Cryopreservation of Arachis pintoi seeds

Autores
Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Studies on the storage of seeds of the diploid cytotype (2n=2x= 20 chromosomes) of Arachis pintoi (Leguminosae) were conducted to preserve germplasm for the long term in liquid nitrogen (-196°C). The best cryo-procedure comprised: desiccation of the seeds to 4% water content (14 days at 40°C) and, subsequently, direct cooling in nitrogen liquid. The cryotubes were then rapidly warmed in a water bath at 38°C. With this treatment, after 7 days in a germinator up to 90% of the seeds germinated
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Materia
Arachis Pintoi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47346
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Cryopreservation of Arachis pintoi seedsRey de Badaró, Hebe YolandaMroginski, Luis AmadoArachis Pintoihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Studies on the storage of seeds of the diploid cytotype (2n=2x= 20 chromosomes) of Arachis pintoi (Leguminosae) were conducted to preserve germplasm for the long term in liquid nitrogen (-196°C). The best cryo-procedure comprised: desiccation of the seeds to 4% water content (14 days at 40°C) and, subsequently, direct cooling in nitrogen liquid. The cryotubes were then rapidly warmed in a water bath at 38°C. With this treatment, after 7 days in a germinator up to 90% of the seeds germinatedFil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaInternational Seed Testing Association2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47346Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; Cryopreservation of Arachis pintoi seeds; International Seed Testing Association; Seed Science And Technology; 37; 1; 12-2009; 202-2050251-09521819-5717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.15258/sst.2009.37.1.22info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/ista/sst/2009/00000037/00000001/art00022info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:05:56Zoai:ri.conicet.gov.ar:11336/47346instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:05:56.49CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cryopreservation of Arachis pintoi seeds
title Cryopreservation of Arachis pintoi seeds
spellingShingle Cryopreservation of Arachis pintoi seeds
Rey de Badaró, Hebe Yolanda
Arachis Pintoi
title_short Cryopreservation of Arachis pintoi seeds
title_full Cryopreservation of Arachis pintoi seeds
title_fullStr Cryopreservation of Arachis pintoi seeds
title_full_unstemmed Cryopreservation of Arachis pintoi seeds
title_sort Cryopreservation of Arachis pintoi seeds
dc.creator.none.fl_str_mv Rey de Badaró, Hebe Yolanda
Mroginski, Luis Amado
author Rey de Badaró, Hebe Yolanda
author_facet Rey de Badaró, Hebe Yolanda
Mroginski, Luis Amado
author_role author
author2 Mroginski, Luis Amado
author2_role author
dc.subject.none.fl_str_mv Arachis Pintoi
topic Arachis Pintoi
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Studies on the storage of seeds of the diploid cytotype (2n=2x= 20 chromosomes) of Arachis pintoi (Leguminosae) were conducted to preserve germplasm for the long term in liquid nitrogen (-196°C). The best cryo-procedure comprised: desiccation of the seeds to 4% water content (14 days at 40°C) and, subsequently, direct cooling in nitrogen liquid. The cryotubes were then rapidly warmed in a water bath at 38°C. With this treatment, after 7 days in a germinator up to 90% of the seeds germinated
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
description Studies on the storage of seeds of the diploid cytotype (2n=2x= 20 chromosomes) of Arachis pintoi (Leguminosae) were conducted to preserve germplasm for the long term in liquid nitrogen (-196°C). The best cryo-procedure comprised: desiccation of the seeds to 4% water content (14 days at 40°C) and, subsequently, direct cooling in nitrogen liquid. The cryotubes were then rapidly warmed in a water bath at 38°C. With this treatment, after 7 days in a germinator up to 90% of the seeds germinated
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47346
Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; Cryopreservation of Arachis pintoi seeds; International Seed Testing Association; Seed Science And Technology; 37; 1; 12-2009; 202-205
0251-0952
1819-5717
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47346
identifier_str_mv Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; Cryopreservation of Arachis pintoi seeds; International Seed Testing Association; Seed Science And Technology; 37; 1; 12-2009; 202-205
0251-0952
1819-5717
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.15258/sst.2009.37.1.22
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/ista/sst/2009/00000037/00000001/art00022
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Seed Testing Association
publisher.none.fl_str_mv International Seed Testing Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847977550432698368
score 13.087074

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