Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression

Autores
Maistriaux, Laurie C.; Laurent, Maxime J.; Jeanguenin, Linda; Alvarez Prado, Santiago; Nader, Joseph; Welcker, Claude; Charcosset, Alain; Tardieu, François; Nicolas, Stéphane D; Chaumont, François
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant aquaporins are involved in numerous physiological processes, such as cellular homeostasis, tissue hydraulics, transpiration, and nutrient supply, and are key players of the response to environmental cues. While varying expression patterns of aquaporin genes have been described across organs, developmental stages, and stress conditions, the underlying regulation mechanisms remain elusive. Hence, this work aimed to shed light on the expression variability of 4 plasma membrane intrinsic protein (PIP) genes in maize (Zea mays) leaves, and its genetic causes, through expression quantitative trait locus (eQTL) mapping across a 252-hybrid diversity panel. Significant genetic variability in PIP transcript abundance was observed to different extents depending on the isoforms. The genomewide association study mapped numerous eQTLs, both local and distant, thus emphasizing the existing natural diversity of PIP gene expression across the studied panel and the potential to reveal regulatory actors and mechanisms. One eQTL associated with PIP2;5 expression variation was characterized. Genomic sequence comparison and in vivo reporter assay attributed, at least partly, the local eQTL to a transposon-containing polymorphism in the PIP2;5 promoter. This work paves the way to the molecular understanding of PIP gene regulation and its possible integration into larger networks regulating physiological and stress adaptation processes.
Fil: Maistriaux, Laurie C.. Université Catholique de Louvain; Bélgica
Fil: Laurent, Maxime J.. Université Catholique de Louvain; Bélgica
Fil: Jeanguenin, Linda. Université Catholique de Louvain; Bélgica
Fil: Alvarez Prado, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Institut National de la Recherche Agronomique; Francia
Fil: Nader, Joseph. Université Catholique de Louvain; Bélgica
Fil: Welcker, Claude. Institut National de la Recherche Agronomique; Francia
Fil: Charcosset, Alain. Institut National de la Recherche Agronomique; Francia
Fil: Tardieu, François. Institut National de la Recherche Agronomique; Francia
Fil: Nicolas, Stéphane D. Institut National de la Recherche Agronomique; Francia
Fil: Chaumont, François. Université Catholique de Louvain; Bélgica
Materia
AQUAPORIns
WATER TRANSPORT
MAIZE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266559
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/266559
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expressionMaistriaux, Laurie C.Laurent, Maxime J.Jeanguenin, LindaAlvarez Prado, SantiagoNader, JosephWelcker, ClaudeCharcosset, AlainTardieu, FrançoisNicolas, Stéphane DChaumont, FrançoisAQUAPORInsWATER TRANSPORTMAIZEhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Plant aquaporins are involved in numerous physiological processes, such as cellular homeostasis, tissue hydraulics, transpiration, and nutrient supply, and are key players of the response to environmental cues. While varying expression patterns of aquaporin genes have been described across organs, developmental stages, and stress conditions, the underlying regulation mechanisms remain elusive. Hence, this work aimed to shed light on the expression variability of 4 plasma membrane intrinsic protein (PIP) genes in maize (Zea mays) leaves, and its genetic causes, through expression quantitative trait locus (eQTL) mapping across a 252-hybrid diversity panel. Significant genetic variability in PIP transcript abundance was observed to different extents depending on the isoforms. The genomewide association study mapped numerous eQTLs, both local and distant, thus emphasizing the existing natural diversity of PIP gene expression across the studied panel and the potential to reveal regulatory actors and mechanisms. One eQTL associated with PIP2;5 expression variation was characterized. Genomic sequence comparison and in vivo reporter assay attributed, at least partly, the local eQTL to a transposon-containing polymorphism in the PIP2;5 promoter. This work paves the way to the molecular understanding of PIP gene regulation and its possible integration into larger networks regulating physiological and stress adaptation processes.Fil: Maistriaux, Laurie C.. Université Catholique de Louvain; BélgicaFil: Laurent, Maxime J.. Université Catholique de Louvain; BélgicaFil: Jeanguenin, Linda. Université Catholique de Louvain; BélgicaFil: Alvarez Prado, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Institut National de la Recherche Agronomique; FranciaFil: Nader, Joseph. Université Catholique de Louvain; BélgicaFil: Welcker, Claude. Institut National de la Recherche Agronomique; FranciaFil: Charcosset, Alain. Institut National de la Recherche Agronomique; FranciaFil: Tardieu, François. Institut National de la Recherche Agronomique; FranciaFil: Nicolas, Stéphane D. Institut National de la Recherche Agronomique; FranciaFil: Chaumont, François. Université Catholique de Louvain; BélgicaAmerican Society of Plant Biologist2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266559Maistriaux, Laurie C.; Laurent, Maxime J.; Jeanguenin, Linda; Alvarez Prado, Santiago; Nader, Joseph; et al.; Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression; American Society of Plant Biologist; Plant Physiology; 196; 1; 9-2024; 368-3840032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/196/1/368/7688441info:eu-repo/semantics/altIdentifier/doi/10.1093/plphys/kiae326info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:31Zoai:ri.conicet.gov.ar:11336/266559instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:31.652CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression
title Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression
spellingShingle Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression
Maistriaux, Laurie C.
