Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain

Autores
Traglia, German Matias; Place, Kori; Dotto, Cristian Marcelo; Fernandez, Jennifer; Montaña, Sabrina Daiana; Bahiense, Camila dos Santos; Soler Bistue, Alfonso J. C.; Iriarte, Andres; Perez, Federico; Tolmasky, Marcelo E.; Bonomo, Robert A.; Melano, Roberto Gustavo; Ramirez, Maria Soledad
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The human pathogen Acinetobacter baumannii possesses high genetic plasticity and frequently acquires antimicrobial resistance genes. Here we investigated the role of natural transformation in these processes. Genomic DNA from different sources, including from carbapenem-resistant Klebsiella pneumoniae strains, was mixed with A. baumannii A118 cells. Selected transformants were analysed by whole-genome sequencing. In addition, bioinformatics analyses and in silico gene flow prediction were also performed to support the experimental results. Transformant strains included some that became resistant to carbapenems or changed their antimicrobial susceptibility profile. Foreign DNA acquisition was confirmed by whole-genome analysis. The acquired DNA most frequently identified corresponded to mobile genetic elements, antimicrobial resistance genes and operons involved in metabolism. Bioinformatics analyses and in silico gene flow prediction showed continued exchange of genetic material between A. baumannii and K. pneumoniae when they share the same habitat. Natural transformation plays an important role in the plasticity of A. baumannii and concomitantly in the emergence of multidrug-resistant strains.
Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Place, Kori. California State University; Estados Unidos
Fil: Dotto, Cristian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Fernandez, Jennifer. California State University; Estados Unidos
Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Bahiense, Camila dos Santos. California State University; Estados Unidos
Fil: Soler Bistue, Alfonso J. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Iriarte, Andres. Universidad de la Republica. Facultad de Medicina; Uruguay
Fil: Perez, Federico. Louis Stokes Cleveland Department Of Veterans Affairs; Estados Unidos
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Department Of Veterans Affairs; Estados Unidos
Fil: Melano, Roberto Gustavo. Public Health Ontario Laboratories; Canadá
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
Materia
Natural trasnformation
Acinetobacter baumannii
Genome plasticity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105320
Acceder
id CONICETDig_7b049a8a89368837cb431e980171090c
oai_identifier_str oai:ri.conicet.gov.ar:11336/105320
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strainTraglia, German MatiasPlace, KoriDotto, Cristian MarceloFernandez, JenniferMontaña, Sabrina DaianaBahiense, Camila dos SantosSoler Bistue, Alfonso J. C.Iriarte, AndresPerez, FedericoTolmasky, Marcelo E.Bonomo, Robert A.Melano, Roberto GustavoRamirez, Maria SoledadNatural trasnformationAcinetobacter baumanniiGenome plasticityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The human pathogen Acinetobacter baumannii possesses high genetic plasticity and frequently acquires antimicrobial resistance genes. Here we investigated the role of natural transformation in these processes. Genomic DNA from different sources, including from carbapenem-resistant Klebsiella pneumoniae strains, was mixed with A. baumannii A118 cells. Selected transformants were analysed by whole-genome sequencing. In addition, bioinformatics analyses and in silico gene flow prediction were also performed to support the experimental results. Transformant strains included some that became resistant to carbapenems or changed their antimicrobial susceptibility profile. Foreign DNA acquisition was confirmed by whole-genome analysis. The acquired DNA most frequently identified corresponded to mobile genetic elements, antimicrobial resistance genes and operons involved in metabolism. Bioinformatics analyses and in silico gene flow prediction showed continued exchange of genetic material between A. baumannii and K. pneumoniae when they share the same habitat. Natural transformation plays an important role in the plasticity of A. baumannii and concomitantly in the emergence of multidrug-resistant strains.Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Place, Kori. California State University; Estados UnidosFil: Dotto, Cristian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Fernandez, Jennifer. California State University; Estados UnidosFil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Bahiense, Camila dos Santos. California State University; Estados UnidosFil: Soler Bistue, Alfonso J. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Iriarte, Andres. Universidad de la Republica. Facultad de Medicina; UruguayFil: Perez, Federico. Louis Stokes Cleveland Department Of Veterans Affairs; Estados UnidosFil: Tolmasky, Marcelo E.. California State University; Estados UnidosFil: Bonomo, Robert A.. Louis Stokes Cleveland Department Of Veterans Affairs; Estados UnidosFil: Melano, Roberto Gustavo. Public Health Ontario Laboratories; CanadáFil: Ramirez, Maria Soledad. California State University; Estados UnidosElsevier Science2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105320Traglia, German Matias; Place, Kori; Dotto, Cristian Marcelo; Fernandez, Jennifer; Montaña, Sabrina Daiana; et al.; Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain; Elsevier Science; International Journal of Antimicrobial Agents; 53; 4; 4-2019; 483-4900924-85791872-7913CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijantimicag.2018.12.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0924857918303832?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:27Zoai:ri.conicet.gov.ar:11336/105320instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:27.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain
title Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain
spellingShingle Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain
Traglia, German Matias
Natural trasnformation
Acinetobacter baumannii
Genome plasticity
title_short Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain
title_full Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain
title_fullStr Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain
title_full_unstemmed Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain
title_sort Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain
dc.creator.none.fl_str_mv Traglia, German Matias
Place, Kori
Dotto, Cristian Marcelo
Fernandez, Jennifer
Montaña, Sabrina Daiana
Bahiense, Camila dos Santos
Soler Bistue, Alfonso J. C.
