarino Azúa, Darío Jose
Fgf2
Fibroblast Growth Factor 2
Motility
Spermatozoa
Swim-Up
title_short Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery
title_full Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery
title_fullStr Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery
title_full_unstemmed Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery
title_sort Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery
dc.creator.none.fl_str_mv Garbarino Azúa, Darío Jose
Saucedo, Lucia
Giordana, S.
Magri, M.L.
Buffone, Mariano Gabriel
Neuspiller, F.
Vazquez, Monica Hebe
Marin Briggiler, Clara Isabel
author Garbarino Azúa, Darío Jose
author_facet Garbarino Azúa, Darío Jose
Saucedo, Lucia
Giordana, S.
Magri, M.L.
Buffone, Mariano Gabriel
Neuspiller, F.
Vazquez, Monica Hebe
Marin Briggiler, Clara Isabel
author_role author
author2 Saucedo, Lucia
Giordana, S.
Magri, M.L.
Buffone, Mariano Gabriel
Neuspiller, F.
Vazquez, Monica Hebe
Marin Briggiler, Clara Isabel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fgf2
Fibroblast Growth Factor 2
Motility
Spermatozoa
Swim-Up
topic Fgf2
Fibroblast Growth Factor 2
Motility
Spermatozoa
Swim-Up
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fibroblast growth factors (FGFs) and their receptors (FGFRs) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 (FGF2) in human spermatozoa, to determine the relationship of FGF2 levels with conventional semen parameters and to assess the effect of recombinant FGF2 (rFGF2) on sperm recovery in a selection procedure. Western immunoblotting analysis using an antibody against FGF2 revealed an 18-kDa band in sperm protein extracts. The protein was immunolocalized in the sperm flagellum and acrosomal region, as well as in all germ cells. Sperm FGF2 levels, assessed by flow cytometry, showed a positive (p < 0.05) correlation with sperm concentration, motility, total sperm number and total motile cells per ejaculate. Moreover, samples with abnormal motility depicted diminished (p < 0.01) FGF2 levels compared to those with normal motility. Spermatozoa exposed to rFGF2 bound the protein, exhibited higher (p < 0.05) total and motile sperm recoveries, and increased (p < 0.01) kinematic parameters after the swim-up. Findings herein presented lead to consider sperm FGF2 level as a potential marker of sperm quality, and rFGF2 as a supplement for improving sperm recovery in selection techniques.
Fil: Garbarino Azúa, Darío Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Giordana, S.. Instituto Valenciano de Infertilidad ; Argentina
Fil: Magri, M.L.. Instituto Valenciano de Infertilidad ; Argentina
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Neuspiller, F.. Instituto Valenciano de Infertilidad ; Argentina
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Fibroblast growth factors (FGFs) and their receptors (FGFRs) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 (FGF2) in human spermatozoa, to determine the relationship of FGF2 levels with conventional semen parameters and to assess the effect of recombinant FGF2 (rFGF2) on sperm recovery in a selection procedure. Western immunoblotting analysis using an antibody against FGF2 revealed an 18-kDa band in sperm protein extracts. The protein was immunolocalized in the sperm flagellum and acrosomal region, as well as in all germ cells. Sperm FGF2 levels, assessed by flow cytometry, showed a positive (p < 0.05) correlation with sperm concentration, motility, total sperm number and total motile cells per ejaculate. Moreover, samples with abnormal motility depicted diminished (p < 0.01) FGF2 levels compared to those with normal motility. Spermatozoa exposed to rFGF2 bound the protein, exhibited higher (p < 0.05) total and motile sperm recoveries, and increased (p < 0.01) kinematic parameters after the swim-up. Findings herein presented lead to consider sperm FGF2 level as a potential marker of sperm quality, and rFGF2 as a supplement for improving sperm recovery in selection techniques.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36420
Garbarino Azúa, Darío Jose; Saucedo, Lucia; Giordana, S.; Magri, M.L.; Buffone, Mariano Gabriel; et al.; Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery; Wiley-liss, Div John Wiley & Sons Inc; Andrology; 5; 5; 9-2017; 990-998
2047-2919
2047-2927
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36420
identifier_str_mv Garbarino Azúa, Darío Jose; Saucedo, Lucia; Giordana, S.; Magri, M.L.; Buffone, Mariano Gabriel; et al.; Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery; Wiley-liss, Div John Wiley & Sons Inc; Andrology; 5; 5; 9-2017; 990-998
2047-2919
2047-2927
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/andr.12398/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/andr.1239
info:eu-repo/semantics/altIdentifier/pmid/28732140
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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