Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters

Autores
Comelli, Raul Nicolas; Seluy, Lisandro Gabriel; Isla, Miguel Angel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In bioethanol production processes, the media composition has an impact on product concentration, yields and the overall process economics. The main purpose of this research was to develop a low-cost mineral-based supplement for successful alcoholic fermentation in an attempt to provide an economically feasible alternative to produce bioethanol from novel sources, e.g., sugary industrial wastewaters. Statistical experimental designs were used to select essential nutrients for yeast fermentation, and its optimal concentrations were estimated by Response Surface Methodology. Fermentations were performed on synthetic media inoculated with 2.0 g/L of yeast, and the evolution of biomass, sugar, ethanol, CO2 and glycerol were monitored over time. A mix of salts [10.6 g/L (NH4)2HPO4; 6.4 g/L MgSO4.7H2O and 7.5 mg/L ZnSO4.7H2O] was found to be optimal. It led to the complete fermentation of the sugars in less than 12 h with an average ethanol yield of 0.42 gethanol/gsugar. A general C-balance indicated that no carbonaceous compounds different from biomass, ethanol, CO2 or glycerol were produced in significant amounts in the fermentation process. Similar results were obtained when soft drink wastewaters were tested to evaluate the potential industrial application of this supplement. The ethanol yields were very close to those obtained when yeast extract was used as the supplement, but the optimized mineral-based medium is six times cheaper, which favorably impacts the process economics and makes this supplement more attractive from an industrial viewpoint.
Fil: Comelli, Raul Nicolas. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Seluy, Lisandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Isla, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Materia
Ethanol
Fermentation
Waste Treatment
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9900
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewatersComelli, Raul NicolasSeluy, Lisandro GabrielIsla, Miguel AngelEthanolFermentationWaste Treatmenthttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2In bioethanol production processes, the media composition has an impact on product concentration, yields and the overall process economics. The main purpose of this research was to develop a low-cost mineral-based supplement for successful alcoholic fermentation in an attempt to provide an economically feasible alternative to produce bioethanol from novel sources, e.g., sugary industrial wastewaters. Statistical experimental designs were used to select essential nutrients for yeast fermentation, and its optimal concentrations were estimated by Response Surface Methodology. Fermentations were performed on synthetic media inoculated with 2.0 g/L of yeast, and the evolution of biomass, sugar, ethanol, CO2 and glycerol were monitored over time. A mix of salts [10.6 g/L (NH4)2HPO4; 6.4 g/L MgSO4.7H2O and 7.5 mg/L ZnSO4.7H2O] was found to be optimal. It led to the complete fermentation of the sugars in less than 12 h with an average ethanol yield of 0.42 gethanol/gsugar. A general C-balance indicated that no carbonaceous compounds different from biomass, ethanol, CO2 or glycerol were produced in significant amounts in the fermentation process. Similar results were obtained when soft drink wastewaters were tested to evaluate the potential industrial application of this supplement. The ethanol yields were very close to those obtained when yeast extract was used as the supplement, but the optimized mineral-based medium is six times cheaper, which favorably impacts the process economics and makes this supplement more attractive from an industrial viewpoint.Fil: Comelli, Raul Nicolas. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); ArgentinaFil: Seluy, Lisandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; ArgentinaFil: Isla, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; ArgentinaElsevier Science2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9900Comelli, Raul Nicolas; Seluy, Lisandro Gabriel; Isla, Miguel Angel; Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters; Elsevier Science; New Biotechnology; 33; 1; 9-2015; 107-1151871-6784enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.nbt.2015.09.001info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1871678415001533info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:50Zoai:ri.conicet.gov.ar:11336/9900instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:51.052CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters
title Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters
spellingShingle Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters
Comelli, Raul Nicolas
Ethanol
Fermentation
Waste Treatment
title_short Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters
title_full Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters
title_fullStr Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters
title_full_unstemmed Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters
title_sort Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters
dc.creator.none.fl_str_mv Comelli, Raul Nicolas
Seluy, Lisandro Gabriel
Isla, Miguel Angel
author Comelli, Raul Nicolas
author_facet Comelli, Raul Nicolas
Seluy, Lisandro Gabriel
Isla, Miguel Angel
author_role author
author2 Seluy, Lisandro Gabriel
Isla, Miguel Angel
author2_role author
author
dc.subject.none.fl_str_mv Ethanol
Fermentation
Waste Treatment
topic Ethanol
Fermentation
Waste Treatment
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In bioethanol production processes, the media composition has an impact on product concentration, yields and the overall process economics. The main purpose of this research was to develop a low-cost mineral-based supplement for successful alcoholic fermentation in an attempt to provide an economically feasible alternative to produce bioethanol from novel sources, e.g., sugary industrial wastewaters. Statistical experimental designs were used to select essential nutrients for yeast fermentation, and its optimal concentrations were estimated by Response Surface Methodology. Fermentations were performed on synthetic media inoculated with 2.0 g/L of yeast, and the evolution of biomass, sugar, ethanol, CO2 and glycerol were monitored over time. A mix of salts [10.6 g/L (NH4)2HPO4; 6.4 g/L MgSO4.7H2O and 7.5 mg/L ZnSO4.7H2O] was found to be optimal. It led to the complete fermentation of the sugars in less than 12 h with an average ethanol yield of 0.42 gethanol/gsugar. A general C-balance indicated that no carbonaceous compounds different from biomass, ethanol, CO2 or glycerol were produced in significant amounts in the fermentation process. Similar results were obtained when soft drink wastewaters were tested to evaluate the potential industrial application of this supplement. The ethanol yields were very close to those obtained when yeast extract was used as the supplement, but the optimized mineral-based medium is six times cheaper, which favorably impacts the process economics and makes this supplement more attractive from an industrial viewpoint.
Fil: Comelli, Raul Nicolas. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Seluy, Lisandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Isla, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
description In bioethanol production processes, the media composition has an impact on product concentration, yields and the overall process economics. The main purpose of this research was to develop a low-cost mineral-based supplement for successful alcoholic fermentation in an attempt to provide an economically feasible alternative to produce bioethanol from novel sources, e.g., sugary industrial wastewaters. Statistical experimental designs were used to select essential nutrients for yeast fermentation, and its optimal concentrations were estimated by Response Surface Methodology. Fermentations were performed on synthetic media inoculated with 2.0 g/L of yeast, and the evolution of biomass, sugar, ethanol, CO2 and glycerol were monitored over time. A mix of salts [10.6 g/L (NH4)2HPO4; 6.4 g/L MgSO4.7H2O and 7.5 mg/L ZnSO4.7H2O] was found to be optimal. It led to the complete fermentation of the sugars in less than 12 h with an average ethanol yield of 0.42 gethanol/gsugar. A general C-balance indicated that no carbonaceous compounds different from biomass, ethanol, CO2 or glycerol were produced in significant amounts in the fermentation process. Similar results were obtained when soft drink wastewaters were tested to evaluate the potential industrial application of this supplement. The ethanol yields were very close to those obtained when yeast extract was used as the supplement, but the optimized mineral-based medium is six times cheaper, which favorably impacts the process economics and makes this supplement more attractive from an industrial viewpoint.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9900
Comelli, Raul Nicolas; Seluy, Lisandro Gabriel; Isla, Miguel Angel; Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters; Elsevier Science; New Biotechnology; 33; 1; 9-2015; 107-115
1871-6784
url http://hdl.handle.net/11336/9900
identifier_str_mv Comelli, Raul Nicolas; Seluy, Lisandro Gabriel; Isla, Miguel Angel; Optimization of a low-cost defined medium for alcoholic fermentation – a case study for potential application in bioethanol production from industrial wastewaters; Elsevier Science; New Biotechnology; 33; 1; 9-2015; 107-115
1871-6784
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nbt.2015.09.001
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1871678415001533
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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