Iriarte, Andres
Perez, Federico
Tolmasky, Marcelo E.
Bonomo, Robert A.
Melano, Roberto Gustavo
Ramirez, Maria Soledad
author Traglia, German Matias
author_facet Traglia, German Matias
Place, Kori
Dotto, Cristian Marcelo
Fernandez, Jennifer
Montaña, Sabrina Daiana
Bahiense, Camila dos Santos
Soler Bistue, Alfonso J. C.
Iriarte, Andres
Perez, Federico
Tolmasky, Marcelo E.
Bonomo, Robert A.
Melano, Roberto Gustavo
Ramirez, Maria Soledad
author_role author
author2 Place, Kori
Dotto, Cristian Marcelo
Fernandez, Jennifer
Montaña, Sabrina Daiana
Bahiense, Camila dos Santos
Soler Bistue, Alfonso J. C.
Iriarte, Andres
Perez, Federico
Tolmasky, Marcelo E.
Bonomo, Robert A.
Melano, Roberto Gustavo
Ramirez, Maria Soledad
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Natural trasnformation
Acinetobacter baumannii
Genome plasticity
topic Natural trasnformation
Acinetobacter baumannii
Genome plasticity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The human pathogen Acinetobacter baumannii possesses high genetic plasticity and frequently acquires antimicrobial resistance genes. Here we investigated the role of natural transformation in these processes. Genomic DNA from different sources, including from carbapenem-resistant Klebsiella pneumoniae strains, was mixed with A. baumannii A118 cells. Selected transformants were analysed by whole-genome sequencing. In addition, bioinformatics analyses and in silico gene flow prediction were also performed to support the experimental results. Transformant strains included some that became resistant to carbapenems or changed their antimicrobial susceptibility profile. Foreign DNA acquisition was confirmed by whole-genome analysis. The acquired DNA most frequently identified corresponded to mobile genetic elements, antimicrobial resistance genes and operons involved in metabolism. Bioinformatics analyses and in silico gene flow prediction showed continued exchange of genetic material between A. baumannii and K. pneumoniae when they share the same habitat. Natural transformation plays an important role in the plasticity of A. baumannii and concomitantly in the emergence of multidrug-resistant strains.
Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Place, Kori. California State University; Estados Unidos
Fil: Dotto, Cristian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Fernandez, Jennifer. California State University; Estados Unidos
Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Bahiense, Camila dos Santos. California State University; Estados Unidos
Fil: Soler Bistue, Alfonso J. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Iriarte, Andres. Universidad de la Republica. Facultad de Medicina; Uruguay
Fil: Perez, Federico. Louis Stokes Cleveland Department Of Veterans Affairs; Estados Unidos
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Department Of Veterans Affairs; Estados Unidos
Fil: Melano, Roberto Gustavo. Public Health Ontario Laboratories; Canadá
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
description The human pathogen Acinetobacter baumannii possesses high genetic plasticity and frequently acquires antimicrobial resistance genes. Here we investigated the role of natural transformation in these processes. Genomic DNA from different sources, including from carbapenem-resistant Klebsiella pneumoniae strains, was mixed with A. baumannii A118 cells. Selected transformants were analysed by whole-genome sequencing. In addition, bioinformatics analyses and in silico gene flow prediction were also performed to support the experimental results. Transformant strains included some that became resistant to carbapenems or changed their antimicrobial susceptibility profile. Foreign DNA acquisition was confirmed by whole-genome analysis. The acquired DNA most frequently identified corresponded to mobile genetic elements, antimicrobial resistance genes and operons involved in metabolism. Bioinformatics analyses and in silico gene flow prediction showed continued exchange of genetic material between A. baumannii and K. pneumoniae when they share the same habitat. Natural transformation plays an important role in the plasticity of A. baumannii and concomitantly in the emergence of multidrug-resistant strains.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105320
Traglia, German Matias; Place, Kori; Dotto, Cristian Marcelo; Fernandez, Jennifer; Montaña, Sabrina Daiana; et al.; Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain; Elsevier Science; International Journal of Antimicrobial Agents; 53; 4; 4-2019; 483-490
0924-8579
1872-7913
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105320
identifier_str_mv Traglia, German Matias; Place, Kori; Dotto, Cristian Marcelo; Fernandez, Jennifer; Montaña, Sabrina Daiana; et al.; Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain; Elsevier Science; International Journal of Antimicrobial Agents; 53; 4; 4-2019; 483-490
0924-8579
1872-7913
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijantimicag.2018.12.013
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0924857918303832?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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