Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste

Autores
Orellana, Esteban; Davies Sala, Carol Giselle; Guerrero, Leandro Demián; Vardé, Ignacio; Altina, Melisa Guadalupe; Lorenzo, María Cielo; Figuerola, Eva Lucia Margarita; Pontiggia, Rodrigo Martin; Erijman, Leonardo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Addition of food waste (FW) as a co-substrate in anaerobic digesters of wastewater treatment plants is a desirable strategy towards achievement of the potential of wastewater treatment plants to become energy-neutral, diverting at the same time organic waste from landfills. Because substrate type is a driver of variations in phylogenetic structure of digester microbiomes, it is critical to understand how microbial communities respond to changes in substrate composition and concentration. In this work, high throughput sequencing was used to monitor the dynamics of microbiome changes in four parallel lab-scale anaerobic digesters treating sewage sludge during acclimation to an increasing amount of food waste. A co-occurrence network was constructed using data from 49 metagenomes sampled over the 161 days of the digesters´ operation. More than half of the nodes in the network were clustered in two major modules, i.e. groups of highly interconnected taxa that had much fewer connections with taxa outside the group. The dynamics of co-occurrence networks evidenced shifts that occurred within microbial communities due to the addition of food waste in the co-digestion process. A diverse and reproducible group of hydrolytic and fermentative bacteria, syntrophic bacteria and methanogenic archaea appeared to grow in a concerted fashion to allow stable performance of anaerobic co-digestion at high FW.
Fil: Orellana, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Davies Sala, Carol Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Guerrero, Leandro Demián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vardé, Ignacio. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; Argentina
Fil: Altina, Melisa Guadalupe. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; Argentina
Fil: Lorenzo, María Cielo. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; Argentina
Fil: Figuerola, Eva Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pontiggia, Rodrigo Martin. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
ANAEROBIC DIGESTION
CO-DIGESTION
FOOD WASTE
MICROBIOME
NETWORK ANALYSIS
SEWAGE SLUDGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104897
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/104897
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food wasteOrellana, EstebanDavies Sala, Carol GiselleGuerrero, Leandro DemiánVardé, IgnacioAltina, Melisa GuadalupeLorenzo, María CieloFiguerola, Eva Lucia MargaritaPontiggia, Rodrigo MartinErijman, LeonardoANAEROBIC DIGESTIONCO-DIGESTIONFOOD WASTEMICROBIOMENETWORK ANALYSISSEWAGE SLUDGEhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Addition of food waste (FW) as a co-substrate in anaerobic digesters of wastewater treatment plants is a desirable strategy towards achievement of the potential of wastewater treatment plants to become energy-neutral, diverting at the same time organic waste from landfills. Because substrate type is a driver of variations in phylogenetic structure of digester microbiomes, it is critical to understand how microbial communities respond to changes in substrate composition and concentration. In this work, high throughput sequencing was used to monitor the dynamics of microbiome changes in four parallel lab-scale anaerobic digesters treating sewage sludge during acclimation to an increasing amount of food waste. A co-occurrence network was constructed using data from 49 metagenomes sampled over the 161 days of the digesters´ operation. More than half of the nodes in the network were clustered in two major modules, i.e. groups of highly interconnected taxa that had much fewer connections with taxa outside the group. The dynamics of co-occurrence networks evidenced shifts that occurred within microbial communities due to the addition of food waste in the co-digestion process. A diverse and reproducible group of hydrolytic and fermentative bacteria, syntrophic bacteria and methanogenic archaea appeared to grow in a concerted fashion to allow stable performance of anaerobic co-digestion at high FW.Fil: Orellana, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Davies Sala, Carol Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Guerrero, Leandro Demián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Vardé, Ignacio. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; ArgentinaFil: Altina, Melisa Guadalupe. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; ArgentinaFil: Lorenzo, María Cielo. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; ArgentinaFil: Figuerola, Eva Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Pontiggia, Rodrigo Martin. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaIWA Publishing2019-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104897Orellana, Esteban; Davies Sala, Carol Giselle; Guerrero, Leandro Demián; Vardé, Ignacio; Altina, Melisa Guadalupe; et al.; Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste; IWA Publishing; Water Science And Technology; 79; 10; 15-6-2019; 1956-19650273-12231996-9732CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/wst/article/79/10/1956/67929/Microbiome-network-analysis-of-cooccurrenceinfo:eu-repo/semantics/altIdentifier/doi/10.2166/wst.2019.194info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:04Zoai:ri.conicet.gov.ar:11336/104897instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:04.458CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste
title Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste
spellingShingle Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste
Orellana, Esteban
ANAEROBIC DIGESTION
CO-DIGESTION
FOOD WASTE
