MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

• Autores: Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Korntved, Anne Kirstine C.; Rudkjøbing, Vibeke; Alves, Madalena; Bajón Fernández, Yadira; Batstone, Damien; Butler, Caitlyn; Cruz, Mercedes Cecilia; Davidsson, Åsa; Erijman, Leonardo; Holliger, Christof; Koch, Konrad; Kreuzinger, Norbert; Lee, Changsoo; Lyberatos, Gerasimos; Mutnuri, Srikanth; O´Flaherty, Vincent; Oleskowicz Popiel, Piotr; Pokorna, Dana; Rajal, Verónica Beatriz; Recktenwald, Michael; Rodríguez, Jorge; Saikaly, Pascal E.; Tooker, Nick; Vierheilig, Julia; De Vrieze, Jo; Wurzbacher, Christian; Nielsen, Per Halkjær
• Año de publicación: 2024
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: MICROBIOTA; ANAEROBIC DIGESTION; ORGANIC WASTE; BIOGAS
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and are used to expand the MiDAS 4 database for bacteria and archaea in wastewater treatment systems, creating MiDAS 5. The expansion of the MiDAS database increases the coverage for bacteria and archaea in ADs worldwide, leading to improved genus- and species-level classification. Using MiDAS 5, we carry out an amplicon-based, global-scale microbial community profiling of the sampled ADs using three common sets of primers targeting different regions of the 16S rRNA gene in bacteria and/or archaea. We reveal how environmental conditions and biogeography shape the AD microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 692 genera and 1013 species. These represent 84–99% and 18–61% of the accumulated read abundance, respectively, across samples depending on the amplicon primers used. Finally, we examine the global diversity of functional groups with known importance for the anaerobic digestion process.
Fil: Dueholm, Morten Kam Dahl. Aalborg University; Dinamarca
Fil: Andersen, Kasper Skytte. Aalborg University; Dinamarca
Fil: Korntved, Anne Kirstine C.. Aalborg University; Dinamarca
Fil: Rudkjøbing, Vibeke. Aalborg University; Dinamarca
Fil: Alves, Madalena. Universidade do Minho; Portugal
Fil: Bajón Fernández, Yadira. University Of Cranfield; Reino Unido
Fil: Batstone, Damien. The University of Queensland; Australia
Fil: Butler, Caitlyn. University of Massachussets; Estados Unidos
Fil: Cruz, Mercedes Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Davidsson, Åsa. Lund University; Suecia
Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Holliger, Christof. École Polytechnique Fédérale de Lausanne; Suiza
Fil: Koch, Konrad. Universitat Technical Zu Munich; Alemania
Fil: Kreuzinger, Norbert. Technische Universitat Wien; Austria
Fil: Lee, Changsoo. Ulsan National Institute Of Science And Technology; Corea del Sur
Fil: Lyberatos, Gerasimos. National Technical University of Athens; Grecia
Fil: Mutnuri, Srikanth. Birla Institute Of Technology And Science; India
Fil: O´Flaherty, Vincent. National University Of Ireland Galway.; Irlanda
Fil: Oleskowicz Popiel, Piotr. Poznan University Of Technology; Polonia
Fil: Pokorna, Dana. University of Chemistry and Technology Prague; República Checa
Fil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Recktenwald, Michael. Espoo R&D Center; Finlandia
Fil: Rodríguez, Jorge. Khalifa University; Emiratos Árabes Unidos
Fil: Saikaly, Pascal E.. King Abdullah University of Science and Technology; Arabia Saudita
Fil: Tooker, Nick. University of Massachussets; Estados Unidos
Fil: Vierheilig, Julia. Technische Universitat Wien; Austria
Fil: De Vrieze, Jo. University of Ghent; Bélgica
Fil: Wurzbacher, Christian. Technische Universitat München; Alemania
Fil: Nielsen, Per Halkjær. Aalborg University; Dinamarca