AQUAPORIns
WATER TRANSPORT
MAIZE
title_short Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression
title_full Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression
title_fullStr Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression
title_full_unstemmed Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression
title_sort Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression
dc.creator.none.fl_str_mv Maistriaux, Laurie C.
Laurent, Maxime J.
Jeanguenin, Linda
Alvarez Prado, Santiago
Nader, Joseph
Welcker, Claude
Charcosset, Alain
Tardieu, François
Nicolas, Stéphane D
Chaumont, François
author Maistriaux, Laurie C.
author_facet Maistriaux, Laurie C.
Laurent, Maxime J.
Jeanguenin, Linda
Alvarez Prado, Santiago
Nader, Joseph
Welcker, Claude
Charcosset, Alain
Tardieu, François
Nicolas, Stéphane D
Chaumont, François
author_role author
author2 Laurent, Maxime J.
Jeanguenin, Linda
Alvarez Prado, Santiago
Nader, Joseph
Welcker, Claude
Charcosset, Alain
Tardieu, François
Nicolas, Stéphane D
Chaumont, François
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AQUAPORIns
WATER TRANSPORT
MAIZE
topic AQUAPORIns
WATER TRANSPORT
MAIZE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Plant aquaporins are involved in numerous physiological processes, such as cellular homeostasis, tissue hydraulics, transpiration, and nutrient supply, and are key players of the response to environmental cues. While varying expression patterns of aquaporin genes have been described across organs, developmental stages, and stress conditions, the underlying regulation mechanisms remain elusive. Hence, this work aimed to shed light on the expression variability of 4 plasma membrane intrinsic protein (PIP) genes in maize (Zea mays) leaves, and its genetic causes, through expression quantitative trait locus (eQTL) mapping across a 252-hybrid diversity panel. Significant genetic variability in PIP transcript abundance was observed to different extents depending on the isoforms. The genomewide association study mapped numerous eQTLs, both local and distant, thus emphasizing the existing natural diversity of PIP gene expression across the studied panel and the potential to reveal regulatory actors and mechanisms. One eQTL associated with PIP2;5 expression variation was characterized. Genomic sequence comparison and in vivo reporter assay attributed, at least partly, the local eQTL to a transposon-containing polymorphism in the PIP2;5 promoter. This work paves the way to the molecular understanding of PIP gene regulation and its possible integration into larger networks regulating physiological and stress adaptation processes.
Fil: Maistriaux, Laurie C.. Université Catholique de Louvain; Bélgica
Fil: Laurent, Maxime J.. Université Catholique de Louvain; Bélgica
Fil: Jeanguenin, Linda. Université Catholique de Louvain; Bélgica
Fil: Alvarez Prado, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Institut National de la Recherche Agronomique; Francia
Fil: Nader, Joseph. Université Catholique de Louvain; Bélgica
Fil: Welcker, Claude. Institut National de la Recherche Agronomique; Francia
Fil: Charcosset, Alain. Institut National de la Recherche Agronomique; Francia
Fil: Tardieu, François. Institut National de la Recherche Agronomique; Francia
Fil: Nicolas, Stéphane D. Institut National de la Recherche Agronomique; Francia
Fil: Chaumont, François. Université Catholique de Louvain; Bélgica
description Plant aquaporins are involved in numerous physiological processes, such as cellular homeostasis, tissue hydraulics, transpiration, and nutrient supply, and are key players of the response to environmental cues. While varying expression patterns of aquaporin genes have been described across organs, developmental stages, and stress conditions, the underlying regulation mechanisms remain elusive. Hence, this work aimed to shed light on the expression variability of 4 plasma membrane intrinsic protein (PIP) genes in maize (Zea mays) leaves, and its genetic causes, through expression quantitative trait locus (eQTL) mapping across a 252-hybrid diversity panel. Significant genetic variability in PIP transcript abundance was observed to different extents depending on the isoforms. The genomewide association study mapped numerous eQTLs, both local and distant, thus emphasizing the existing natural diversity of PIP gene expression across the studied panel and the potential to reveal regulatory actors and mechanisms. One eQTL associated with PIP2;5 expression variation was characterized. Genomic sequence comparison and in vivo reporter assay attributed, at least partly, the local eQTL to a transposon-containing polymorphism in the PIP2;5 promoter. This work paves the way to the molecular understanding of PIP gene regulation and its possible integration into larger networks regulating physiological and stress adaptation processes.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266559
Maistriaux, Laurie C.; Laurent, Maxime J.; Jeanguenin, Linda; Alvarez Prado, Santiago; Nader, Joseph; et al.; Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression; American Society of Plant Biologist; Plant Physiology; 196; 1; 9-2024; 368-384
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266559
identifier_str_mv Maistriaux, Laurie C.; Laurent, Maxime J.; Jeanguenin, Linda; Alvarez Prado, Santiago; Nader, Joseph; et al.; Genetic variability of aquaporin expression in maize: From eQTLs to a MITE insertion regulating PIP2;5 expression; American Society of Plant Biologist; Plant Physiology; 196; 1; 9-2024; 368-384
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/196/1/368/7688441
info:eu-repo/semantics/altIdentifier/doi/10.1093/plphys/kiae326
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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