Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)

Autores
Gómez Lobato, María Eugenia; Hasperué, Héctor Joaquín; Civello, Pedro Marcos; Chaves, Alicia Raquel; Martínez, Gustavo Adolfo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Degreening caused by chlorophyll degradation is the most evident visual manifestation of broccoli senescence and deterioration. In this work, the effect of 1-MCP on color change, chlorophyll degradation and expression of genes associated to chlorophyll catabolism was evaluated during postharvest storage. 1-MCP treatment markedly delayed degreening and reduction of chlorophyll content. The expression of genes encoding chlorophyllases showed different behaviors. In controls, the expression of BoCLH1 decreased during postharvest storage while that of BoCLH2 increased after 72 h and then decreased. The treatment with 1-MCP did not cause any effect on BoCLH1 but induced a higher expression of BoCLH2. A high BoPPH expression level was found in senescent broccoli florets, but the up-regulation of this gene was delayed by 1-MCP treatment. The expression of BoPaO, a gene encoding a pheophorbide a oxygenase, increased after three days and then decreased. In contrast, this expression was reduced in treated broccoli. Finally, the expression of BoRCCR decreased during senescence and the treatment with 1-MCP caused a higher decrement. These results show that postharvest 1-MCP inhibit selectively some of the genes encoding enzymes related chlorophyll catabolism.
Instituto de Fisiología Vegetal
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Ciencias Exactas
Ciencias Agrarias
broccoli
postharvest
senescence
1-MCP
chlorophyll catabolic enzyme
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/159965
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/159965
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)Gómez Lobato, María EugeniaHasperué, Héctor JoaquínCivello, Pedro MarcosChaves, Alicia RaquelMartínez, Gustavo AdolfoCiencias ExactasCiencias Agrariasbroccolipostharvestsenescence1-MCPchlorophyll catabolic enzymeDegreening caused by chlorophyll degradation is the most evident visual manifestation of broccoli senescence and deterioration. In this work, the effect of 1-MCP on color change, chlorophyll degradation and expression of genes associated to chlorophyll catabolism was evaluated during postharvest storage. 1-MCP treatment markedly delayed degreening and reduction of chlorophyll content. The expression of genes encoding chlorophyllases showed different behaviors. In controls, the expression of BoCLH1 decreased during postharvest storage while that of BoCLH2 increased after 72 h and then decreased. The treatment with 1-MCP did not cause any effect on BoCLH1 but induced a higher expression of BoCLH2. A high BoPPH expression level was found in senescent broccoli florets, but the up-regulation of this gene was delayed by 1-MCP treatment. The expression of BoPaO, a gene encoding a pheophorbide a oxygenase, increased after three days and then decreased. In contrast, this expression was reduced in treated broccoli. Finally, the expression of BoRCCR decreased during senescence and the treatment with 1-MCP caused a higher decrement. These results show that postharvest 1-MCP inhibit selectively some of the genes encoding enzymes related chlorophyll catabolism.Instituto de Fisiología VegetalCentro de Investigación y Desarrollo en Criotecnología de Alimentos2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf208-211http://sedici.unlp.edu.ar/handle/10915/159965enginfo:eu-repo/semantics/altIdentifier/issn/0304-4238info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2012.07.017info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:13:49Zoai:sedici.unlp.edu.ar:10915/159965Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:13:50.587SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
spellingShingle Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
Gómez Lobato, María Eugenia
Ciencias Exactas
Ciencias Agrarias
broccoli
postharvest
senescence
1-MCP
chlorophyll catabolic enzyme
title_short Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title_full Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title_fullStr Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title_full_unstemmed Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title_sort Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
dc.creator.none.fl_str_mv Gómez Lobato, María Eugenia
Hasperué, Héctor Joaquín
Civello, Pedro Marcos
Chaves, Alicia Raquel
Martínez, Gustavo Adolfo
author Gómez Lobato, María Eugenia
author_facet Gómez Lobato, María Eugenia
Hasperué, Héctor Joaquín
Civello, Pedro Marcos
Chaves, Alicia Raquel
Martínez, Gustavo Adolfo
author_role author
author2 Hasperué, Héctor Joaquín
Civello, Pedro Marcos
Chaves, Alicia Raquel
Martínez, Gustavo Adolfo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Ciencias Agrarias
broccoli
postharvest
senescence
1-MCP
chlorophyll catabolic enzyme
topic Ciencias Exactas
Ciencias Agrarias
broccoli
postharvest
senescence
1-MCP
chlorophyll catabolic enzyme
dc.description.none.fl_txt_mv Degreening caused by chlorophyll degradation is the most evident visual manifestation of broccoli senescence and deterioration. In this work, the effect of 1-MCP on color change, chlorophyll degradation and expression of genes associated to chlorophyll catabolism was evaluated during postharvest storage. 1-MCP treatment markedly delayed degreening and reduction of chlorophyll content. The expression of genes encoding chlorophyllases showed different behaviors. In controls, the expression of BoCLH1 decreased during postharvest storage while that of BoCLH2 increased after 72 h and then decreased. The treatment with 1-MCP did not cause any effect on BoCLH1 but induced a higher expression of BoCLH2. A high BoPPH expression level was found in senescent broccoli florets, but the up-regulation of this gene was delayed by 1-MCP treatment. The expression of BoPaO, a gene encoding a pheophorbide a oxygenase, increased after three days and then decreased. In contrast, this expression was reduced in treated broccoli. Finally, the expression of BoRCCR decreased during senescence and the treatment with 1-MCP caused a higher decrement. These results show that postharvest 1-MCP inhibit selectively some of the genes encoding enzymes related chlorophyll catabolism.
Instituto de Fisiología Vegetal
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description Degreening caused by chlorophyll degradation is the most evident visual manifestation of broccoli senescence and deterioration. In this work, the effect of 1-MCP on color change, chlorophyll degradation and expression of genes associated to chlorophyll catabolism was evaluated during postharvest storage. 1-MCP treatment markedly delayed degreening and reduction of chlorophyll content. The expression of genes encoding chlorophyllases showed different behaviors. In controls, the expression of BoCLH1 decreased during postharvest storage while that of BoCLH2 increased after 72 h and then decreased. The treatment with 1-MCP did not cause any effect on BoCLH1 but induced a higher expression of BoCLH2. A high BoPPH expression level was found in senescent broccoli florets, but the up-regulation of this gene was delayed by 1-MCP treatment. The expression of BoPaO, a gene encoding a pheophorbide a oxygenase, increased after three days and then decreased. In contrast, this expression was reduced in treated broccoli. Finally, the expression of BoRCCR decreased during senescence and the treatment with 1-MCP caused a higher decrement. These results show that postharvest 1-MCP inhibit selectively some of the genes encoding enzymes related chlorophyll catabolism.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/159965
url http://sedici.unlp.edu.ar/handle/10915/159965
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0304-4238
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2012.07.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
208-211
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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