MICROBIOME
NETWORK ANALYSIS
SEWAGE SLUDGE
title_short Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste
title_full Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste
title_fullStr Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste
title_full_unstemmed Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste
title_sort Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste
dc.creator.none.fl_str_mv Orellana, Esteban
Davies Sala, Carol Giselle
Guerrero, Leandro Demián
Vardé, Ignacio
Altina, Melisa Guadalupe
Lorenzo, María Cielo
Figuerola, Eva Lucia Margarita
Pontiggia, Rodrigo Martin
Erijman, Leonardo
author Orellana, Esteban
author_facet Orellana, Esteban
Davies Sala, Carol Giselle
Guerrero, Leandro Demián
Vardé, Ignacio
Altina, Melisa Guadalupe
Lorenzo, María Cielo
Figuerola, Eva Lucia Margarita
Pontiggia, Rodrigo Martin
Erijman, Leonardo
author_role author
author2 Davies Sala, Carol Giselle
Guerrero, Leandro Demián
Vardé, Ignacio
Altina, Melisa Guadalupe
Lorenzo, María Cielo
Figuerola, Eva Lucia Margarita
Pontiggia, Rodrigo Martin
Erijman, Leonardo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANAEROBIC DIGESTION
CO-DIGESTION
FOOD WASTE
MICROBIOME
NETWORK ANALYSIS
SEWAGE SLUDGE
topic ANAEROBIC DIGESTION
CO-DIGESTION
FOOD WASTE
MICROBIOME
NETWORK ANALYSIS
SEWAGE SLUDGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Addition of food waste (FW) as a co-substrate in anaerobic digesters of wastewater treatment plants is a desirable strategy towards achievement of the potential of wastewater treatment plants to become energy-neutral, diverting at the same time organic waste from landfills. Because substrate type is a driver of variations in phylogenetic structure of digester microbiomes, it is critical to understand how microbial communities respond to changes in substrate composition and concentration. In this work, high throughput sequencing was used to monitor the dynamics of microbiome changes in four parallel lab-scale anaerobic digesters treating sewage sludge during acclimation to an increasing amount of food waste. A co-occurrence network was constructed using data from 49 metagenomes sampled over the 161 days of the digesters´ operation. More than half of the nodes in the network were clustered in two major modules, i.e. groups of highly interconnected taxa that had much fewer connections with taxa outside the group. The dynamics of co-occurrence networks evidenced shifts that occurred within microbial communities due to the addition of food waste in the co-digestion process. A diverse and reproducible group of hydrolytic and fermentative bacteria, syntrophic bacteria and methanogenic archaea appeared to grow in a concerted fashion to allow stable performance of anaerobic co-digestion at high FW.
Fil: Orellana, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Davies Sala, Carol Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Guerrero, Leandro Demián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vardé, Ignacio. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; Argentina
Fil: Altina, Melisa Guadalupe. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; Argentina
Fil: Lorenzo, María Cielo. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; Argentina
Fil: Figuerola, Eva Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pontiggia, Rodrigo Martin. Investigación, Desarrollo e Innovación. Benito Roggio Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
description Addition of food waste (FW) as a co-substrate in anaerobic digesters of wastewater treatment plants is a desirable strategy towards achievement of the potential of wastewater treatment plants to become energy-neutral, diverting at the same time organic waste from landfills. Because substrate type is a driver of variations in phylogenetic structure of digester microbiomes, it is critical to understand how microbial communities respond to changes in substrate composition and concentration. In this work, high throughput sequencing was used to monitor the dynamics of microbiome changes in four parallel lab-scale anaerobic digesters treating sewage sludge during acclimation to an increasing amount of food waste. A co-occurrence network was constructed using data from 49 metagenomes sampled over the 161 days of the digesters´ operation. More than half of the nodes in the network were clustered in two major modules, i.e. groups of highly interconnected taxa that had much fewer connections with taxa outside the group. The dynamics of co-occurrence networks evidenced shifts that occurred within microbial communities due to the addition of food waste in the co-digestion process. A diverse and reproducible group of hydrolytic and fermentative bacteria, syntrophic bacteria and methanogenic archaea appeared to grow in a concerted fashion to allow stable performance of anaerobic co-digestion at high FW.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104897
Orellana, Esteban; Davies Sala, Carol Giselle; Guerrero, Leandro Demián; Vardé, Ignacio; Altina, Melisa Guadalupe; et al.; Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste; IWA Publishing; Water Science And Technology; 79; 10; 15-6-2019; 1956-1965
0273-1223
1996-9732
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104897
identifier_str_mv Orellana, Esteban; Davies Sala, Carol Giselle; Guerrero, Leandro Demián; Vardé, Ignacio; Altina, Melisa Guadalupe; et al.; Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste; IWA Publishing; Water Science And Technology; 79; 10; 15-6-2019; 1956-1965
0273-1223
1996-9732
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/wst/article/79/10/1956/67929/Microbiome-network-analysis-of-cooccurrence
info:eu-repo/semantics/altIdentifier/doi/10.2166/wst.2019.194
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IWA Publishing
publisher.none.fl_str_mv IWA Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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