Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation

Autores
Buffone, Mariano Gabriel; Doncel, G. F.; Marin Briggiler, Clara Isabel; Vazquez, Monica Hebe; Calamera, J. C.
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human semen is composed of a heterogeneous population of sperm with varying degrees of structural and functional differentiation and normality, which result in subpopulations of different quality. Using a discontinuous Percoll gradient, we separated three subsets of sperm [(45%; L45), (65%; L65) and (90%; L90) fractions] from normozoospermic human semen samples from healthy donors and proceeded to characterize their morphology, motility and hyperactivation, as well as their ability to undergo tyrosine phosphorylation under capacitating conditions.
Fil: Buffone, Mariano Gabriel. Laboratorio de Estudios en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Doncel, G. F.. Eastern Virginia Medical School; Estados Unidos
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Calamera, J. C.. Laboratorio de Estudios en Reproducción; Argentina
Materia
Capacitation
Percoll Gradient
Protein Tyrosine Phosphorylation
Human Sperm
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29108
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylationBuffone, Mariano GabrielDoncel, G. F.Marin Briggiler, Clara IsabelVazquez, Monica HebeCalamera, J. C.CapacitationPercoll GradientProtein Tyrosine PhosphorylationHuman Spermhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Human semen is composed of a heterogeneous population of sperm with varying degrees of structural and functional differentiation and normality, which result in subpopulations of different quality. Using a discontinuous Percoll gradient, we separated three subsets of sperm [(45%; L45), (65%; L65) and (90%; L90) fractions] from normozoospermic human semen samples from healthy donors and proceeded to characterize their morphology, motility and hyperactivation, as well as their ability to undergo tyrosine phosphorylation under capacitating conditions.Fil: Buffone, Mariano Gabriel. Laboratorio de Estudios en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Doncel, G. F.. Eastern Virginia Medical School; Estados UnidosFil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Calamera, J. C.. Laboratorio de Estudios en Reproducción; ArgentinaEuropean Society of Human Reproduction and Embriology2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29108Buffone, Mariano Gabriel; Doncel, G. F.; Marin Briggiler, Clara Isabel; Vazquez, Monica Hebe; Calamera, J. C.; Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation; European Society of Human Reproduction and Embriology; Human Reproduction; 19; 1; 12-2004; 139-1460268-11611460-2350CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/humrep/article/19/1/139/690201info:eu-repo/semantics/altIdentifier/doi/10.1093/humrep/deh040info:eu-repo/semantics/altIdentifier/pmid/14688172info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:32Zoai:ri.conicet.gov.ar:11336/29108instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:32.277CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation
title Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation
spellingShingle Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation
Buffone, Mariano Gabriel
Capacitation
Percoll Gradient
Protein Tyrosine Phosphorylation
Human Sperm
title_short Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation
title_full Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation
title_fullStr Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation
title_full_unstemmed Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation
title_sort Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation
dc.creator.none.fl_str_mv Buffone, Mariano Gabriel
Doncel, G. F.
Marin Briggiler, Clara Isabel
Vazquez, Monica Hebe
Calamera, J. C.
author Buffone, Mariano Gabriel
author_facet Buffone, Mariano Gabriel
Doncel, G. F.
Marin Briggiler, Clara Isabel
Vazquez, Monica Hebe
Calamera, J. C.
author_role author
author2 Doncel, G. F.
Marin Briggiler, Clara Isabel
Vazquez, Monica Hebe
Calamera, J. C.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Capacitation
Percoll Gradient
Protein Tyrosine Phosphorylation
Human Sperm
topic Capacitation
Percoll Gradient
Protein Tyrosine Phosphorylation
Human Sperm
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Human semen is composed of a heterogeneous population of sperm with varying degrees of structural and functional differentiation and normality, which result in subpopulations of different quality. Using a discontinuous Percoll gradient, we separated three subsets of sperm [(45%; L45), (65%; L65) and (90%; L90) fractions] from normozoospermic human semen samples from healthy donors and proceeded to characterize their morphology, motility and hyperactivation, as well as their ability to undergo tyrosine phosphorylation under capacitating conditions.
Fil: Buffone, Mariano Gabriel. Laboratorio de Estudios en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Doncel, G. F.. Eastern Virginia Medical School; Estados Unidos
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Calamera, J. C.. Laboratorio de Estudios en Reproducción; Argentina
description Human semen is composed of a heterogeneous population of sperm with varying degrees of structural and functional differentiation and normality, which result in subpopulations of different quality. Using a discontinuous Percoll gradient, we separated three subsets of sperm [(45%; L45), (65%; L65) and (90%; L90) fractions] from normozoospermic human semen samples from healthy donors and proceeded to characterize their morphology, motility and hyperactivation, as well as their ability to undergo tyrosine phosphorylation under capacitating conditions.
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29108
Buffone, Mariano Gabriel; Doncel, G. F.; Marin Briggiler, Clara Isabel; Vazquez, Monica Hebe; Calamera, J. C.; Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation; European Society of Human Reproduction and Embriology; Human Reproduction; 19; 1; 12-2004; 139-146
0268-1161
1460-2350
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29108
identifier_str_mv Buffone, Mariano Gabriel; Doncel, G. F.; Marin Briggiler, Clara Isabel; Vazquez, Monica Hebe; Calamera, J. C.; Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation; European Society of Human Reproduction and Embriology; Human Reproduction; 19; 1; 12-2004; 139-146
0268-1161
1460-2350
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/humrep/article/19/1/139/690201
info:eu-repo/semantics/altIdentifier/doi/10.1093/humrep/deh040
info:eu-repo/semantics/altIdentifier/pmid/14688172
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv European Society of Human Reproduction and Embriology
publisher.none.fl_str_mv European Society of Human Reproduction and Embriology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269165147127808
score 13.13397

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